Experiment: m.b. Polymyxin B sulfate 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2466 and B158DRAFT_2467 are separated by 456 nucleotides B158DRAFT_2467 and B158DRAFT_2468 are separated by 10 nucleotides B158DRAFT_2468 and B158DRAFT_2469 are separated by 11 nucleotides
B158DRAFT_2466: B158DRAFT_2466 - methionine synthase (B12-dependent) (EC 2.1.1.13), at 645,583 to 649,266
_2466
B158DRAFT_2467: B158DRAFT_2467 - Outer membrane cobalamin receptor protein, at 649,723 to 651,681
_2467
B158DRAFT_2468: B158DRAFT_2468 - ATP:cob(I)alamin adenosyltransferase, at 651,692 to 652,240
_2468
B158DRAFT_2469: B158DRAFT_2469 - hypothetical protein, at 652,252 to 652,779
_2469
Position (kb)
649
650
651
652 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 648.791 kb on + strand, within B158DRAFT_2466 at 648.799 kb on - strand, within B158DRAFT_2466 at 648.874 kb on + strand, within B158DRAFT_2466 at 648.874 kb on + strand, within B158DRAFT_2466 at 648.874 kb on + strand, within B158DRAFT_2466 at 648.874 kb on + strand, within B158DRAFT_2466 at 648.882 kb on - strand, within B158DRAFT_2466 at 648.882 kb on - strand, within B158DRAFT_2466 at 648.882 kb on - strand, within B158DRAFT_2466 at 648.882 kb on - strand, within B158DRAFT_2466 at 648.882 kb on - strand, within B158DRAFT_2466 at 648.882 kb on - strand, within B158DRAFT_2466 at 648.914 kb on + strand at 649.027 kb on + strand at 649.046 kb on - strand at 649.049 kb on + strand at 649.057 kb on - strand at 649.057 kb on - strand at 649.057 kb on - strand at 649.100 kb on + strand at 649.136 kb on - strand at 649.295 kb on + strand at 649.303 kb on - strand at 649.366 kb on - strand at 649.378 kb on - strand at 649.386 kb on - strand at 649.386 kb on - strand at 649.408 kb on + strand at 649.447 kb on + strand at 649.447 kb on + strand at 649.455 kb on - strand at 649.455 kb on - strand at 649.615 kb on + strand at 649.615 kb on + strand at 649.615 kb on + strand at 649.615 kb on + strand at 649.623 kb on - strand at 649.623 kb on - strand at 649.623 kb on - strand at 649.623 kb on - strand at 649.623 kb on - strand at 649.635 kb on - strand at 649.635 kb on - strand at 649.635 kb on - strand at 649.651 kb on + strand at 649.767 kb on - strand at 649.769 kb on + strand at 649.769 kb on + strand at 649.769 kb on + strand at 649.769 kb on + strand at 649.777 kb on - strand at 649.777 kb on - strand at 649.777 kb on - strand at 649.777 kb on - strand at 649.796 kb on + strand at 649.867 kb on + strand at 649.881 kb on + strand at 649.967 kb on - strand, within B158DRAFT_2467 at 650.160 kb on - strand, within B158DRAFT_2467 at 650.179 kb on + strand, within B158DRAFT_2467 at 650.234 kb on - strand, within B158DRAFT_2467 at 650.238 kb on + strand, within B158DRAFT_2467 at 650.246 kb on - strand, within B158DRAFT_2467 at 650.317 kb on - strand, within B158DRAFT_2467 at 650.490 kb on + strand, within B158DRAFT_2467 at 650.490 kb on + strand, within B158DRAFT_2467 at 650.495 kb on + strand, within B158DRAFT_2467 at 650.505 kb on + strand, within B158DRAFT_2467 at 650.505 kb on + strand, within B158DRAFT_2467 at 650.818 kb on - strand, within B158DRAFT_2467 at 650.902 