Experiment: m.b. Polymyxin B sulfate 0.0019531 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0868 and B158DRAFT_0869 are separated by 111 nucleotides B158DRAFT_0869 and B158DRAFT_0870 overlap by 27 nucleotides B158DRAFT_0870 and B158DRAFT_0871 are separated by 94 nucleotides B158DRAFT_0871 and B158DRAFT_0872 are separated by 21 nucleotides
B158DRAFT_0868: B158DRAFT_0868 - hypothetical protein, at 885,889 to 886,593
_0868
B158DRAFT_0869: B158DRAFT_0869 - dethiobiotin synthase, at 886,705 to 887,400
_0869
B158DRAFT_0870: B158DRAFT_0870 - hypothetical protein, at 887,374 to 887,784
_0870
B158DRAFT_0871: B158DRAFT_0871 - Diacylglycerol kinase, at 887,879 to 888,250
_0871
B158DRAFT_0872: B158DRAFT_0872 - Predicted hydrolase of the alpha/beta-hydrolase fold, at 888,272 to 888,895
_0872
Position (kb)
887
888 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 886.404 kb on - strand, within B158DRAFT_0868 at 886.470 kb on - strand, within B158DRAFT_0868 at 886.507 kb on + strand, within B158DRAFT_0868 at 886.507 kb on + strand, within B158DRAFT_0868 at 886.507 kb on + strand, within B158DRAFT_0868 at 886.512 kb on + strand, within B158DRAFT_0868 at 886.512 kb on + strand, within B158DRAFT_0868 at 886.512 kb on + strand, within B158DRAFT_0868 at 886.515 kb on - strand, within B158DRAFT_0868 at 886.515 kb on - strand, within B158DRAFT_0868 at 886.515 kb on - strand, within B158DRAFT_0868 at 886.515 kb on - strand, within B158DRAFT_0868 at 886.567 kb on + strand at 886.575 kb on - strand at 887.359 kb on + strand at 887.381 kb on + strand at 887.381 kb on + strand at 887.397 kb on - strand at 887.436 kb on + strand, within B158DRAFT_0870 at 887.436 kb on + strand, within B158DRAFT_0870 at 887.530 kb on + strand, within B158DRAFT_0870 at 887.534 kb on - strand, within B158DRAFT_0870 at 887.555 kb on - strand, within B158DRAFT_0870 at 887.559 kb on + strand, within B158DRAFT_0870 at 887.559 kb on + strand, within B158DRAFT_0870 at 887.566 kb on + strand, within B158DRAFT_0870 at 887.566 kb on + strand, within B158DRAFT_0870 at 887.567 kb on - strand, within B158DRAFT_0870 at 887.574 kb on - strand, within B158DRAFT_0870 at 887.594 kb on - strand, within B158DRAFT_0870 at 887.597 kb on + strand, within B158DRAFT_0870 at 887.597 kb on + strand, within B158DRAFT_0870 at 887.597 kb on + strand, within B158DRAFT_0870 at 887.605 kb on - strand, within B158DRAFT_0870 at 887.607 kb on + strand, within B158DRAFT_0870 at 887.614 kb on + strand, within B158DRAFT_0870 at 887.622 kb on - strand, within B158DRAFT_0870 at 887.640 kb on - strand, within B158DRAFT_0870 at 887.699 kb on + strand, within B158DRAFT_0870 at 887.699 kb on + strand, within B158DRAFT_0870 at 887.707 kb on - strand, within B158DRAFT_0870 at 887.726 kb on - strand, within B158DRAFT_0870 at 887.728 kb on + strand, within B158DRAFT_0870 at 887.736 kb on - strand, within B158DRAFT_0870 at 887.736 kb on - strand, within B158DRAFT_0870 at 887.736 kb on - strand, within B158DRAFT_0870 at 887.741 kb on - strand, within B158DRAFT_0870 at 887.817 kb on + strand at 887.822 kb on + strand at 887.825 kb on - strand at 887.840 kb on + strand at 887.846 kb on - strand at 887.847 kb on - strand at 887.848 kb on - strand at 887.848 kb on - strand at 887.848 kb on - strand at 887.853 kb on - strand at 887.948 kb on - strand, within B158DRAFT_0871 at 887.963 kb on - strand, within B158DRAFT_0871 at 887.963 kb on - strand, within B158DRAFT_0871 at 887.963 kb on - strand, within B158DRAFT_0871 at 887.963 kb on - strand, within B158DRAFT_0871 at 888.002 kb on - strand, within B158DRAFT_0871 at 888.124 kb on + strand, within B158DRAFT_0871 at 888.155 kb on - strand, within B158DRAFT_0871 at 888.156 kb on - strand, within B158DRAFT_0871 at 888.184 kb on - strand, within B158DRAFT_0871 at 888.184 kb on - strand, within B158DRAFT_0871 at 888.199 kb on + strand, within B158DRAFT_0871 at 888.200 kb on - strand, within B158DRAFT_0871 at 888.207 kb on - strand, within B158DRAFT_0871 at 888.220 kb on - strand at 888.256 kb on - strand at 888.270 kb on + strand at 888.270 kb on + strand at 888.270 kb on + strand at 888.270 kb on + strand at 888.278 kb on - strand at 888.278 kb on - strand at 888.331 kb on - strand at 888.331 kb on - strand at 888.340 kb on + strand, within B158DRAFT_0872 at 888.340 kb on + strand, within B158DRAFT_0872 at 888.341 kb on - strand, within B158DRAFT_0872 at 888.348 kb on - strand, within B158DRAFT_0872 at 888.349 kb on + strand, within B158DRAFT_0872 at 888.349 kb on + strand, within B158DRAFT_0872 at 888.350 kb on + strand, within B158DRAFT_0872 at 888.363 kb on + strand, within B158DRAFT_0872 at 888.393 kb on + strand, within B158DRAFT_0872 at 888.393 kb on + strand, within B158DRAFT_0872 at 888.393 kb on + strand, within B158DRAFT_0872 at 888.393 kb on + strand, within B158DRAFT_0872 at 888.395 kb on - strand, within B158DRAFT_0872 at 888.401 kb on - strand, within B158DRAFT_0872 at 888.429 kb on + strand, within B158DRAFT_0872 at 888.429 kb on + strand, within B158DRAFT_0872 at 888.504 kb on + strand, within B158DRAFT_0872 at 888.523 kb on + strand, within B158DRAFT_0872 at 888.531 kb on + strand, within B158DRAFT_0872 at 888.540 kb on + strand, within B158DRAFT_0872 at 888.562 kb on - strand, within B158DRAFT_0872 at 888.562 kb on - strand, within B158DRAFT_0872 at 888.562 kb on - strand, within B158DRAFT_0872 at 888.680 kb on - strand, within B158DRAFT_0872 at 888.681 kb on + strand, within B158DRAFT_0872 at 888.703 kb on - strand, within B158DRAFT_0872 at 888.734 kb on - strand, within B158DRAFT_0872 at 888.776 kb on - strand, within B158DRAFT_0872
