Experiment: m.b. Doxycycline hyclate 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2023 and B158DRAFT_2024 are separated by 69 nucleotides B158DRAFT_2024 and B158DRAFT_2025 are separated by 76 nucleotides B158DRAFT_2025 and B158DRAFT_2026 are separated by 41 nucleotides
B158DRAFT_2023: B158DRAFT_2023 - Zn-dependent hydrolases, including glyoxylases, at 153,250 to 154,173
_2023
B158DRAFT_2024: B158DRAFT_2024 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 154,243 to 154,977
_2024
B158DRAFT_2025: B158DRAFT_2025 - Uncharacterized protein conserved in bacteria, at 155,054 to 155,488
_2025
B158DRAFT_2026: B158DRAFT_2026 - Uncharacterized protein conserved in bacteria, at 155,530 to 156,549
_2026
Position (kb) 154
155 Strain fitness (log2 ratio) -1
0
1 at 153.315 kb on - strand at 153.326 kb on - strand at 153.336 kb on - strand at 153.350 kb on + strand, within B158DRAFT_2023 at 153.353 kb on - strand, within B158DRAFT_2023 at 153.357 kb on - strand, within B158DRAFT_2023 at 153.376 kb on + strand, within B158DRAFT_2023 at 153.381 kb on + strand, within B158DRAFT_2023 at 153.435 kb on + strand, within B158DRAFT_2023 at 153.480 kb on - strand, within B158DRAFT_2023 at 153.613 kb on - strand, within B158DRAFT_2023 at 153.709 kb on + strand, within B158DRAFT_2023 at 153.734 kb on + strand, within B158DRAFT_2023 at 153.742 kb on - strand, within B158DRAFT_2023 at 153.742 kb on - strand, within B158DRAFT_2023 at 153.780 kb on + strand, within B158DRAFT_2023 at 153.782 kb on - strand, within B158DRAFT_2023 at 153.788 kb on - strand, within B158DRAFT_2023 at 153.788 kb on - strand, within B158DRAFT_2023 at 153.826 kb on + strand, within B158DRAFT_2023 at 153.831 kb on + strand, within B158DRAFT_2023 at 153.834 kb on - strand, within B158DRAFT_2023 at 153.874 kb on + strand, within B158DRAFT_2023 at 153.903 kb on - strand, within B158DRAFT_2023 at 153.973 kb on + strand, within B158DRAFT_2023 at 154.168 kb on + strand at 154.177 kb on + strand at 154.185 kb on - strand at 154.273 kb on + strand at 154.315 kb on + strand at 154.322 kb on - strand, within B158DRAFT_2024 at 154.322 kb on - strand, within B158DRAFT_2024 at 154.322 kb on - strand, within B158DRAFT_2024 at 154.323 kb on + strand, within B158DRAFT_2024 at 154.545 kb on + strand, within B158DRAFT_2024 at 154.597 kb on - strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.605 kb on + strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.640 kb on - strand, within B158DRAFT_2024 at 154.649 kb on - strand, within B158DRAFT_2024 at 154.733 kb on - strand, within B158DRAFT_2024 at 154.748 kb on + strand, within B158DRAFT_2024 at 154.784 kb on + strand, within B158DRAFT_2024 at 154.794 kb on + strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.803 kb on - strand, within B158DRAFT_2024 at 154.813 kb on + strand, within B158DRAFT_2024 at 154.813 kb on + strand, within B158DRAFT_2024 at 154.821 kb on - strand, within B158DRAFT_2024 at 154.828 kb on + strand, within B158DRAFT_2024 at 154.831 kb on - strand, within B158DRAFT_2024 at 154.841 kb on + strand, within B158DRAFT_2024 at 154.932 kb on + strand at 155.000 kb on - strand at 155.010 kb on + strand at 155.187 kb on - strand, within B158DRAFT_2025 at 155.256 kb on + strand, within B158DRAFT_2025 at 155.256 kb on + strand, within B158DRAFT_2025 at 155.256 kb on + strand, within B158DRAFT_2025 at 155.280 kb on + strand, within B158DRAFT_2025 at 155.310 kb on - strand, within B158DRAFT_2025 at 155.385 kb on + strand, within B158DRAFT_2025 at 155.385 kb on + strand, within B158DRAFT_2025 at 155.385 kb on + strand, within B158DRAFT_2025 at 155.393 kb on - strand, within B158DRAFT_2025 at 155.393 kb on - strand, within B158DRAFT_2025 at 155.397 kb on + strand, within B158DRAFT_2025 at 155.408 kb on + strand, within