Experiment: m.b. Dimethyl Sulfoxide 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2052 and B158DRAFT_2053 are separated by 29 nucleotides B158DRAFT_2053 and B158DRAFT_2054 are separated by 125 nucleotides B158DRAFT_2054 and B158DRAFT_2055 are separated by 516 nucleotides
B158DRAFT_2052: B158DRAFT_2052 - GTP-binding protein YchF, at 185,914 to 187,005
_2052
B158DRAFT_2053: B158DRAFT_2053 - peptidyl-tRNA hydrolase, at 187,035 to 187,628
_2053
B158DRAFT_2054: B158DRAFT_2054 - ribosomal protein L25, Ctc-form, at 187,754 to 188,431
_2054
B158DRAFT_2055: B158DRAFT_2055 - DNA-binding winged-HTH domains, at 188,948 to 190,231
_2055
Position (kb) 187
188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 186.766 kb on - strand, within B158DRAFT_2052 at 186.869 kb on - strand, within B158DRAFT_2052 at 186.891 kb on + strand, within B158DRAFT_2052 at 186.899 kb on - strand at 186.910 kb on + strand at 186.944 kb on + strand at 186.944 kb on + strand at 186.945 kb on - strand at 186.949 kb on + strand at 186.952 kb on - strand at 186.962 kb on - strand at 187.014 kb on - strand at 187.049 kb on + strand at 187.049 kb on + strand at 187.057 kb on - strand at 187.057 kb on - strand at 187.075 kb on + strand at 187.075 kb on + strand at 187.075 kb on + strand at 187.075 kb on + strand at 187.075 kb on + strand at 187.075 kb on + strand at 187.083 kb on - strand at 187.083 kb on - strand at 187.101 kb on + strand, within B158DRAFT_2053 at 187.628 kb on - strand at 187.651 kb on - strand at 187.760 kb on - strand at 187.865 kb on - strand, within B158DRAFT_2054 at 187.889 kb on + strand, within B158DRAFT_2054 at 187.897 kb on - strand, within B158DRAFT_2054 at 187.957 kb on - strand, within B158DRAFT_2054 at 187.969 kb on + strand, within B158DRAFT_2054 at 187.969 kb on + strand, within B158DRAFT_2054 at 188.037 kb on - strand, within B158DRAFT_2054 at 188.102 kb on + strand, within B158DRAFT_2054 at 188.102 kb on + strand, within B158DRAFT_2054 at 188.693 kb on + strand at 188.693 kb on + strand at 188.693 kb on + strand at 188.693 kb on + strand at 188.701 kb on - strand at 188.701 kb on - strand at 188.701 kb on - strand at 188.701 kb on - strand at 188.701 kb on - strand at 188.701 kb on - strand at 188.758 kb on - strand at 188.796 kb on - strand at 188.893 kb on - strand at 188.933 kb on - strand at 188.937 kb on - strand at 188.990 kb on - strand at 189.016 kb on + strand at 189.022 kb on - strand at 189.024 kb on + strand at 189.024 kb on + strand at 189.024 kb on - strand at 189.032 kb on - strand at 189.039 kb on + strand at 189.047 kb on - strand at 189.076 kb on + strand at 189.083 kb on + strand, within B158DRAFT_2055 at 189.091 kb on - strand, within B158DRAFT_2055 at 189.091 kb on - strand, within B158DRAFT_2055 at 189.091 kb on - strand, within B158DRAFT_2055 at 189.091 kb on - strand, within B158DRAFT_2055 at 189.091 kb on - strand, within B158DRAFT_2055 at 189.091 kb on - strand, within B158DRAFT_2055 at 189.227 kb on + strand, within B158DRAFT_2055 at 189.264 kb on + strand, within B158DRAFT_2055 at 189.264 kb on + strand, within B158DRAFT_2055 at 189.272 kb on - strand, within B158DRAFT_2055 at 189.272 kb on - strand, within B158DRAFT_2055 at 189.324 