kb on + strand, within B158DRAFT_2467 at 651.063 kb on + strand, within B158DRAFT_2467 at 651.071 kb on - strand, within B158DRAFT_2467 at 651.089 kb on + strand, within B158DRAFT_2467 at 651.097 kb on - strand, within B158DRAFT_2467 at 651.097 kb on - strand, within B158DRAFT_2467 at 651.159 kb on + strand, within B158DRAFT_2467 at 651.281 kb on - strand, within B158DRAFT_2467 at 651.325 kb on - strand, within B158DRAFT_2467 at 651.405 kb on - strand, within B158DRAFT_2467 at 651.407 kb on + strand, within B158DRAFT_2467 at 651.407 kb on + strand, within B158DRAFT_2467 at 651.514 kb on + strand at 651.627 kb on + strand at 651.627 kb on + strand at 651.627 kb on + strand at 651.635 kb on - strand at 651.635 kb on - strand at 651.712 kb on + strand at 651.712 kb on + strand at 651.712 kb on + strand at 651.717 kb on + strand at 651.717 kb on + strand at 651.717 kb on + strand at 651.720 kb on - strand at 651.722 kb on + strand at 651.725 kb on - strand at 651.725 kb on - strand at 651.725 kb on - strand at 651.725 kb on - strand at 651.725 kb on - strand at 651.725 kb on - strand at 651.745 kb on - strand at 651.812 kb on - strand, within B158DRAFT_2468 at 651.863 kb on + strand, within B158DRAFT_2468 at 651.871 kb on - strand, within B158DRAFT_2468 at 651.907 kb on + strand, within B158DRAFT_2468 at 651.923 kb on + strand, within B158DRAFT_2468 at 651.928 kb on + strand, within B158DRAFT_2468 at 651.928 kb on + strand, within B158DRAFT_2468 at 651.931 kb on - strand, within B158DRAFT_2468 at 651.933 kb on + strand, within B158DRAFT_2468 at 651.933 kb on + strand, within B158DRAFT_2468 at 651.933 kb on + strand, within B158DRAFT_2468 at 651.933 kb on + strand, within B158DRAFT_2468 at 651.941 kb on - strand, within B158DRAFT_2468 at 651.941 kb on - strand, within B158DRAFT_2468 at 651.941 kb on - strand, within B158DRAFT_2468 at 651.941 kb on - strand, within B158DRAFT_2468 at 651.941 kb on - strand, within B158DRAFT_2468 at 651.963 kb on - strand, within B158DRAFT_2468 at 651.965 kb on - strand, within B158DRAFT_2468 at 652.049 kb on - strand, within B158DRAFT_2468 at 652.075 kb on + strand, within B158DRAFT_2468 at 652.089 kb on + strand, within B158DRAFT_2468 at 652.141 kb on + strand, within B158DRAFT_2468 at 652.274 kb on + strand at 652.274 kb on + strand at 652.280 kb on + strand at 652.280 kb on + strand at 652.280 kb on + strand at 652.280 kb on + strand at 652.288 kb on - strand at 652.288 kb on - strand at 652.288 kb on - strand at 652.359 kb on + strand, within B158DRAFT_2469 at 652.452 kb on + strand, within B158DRAFT_2469 at 652.480 kb on + strand, within B158DRAFT_2469 at 652.495 kb on - strand, within B158DRAFT_2469 at 652.497 kb on + strand, within B158DRAFT_2469 at 652.497 kb on + strand, within B158DRAFT_2469 at 652.497 kb on + strand, within B158DRAFT_2469 at 652.497 kb on + strand, within B158DRAFT_2469 at 652.497 kb on + strand, within B158DRAFT_2469 at 652.505 kb on - strand, within B158DRAFT_2469 at 652.505 kb on - strand, within B158DRAFT_2469 at 652.505 kb on - strand, within B158DRAFT_2469 at 652.655 kb on - strand, within B158DRAFT_2469 at 652.660 kb on + strand, within B158DRAFT_2469 at 652.660 kb on + strand, within B158DRAFT_2469 at 652.660 kb on + strand, within B158DRAFT_2469 at 652.668 kb on - strand, within B158DRAFT_2469
