Experiment: m.b. Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1446 and B158DRAFT_1447 are separated by 1 nucleotides B158DRAFT_1447 and B158DRAFT_1448 are separated by 48 nucleotides
B158DRAFT_1446: B158DRAFT_1446 - 4-hydroxyphenylpyruvate dioxygenase, at 1,499,982 to 1,501,043
_1446
B158DRAFT_1447: B158DRAFT_1447 - homogentisate 1,2-dioxygenase (EC 1.13.11.5), at 1,501,045 to 1,502,175
_1447
B158DRAFT_1448: B158DRAFT_1448 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 1,502,224 to 1,503,213
_1448
Position (kb)
1501
1502
1503 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1500.177 kb on - strand, within B158DRAFT_1446 at 1500.187 kb on + strand, within B158DRAFT_1446 at 1500.224 kb on - strand, within B158DRAFT_1446 at 1500.226 kb on + strand, within B158DRAFT_1446 at 1500.233 kb on + strand, within B158DRAFT_1446 at 1500.276 kb on + strand, within B158DRAFT_1446 at 1500.284 kb on - strand, within B158DRAFT_1446 at 1500.284 kb on - strand, within B158DRAFT_1446 at 1500.286 kb on + strand, within B158DRAFT_1446 at 1500.286 kb on + strand, within B158DRAFT_1446 at 1500.294 kb on - strand, within B158DRAFT_1446 at 1500.323 kb on - strand, within B158DRAFT_1446 at 1500.351 kb on + strand, within B158DRAFT_1446 at 1500.351 kb on + strand, within B158DRAFT_1446 at 1500.351 kb on + strand, within B158DRAFT_1446 at 1500.351 kb on + strand, within B158DRAFT_1446 at 1500.351 kb on + strand, within B158DRAFT_1446 at 1500.354 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.359 kb on - strand, within B158DRAFT_1446 at 1500.392 kb on - strand, within B158DRAFT_1446 at 1500.445 kb on + strand, within B158DRAFT_1446 at 1500.460 kb on + strand, within B158DRAFT_1446 at 1500.460 kb on + strand, within B158DRAFT_1446 at 1500.460 kb on - strand, within B158DRAFT_1446 at 1500.481 kb on + strand, within B158DRAFT_1446 at 1500.488 kb on + strand, within B158DRAFT_1446 at 1500.496 kb on + strand, within B158DRAFT_1446 at 1500.496 kb on + strand, within B158DRAFT_1446 at 1500.496 kb on - strand, within B158DRAFT_1446 at 1500.504 kb on - strand, within B158DRAFT_1446 at 1500.579 kb on + strand, within B158DRAFT_1446 at 1500.579 kb on + strand, within B158DRAFT_1446 at 1500.579 kb on + strand at 1500.579 kb on + strand, within B158DRAFT_1446 at 1500.587 kb on - strand, within B158DRAFT_1446 at 1500.587 kb on - strand, within B158DRAFT_1446 at 1500.587 kb on - strand, within B158DRAFT_1446 at 1500.593 kb on + strand, within B158DRAFT_1446 at 1500.680 kb on + strand, within B158DRAFT_1446 at 1500.680 kb on + strand, within B158DRAFT_1446 at 1500.686 kb on - strand, within B158DRAFT_1446 at 1500.687 kb on + strand, within B158DRAFT_1446 at 1500.688 kb on - strand, within B158DRAFT_1446 at 1500.688 kb on - strand, within B158DRAFT_1446 