Experiment: m.b. Dimethyl Sulfoxide 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1918 and B158DRAFT_1919 are separated by 15 nucleotides B158DRAFT_1919 and B158DRAFT_1920 are separated by 12 nucleotides B158DRAFT_1920 and B158DRAFT_1921 are separated by 251 nucleotides
B158DRAFT_1918: B158DRAFT_1918 - 1-deoxy-D-xylulose-5-phosphate synthase (EC 2.2.1.7), at 52,460 to 54,298
_1918
B158DRAFT_1919: B158DRAFT_1919 - farnesyl-diphosphate synthase (EC 2.5.1.10), at 54,314 to 55,204
_1919
B158DRAFT_1920: B158DRAFT_1920 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 55,217 to 55,462
_1920
B158DRAFT_1921: B158DRAFT_1921 - glutaminyl-tRNA synthetase (EC 6.1.1.18), at 55,714 to 57,426
_1921
Position (kb) 55
56 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.216 kb on - strand at 55.264 kb on - strand, within B158DRAFT_1920 at 55.323 kb on - strand, within B158DRAFT_1920 at 55.344 kb on - strand, within B158DRAFT_1920 at 55.376 kb on - strand, within B158DRAFT_1920 at 55.511 kb on - strand at 55.511 kb on - strand at 55.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 2.5 vol% remove 55,216 - +0.6 55,216 - -0.9 55,216 - -2.0 55,216 - -0.8 55,216 - +1.7 55,216 - +0.2 55,216 - +0.2 55,216 - -1.2 55,216 - -2.4 55,216 - +0.4 55,216 - -0.2 55,264 - B158DRAFT_1920 0.19 +3.8 55,323 - B158DRAFT_1920 0.43 +5.1 55,344 - B158DRAFT_1920 0.52 +5.2 55,376 - B158DRAFT_1920 0.65 +5.0 55,511 - +6.0 55,511 - +5.2 55,618 - -1.5
Or see this region's nucleotide sequence