Experiment: m.b. Polymyxin B sulfate 0.0019531 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1492 and B158DRAFT_1493 are separated by 238 nucleotides B158DRAFT_1493 and B158DRAFT_1494 are separated by 220 nucleotides
B158DRAFT_1492: B158DRAFT_1492 - FAD/FMN-containing dehydrogenases, at 1,548,880 to 1,550,271
_1492
B158DRAFT_1493: B158DRAFT_1493 - tRNA_Val_TAC, at 1,550,510 to 1,550,585
_1493
B158DRAFT_1494: B158DRAFT_1494 - glutamyl-tRNA synthetase (EC 6.1.1.17), at 1,550,806 to 1,552,224
_1494
Position (kb)
1550
1551 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1549.536 kb on + strand, within B158DRAFT_1492 at 1549.536 kb on + strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.544 kb on - strand, within B158DRAFT_1492 at 1549.563 kb on - strand, within B158DRAFT_1492 at 1549.577 kb on - strand, within B158DRAFT_1492 at 1549.608 kb on - strand, within B158DRAFT_1492 at 1549.668 kb on + strand, within B158DRAFT_1492 at 1549.706 kb on - strand, within B158DRAFT_1492 at 1549.745 kb on - strand, within B158DRAFT_1492 at 1549.769 kb on + strand, within B158DRAFT_1492 at 1549.774 kb on + strand, within B158DRAFT_1492 at 1549.775 kb on - strand, within B158DRAFT_1492 at 1549.775 kb on - strand, within B158DRAFT_1492 at 1549.775 kb on - strand, within B158DRAFT_1492 at 1549.776 kb on + strand, within B158DRAFT_1492 at 1549.777 kb on - strand, within B158DRAFT_1492 at 1549.782 kb on - strand, within B158DRAFT_1492 at 1549.792 kb on + strand, within B158DRAFT_1492 at 1549.792 kb on + strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.800 kb on - strand, within B158DRAFT_1492 at 1549.854 kb on + strand, within B158DRAFT_1492 at 1549.854 kb on + strand, within B158DRAFT_1492 at 1549.857 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.862 kb on - strand, within B158DRAFT_1492 at 1549.871 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.883 kb on + strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.891 kb on - strand, within B158DRAFT_1492 at 1549.902 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.041 kb on + strand, within B158DRAFT_1492 at 1550.049 kb on - strand, within B158DRAFT_1492 at 1550.049 kb on - strand, within B158DRAFT_1492 at 1550.049 kb on - strand, within B158DRAFT_1492 at 1550.070 kb on - strand, within B158DRAFT_1492 at 1550.171 kb on + strand at 1550.171 kb on + strand at 1550.179 kb on - strand at 1550.179 kb on - strand at 1550.179 kb on - strand at 1550.179 kb on - strand at 1550.222 kb on - strand at 1550.325 kb on - strand at 1550.352 kb on + strand at 1550.395 kb on - strand at 1550.534 kb on - strand, within B158DRAFT_1493 at 1550.534 kb on - strand, within B158DRAFT_1493 at 1550.541 kb on - strand, within B158DRAFT_1493 at 1550.554 kb on + strand, within B158DRAFT_1493 at 1550.618 kb on + strand at 1550.626 kb on - strand at 1550.635 kb on - strand at 1550.640 kb on + strand at 1550.648 kb on - strand at 1550.688 kb on + strand at 1550.