Experiment: m.b. Gentamicin 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0064 and B158DRAFT_0065 are separated by 164 nucleotides B158DRAFT_0065 and B158DRAFT_0066 are separated by 223 nucleotides B158DRAFT_0066 and B158DRAFT_0067 overlap by 20 nucleotides
B158DRAFT_0064: B158DRAFT_0064 - Uncharacterized protein conserved in bacteria, at 61,879 to 62,751
_0064
B158DRAFT_0065: B158DRAFT_0065 - hypothetical protein, at 62,916 to 63,422
_0065
B158DRAFT_0066: B158DRAFT_0066 - two component transcriptional regulator, winged helix family, at 63,646 to 64,344
_0066
B158DRAFT_0067: B158DRAFT_0067 - Signal transduction histidine kinase, at 64,325 to 65,728
_0067
Position (kb) 62
63
64 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 61.921 kb on + strand at 62.105 kb on + strand, within B158DRAFT_0064 at 62.113 kb on - strand, within B158DRAFT_0064 at 62.113 kb on - strand, within B158DRAFT_0064 at 62.126 kb on + strand, within B158DRAFT_0064 at 62.134 kb on - strand, within B158DRAFT_0064 at 62.165 kb on - strand, within B158DRAFT_0064 at 62.232 kb on - strand, within B158DRAFT_0064 at 62.259 kb on + strand, within B158DRAFT_0064 at 62.285 kb on + strand, within B158DRAFT_0064 at 62.301 kb on + strand, within B158DRAFT_0064 at 62.349 kb on + strand, within B158DRAFT_0064 at 62.349 kb on + strand, within B158DRAFT_0064 at 62.349 kb on + strand, within B158DRAFT_0064 at 62.349 kb on + strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.357 kb on - strand, within B158DRAFT_0064 at 62.451 kb on + strand, within B158DRAFT_0064 at 62.510 kb on - strand, within B158DRAFT_0064 at 62.547 kb on - strand, within B158DRAFT_0064 at 62.549 kb on + strand, within B158DRAFT_0064 at 62.557 kb on - strand, within B158DRAFT_0064 at 62.559 kb on + strand, within B158DRAFT_0064 at 62.579 kb on - strand, within B158DRAFT_0064 at 62.642 kb on + strand, within B158DRAFT_0064 at 62.650 kb on - strand, within B158DRAFT_0064 at 62.650 kb on - strand, within B158DRAFT_0064 at 62.702 kb on - strand at 62.753 kb on + strand at 62.873 kb on + strand at 63.050 kb on - strand, within B158DRAFT_0065 at 63.054 kb on + strand, within B158DRAFT_0065 at 63.062 kb on + strand, within B158DRAFT_0065 at 63.083 kb on + strand, within B158DRAFT_0065 at 63.157 kb on + strand, within B158DRAFT_0065 at 63.202 kb on + strand, within B158DRAFT_0065 at 63.397 kb on - strand at 63.397 kb on - strand at 63.397 kb on - strand at 63.552 kb on + strand at 63.635 kb on - strand at 63.674 kb on + strand at 63.679 kb on + strand at 63.679 kb on + strand at 63.692 kb on - strand at 63.745 kb on + strand, within B158DRAFT_0066 at 63.787 kb on - strand, within B158DRAFT_0066 at 63.800 