Experiment: m.b. Dimethyl Sulfoxide 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1919 and B158DRAFT_1920 are separated by 12 nucleotides B158DRAFT_1920 and B158DRAFT_1921 are separated by 251 nucleotides B158DRAFT_1921 and B158DRAFT_1922 are separated by 300 nucleotides
B158DRAFT_1919: B158DRAFT_1919 - farnesyl-diphosphate synthase (EC 2.5.1.10), at 54,314 to 55,204
_1919
B158DRAFT_1920: B158DRAFT_1920 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 55,217 to 55,462
_1920
B158DRAFT_1921: B158DRAFT_1921 - glutaminyl-tRNA synthetase (EC 6.1.1.18), at 55,714 to 57,426
_1921
B158DRAFT_1922: B158DRAFT_1922 - Uncharacterized protein involved in outer membrane biogenesis, at 57,727 to 59,574
_1922
Position (kb) 55
56
57
58 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.264 kb on - strand, within B158DRAFT_1920 at 55.323 kb on - strand, within B158DRAFT_1920 at 55.344 kb on - strand, within B158DRAFT_1920 at 55.376 kb on - strand, within B158DRAFT_1920 at 55.511 kb on - strand at 55.511 kb on - strand at 55.618 kb on - strand at 57.396 kb on + strand at 57.396 kb on + strand at 57.396 kb on + strand at 57.444 kb on - strand at 57.457 kb on + strand at 57.505 kb on - strand at 57.505 kb on - strand at 57.506 kb on - strand at 57.506 kb on - strand at 57.507 kb on + strand at 57.515 kb on - strand at 57.515 kb on - strand at 57.515 kb on - strand at 57.515 kb on - strand at 57.515 kb on - strand at 57.517 kb on + strand at 57.517 kb on + strand at 57.521 kb on + strand at 57.525 kb on - strand at 57.525 kb on - strand at 57.525 kb on - strand at 57.530 kb on - strand at 57.531 kb on + strand at 57.539 kb on - strand at 57.539 kb on - strand at 57.553 kb on - strand at 57.608 kb on - strand at 57.646 kb on - strand at 57.683 kb on + strand at 57.757 kb on - strand at 57.757 kb on - strand at 57.757 kb on - strand at 57.828 kb on + strand at 57.828 kb on + strand at 57.836 kb on - strand at 57.841 kb on - strand at 57.892 kb on + strand at 57.893 kb on + strand at 57.900 kb on - strand at 57.900 kb on - strand at 57.955 kb on + strand, within B158DRAFT_1922 at 57.970 kb on - strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 58.018 kb on - strand, within B158DRAFT_1922 at 58.084 kb on - strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.167 kb on + strand, within B158DRAFT_1922 at 58.167 kb on + strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.175 kb on - strand, within B158DRAFT_1922 at 58.227 kb on + strand, within B158DRAFT_1922 at 58.235 kb on - strand, within B158DRAFT_1922 at 58.289 kb on - strand, within B158DRAFT_1922 at 58.317 kb on + strand, within B158DRAFT_1922 at 58.320 kb on - strand, within B158DRAFT_1922 at 58.335 kb on - strand, within B158DRAFT_1922 at 58.335 kb on - strand, within B158DRAFT_1922 at 58.354 kb on - strand, within B158DRAFT_1922 at 58.359 kb on - strand, within B158DRAFT_1922
