Experiment: m.b. Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0978 and B158DRAFT_0979 are separated by 27 nucleotides B158DRAFT_0979 and B158DRAFT_0980 are separated by 74 nucleotides
B158DRAFT_0978: B158DRAFT_0978 - DNA topoisomerase IV, B subunit, proteobacterial, at 1,008,147 to 1,010,036
_0978
B158DRAFT_0979: B158DRAFT_0979 - DNA topoisomerase IV, A subunit, proteobacterial, at 1,010,064 to 1,012,304
_0979
B158DRAFT_0980: B158DRAFT_0980 - hypothetical protein, at 1,012,379 to 1,013,335
_0980
Position (kb)
1010
1011
1012
1013 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1012.280 kb on + strand at 1012.294 kb on - strand at 1012.298 kb on + strand at 1012.306 kb on - strand at 1012.306 kb on - strand at 1012.306 kb on - strand at 1012.306 kb on - strand at 1012.408 kb on + strand at 1012.408 kb on + strand at 1012.455 kb on + strand at 1012.455 kb on + strand at 1012.455 kb on + strand at 1012.455 kb on + strand at 1012.455 kb on + strand at 1012.463 kb on - strand at 1012.503 kb on + strand, within B158DRAFT_0980 at 1012.503 kb on + strand, within B158DRAFT_0980 at 1012.511 kb on + strand, within B158DRAFT_0980 at 1012.534 kb on + strand, within B158DRAFT_0980 at 1012.542 kb on - strand, within B158DRAFT_0980 at 1012.627 kb on + strand, within B158DRAFT_0980 at 1012.644 kb on + strand, within B158DRAFT_0980 at 1012.644 kb on + strand, within B158DRAFT_0980 at 1012.644 kb on + strand, within B158DRAFT_0980 at 1012.644 kb on + strand, within B158DRAFT_0980 at 1012.644 kb on + strand, within B158DRAFT_0980 at 1012.652 kb on - strand, within B158DRAFT_0980 at 1012.652 kb on - strand, within B158DRAFT_0980 at 1012.652 kb on - strand, within B158DRAFT_0980 at 1012.652 kb on - strand, within B158DRAFT_0980 at 1012.791 kb on + strand, within B158DRAFT_0980 at 1012.801 kb on + strand, within B158DRAFT_0980 at 1012.801 kb on + strand, within B158DRAFT_0980 at 1012.809 kb on - strand, within B158DRAFT_0980 at 1012.809 kb on - strand, within B158DRAFT_0980 at 1012.838 kb on + strand, within B158DRAFT_0980 at 1012.838 kb on + strand, within B158DRAFT_0980 at 1012.877 kb on + strand, within B158DRAFT_0980 at 1012.909 kb on + strand, within B158DRAFT_0980 at 1013.008 kb on + strand, within B158DRAFT_0980 at 1013.069 kb on + strand, within B158DRAFT_0980 at 1013.083 kb on + strand, within B158DRAFT_0980 at 1013.093 kb on + strand, within B158DRAFT_0980 at 1013.153 kb on + strand, within B158DRAFT_0980 at 1013.158 kb on + strand, within B158DRAFT_0980 at 1013.166 kb on - strand, within B158DRAFT_0980 at 1013.166 kb on - strand, within B158DRAFT_0980 at 1013.166 kb on - strand, within B158DRAFT_0980 at 1013.166 kb on - strand, within B158DRAFT_0980 at 1013.166 kb on - strand, within B158DRAFT_0980 at 1013.178 kb on - strand, within B158DRAFT_0980 at 1013.202 kb on - strand, within B158DRAFT_0980 at 1013.221 kb on - strand, within B158DRAFT_0980 at 1013.277 kb on + strand at 1013.285 kb on - strand at 1013.290 kb on - strand at 1013.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 5 vol% remove 1,012,280 + -1.6 1,012,294 - -0.6 1,012,298 + -2.1 1,012,306 - +1.6 1,012,306 - +0.6 1,012,306 - -0.8 1,012,306 - -0.4 1,012,408 + +2.0 1,012,408 + +1.3 1,012,455 + +2.5 1,012,455 + +1.2 1,012,455 + +1.3 1,012,455 + -3.0 1,012,455 + +0.3 1,012,463 - +1.2 1,012,503 + B158DRAFT_0980 0.13 +1.9 1,012,503 + B158DRAFT_0980 0.13 +0.5 1,012,511 + B158DRAFT_0980 0.14 +1.5 1,012,534 + B158DRAFT_0980 0.16 +1.8 1,012,542 - B158DRAFT_0980 0.17 +1.4 1,012,627 + B158DRAFT_0980 0.26 +2.6 1,012,644 + B158DRAFT_0980 0.28 +1.1 1,012,644 + B158DRAFT_0980 0.28 +1.4 1,012,644 + B158DRAFT_0980 0.28 +1.5 1,012,644 + B158DRAFT_0980 0.28 +1.8 1,012,644 + B158DRAFT_0980 0.28 +1.8 1,012,652 - B158DRAFT_0980 0.29 +1.7 1,012,652 - B158DRAFT_0980 0.29 +1.2 1,012,652 - B158DRAFT_0980 0.29 +1.7 1,012,652 - B158DRAFT_0980 0.29 +0.6 1,012,791 + B158DRAFT_0980 0.43 +1.7 1,012,801 + B158DRAFT_0980 0.44 +0.6 1,012,801 + B158DRAFT_0980 0.44 +0.8 1,012,809 - B158DRAFT_0980 0.45 +1.6 1,012,809 - B158DRAFT_0980 0.45 +1.6 1,012,838 + B158DRAFT_0980 0.48 +1.6 1,012,838 + B158DRAFT_0980 0.48 +1.3 1,012,877 + B158DRAFT_0980 0.52 +2.7 1,012,909 + B158DRAFT_0980 0.55 +1.1 1,013,008 + B158DRAFT_0980 0.66 +1.9 1,013,069 + B158DRAFT_0980 0.72 +1.2 1,013,083 + B158DRAFT_0980 0.74 +1.3 1,013,093 + B158DRAFT_0980 0.75 +1.4 1,013,153 + B158DRAFT_0980 0.81 +3.4 1,013,158 + B158DRAFT_0980 0.81 +1.9 1,013,166 - B158DRAFT_0980 0.82 +1.0 1,013,166 - B158DRAFT_0980 0.82 +1.5 1,013,166 - B158DRAFT_0980 0.82 +2.3 1,013,166 - B158DRAFT_0980 0.82 +2.0 1,013,166 - B158DRAFT_0980 0.82 +2.2 1,013,178 - B158DRAFT_0980 0.83 +2.4 1,013,202 - B158DRAFT_0980 0.86 +2.3 1,013,221 - B158DRAFT_0980 0.88 +2.6 1,013,277 + +2.2 1,013,285 - +1.9 1,013,290 - +1.7 1,013,290 - +2.5
Or see this region's nucleotide sequence