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0019531 mg/ml remove 886,404 - B158DRAFT_0868 0.73 -1.1 886,470 - B158DRAFT_0868 0.82 +0.1 886,507 + B158DRAFT_0868 0.88 -0.1 886,507 + B158DRAFT_0868 0.88 -3.6 886,507 + B158DRAFT_0868 0.88 +0.8 886,512 + B158DRAFT_0868 0.88 -4.4 886,512 + B158DRAFT_0868 0.88 -1.2 886,512 + B158DRAFT_0868 0.88 +0.1 886,515 - B158DRAFT_0868 0.89 -4.9 886,515 - B158DRAFT_0868 0.89 -3.5 886,515 - B158DRAFT_0868 0.89 +1.9 886,515 - B158DRAFT_0868 0.89 -1.6 886,567 + -1.3 886,575 - -0.7 887,359 + -0.2 887,381 + -0.2 887,381 + -0.1 887,397 - -1.2 887,436 + B158DRAFT_0870 0.15 +1.4 887,436 + B158DRAFT_0870 0.15 +0.8 887,530 + B158DRAFT_0870 0.38 -3.2 887,534 - B158DRAFT_0870 0.39 +0.6 887,555 - B158DRAFT_0870 0.44 -0.6 887,559 + B158DRAFT_0870 0.45 +0.6 887,559 + B158DRAFT_0870 0.45 -3.9 887,566 + B158DRAFT_0870 0.47 -0.3 887,566 + B158DRAFT_0870 0.47 +1.3 887,567 - B158DRAFT_0870 0.47 -0.0 887,574 - B158DRAFT_0870 0.49 -0.2 887,594 - B158DRAFT_0870 0.54 -1.8 887,597 + B158DRAFT_0870 0.54 -0.1 887,597 + B158DRAFT_0870 0.54 +0.1 887,597 + B158DRAFT_0870 0.54 -0.1 887,605 - B158DRAFT_0870 0.56 -0.0 887,607 + B158DRAFT_0870 0.57 +1.1 887,614 + B158DRAFT_0870 0.58 -0.3 887,622 - B158DRAFT_0870 0.60 +0.2 887,640 - B158DRAFT_0870 0.65 +0.1 887,699 + B158DRAFT_0870 0.79 -0.5 887,699 + B158DRAFT_0870 0.79 +0.0 887,707 - B158DRAFT_0870 0.81 -0.0 887,726 - B158DRAFT_0870 0.86 -0.0 887,728 + B158DRAFT_0870 0.86 +0.5 887,736 - B158DRAFT_0870 0.88 +0.2 887,736 - B158DRAFT_0870 0.88 +0.6 887,736 - B158DRAFT_0870 0.88 +0.3 887,741 - B158DRAFT_0870 0.89 -0.2 887,817 + +0.4 887,822 + -0.5 887,825 - -0.5 887,840 + +1.5 887,846 - -4.5 887,847 - +0.1 887,848 - -0.4 887,848 - -0.4 887,848 - +0.5 887,853 - -4.1 887,948 - B158DRAFT_0871 0.19 +1.2 887,963 - B158DRAFT_0871 0.23 -3.2 887,963 - B158DRAFT_0871 0.23 +0.8 887,963 - B158DRAFT_0871 0.23 -0.8 887,963 - B158DRAFT_0871 0.23 +0.3 888,002 - B158DRAFT_0871 0.33 -4.3 888,124 + B158DRAFT_0871 0.66 -0.1 888,155 - B158DRAFT_0871 0.74 +0.3 888,156 - B158DRAFT_0871 0.74 -0.2 888,184 - B158DRAFT_0871 0.82 -1.6 888,184 - B158DRAFT_0871 0.82 -1.1 888,199 + B158DRAFT_0871 0.86 +0.4 888,200 - B158DRAFT_0871 0.86 -3.6 888,207 - B158DRAFT_0871 0.88 -0.4 888,220 - -0.0 888,256 - -2.5 888,270 + -1.6 888,270 + +0.5 888,270 + -0.5 888,270 + -3.0 888,278 - -0.2 888,278 - -0.2 888,331 - -0.4 888,331 - +0.0 888,340 + B158DRAFT_0872 0.11 -2.6 888,340 + B158DRAFT_0872 0.11 +0.3 888,341 - B158DRAFT_0872 0.11 -0.2 888,348 - B158DRAFT_0872 0.12 -0.7 888,349 + B158DRAFT_0872 0.12 -3.1 888,349 + B158DRAFT_0872 0.12 -4.9 888,350 + B158DRAFT_0872 0.12 -0.0 888,363 + B158DRAFT_0872 0.15 -0.0 888,393 + B158DRAFT_0872 0.19 -1.3 888,393 + B158DRAFT_0872 0.19 -0.8 888,393 + B158DRAFT_0872 0.19 +1.4 888,393 + B158DRAFT_0872 0.19 +0.1 888,395 - B158DRAFT_0872 0.20 -0.7 888,401 - B158DRAFT_0872 0.21 +1.1 888,429 + B158DRAFT_0872 0.25 -0.8 888,429 + B158DRAFT_0872 0.25 +0.4 888,504 + B158DRAFT_0872 0.37 -0.1 888,523 + B158DRAFT_0872 0.40 -0.9 888,531 + B158DRAFT_0872 0.42 +0.9 888,540 + B158DRAFT_0872 0.43 -0.3 888,562 - B158DRAFT_0872 0.46 -0.6 888,562 - B158DRAFT_0872 0.46 +0.3 888,562 - B158DRAFT_0872 0.46 -0.4 888,680 - B158DRAFT_0872 0.65 +0.9 888,681 + B158DRAFT_0872 0.66 +0.6 888,703 - B158DRAFT_0872 0.69 -1.1 888,734 - B158DRAFT_0872 0.74 +0.7 888,776 - B158DRAFT_0872 0.81 -0.1
Or see this region's nucleotide sequence