Experiment: m.b. Doxycycline hyclate 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2077 and B158DRAFT_2078 overlap by 4 nucleotides B158DRAFT_2078 and B158DRAFT_2079 are separated by 4 nucleotides B158DRAFT_2079 and B158DRAFT_2080 are separated by 26 nucleotides B158DRAFT_2080 and B158DRAFT_2081 are separated by 206 nucleotides B158DRAFT_2081 and B158DRAFT_2082 overlap by 4 nucleotides
B158DRAFT_2077: B158DRAFT_2077 - Predicted P-loop-containing kinase, at 217,415 to 218,266
_2077
B158DRAFT_2078: B158DRAFT_2078 - PTS IIA-like nitrogen-regulatory protein PtsN, at 218,263 to 218,811
_2078
B158DRAFT_2079: B158DRAFT_2079 - ribosomal subunit interface protein, at 218,816 to 219,103
_2079
B158DRAFT_2080: B158DRAFT_2080 - RNA polymerase, sigma 54 subunit, RpoN/SigL, at 219,130 to 220,602
_2080
B158DRAFT_2081: B158DRAFT_2081 - ABC-type (unclassified) transport system, ATPase component, at 220,809 to 221,534
_2081
B158DRAFT_2082: B158DRAFT_2082 - Uncharacterized protein conserved in bacteria, at 221,531 to 222,292
_2082
Position (kb) 219
220
221 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 218.131 kb on + strand, within B158DRAFT_2077 at 218.131 kb on + strand, within B158DRAFT_2077 at 218.139 kb on - strand, within B158DRAFT_2077 at 218.228 kb on + strand at 218.236 kb on - strand at 218.330 kb on - strand, within B158DRAFT_2078 at 218.340 kb on - strand, within B158DRAFT_2078 at 218.346 kb on - strand, within B158DRAFT_2078 at 218.347 kb on - strand, within B158DRAFT_2078 at 218.388 kb on + strand, within B158DRAFT_2078 at 218.406 kb on - strand, within B158DRAFT_2078 at 218.473 kb on - strand, within B158DRAFT_2078 at 218.475 kb on + strand, within B158DRAFT_2078 at 218.518 kb on + strand, within B158DRAFT_2078 at 218.518 kb on + strand, within B158DRAFT_2078 at 218.532 kb on + strand, within B158DRAFT_2078 at 218.532 kb on + strand, within B158DRAFT_2078 at 218.532 kb on + strand, within B158DRAFT_2078 at 218.573 kb on - strand, within B158DRAFT_2078 at 218.584 kb on + strand, within B158DRAFT_2078 at 218.586 kb on + strand, within B158DRAFT_2078 at 218.589 kb on + strand, within B158DRAFT_2078 at 218.589 kb on + strand, within B158DRAFT_2078 at 218.604 kb on - strand, within B158DRAFT_2078 at 218.604 kb on - strand, within B158DRAFT_2078 at 218.619 kb on - strand, within B158DRAFT_2078 at 218.653 kb on - strand, within B158DRAFT_2078 at 218.789 kb on - strand at 218.814 kb on + strand at 218.814 kb on + strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.822 kb on - strand at 218.891 kb on + strand, within B158DRAFT_2079 at 218.938 kb on + strand, within B158DRAFT_2079 at 219.103 kb on - strand at 219.154 kb on - strand at 219.264 kb on - strand at 219.264 kb on - strand at 219.292 kb on - strand, within B158DRAFT_2080 at 219.587 kb on - strand, within B158DRAFT_2080 at 219.587 kb on - strand, within B158DRAFT_2080 at 219.587 kb on - strand, within B158DRAFT_2080 at 219.605 kb on - strand, within B158DRAFT_2080 