Experiment: m.b. Dimethyl Sulfoxide 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2292 and B158DRAFT_2293 overlap by 11 nucleotides B158DRAFT_2293 and B158DRAFT_2294 are separated by 37 nucleotides B158DRAFT_2294 and B158DRAFT_2295 are separated by 1 nucleotides
B158DRAFT_2292: B158DRAFT_2292 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC 5.3.1.16)/phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 456,542 to 457,780
_2292
B158DRAFT_2293: B158DRAFT_2293 - histidinol dehydrogenase, at 457,770 to 458,987
_2293
B158DRAFT_2294: B158DRAFT_2294 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 459,025 to 459,513
_2294
B158DRAFT_2295: B158DRAFT_2295 - Uncharacterized conserved protein, at 459,515 to 460,579
_2295
Position (kb)
457
458
459 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 456.796 kb on - strand, within B158DRAFT_2292 at 456.810 kb on - strand, within B158DRAFT_2292 at 456.926 kb on - strand, within B158DRAFT_2292 at 456.939 kb on - strand, within B158DRAFT_2292 at 456.995 kb on + strand, within B158DRAFT_2292 at 456.997 kb on - strand, within B158DRAFT_2292 at 457.055 kb on - strand, within B158DRAFT_2292 at 457.055 kb on - strand, within B158DRAFT_2292 at 457.106 kb on + strand, within B158DRAFT_2292 at 457.114 kb on - strand, within B158DRAFT_2292 at 457.159 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.207 kb on - strand, within B158DRAFT_2292 at 457.255 kb on - strand, within B158DRAFT_2292 at 457.376 kb on - strand, within B158DRAFT_2292 at 457.410 kb on - strand, within B158DRAFT_2292 at 457.467 kb on - strand, within B158DRAFT_2292 at 457.560 kb on + strand, within B158DRAFT_2292 at 457.560 kb on + strand, within B158DRAFT_2292 at 457.568 kb on - strand, within B158DRAFT_2292 at 457.568 kb on - strand, within B158DRAFT_2292 at 457.583 kb on - strand, within B158DRAFT_2292 at 457.640 kb on + strand, within B158DRAFT_2292 at 457.658 kb on - strand at 457.728 kb on - strand at 457.765 kb on + strand at 457.803 kb on - strand at 457.831 kb on + strand at 457.831 kb on + strand at 457.831 kb on + strand at 457.839 kb on - strand at 457.924 kb on + strand, within B158DRAFT_2293 at 457.969 kb on + strand, within B158DRAFT_2293 at 458.072 kb on + strand, within B158DRAFT_2293 at 458.089 kb on + strand, within B158DRAFT_2293 at 458.287 kb on + strand, within B158DRAFT_2293 at 458.313 kb on + strand, within B158DRAFT_2293 at 458.329 kb on + strand, within B158DRAFT_2293 at 458.340 kb on + strand, within B158DRAFT_2293 at 458.348 kb on - strand, within B158DRAFT_2293 at 458.348 kb on - strand, within B158DRAFT_2293 at 458.348 kb on - strand, within B158DRAFT_2293 at 458.348 kb on - strand, within B158DRAFT_2293 at 458.418 kb on - strand, within B158DRAFT_2293 at 458.432 kb on - strand, within B158DRAFT_2293 at 458.432 kb on - strand, within B158DRAFT_2293 at 458.534 kb on + strand, within B158DRAFT_2293 at 458.535 kb on - strand, within B158DRAFT_2293 at 458.535 kb on - strand, within B158DRAFT_2293 at 458.563 kb on + strand, within B158DRAFT_2293 at 458.563 kb on + strand, within B158DRAFT_2293 at 458.563 kb on + strand, within B158DRAFT_2293 at 458.563 kb on + strand, within B158DRAFT_2293 at 458.571 kb on - strand, within B158DRAFT_2293 at 458.645 kb on + strand, within