B158DRAFT_2025 at 155.415 kb on - strand, within B158DRAFT_2025 at 155.463 kb on + strand at 155.492 kb on - strand at 155.502 kb on - strand at 155.517 kb on - strand at 155.517 kb on - strand at 155.532 kb on + strand at 155.573 kb on + strand at 155.581 kb on - strand at 155.677 kb on + strand, within B158DRAFT_2026 at 155.687 kb on + strand, within B158DRAFT_2026 at 155.687 kb on + strand, within B158DRAFT_2026 at 155.687 kb on + strand, within B158DRAFT_2026 at 155.695 kb on - strand, within B158DRAFT_2026 at 155.695 kb on - strand, within B158DRAFT_2026 at 155.893 kb on + strand, within B158DRAFT_2026 at 155.894 kb on - strand, within B158DRAFT_2026 at 155.918 kb on - strand, within B158DRAFT_2026
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.03125 mg/ml remove 153,315 - +0.3 153,326 - -0.5 153,336 - +1.0 153,350 + B158DRAFT_2023 0.11 +0.1 153,353 - B158DRAFT_2023 0.11 +0.6 153,357 - B158DRAFT_2023 0.12 -0.3 153,376 + B158DRAFT_2023 0.14 +0.6 153,381 + B158DRAFT_2023 0.14 -0.3 153,435 + B158DRAFT_2023 0.20 -0.0 153,480 - B158DRAFT_2023 0.25 +0.5 153,613 - B158DRAFT_2023 0.39 -0.3 153,709 + B158DRAFT_2023 0.50 +1.1 153,734 + B158DRAFT_2023 0.52 -0.2 153,742 - B158DRAFT_2023 0.53 +0.2 153,742 - B158DRAFT_2023 0.53 +0.3 153,780 + B158DRAFT_2023 0.57 +0.3 153,782 - B158DRAFT_2023 0.58 -0.2 153,788 - B158DRAFT_2023 0.58 -0.1 153,788 - B158DRAFT_2023 0.58 -1.1 153,826 + B158DRAFT_2023 0.62 -0.4 153,831 + B158DRAFT_2023 0.63 -0.2 153,834 - B158DRAFT_2023 0.63 -0.2 153,874 + B158DRAFT_2023 0.68 +0.8 153,903 - B158DRAFT_2023 0.71 +0.7 153,973 + B158DRAFT_2023 0.78 -0.1 154,168 + -0.1 154,177 + -0.5 154,185 - -0.0 154,273 + +1.1 154,315 + +0.8 154,322 - B158DRAFT_2024 0.11 -0.4 154,322 - B158DRAFT_2024 0.11 -0.4 154,322 - B158DRAFT_2024 0.11 -0.1 154,323 + B158DRAFT_2024 0.11 -0.8 154,545 + B158DRAFT_2024 0.41 -0.5 154,597 - B158DRAFT_2024 0.48 -0.1 154,599 + B158DRAFT_2024 0.48 -0.1 154,599 + B158DRAFT_2024 0.48 +0.3 154,599 + B158DRAFT_2024 0.48 +0.2 154,599 + B158DRAFT_2024 0.48 +0.5 154,599 + B158DRAFT_2024 0.48 -1.1 154,599 + B158DRAFT_2024 0.48 -0.4 154,605 + B158DRAFT_2024 0.49 -1.0 154,607 - B158DRAFT_2024 0.50 +0.2 154,607 - B158DRAFT_2024 0.50 -0.6 154,607 - B158DRAFT_2024 0.50 -0.4 154,640 - B158DRAFT_2024 0.54 -1.2 154,649 - B158DRAFT_2024 0.55 -0.3 154,733 - B158DRAFT_2024 0.67 -0.2 154,748 + B158DRAFT_2024 0.69 -0.1 154,784 + B158DRAFT_2024 0.74 -0.6 154,794 + B158DRAFT_2024 0.75 -0.2 154,802 - B158DRAFT_2024 0.76 +0.1 154,802 - B158DRAFT_2024 0.76 +0.2 154,802 - B158DRAFT_2024 0.76 -0.1 154,803 - B158DRAFT_2024 0.76 +0.9 154,813 + B158DRAFT_2024 0.78 +0.2 154,813 + B158DRAFT_2024 0.78 -0.2 154,821 - B158DRAFT_2024 0.79 -0.8 154,828 + B158DRAFT_2024 0.80 -0.3 154,831 - B158DRAFT_2024 0.80 +0.2 154,841 + B158DRAFT_2024 0.81 -0.6 154,932 + -0.0 155,000 - +0.3 155,010 + +0.0 155,187 - B158DRAFT_2025 0.31 +0.2 155,256 + B158DRAFT_2025 0.46 +0.2 155,256 + B158DRAFT_2025 0.46 -0.4 155,256 + B158DRAFT_2025 0.46 -0.1 155,280 + B158DRAFT_2025 0.52 -0.6 155,310 - B158DRAFT_2025 0.59 +0.3 155,385 + B158DRAFT_2025 0.76 +0.4 155,385 + B158DRAFT_2025 0.76 +0.1 155,385 + B158DRAFT_2025 0.76 +0.3 155,393 - B158DRAFT_2025 0.78 +0.1 155,393 - B158DRAFT_2025 0.78 -0.1 155,397 + B158DRAFT_2025 0.79 -0.1 155,408 + B158DRAFT_2025 0.81 -0.0 155,415 - B158DRAFT_2025 0.83 -0.1 155,463 + -0.1 155,492 - +0.5 155,502 - -0.3 155,517 - +0.2 155,517 - -0.3 155,532 + -0.5 155,573 + -0.2 155,581 - -0.0 155,677 + B158DRAFT_2026 0.14 -1.0 155,687 + B158DRAFT_2026 0.15 -0.3 155,687 + B158DRAFT_2026 0.15 +0.4 155,687 + B158DRAFT_2026 0.15 -0.8 155,695 - B158DRAFT_2026 0.16 +0.1 155,695 - B158DRAFT_2026 0.16 -0.6 155,893 + B158DRAFT_2026 0.36 -0.2 155,894 - B158DRAFT_2026 0.36 +1.7 155,918 - B158DRAFT_2026 0.38 -0.2
Or see this region's nucleotide sequence