at 1500.688 kb on - strand, within B158DRAFT_1446 at 1500.688 kb on - strand, within B158DRAFT_1446 at 1500.696 kb on + strand, within B158DRAFT_1446 at 1500.696 kb on + strand, within B158DRAFT_1446 at 1500.702 kb on - strand, within B158DRAFT_1446 at 1500.704 kb on - strand, within B158DRAFT_1446 at 1500.778 kb on + strand, within B158DRAFT_1446 at 1500.824 kb on + strand, within B158DRAFT_1446 at 1500.881 kb on - strand, within B158DRAFT_1446 at 1500.931 kb on - strand, within B158DRAFT_1446 at 1500.953 kb on + strand at 1500.961 kb on - strand at 1500.961 kb on - strand at 1500.971 kb on - strand at 1500.994 kb on - strand at 1500.994 kb on - strand at 1501.006 kb on - strand at 1501.023 kb on + strand at 1501.037 kb on + strand at 1501.090 kb on + strand at 1501.090 kb on + strand at 1501.090 kb on + strand at 1501.090 kb on + strand at 1501.090 kb on + strand at 1501.095 kb on + strand at 1501.098 kb on - strand at 1501.108 kb on - strand at 1501.139 kb on - strand at 1501.155 kb on + strand at 1501.260 kb on + strand, within B158DRAFT_1447 at 1501.260 kb on + strand, within B158DRAFT_1447 at 1501.260 kb on + strand, within B158DRAFT_1447 at 1501.260 kb on + strand, within B158DRAFT_1447 at 1501.260 kb on + strand, within B158DRAFT_1447 at 1501.260 kb on + strand, within B158DRAFT_1447 at 1501.260 kb on + strand, within B158DRAFT_1447 at 1501.260 kb on + strand, within B158DRAFT_1447 at 1501.268 kb on - strand, within B158DRAFT_1447 at 1501.268 kb on - strand, within B158DRAFT_1447 at 1501.268 kb on - strand, within B158DRAFT_1447 at 1501.268 kb on - strand, within B158DRAFT_1447 at 1501.268 kb on - strand, within B158DRAFT_1447 at 1501.268 kb on - strand, within B158DRAFT_1447 at 1501.278 kb on - strand, within B158DRAFT_1447 at 1501.283 kb on + strand, within B158DRAFT_1447 at 1501.291 kb on + strand, within B158DRAFT_1447 at 1501.291 kb on - strand, within B158DRAFT_1447 at 1501.354 kb on - strand, within B158DRAFT_1447 at 1501.395 kb on + strand, within B158DRAFT_1447 at 1501.395 kb on + strand, within B158DRAFT_1447 at 1501.395 kb on + strand, within B158DRAFT_1447 at 1501.395 kb on + strand, within B158DRAFT_1447 at 1501.403 kb on - strand, within B158DRAFT_1447 at 1501.403 kb on - strand, within B158DRAFT_1447 at 1501.403 kb on - strand, within B158DRAFT_1447 at 1501.403 kb on - strand, within B158DRAFT_1447 at 1501.403 kb on - strand, within B158DRAFT_1447 at 1501.403 kb on - strand, within B158DRAFT_1447 at 1501.403 kb on - strand, within B158DRAFT_1447 at 1501.405 kb on + strand, within B158DRAFT_1447 at 1501.413 kb on - strand, within B158DRAFT_1447 at 1501.469 kb on + strand, within B158DRAFT_1447 at 1501.609 kb on - strand, within B158DRAFT_1447 at 1501.615 kb on + strand, within B158DRAFT_1447 at 1501.651 kb on - strand, within B158DRAFT_1447 at 1501.801 kb on + strand, within B158DRAFT_1447 at 1501.801 kb on + strand, within B158DRAFT_1447 at 1501.810 kb on + strand, within B158DRAFT_1447 at 1501.876 kb on - strand, within B158DRAFT_1447 at 1501.881 kb on - strand, within B158DRAFT_1447 at 1501.924 kb on - strand, within B158DRAFT_1447 at 1501.986 kb on - strand, within B158DRAFT_1447 at 1502.002 kb on + strand, within B158DRAFT_1447 at 1502.009 kb on + strand, within B158DRAFT_1447 at 1502.030 kb on - strand, within B158DRAFT_1447 at 1502.039 kb on - strand, within B158DRAFT_1447 at 1502.041 kb on + strand, within B158DRAFT_1447 at 1502.041 kb on + strand, within B158DRAFT_1447 at 1502.109 kb on - strand at 1502.110 kb on - strand at 1502.129 kb on + strand at 1502.146 kb on - strand at 1502.153 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chloride 500 mM remove 1,500,177 - B158DRAFT_1446 0.18 +1.2 1,500,187 + B158DRAFT_1446 0.19 +1.0 1,500,224 - B158DRAFT_1446 0.23 +0.2 1,500,226 + B158DRAFT_1446 0.23 -1.0 1,500,233 + B158DRAFT_1446 0.24 -0.3 1,500,276 + B158DRAFT_1446 0.28 -1.3 1,500,284 - B158DRAFT_1446 0.28 -1.5 1,500,284 - B158DRAFT_1446 0.28 -0.1 1,500,286 + B158DRAFT_1446 0.29 -0.7 1,500,286 + B158DRAFT_1446 0.29 -0.3 1,500,294 - B158DRAFT_1446 0.29 -1.7 1,500,323 - B158DRAFT_1446 0.32 -0.4 1,500,351 + B158DRAFT_1446 0.35 +0.3 1,500,351 + B158DRAFT_1446 0.35 -1.3 1,500,351 + B158DRAFT_1446 0.35 -0.0 1,500,351 + B158DRAFT_1446 0.35 -2.9 1,500,351 + B158DRAFT_1446 0.35 -0.7 1,500,354 - B158DRAFT_1446 0.35 +1.6 1,500,359 - B158DRAFT_1446 0.35 -2.7 1,500,359 - B158DRAFT_1446 0.35 -0.4 1,500,359 - B158DRAFT_1446 0.35 -1.4 1,500,359 - B158DRAFT_1446 0.35 -1.0 1,500,359 - B158DRAFT_1446 0.35 -1.1 1,500,359 - B158DRAFT_1446 0.35 -1.2 1,500,359 - B158DRAFT_1446 0.35 -0.1 1,500,359 - B158DRAFT_1446 0.35 +0.1 1,500,359 - B158DRAFT_1446 0.35 -0.5 1,500,359 - B158DRAFT_1446 0.35 -0.2 1,500,359 - B158DRAFT_1446 0.35 -0.8 1,500,359 - B158DRAFT_1446 0.35 -0.9 1,500,359 - B158DRAFT_1446 0.35 -1.3 1,500,392 - B158DRAFT_1446 0.39 -0.4 1,500,445 + B158DRAFT_1446 0.44 -1.0 1,500,460 + B158DRAFT_1446 0.45 -1.4 1,500,460 + B158DRAFT_1446 0.45 -0.9 1,500,460 - B158DRAFT_1446 0.45 -0.9 1,500,481 + B158DRAFT_1446 0.47 -1.3 1,500,488 + B158DRAFT_1446 0.48 -0.1 1,500,496 + B158DRAFT_1446 0.48 -1.7 1,500,496 + B158DRAFT_1446 0.48 +0.5 1,500,496 - B158DRAFT_1446 0.48 -0.2 1,500,504 - B158DRAFT_1446 0.49 -0.4 1,500,579 + B158DRAFT_1446 0.56 -1.1 1,500,579 + B158DRAFT_1446 0.56 -0.5 1,500,579 + +2.0 1,500,579 + B158DRAFT_1446 0.56 +0.3 1,500,587 - B158DRAFT_1446 0.57 -0.9 1,500,587 - B158DRAFT_1446 0.57 -1.5 1,500,587 - B158DRAFT_1446 0.57 +0.0 1,500,593 + B158DRAFT_1446 0.58 +0.3 1,500,680 + B158DRAFT_1446 0.66 -0.2 1,500,680 + B158DRAFT_1446 0.66 -0.9 