kb on + strand, within B158DRAFT_0066 at 63.824 kb on + strand, within B158DRAFT_0066 at 63.956 kb on - strand, within B158DRAFT_0066 at 63.996 kb on - strand, within B158DRAFT_0066 at 64.086 kb on - strand, within B158DRAFT_0066 at 64.199 kb on - strand, within B158DRAFT_0066 at 64.237 kb on - strand, within B158DRAFT_0066 at 64.293 kb on + strand at 64.301 kb on - strand at 64.301 kb on - strand at 64.301 kb on - strand at 64.397 kb on + strand at 64.406 kb on + strand at 64.406 kb on + strand at 64.409 kb on - strand at 64.414 kb on - strand at 64.414 kb on - strand at 64.414 kb on - strand at 64.414 kb on - strand at 64.414 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Gentamicin 0.3 mg/ml remove 61,921 + +0.1 62,105 + B158DRAFT_0064 0.26 +0.5 62,113 - B158DRAFT_0064 0.27 -0.8 62,113 - B158DRAFT_0064 0.27 +0.4 62,126 + B158DRAFT_0064 0.28 +0.4 62,134 - B158DRAFT_0064 0.29 +1.1 62,165 - B158DRAFT_0064 0.33 +0.2 62,232 - B158DRAFT_0064 0.40 -0.5 62,259 + B158DRAFT_0064 0.44 +0.1 62,285 + B158DRAFT_0064 0.47 -0.6 62,301 + B158DRAFT_0064 0.48 -1.0 62,349 + B158DRAFT_0064 0.54 +0.0 62,349 + B158DRAFT_0064 0.54 -0.2 62,349 + B158DRAFT_0064 0.54 -0.5 62,349 + B158DRAFT_0064 0.54 -1.0 62,357 - B158DRAFT_0064 0.55 +1.2 62,357 - B158DRAFT_0064 0.55 +0.8 62,357 - B158DRAFT_0064 0.55 -0.4 62,357 - B158DRAFT_0064 0.55 +0.7 62,357 - B158DRAFT_0064 0.55 +0.2 62,357 - B158DRAFT_0064 0.55 +0.5 62,357 - B158DRAFT_0064 0.55 +0.4 62,451 + B158DRAFT_0064 0.66 +0.2 62,510 - B158DRAFT_0064 0.72 +0.9 62,547 - B158DRAFT_0064 0.77 +0.6 62,549 + B158DRAFT_0064 0.77 +0.5 62,557 - B158DRAFT_0064 0.78 +0.2 62,559 + B158DRAFT_0064 0.78 +0.3 62,579 - B158DRAFT_0064 0.80 +0.3 62,642 + B158DRAFT_0064 0.87 -0.3 62,650 - B158DRAFT_0064 0.88 -0.2 62,650 - B158DRAFT_0064 0.88 +0.5 62,702 - -0.4 62,753 + -0.4 62,873 + -0.2 63,050 - B158DRAFT_0065 0.26 -0.7 63,054 + B158DRAFT_0065 0.27 +1.0 63,062 + B158DRAFT_0065 0.29 +0.0 63,083 + B158DRAFT_0065 0.33 +0.7 63,157 + B158DRAFT_0065 0.48 +0.5 63,202 + B158DRAFT_0065 0.56 -1.1 63,397 - -0.2 63,397 - +0.4 63,397 - +0.0 63,552 + +0.3 63,635 - -0.4 63,674 + +0.5 63,679 + -0.9 63,679 + -0.4 63,692 - +0.3 63,745 + B158DRAFT_0066 0.14 -0.5 63,787 - B158DRAFT_0066 0.20 -0.5 63,800 + B158DRAFT_0066 0.22 -3.7 63,824 + B158DRAFT_0066 0.25 +0.2 63,956 - B158DRAFT_0066 0.44 -0.8 63,996 - B158DRAFT_0066 0.50 -1.9 64,086 - B158DRAFT_0066 0.63 +0.8 64,199 - B158DRAFT_0066 0.79 -1.2 64,237 - B158DRAFT_0066 0.85 +0.7 64,293 + +1.3 64,301 - -0.1 64,301 - +0.1 64,301 - -0.0 64,397 + +0.2 64,406 + -0.3 64,406 + -0.3 64,409 - -1.3 64,414 - +0.6 64,414 - +0.4 64,414 - -0.8 64,414 - -0.3 64,414 - +0.2
Or see this region's nucleotide sequence