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 2.5 vol% remove 55,216 - +0.6 55,216 - -0.9 55,216 - -2.0 55,216 - -0.8 55,216 - +1.7 55,216 - +0.2 55,216 - +0.2 55,216 - -1.2 55,216 - -2.4 55,216 - +0.4 55,216 - -0.2 55,264 - B158DRAFT_1920 0.19 +3.8 55,323 - B158DRAFT_1920 0.43 +5.1 55,344 - B158DRAFT_1920 0.52 +5.2 55,376 - B158DRAFT_1920 0.65 +5.0 55,511 - +6.0 55,511 - +5.2 55,618 - -1.5 57,396 + +0.7 57,396 + -0.7 57,396 + +0.5 57,444 - -1.8 57,457 + -1.1 57,505 - -2.3 57,505 - -0.4 57,506 - -0.6 57,506 - -0.3 57,507 + -2.7 57,515 - -0.4 57,515 - +0.0 57,515 - -2.2 57,515 - -0.1 57,515 - -1.2 57,517 + -1.3 57,517 + +0.6 57,521 + -0.2 57,525 - +0.2 57,525 - -2.5 57,525 - -2.0 57,530 - -1.1 57,531 + -2.3 57,539 - -1.0 57,539 - +0.5 57,553 - -0.5 57,608 - -0.5 57,646 - -0.6 57,683 + -0.4 57,757 - -0.8 57,757 - -0.8 57,757 - -0.2 57,828 + -2.4 57,828 + +0.8 57,836 - -0.1 57,841 - -0.4 57,892 + -2.4 57,893 + -0.1 57,900 - -2.3 57,900 - -1.5 57,955 + B158DRAFT_1922 0.12 -1.1 57,970 - B158DRAFT_1922 0.13 -0.6 57,972 + B158DRAFT_1922 0.13 -0.4 57,972 + B158DRAFT_1922 0.13 +0.2 57,972 + B158DRAFT_1922 0.13 -1.2 57,972 + B158DRAFT_1922 0.13 +0.4 57,972 + B158DRAFT_1922 0.13 -2.1 57,972 + B158DRAFT_1922 0.13 -1.8 57,972 + B158DRAFT_1922 0.13 -2.0 57,972 + B158DRAFT_1922 0.13 -0.4 57,972 + B158DRAFT_1922 0.13 -3.4 57,972 + B158DRAFT_1922 0.13 -2.2 57,972 + B158DRAFT_1922 0.13 -2.3 57,980 - B158DRAFT_1922 0.14 -1.1 57,980 - B158DRAFT_1922 0.14 +1.0 57,980 - B158DRAFT_1922 0.14 -1.5 57,980 - B158DRAFT_1922 0.14 -2.7 57,980 - B158DRAFT_1922 0.14 +1.5 57,980 - B158DRAFT_1922 0.14 -1.4 57,980 - B158DRAFT_1922 0.14 -0.8 57,980 - B158DRAFT_1922 0.14 -1.8 57,980 - B158DRAFT_1922 0.14 -1.4 58,018 - B158DRAFT_1922 0.16 +1.3 58,084 - B158DRAFT_1922 0.19 -0.3 58,162 + B158DRAFT_1922 0.24 -1.4 58,162 + B158DRAFT_1922 0.24 -0.4 58,162 + B158DRAFT_1922 0.24 -0.3 58,162 + B158DRAFT_1922 0.24 -1.4 58,162 + B158DRAFT_1922 0.24 +0.4 58,162 + B158DRAFT_1922 0.24 -2.1 58,167 + B158DRAFT_1922 0.24 +2.0 58,167 + B158DRAFT_1922 0.24 -1.1 58,170 - B158DRAFT_1922 0.24 -1.5 58,170 - B158DRAFT_1922 0.24 +0.4 58,170 - B158DRAFT_1922 0.24 +0.1 58,170 - B158DRAFT_1922 0.24 +0.3 58,170 - B158DRAFT_1922 0.24 -1.7 58,170 - B158DRAFT_1922 0.24 -0.3 58,175 - B158DRAFT_1922 0.24 -0.2 58,227 + B158DRAFT_1922 0.27 +0.8 58,235 - B158DRAFT_1922 0.27 -0.4 58,289 - B158DRAFT_1922 0.30 -2.0 58,317 + B158DRAFT_1922 0.32 +0.9 58,320 - B158DRAFT_1922 0.32 +1.3 58,335 - B158DRAFT_1922 0.33 -1.1 58,335 - B158DRAFT_1922 0.33 -0.5 58,354 - B158DRAFT_1922 0.34 -0.8 58,359 - B158DRAFT_1922 0.34 +1.6
Or see this region's nucleotide sequence