Experiment: m.b. Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2266 and B158DRAFT_2267 are separated by 97 nucleotides B158DRAFT_2267 and B158DRAFT_2268 are separated by 92 nucleotides B158DRAFT_2268 and B158DRAFT_2269 are separated by 29 nucleotides B158DRAFT_2269 and B158DRAFT_2270 are separated by 191 nucleotides
B158DRAFT_2266: B158DRAFT_2266 - Protein-disulfide isomerase, at 428,734 to 429,492
_2266
B158DRAFT_2267: B158DRAFT_2267 - Uncharacterized conserved protein, at 429,590 to 429,958
_2267
B158DRAFT_2268: B158DRAFT_2268 - single-stranded-DNA-specific exonuclease RecJ, at 430,051 to 431,778
_2268
B158DRAFT_2269: B158DRAFT_2269 - hypothetical protein, at 431,808 to 432,227
_2269
B158DRAFT_2270: B158DRAFT_2270 - Small-conductance mechanosensitive channel, at 432,419 to 433,258
_2270
Position (kb)
430
431
432 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 429.070 kb on + strand, within B158DRAFT_2266 at 429.234 kb on - strand, within B158DRAFT_2266 at 429.252 kb on + strand, within B158DRAFT_2266 at 429.267 kb on - strand, within B158DRAFT_2266 at 429.352 kb on + strand, within B158DRAFT_2266 at 429.469 kb on - strand at 429.479 kb on - strand at 429.505 kb on - strand at 429.516 kb on - strand at 429.535 kb on + strand at 429.538 kb on - strand at 429.594 kb on + strand at 429.594 kb on + strand at 429.596 kb on + strand at 429.597 kb on - strand at 429.600 kb on + strand at 429.602 kb on - strand at 429.607 kb on - strand at 429.613 kb on - strand at 429.618 kb on - strand at 429.639 kb on + strand, within B158DRAFT_2267 at 429.663 kb on - strand, within B158DRAFT_2267 at 429.671 kb on - strand, within B158DRAFT_2267 at 429.700 kb on - strand, within B158DRAFT_2267 at 429.733 kb on + strand, within B158DRAFT_2267 at 429.784 kb on + strand, within B158DRAFT_2267 at 429.784 kb on - strand, within B158DRAFT_2267 at 429.834 kb on + strand, within B158DRAFT_2267 at 429.924 kb on - strand at 429.935 kb on + strand at 429.936 kb on + strand at 429.936 kb on + strand at 429.944 kb on - strand at 429.944 kb on - strand at 429.944 kb on - strand at 429.944 kb on - strand at 429.974 kb on + strand at 429.974 kb on + strand at 429.974 kb on + strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 430.099 kb on + strand at 430.103 kb on + strand at 430.133 kb on - strand at 430.133 kb on - strand at 430.133 kb on - strand at 430.142 kb on + strand at 430.142 kb on + strand at 430.142 kb on + strand at 430.150 kb on - strand at 430.150 kb on - strand at 430.150 kb on - strand at 430.159 kb on + strand at 430.219 kb on + strand at 430.389 kb on - strand, within B158DRAFT_2268 at 430.395 kb on - strand, within B158DRAFT_2268 at 430.422 kb on + strand, within B158DRAFT_2268 at 430.476 kb on + strand, within