kb on - strand, within B158DRAFT_2055 at 189.324 kb on - strand, within B158DRAFT_2055 at 189.375 kb on + strand, within B158DRAFT_2055 at 189.375 kb on + strand, within B158DRAFT_2055 at 189.375 kb on + strand, within B158DRAFT_2055 at 189.375 kb on + strand, within B158DRAFT_2055 at 189.375 kb on + strand, within B158DRAFT_2055 at 189.375 kb on + strand, within B158DRAFT_2055 at 189.380 kb on + strand, within B158DRAFT_2055 at 189.383 kb on - strand, within B158DRAFT_2055 at 189.383 kb on - strand, within B158DRAFT_2055 at 189.383 kb on - strand, within B158DRAFT_2055 at 189.383 kb on - strand, within B158DRAFT_2055 at 189.383 kb on - strand, within B158DRAFT_2055 at 189.400 kb on + strand, within B158DRAFT_2055 at 189.410 kb on - strand, within B158DRAFT_2055 at 189.410 kb on - strand, within B158DRAFT_2055 at 189.410 kb on - strand, within B158DRAFT_2055
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 2.5 vol% remove 186,766 - B158DRAFT_2052 0.78 +1.3 186,869 - B158DRAFT_2052 0.87 -2.4 186,891 + B158DRAFT_2052 0.89 -1.1 186,899 - -2.8 186,910 + +0.8 186,944 + -1.2 186,944 + -1.3 186,945 - +0.0 186,949 + +2.1 186,952 - +0.0 186,962 - +0.5 187,014 - -1.1 187,049 + -0.5 187,049 + +0.6 187,057 - -1.4 187,057 - +0.2 187,075 + +0.6 187,075 + -1.8 187,075 + +2.1 187,075 + -0.3 187,075 + -0.7 187,075 + -1.1 187,083 - -1.5 187,083 - +0.1 187,101 + B158DRAFT_2053 0.11 +0.4 187,628 - +0.8 187,651 - -0.6 187,760 - +2.5 187,865 - B158DRAFT_2054 0.16 +0.5 187,889 + B158DRAFT_2054 0.20 +0.9 187,897 - B158DRAFT_2054 0.21 -0.6 187,957 - B158DRAFT_2054 0.30 +1.6 187,969 + B158DRAFT_2054 0.32 +5.7 187,969 + B158DRAFT_2054 0.32 +0.6 188,037 - B158DRAFT_2054 0.42 +4.6 188,102 + B158DRAFT_2054 0.51 +1.3 188,102 + B158DRAFT_2054 0.51 +0.6 188,693 + -0.2 188,693 + +3.6 188,693 + +0.3 188,693 + -2.6 188,701 - -2.1 188,701 - -1.6 188,701 - +1.8 188,701 - -1.9 188,701 - -0.6 188,701 - -1.8 188,758 - +0.8 188,796 - -1.1 188,893 - -0.6 188,933 - +0.0 188,937 - +0.3 188,990 - -1.2 189,016 + -1.5 189,022 - -0.6 189,024 + -0.2 189,024 + +0.0 189,024 - +0.1 189,032 - +0.2 189,039 + -0.3 189,047 - +0.3 189,076 + -1.2 189,083 + B158DRAFT_2055 0.11 +2.1 189,091 - B158DRAFT_2055 0.11 -0.2 189,091 - B158DRAFT_2055 0.11 -0.5 189,091 - B158DRAFT_2055 0.11 -1.2 189,091 - B158DRAFT_2055 0.11 -1.1 189,091 - B158DRAFT_2055 0.11 +0.4 189,091 - B158DRAFT_2055 0.11 -0.5 189,227 + B158DRAFT_2055 0.22 -0.2 189,264 + B158DRAFT_2055 0.25 +1.0 189,264 + B158DRAFT_2055 0.25 -0.4 189,272 - B158DRAFT_2055 0.25 -1.9 189,272 - B158DRAFT_2055 0.25 -1.1 189,324 - B158DRAFT_2055 0.29 +0.8 189,324 - B158DRAFT_2055 0.29 -2.7 189,375 + B158DRAFT_2055 0.33 -1.3 189,375 + B158DRAFT_2055 0.33 -1.3 189,375 + B158DRAFT_2055 0.33 -0.9 189,375 + B158DRAFT_2055 0.33 -0.2 189,375 + B158DRAFT_2055 0.33 -0.3 189,375 + B158DRAFT_2055 0.33 -1.7 189,380 + B158DRAFT_2055 0.34 -0.7 189,383 - B158DRAFT_2055 0.34 -1.6 189,383 - B158DRAFT_2055 0.34 -0.1 189,383 - B158DRAFT_2055 0.34 +0.2 189,383 - B158DRAFT_2055 0.34 -0.5 189,383 - B158DRAFT_2055 0.34 -2.3 189,400 + B158DRAFT_2055 0.35 -1.6 189,410 - B158DRAFT_2055 0.36 -0.7 189,410 - B158DRAFT_2055 0.36 -0.2 189,410 - B158DRAFT_2055 0.36 +0.3
Or see this region's nucleotide sequence