B158DRAFT_2293 at 458.645 kb on - strand, within B158DRAFT_2293 at 458.652 kb on + strand, within B158DRAFT_2293 at 458.675 kb on + strand, within B158DRAFT_2293 at 458.690 kb on + strand, within B158DRAFT_2293 at 458.690 kb on + strand, within B158DRAFT_2293 at 458.703 kb on + strand, within B158DRAFT_2293 at 458.733 kb on - strand, within B158DRAFT_2293 at 458.762 kb on - strand, within B158DRAFT_2293 at 458.801 kb on - strand, within B158DRAFT_2293 at 458.801 kb on - strand, within B158DRAFT_2293 at 458.801 kb on - strand, within B158DRAFT_2293 at 458.801 kb on - strand, within B158DRAFT_2293 at 458.801 kb on - strand, within B158DRAFT_2293 at 458.840 kb on - strand, within B158DRAFT_2293 at 458.850 kb on + strand, within B158DRAFT_2293 at 458.850 kb on + strand, within B158DRAFT_2293 at 458.850 kb on + strand, within B158DRAFT_2293 at 458.850 kb on + strand, within B158DRAFT_2293 at 458.850 kb on + strand, within B158DRAFT_2293 at 458.850 kb on + strand, within B158DRAFT_2293 at 458.850 kb on + strand, within B158DRAFT_2293 at 458.850 kb on + strand, within B158DRAFT_2293 at 458.858 kb on - strand, within B158DRAFT_2293 at 458.935 kb on - strand at 458.941 kb on - strand at 458.941 kb on - strand at 458.959 kb on - strand at 458.975 kb on + strand at 458.975 kb on + strand at 458.975 kb on + strand at 458.975 kb on + strand at 458.976 kb on - strand at 459.508 kb on + strand at 459.508 kb on + strand at 459.516 kb on - strand at 459.527 kb on + strand at 459.527 kb on + strand at 459.535 kb on - strand at 459.535 kb on - strand at 459.538 kb on + strand at 459.547 kb on - strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.557 kb on + strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.565 kb on - strand at 459.587 kb on - strand at 459.619 kb on + strand at 459.626 kb on - strand, within B158DRAFT_2295 at 459.627 kb on - strand, within B158DRAFT_2295 at 459.635 kb on - strand, within B158DRAFT_2295 at 459.691 kb on - strand, within B158DRAFT_2295 at 459.810 kb on + strand, within B158DRAFT_2295 at 459.810 kb on + strand, within B158DRAFT_2295 at 459.824 kb on + strand, within B158DRAFT_2295 at 459.955 kb on + strand, within B158DRAFT_2295 at 459.955 kb on + strand, within B158DRAFT_2295
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 2.5 vol% remove 456,796 - B158DRAFT_2292 0.21 -1.3 456,810 - B158DRAFT_2292 0.22 +0.6 456,926 - B158DRAFT_2292 0.31 -1.3 456,939 - B158DRAFT_2292 0.32 -0.7 456,995 + B158DRAFT_2292 0.37 +0.5 456,997 - B158DRAFT_2292 0.37 -1.8 457,055 - B158DRAFT_2292 0.41 -2.2 457,055 - B158DRAFT_2292 0.41 -0.9 457,106 + B158DRAFT_2292 0.46 +0.5 457,114 - B158DRAFT_2292 0.46 +0.2 457,159 - B158DRAFT_2292 0.50 -0.8 457,207 - B158DRAFT_2292 0.54 -1.1 457,207 - B158DRAFT_2292 0.54 -0.5 457,207 - B158DRAFT_2292 0.54 -1.0 457,207 - B158DRAFT_2292 0.54 -0.3 457,207 - B158DRAFT_2292 0.54 -0.5 457,255 - B158DRAFT_2292 0.58 -1.8 457,376 - B158DRAFT_2292 0.67 -0.0 457,410 - B158DRAFT_2292 0.70 -1.3 457,467 - B158DRAFT_2292 0.75 -1.3 457,560 + B158DRAFT_2292 0.82 -0.7 457,560 + B158DRAFT_2292 0.82 -0.1 457,568 - B158DRAFT_2292 0.83 +0.1 457,568 - B158DRAFT_2292 0.83 +1.0 457,583 - B158DRAFT_2292 0.84 +2.1 457,640 + B158DRAFT_2292 0.89 +0.8 457,658 - +1.0 457,728 - -1.6 457,765 + -0.8 457,803 - +1.0 457,831 + -0.1 457,831 + +0.8 457,831 + -0.8 457,839 - -1.1 457,924 + B158DRAFT_2293 0.13 +0.8 457,969 + B158DRAFT_2293 0.16 -0.1 458,072 + B158DRAFT_2293 0.25 +0.0 458,089 + B158DRAFT_2293 0.26 -1.0 458,287 + B158DRAFT_2293 0.42 -1.5 458,313 + B158DRAFT_2293 0.45 +0.0 458,329 + B158DRAFT_2293 0.46 -2.1 458,340 + B158DRAFT_2293 0.47 -0.5 458,348 - B158DRAFT_2293 0.47 -2.5 458,348 - B158DRAFT_2293 0.47 -1.6 458,348 - B158DRAFT_2293 0.47 +1.3 458,348 - B158DRAFT_2293 0.47 -0.7 458,418 - B158DRAFT_2293 0.53 -0.7 458,432 - B158DRAFT_2293 0.54 +1.3 458,432 - B158DRAFT_2293 0.54 -1.4 458,534 + B158DRAFT_2293 0.63 +0.3 458,535 - B158DRAFT_2293 0.63 +1.0 458,535 - B158DRAFT_2293 0.63 -1.1 458,563 + B158DRAFT_2293 0.65 +1.6 458,563 + B158DRAFT_2293 0.65 -0.7 458,563 + B158DRAFT_2293 0.65 -0.2 458,563 + B158DRAFT_2293 0.65 +0.7 458,571 - B158DRAFT_2293 0.66 +0.1 458,645 + B158DRAFT_2293 0.72 -1.4 458,645 - B158DRAFT_2293 0.72 +0.2 458,652 + B158DRAFT_2293 0.72 -1.6 458,675 + B158DRAFT_2293 0.74 -0.9 458,690 + B158DRAFT_2293 0.76 +0.4 458,690 + B158DRAFT_2293 0.76 +2.1 458,703 + B158DRAFT_2293 0.77 +0.3 458,733 - B158DRAFT_2293 0.79 +1.4 458,762 - B158DRAFT_2293 0.81 -1.5 458,801 - B158DRAFT_2293 0.85 +0.0 458,801 - B158DRAFT_2293 0.85 +0.4 458,801 - B158DRAFT_2293 0.85 -0.1 458,801 - B158DRAFT_2293 0.85 -0.8 458,801 - B158DRAFT_2293 0.85 -2.0 458,840 - B158DRAFT_2293 0.88 -0.1 458,850 + B158DRAFT_2293 0.89 +2.0 458,850 + B158DRAFT_2293 0.89 -0.2 458,850 + B158DRAFT_2293 0.89 -1.1 458,850 + B158DRAFT_2293 0.89 +0.2 458,850 + B158DRAFT_2293 0.89 +0.0 458,850 + B158DRAFT_2293 0.89 +1.1 458,850 + B158DRAFT_2293 0.89 -1.9 458,850 + B158DRAFT_2293 0.89 -1.7 458,858 - B158DRAFT_2293 0.89 +0.8 458,935 - -1.1 458,941 - -0.5 458,941 - -0.6 458,959 - -0.8 458,975 + +0.7 458,975 + +0.8 458,975 + -2.0 458,975 + +0.7 458,976 - -0.9 459,508 + -1.8 459,508 + -2.1 459,516 - -1.8 459,527 + -1.5 459,527 + -0.1 459,535 - -0.5 459,535 - -1.6 459,538 + +0.4 459,547 - -0.4 459,557 + -0.7 459,557 + -0.6 459,557 + -0.7 459,557 + -0.6 459,557 + -1.0 459,557 + -1.2 459,557 + -3.2 459,557 + -1.7 459,557 + -1.2 459,557 + -1.6 459,557 + -1.0 459,557 + +0.6 459,557 + +0.7 459,557 + -1.5 459,557 + -0.5 459,557 + +0.1 459,557 + -2.0 459,557 + +0.3 459,557 + +0.0 459,557 + +0.8 459,557 + -0.9 459,557 + -2.9 459,557 + -0.7 459,557 + +0.6 459,557 + +1.6 459,557 + -0.7 459,557 + +0.4 459,565 - +1.4 459,565 - +0.3 459,565 - -0.3 459,565 - -1.1 459,565 - -2.1 459,565 - -1.9 459,565 - -1.3 459,565 - -1.4 459,565 - -0.8 459,565 - -0.3 459,565 - -1.4 459,565 - -2.4 459,565 - -1.6 459,565 - -0.7 459,565 - -1.7 459,565 - -3.0 459,565 - -2.3 459,565 - -1.8 459,565 - +0.5 459,565 - -1.5 459,565 - +0.0 459,565 - -1.7 459,565 - -0.5 459,565 - -1.5 459,565 - -2.9 459,565 - +0.0 459,565 - +0.8 459,565 - -1.0 459,565 - -1.0 459,565 - -1.7 459,565 - -1.1 459,565 - -1.8 459,587 - -2.8 459,619 + -0.1 459,626 - B158DRAFT_2295 0.10 -2.2 459,627 - B158DRAFT_2295 0.11 -0.9 459,635 - B158DRAFT_2295 0.11 -1.4 459,691 - B158DRAFT_2295 0.17 -0.3 459,810 + B158DRAFT_2295 0.28 +0.7 459,810 + B158DRAFT_2295 0.28 -0.1 459,824 + B158DRAFT_2295 0.29 -1.7 459,955 + B158DRAFT_2295 0.41 -1.3 459,955 + B158DRAFT_2295 0.41 -1.5
Or see this region's nucleotide sequence