at 219.640 kb on - strand, within B158DRAFT_2080 at 219.689 kb on + strand, within B158DRAFT_2080 at 219.697 kb on - strand, within B158DRAFT_2080 at 219.697 kb on - strand, within B158DRAFT_2080 at 219.739 kb on - strand, within B158DRAFT_2080 at 219.795 kb on - strand, within B158DRAFT_2080 at 219.797 kb on + strand, within B158DRAFT_2080 at 220.195 kb on - strand, within B158DRAFT_2080 at 220.212 kb on - strand, within B158DRAFT_2080 at 220.314 kb on - strand, within B158DRAFT_2080 at 220.317 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.434 kb on + strand, within B158DRAFT_2080 at 220.442 kb on - strand, within B158DRAFT_2080 at 220.442 kb on - strand, within B158DRAFT_2080 at 220.510 kb on - strand at 220.542 kb on + strand at 220.661 kb on + strand at 220.765 kb on + strand at 220.773 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.0625 mg/ml remove 218,131 + B158DRAFT_2077 0.84 -2.5 218,131 + B158DRAFT_2077 0.84 -3.0 218,139 - B158DRAFT_2077 0.85 -3.7 218,228 + -0.2 218,236 - -2.4 218,330 - B158DRAFT_2078 0.12 -0.9 218,340 - B158DRAFT_2078 0.14 -0.3 218,346 - B158DRAFT_2078 0.15 -0.8 218,347 - B158DRAFT_2078 0.15 -0.6 218,388 + B158DRAFT_2078 0.23 -1.5 218,406 - B158DRAFT_2078 0.26 +0.1 218,473 - B158DRAFT_2078 0.38 -1.4 218,475 + B158DRAFT_2078 0.39 -1.0 218,518 + B158DRAFT_2078 0.46 -1.8 218,518 + B158DRAFT_2078 0.46 -1.3 218,532 + B158DRAFT_2078 0.49 -2.2 218,532 + B158DRAFT_2078 0.49 -0.7 218,532 + B158DRAFT_2078 0.49 -1.0 218,573 - B158DRAFT_2078 0.56 -1.3 218,584 + B158DRAFT_2078 0.58 -1.5 218,586 + B158DRAFT_2078 0.59 -2.1 218,589 + B158DRAFT_2078 0.59 -1.7 218,589 + B158DRAFT_2078 0.59 -1.1 218,604 - B158DRAFT_2078 0.62 -1.0 218,604 - B158DRAFT_2078 0.62 -1.5 218,619 - B158DRAFT_2078 0.65 -1.2 218,653 - B158DRAFT_2078 0.71 -0.5 218,789 - -1.1 218,814 + -0.5 218,814 + -0.5 218,822 - -0.8 218,822 - -0.5 218,822 - +0.0 218,822 - -0.5 218,822 - -0.3 218,822 - -1.8 218,891 + B158DRAFT_2079 0.26 -0.3 218,938 + B158DRAFT_2079 0.42 -0.2 219,103 - -0.1 219,154 - +0.0 219,264 - -0.1 219,264 - +0.1 219,292 - B158DRAFT_2080 0.11 -1.4 219,587 - B158DRAFT_2080 0.31 +0.8 219,587 - B158DRAFT_2080 0.31 -0.1 219,587 - B158DRAFT_2080 0.31 +0.2 219,605 - B158DRAFT_2080 0.32 -0.8 219,640 - B158DRAFT_2080 0.35 +0.0 219,689 + B158DRAFT_2080 0.38 -0.5 219,697 - B158DRAFT_2080 0.38 -0.5 219,697 - B158DRAFT_2080 0.38 -0.2 219,739 - B158DRAFT_2080 0.41 -0.3 219,795 - B158DRAFT_2080 0.45 +0.0 219,797 + B158DRAFT_2080 0.45 -0.2 220,195 - B158DRAFT_2080 0.72 +0.1 220,212 - B158DRAFT_2080 0.73 -0.2 220,314 - B158DRAFT_2080 0.80 -0.3 220,317 + B158DRAFT_2080 0.81 +0.3 220,434 + B158DRAFT_2080 0.89 +0.1 220,434 + B158DRAFT_2080 0.89 -0.2 220,434 + B158DRAFT_2080 0.89 +0.2 220,434 + B158DRAFT_2080 0.89 -0.3 220,434 + B158DRAFT_2080 0.89 -0.6 220,442 - B158DRAFT_2080 0.89 -0.0 220,442 - B158DRAFT_2080 0.89 +0.2 220,510 - +0.5 220,542 + -0.7 220,661 + +0.0 220,765 + -0.9 220,773 - -1.8
Or see this region's nucleotide sequence