B158DRAFT_2268 at 430.476 kb on + strand, within B158DRAFT_2268 at 430.476 kb on + strand, within B158DRAFT_2268 at 430.484 kb on - strand, within B158DRAFT_2268 at 430.532 kb on - strand, within B158DRAFT_2268 at 430.671 kb on - strand, within B158DRAFT_2268 at 430.688 kb on + strand, within B158DRAFT_2268 at 430.688 kb on + strand, within B158DRAFT_2268 at 430.696 kb on - strand, within B158DRAFT_2268 at 430.696 kb on - strand, within B158DRAFT_2268 at 430.696 kb on - strand, within B158DRAFT_2268 at 430.757 kb on + strand, within B158DRAFT_2268 at 430.803 kb on + strand, within B158DRAFT_2268 at 430.811 kb on - strand, within B158DRAFT_2268 at 430.811 kb on - strand, within B158DRAFT_2268 at 430.813 kb on + strand, within B158DRAFT_2268 at 430.813 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.885 kb on + strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.893 kb on - strand, within B158DRAFT_2268 at 430.911 kb on + strand, within B158DRAFT_2268 at 430.919 kb on - strand, within B158DRAFT_2268 at 430.919 kb on - strand, within B158DRAFT_2268 at 430.936 kb on + strand, within B158DRAFT_2268 at 430.971 kb on + strand, within B158DRAFT_2268 at 430.971 kb on + strand, within B158DRAFT_2268 at 430.979 kb on - strand, within B158DRAFT_2268 at 431.021 kb on - strand, within B158DRAFT_2268 at 431.046 kb on - strand, within B158DRAFT_2268 at 431.052 kb on + strand, within B158DRAFT_2268 at 431.060 kb on - strand, within B158DRAFT_2268 at 431.091 kb on - strand, within B158DRAFT_2268 at 431.093 kb on + strand, within B158DRAFT_2268 at 431.195 kb on + strand, within B158DRAFT_2268 at 431.215 kb on + strand, within B158DRAFT_2268 at 431.215 kb on + strand, within B158DRAFT_2268 at 431.217 kb on + strand, within B158DRAFT_2268 at 431.223 kb on - strand, within B158DRAFT_2268 at 431.223 kb on - strand, within B158DRAFT_2268 at 431.223 kb on - strand, within B158DRAFT_2268 at 431.234 kb on - strand, within B158DRAFT_2268 at 431.241 kb on - strand, within B158DRAFT_2268 at 431.254 kb on + strand, within B158DRAFT_2268 at 431.255 kb on - strand, within B158DRAFT_2268 at 431.270 kb on - strand, within B158DRAFT_2268 at 431.349 kb on - strand, within B158DRAFT_2268 at 431.409 kb on + strand, within B158DRAFT_2268 at 431.409 kb on + strand, within B158DRAFT_2268 at 431.409 kb on + strand, within B158DRAFT_2268 at 431.409 kb on + strand, within B158DRAFT_2268 at 431.417 kb on - strand, within B158DRAFT_2268 at 431.417 kb on - strand, within B158DRAFT_2268 at 431.417 kb on - strand, within B158DRAFT_2268 at 431.420 kb on + strand, within B158DRAFT_2268 at 431.431 kb on - strand, within B158DRAFT_2268 at 431.454 kb on + strand, within B158DRAFT_2268 at 431.454 kb on + strand, within B158DRAFT_2268 at 431.459 kb on + strand, within