1,500,686 - B158DRAFT_1446 0.66 -0.8 1,500,687 + B158DRAFT_1446 0.66 -1.0 1,500,688 - B158DRAFT_1446 0.66 -2.7 1,500,688 - B158DRAFT_1446 0.66 -0.4 1,500,688 - B158DRAFT_1446 0.66 -0.7 1,500,688 - B158DRAFT_1446 0.66 -0.6 1,500,696 + B158DRAFT_1446 0.67 -0.3 1,500,696 + B158DRAFT_1446 0.67 -0.3 1,500,702 - B158DRAFT_1446 0.68 -2.4 1,500,704 - B158DRAFT_1446 0.68 -1.3 1,500,778 + B158DRAFT_1446 0.75 +0.4 1,500,824 + B158DRAFT_1446 0.79 -0.1 1,500,881 - B158DRAFT_1446 0.85 -1.9 1,500,931 - B158DRAFT_1446 0.89 -1.1 1,500,953 + -1.0 1,500,961 - -3.0 1,500,961 - +1.4 1,500,971 - -0.5 1,500,994 - -0.8 1,500,994 - -2.4 1,501,006 - +0.6 1,501,023 + -1.5 1,501,037 + -0.2 1,501,090 + -0.5 1,501,090 + +1.8 1,501,090 + +0.1 1,501,090 + +1.1 1,501,090 + +0.1 1,501,095 + +0.8 1,501,098 - +1.8 1,501,108 - -1.3 1,501,139 - -1.7 1,501,155 + +1.4 1,501,260 + B158DRAFT_1447 0.19 +0.3 1,501,260 + B158DRAFT_1447 0.19 +1.9 1,501,260 + B158DRAFT_1447 0.19 +2.5 1,501,260 + B158DRAFT_1447 0.19 +2.0 1,501,260 + B158DRAFT_1447 0.19 +2.3 1,501,260 + B158DRAFT_1447 0.19 +1.2 1,501,260 + B158DRAFT_1447 0.19 +0.3 1,501,260 + B158DRAFT_1447 0.19 +1.6 1,501,268 - B158DRAFT_1447 0.20 +0.6 1,501,268 - B158DRAFT_1447 0.20 +0.6 1,501,268 - B158DRAFT_1447 0.20 +0.7 1,501,268 - B158DRAFT_1447 0.20 +1.8 1,501,268 - B158DRAFT_1447 0.20 +1.2 1,501,268 - B158DRAFT_1447 0.20 +1.8 1,501,278 - B158DRAFT_1447 0.21 +1.7 1,501,283 + B158DRAFT_1447 0.21 +0.5 1,501,291 + B158DRAFT_1447 0.22 +1.9 1,501,291 - B158DRAFT_1447 0.22 +0.7 1,501,354 - B158DRAFT_1447 0.27 +0.6 1,501,395 + B158DRAFT_1447 0.31 +2.3 1,501,395 + B158DRAFT_1447 0.31 +2.0 1,501,395 + B158DRAFT_1447 0.31 +0.5 1,501,395 + B158DRAFT_1447 0.31 +2.5 1,501,403 - B158DRAFT_1447 0.32 +1.2 1,501,403 - B158DRAFT_1447 0.32 +1.2 1,501,403 - B158DRAFT_1447 0.32 +1.1 1,501,403 - B158DRAFT_1447 0.32 -0.9 1,501,403 - B158DRAFT_1447 0.32 +0.9 1,501,403 - B158DRAFT_1447 0.32 +1.6 1,501,403 - B158DRAFT_1447 0.32 +1.4 1,501,405 + B158DRAFT_1447 0.32 +1.1 1,501,413 - B158DRAFT_1447 0.33 +0.6 1,501,469 + B158DRAFT_1447 0.37 +1.8 1,501,609 - B158DRAFT_1447 0.50 +1.5 1,501,615 + B158DRAFT_1447 0.50 +1.7 1,501,651 - B158DRAFT_1447 0.54 -0.9 1,501,801 + B158DRAFT_1447 0.67 +1.0 1,501,801 + B158DRAFT_1447 0.67 +1.4 1,501,810 + B158DRAFT_1447 0.68 +2.8 1,501,876 - B158DRAFT_1447 0.73 +1.5 1,501,881 - B158DRAFT_1447 0.74 +1.8 1,501,924 - B158DRAFT_1447 0.78 -0.9 1,501,986 - B158DRAFT_1447 0.83 +1.8 1,502,002 + B158DRAFT_1447 0.85 +1.4 1,502,009 + B158DRAFT_1447 0.85 +1.7 1,502,030 - B158DRAFT_1447 0.87 +0.3 1,502,039 - B158DRAFT_1447 0.88 +1.3 1,502,041 + B158DRAFT_1447 0.88 +1.4 1,502,041 + B158DRAFT_1447 0.88 +2.0 1,502,109 - +1.1 1,502,110 - -0.3 1,502,129 + +1.4 1,502,146 - +0.9 1,502,153 - +2.0
Or see this region's nucleotide sequence