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0019531 mg/ml remove 1,549,536 + B158DRAFT_1492 0.47 -2.0 1,549,536 + B158DRAFT_1492 0.47 +1.2 1,549,544 - B158DRAFT_1492 0.48 -4.2 1,549,544 - B158DRAFT_1492 0.48 +1.0 1,549,544 - B158DRAFT_1492 0.48 -0.1 1,549,544 - B158DRAFT_1492 0.48 -2.4 1,549,544 - B158DRAFT_1492 0.48 -0.6 1,549,563 - B158DRAFT_1492 0.49 -0.0 1,549,577 - B158DRAFT_1492 0.50 -2.8 1,549,608 - B158DRAFT_1492 0.52 -3.8 1,549,668 + B158DRAFT_1492 0.57 -3.0 1,549,706 - B158DRAFT_1492 0.59 -3.8 1,549,745 - B158DRAFT_1492 0.62 -2.9 1,549,769 + B158DRAFT_1492 0.64 -0.4 1,549,774 + B158DRAFT_1492 0.64 -1.1 1,549,775 - B158DRAFT_1492 0.64 -4.0 1,549,775 - B158DRAFT_1492 0.64 +0.3 1,549,775 - B158DRAFT_1492 0.64 -0.5 1,549,776 + B158DRAFT_1492 0.64 -2.1 1,549,777 - B158DRAFT_1492 0.64 -0.6 1,549,782 - B158DRAFT_1492 0.65 +0.3 1,549,792 + B158DRAFT_1492 0.66 -1.4 1,549,792 + B158DRAFT_1492 0.66 +0.2 1,549,800 - B158DRAFT_1492 0.66 -3.5 1,549,800 - B158DRAFT_1492 0.66 -1.0 1,549,800 - B158DRAFT_1492 0.66 +0.4 1,549,800 - B158DRAFT_1492 0.66 +1.0 1,549,800 - B158DRAFT_1492 0.66 +2.5 1,549,800 - B158DRAFT_1492 0.66 -1.6 1,549,854 + B158DRAFT_1492 0.70 +0.1 1,549,854 + B158DRAFT_1492 0.70 -2.0 1,549,857 - B158DRAFT_1492 0.70 -3.0 1,549,862 - B158DRAFT_1492 0.71 +2.9 1,549,862 - B158DRAFT_1492 0.71 -3.1 1,549,862 - B158DRAFT_1492 0.71 -1.8 1,549,862 - B158DRAFT_1492 0.71 -4.0 1,549,862 - B158DRAFT_1492 0.71 -0.2 1,549,871 + B158DRAFT_1492 0.71 -0.8 1,549,883 + B158DRAFT_1492 0.72 -2.0 1,549,883 + B158DRAFT_1492 0.72 -2.5 1,549,883 + B158DRAFT_1492 0.72 -0.8 1,549,883 + B158DRAFT_1492 0.72 -2.0 1,549,883 + B158DRAFT_1492 0.72 -2.2 1,549,883 + B158DRAFT_1492 0.72 +0.2 1,549,891 - B158DRAFT_1492 0.73 -1.8 1,549,891 - B158DRAFT_1492 0.73 +1.4 1,549,891 - B158DRAFT_1492 0.73 +0.9 1,549,891 - B158DRAFT_1492 0.73 +1.8 1,549,891 - B158DRAFT_1492 0.73 -0.7 1,549,891 - B158DRAFT_1492 0.73 -0.4 1,549,902 + B158DRAFT_1492 0.73 -3.2 1,550,041 + B158DRAFT_1492 0.83 -2.5 1,550,041 + B158DRAFT_1492 0.83 -2.0 1,550,041 + B158DRAFT_1492 0.83 -1.4 1,550,041 + B158DRAFT_1492 0.83 -0.4 1,550,041 + B158DRAFT_1492 0.83 +1.7 1,550,041 + B158DRAFT_1492 0.83 -1.1 1,550,049 - B158DRAFT_1492 0.84 -3.9 1,550,049 - B158DRAFT_1492 0.84 -0.0 1,550,049 - B158DRAFT_1492 0.84 -0.3 1,550,070 - B158DRAFT_1492 0.85 -2.1 1,550,171 + -2.1 1,550,171 + -1.8 1,550,179 - -3.1 1,550,179 - +0.1 1,550,179 - +0.4 1,550,179 - -1.1 1,550,222 - -2.0 1,550,325 - -2.3 1,550,352 + +0.0 1,550,395 - +0.3 1,550,534 - B158DRAFT_1493 0.32 +0.1 1,550,534 - B158DRAFT_1493 0.32 +0.1 1,550,541 - B158DRAFT_1493 0.41 +0.9 1,550,554 + B158DRAFT_1493 0.58 +1.0 1,550,618 + -1.2 1,550,626 - -1.2 1,550,635 - -5.2 1,550,640 + -0.2 1,550,648 - +0.2 1,550,688 + -0.2 1,550,767 - -3.0
Or see this region's nucleotide sequence