B158DRAFT_2268 at 431.481 kb on - strand, within B158DRAFT_2268 at 431.481 kb on - strand, within B158DRAFT_2268 at 431.483 kb on + strand, within B158DRAFT_2268 at 431.483 kb on + strand, within B158DRAFT_2268 at 431.483 kb on + strand, within B158DRAFT_2268 at 431.483 kb on + strand, within B158DRAFT_2268 at 431.491 kb on - strand, within B158DRAFT_2268 at 431.491 kb on - strand, within B158DRAFT_2268 at 431.544 kb on - strand, within B158DRAFT_2268 at 431.609 kb on + strand at 431.617 kb on - strand at 431.634 kb on + strand at 431.642 kb on - strand at 431.642 kb on - strand at 431.656 kb on + strand at 431.688 kb on + strand at 431.688 kb on + strand at 431.696 kb on - strand at 431.696 kb on - strand at 431.731 kb on + strand at 431.767 kb on + strand at 431.775 kb on - strand at 431.798 kb on + strand at 431.798 kb on + strand at 431.798 kb on + strand at 431.798 kb on + strand at 431.806 kb on - strand at 431.843 kb on + strand at 431.849 kb on - strand at 431.888 kb on + strand, within B158DRAFT_2269 at 431.901 kb on - strand, within B158DRAFT_2269 at 431.903 kb on + strand, within B158DRAFT_2269 at 431.939 kb on + strand, within B158DRAFT_2269 at 431.992 kb on - strand, within B158DRAFT_2269 at 432.032 kb on - strand, within B158DRAFT_2269 at 432.034 kb on + strand, within B158DRAFT_2269 at 432.034 kb on + strand, within B158DRAFT_2269 at 432.034 kb on + strand, within B158DRAFT_2269 at 432.039 kb on + strand, within B158DRAFT_2269 at 432.042 kb on - strand, within B158DRAFT_2269 at 432.042 kb on - strand, within B158DRAFT_2269 at 432.047 kb on - strand, within B158DRAFT_2269 at 432.047 kb on - strand, within B158DRAFT_2269 at 432.076 kb on + strand, within B158DRAFT_2269 at 432.076 kb on + strand, within B158DRAFT_2269 at 432.084 kb on - strand, within B158DRAFT_2269 at 432.084 kb on - strand, within B158DRAFT_2269 at 432.084 kb on - strand, within B158DRAFT_2269 at 432.154 kb on - strand, within B158DRAFT_2269 at 432.182 kb on + strand, within B158DRAFT_2269 at 432.182 kb on + strand, within B158DRAFT_2269 at 432.190 kb on - strand at 432.190 kb on - strand at 432.191 kb on - strand at 432.233 kb on - strand at 432.647 kb on + strand, within B158DRAFT_2270 at 432.706 kb on + strand, within B158DRAFT_2270 at 432.706 kb on + strand, within B158DRAFT_2270 at 432.742 kb on + strand, within B158DRAFT_2270 at 432.742 kb on + strand, within B158DRAFT_2270 at 432.742 kb on + strand, within B158DRAFT_2270 at 432.750 kb on - strand, within B158DRAFT_2270 at 432.750 kb on - strand, within B158DRAFT_2270 at 432.750 kb on - strand, within B158DRAFT_2270 at 432.750 kb on - strand, within B158DRAFT_2270 at 432.752 kb on + strand, within B158DRAFT_2270
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloride 500 mM remove 429,070 + B158DRAFT_2266 0.44 +0.6 429,234 - B158DRAFT_2266 0.66 +0.8 429,252 + B158DRAFT_2266 0.68 +3.0 429,267 - B158DRAFT_2266 0.70 +1.7 429,352 + B158DRAFT_2266 0.81 -2.2 429,469 - +0.7 429,479 - +1.2 429,505 - -0.7 429,516 - -0.4 429,535 + +0.6 429,538 - -0.7 429,594 + +2.7 429,594 + +1.8 429,596 + +1.7 429,597 - -2.1 429,600 + +2.2 429,602 - -1.1 429,607 - +1.9 429,613 - +2.5 429,618 - +2.4 429,639 + B158DRAFT_2267 0.13 +2.4 429,663 - B158DRAFT_2267 0.20 +1.8 429,671 - B158DRAFT_2267 0.22 +2.0 429,700 - B158DRAFT_2267 0.30 +2.8 429,733 + B158DRAFT_2267 0.39 +2.1 429,784 + B158DRAFT_2267 0.53 +2.1 429,784 - B158DRAFT_2267 0.53 +2.4 429,834 + B158DRAFT_2267 0.66 +2.3 429,924 - +2.6 429,935 + +2.4 429,936 + +2.5 429,936 + +1.9 429,944 - +1.1 429,944 - -1.3 429,944 - +0.4 429,944 - +1.3 429,974 + +2.5 429,974 + +2.7 429,974 + +2.5 429,982 - -1.1 429,982 - +1.4 429,982 - +1.9 429,982 - +0.7 429,982 - -0.6 429,982 - +1.0 430,099 + +1.3 430,103 + +1.0 430,133 - +1.0 430,133 - +1.3 430,133 - -0.4 430,142 + +1.4 430,142 + +0.9 430,142 + +1.6 430,150 - +1.5 430,150 - +1.6 430,150 - +1.5 430,159 + +3.9 430,219 + +1.8 430,389 - B158DRAFT_2268 0.20 +2.1 430,395 - B158DRAFT_2268 0.20 +0.0 430,422 + B158DRAFT_2268 0.21 +0.4 430,476 + B158DRAFT_2268 0.25 +1.2 430,476 + B158DRAFT_2268 0.25 +2.7 430,476 + B158DRAFT_2268 0.25 +1.3 430,484 - B158DRAFT_2268 0.25 +0.4 430,532 - B158DRAFT_2268 0.28 +1.2 430,671 - B158DRAFT_2268 0.36 +0.5 430,688 + B158DRAFT_2268 0.37 +0.3 430,688 + B158DRAFT_2268 0.37 +1.3 430,696 - B158DRAFT_2268 0.37 +1.0 430,696 - B158DRAFT_2268 0.37 -0.4 430,696 - B158DRAFT_2268 0.37 +1.1 430,757 + B158DRAFT_2268 0.41 +1.7 430,803 + B158DRAFT_2268 0.44 -0.2 430,811 - B158DRAFT_2268 0.44 +1.6 430,811 - B158DRAFT_2268 0.44 +0.5 430,813 + B158DRAFT_2268 0.44 +1.8 430,813 + B158DRAFT_2268 0.44 +1.8 430,885 + B158DRAFT_2268 0.48 +1.4 430,885 + B158DRAFT_2268 0.48 +0.7 430,885 + B158DRAFT_2268 0.48 -0.5 430,885 + B158DRAFT_2268 0.48 +1.4 430,885 + B158DRAFT_2268 0.48 -0.7 430,885 + B158DRAFT_2268 0.48 +0.8 430,885 + B158DRAFT_2268 0.48 +1.1 430,885 + B158DRAFT_2268 0.48 +1.1 430,885 + B158DRAFT_2268 0.48 +0.4 430,893 - B158DRAFT_2268 0.49 +2.6 430,893 - B158DRAFT_2268 0.49 +1.0 430,893 - B158DRAFT_2268 0.49 +2.2 430,893 - B158DRAFT_2268 0.49 +2.6 430,893 - B158DRAFT_2268 0.49 +2.5 430,911 + B158DRAFT_2268 0.50 +2.1 430,919 - B158DRAFT_2268 0.50 +2.1 430,919 - B158DRAFT_2268 0.50 +1.5 430,936 + B158DRAFT_2268 0.51 +1.9 430,971 + B158DRAFT_2268 0.53 +0.6 430,971 + B158DRAFT_2268 0.53 -2.4 430,979 - B158DRAFT_2268 0.54 -0.3 431,021 - B158DRAFT_2268 0.56 +2.2 431,046 - B158DRAFT_2268 0.58 +1.2 431,052 + B158DRAFT_2268 0.58 +2.0 431,060 - B158DRAFT_2268 0.58 -1.0 431,091 - B158DRAFT_2268 0.60 +2.0 431,093 + B158DRAFT_2268 0.60 -0.9 431,195 + B158DRAFT_2268 0.66 +1.2 431,215 + B158DRAFT_2268 0.67 +1.4 431,215 + B158DRAFT_2268 0.67 +1.7 431,217 + B158DRAFT_2268 0.67 +0.9 431,223 - B158DRAFT_2268 0.68 +1.2 431,223 - B158DRAFT_2268 0.68 +1.8 431,223 - B158DRAFT_2268 0.68 +0.6 431,234 - B158DRAFT_2268 0.68 -0.5 431,241 - B158DRAFT_2268 0.69 +2.2 431,254 + B158DRAFT_2268 0.70 -0.6 431,255 - B158DRAFT_2268 0.70 -1.1 431,270 - B158DRAFT_2268 0.71 +0.6 431,349 - B158DRAFT_2268 0.75 +0.5 431,409 + B158DRAFT_2268 0.79 +1.8 431,409 + B158DRAFT_2268 0.79 +1.5 431,409 + B158DRAFT_2268 0.79 +1.9 431,409 + B158DRAFT_2268 0.79 +1.4 431,417 - B158DRAFT_2268 0.79 +0.6 431,417 - B158DRAFT_2268 0.79 +1.3 431,417 - B158DRAFT_2268 0.79 +1.5 431,420 + B158DRAFT_2268 0.79 +1.8 431,431 - B158DRAFT_2268 0.80 +1.9 431,454 + B158DRAFT_2268 0.81 -0.0 431,454 + B158DRAFT_2268 0.81 -0.1 431,459 + B158DRAFT_2268 0.81 +1.1 431,481 - B158DRAFT_2268 0.83 +1.8 431,481 - B158DRAFT_2268 0.83 +2.1 431,483 + B158DRAFT_2268 0.83 +2.6 431,483 + B158DRAFT_2268 0.83 +2.2 431,483 + B158DRAFT_2268 0.83 -0.0 431,483 + B158DRAFT_2268 0.83 +0.7 431,491 - B158DRAFT_2268 0.83 +1.6 431,491 - B158DRAFT_2268 0.83 +1.3 431,544 - B158DRAFT_2268 0.86 +0.6 431,609 + +0.8 431,617 - +0.2 431,634 + +1.4 431,642 - +2.5 431,642 - +0.9 431,656 + +0.9 431,688 + +1.4 431,688 + -0.4 431,696 - +0.6 431,696 - +1.7 431,731 + +2.3 431,767 + +0.6 431,775 - -0.5 431,798 + -1.0 431,798 + +0.7 431,798 + -1.0 431,798 + -0.7 431,806 - -0.5 431,843 + -0.0 431,849 - +0.1 431,888 + B158DRAFT_2269 0.19 -0.1 431,901 - B158DRAFT_2269 0.22 -0.9 431,903 + B158DRAFT_2269 0.23 +0.4 431,939 + B158DRAFT_2269 0.31 -1.5 431,992 - B158DRAFT_2269 0.44 -0.8 432,032 - B158DRAFT_2269 0.53 -2.2 432,034 + B158DRAFT_2269 0.54 +0.6 432,034 + B158DRAFT_2269 0.54 -0.0 432,034 + B158DRAFT_2269 0.54 -1.2 432,039 + B158DRAFT_2269 0.55 +0.3 432,042 - B158DRAFT_2269 0.56 -1.4 432,042 - B158DRAFT_2269 0.56 +0.5 432,047 - B158DRAFT_2269 0.57 -1.5 432,047 - B158DRAFT_2269 0.57 -0.8 432,076 + B158DRAFT_2269 0.64 -0.6 432,076 + B158DRAFT_2269 0.64 +0.6 432,084 - B158DRAFT_2269 0.66 -0.3 432,084 - B158DRAFT_2269 0.66 -0.6 432,084 - B158DRAFT_2269 0.66 -0.7 432,154 - B158DRAFT_2269 0.82 -0.7 432,182 + B158DRAFT_2269 0.89 +1.7 432,182 + B158DRAFT_2269 0.89 -0.4 432,190 - -0.9 432,190 - -1.0 432,191 - +0.0 432,233 - -1.4 432,647 + B158DRAFT_2270 0.27 -0.4 432,706 + B158DRAFT_2270 0.34 -0.2 432,706 + B158DRAFT_2270 0.34 +1.9 432,742 + B158DRAFT_2270 0.38 -0.6 432,742 + B158DRAFT_2270 0.38 -1.0 432,742 + B158DRAFT_2270 0.38 -1.2 432,750 - B158DRAFT_2270 0.39 +1.2 432,750 - B158DRAFT_2270 0.39 -0.8 432,750 - B158DRAFT_2270 0.39 -0.0 432,750 - B158DRAFT_2270 0.39 -1.1 432,752 + B158DRAFT_2270 0.40 -0.5
Or see this region's nucleotide sequence