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.00390625 mg/ml remove 648,791 + B158DRAFT_2466 0.87 -0.2 648,799 - B158DRAFT_2466 0.87 -0.5 648,874 + B158DRAFT_2466 0.89 +0.6 648,874 + B158DRAFT_2466 0.89 -0.7 648,874 + B158DRAFT_2466 0.89 -0.9 648,874 + B158DRAFT_2466 0.89 -3.3 648,882 - B158DRAFT_2466 0.90 +0.2 648,882 - B158DRAFT_2466 0.90 -0.5 648,882 - B158DRAFT_2466 0.90 -1.3 648,882 - B158DRAFT_2466 0.90 -0.1 648,882 - B158DRAFT_2466 0.90 -1.6 648,882 - B158DRAFT_2466 0.90 +0.3 648,914 + +0.5 649,027 + +0.2 649,046 - -1.0 649,049 + +0.3 649,057 - -2.5 649,057 - +0.2 649,057 - -1.1 649,100 + -0.0 649,136 - -0.0 649,295 + -0.2 649,303 - -0.9 649,366 - -1.8 649,378 - -0.4 649,386 - +1.5 649,386 - -0.3 649,408 + -0.3 649,447 + -0.9 649,447 + +2.7 649,455 - -3.4 649,455 - -0.5 649,615 + -0.2 649,615 + +0.3 649,615 + -0.6 649,615 + -0.2 649,623 - +0.2 649,623 - -1.4 649,623 - +0.1 649,623 - -0.6 649,623 - -3.2 649,635 - +0.3 649,635 - +0.2 649,635 - -0.6 649,651 + +0.6 649,767 - +0.2 649,769 + -0.5 649,769 + -0.0 649,769 + +0.4 649,769 + +0.5 649,777 - -0.5 649,777 - +0.0 649,777 - +0.8 649,777 - -0.1 649,796 + -3.9 649,867 + +0.3 649,881 + -4.5 649,967 - B158DRAFT_2467 0.12 -0.4 650,160 - B158DRAFT_2467 0.22 -4.6 650,179 + B158DRAFT_2467 0.23 +1.8 650,234 - B158DRAFT_2467 0.26 -1.2 650,238 + B158DRAFT_2467 0.26 -0.8 650,246 - B158DRAFT_2467 0.27 -0.3 650,317 - B158DRAFT_2467 0.30 -0.5 650,490 + B158DRAFT_2467 0.39 +0.1 650,490 + B158DRAFT_2467 0.39 +1.3 650,495 + B158DRAFT_2467 0.39 +0.6 650,505 + B158DRAFT_2467 0.40 +0.6 650,505 + B158DRAFT_2467 0.40 +0.8 650,818 - B158DRAFT_2467 0.56 -3.0 650,902 + B158DRAFT_2467 0.60 -1.2 651,063 + B158DRAFT_2467 0.68 -1.6 651,071 - B158DRAFT_2467 0.69 -0.7 651,089 + B158DRAFT_2467 0.70 -0.9 651,097 - B158DRAFT_2467 0.70 -0.5 651,097 - B158DRAFT_2467 0.70 +0.2 651,159 + B158DRAFT_2467 0.73 +0.6 651,281 - B158DRAFT_2467 0.80 +0.1 651,325 - B158DRAFT_2467 0.82 -0.1 651,405 - B158DRAFT_2467 0.86 +0.3 651,407 + B158DRAFT_2467 0.86 -4.6 651,407 + B158DRAFT_2467 0.86 +1.2 651,514 + -0.2 651,627 + +0.7 651,627 + +0.3 651,627 + -0.4 651,635 - +0.1 651,635 - -3.2 651,712 + +0.3 651,712 + +0.3 651,712 + -1.8 651,717 + +0.3 651,717 + +0.3 651,717 + -0.2 651,720 - +1.1 651,722 + -0.2 651,725 - -1.0 651,725 - +0.6 651,725 - +1.5 651,725 - +1.0 651,725 - +0.5 651,725 - -1.5 651,745 - +0.4 651,812 - B158DRAFT_2468 0.22 +0.3 651,863 + B158DRAFT_2468 0.31 -0.5 651,871 - B158DRAFT_2468 0.33 +1.6 651,907 + B158DRAFT_2468 0.39 -3.1 651,923 + B158DRAFT_2468 0.42 -2.1 651,928 + B158DRAFT_2468 0.43 -0.8 651,928 + B158DRAFT_2468 0.43 -0.6 651,931 - B158DRAFT_2468 0.44 -0.1 651,933 + B158DRAFT_2468 0.44 +0.6 651,933 + B158DRAFT_2468 0.44 +0.2 651,933 + B158DRAFT_2468 0.44 -2.1 651,933 + B158DRAFT_2468 0.44 -5.5 651,941 - B158DRAFT_2468 0.45 -2.3 651,941 - B158DRAFT_2468 0.45 -0.9 651,941 - B158DRAFT_2468 0.45 +0.1 651,941 - B158DRAFT_2468 0.45 +0.6 651,941 - B158DRAFT_2468 0.45 +0.5 651,963 - B158DRAFT_2468 0.49 +0.3 651,965 - B158DRAFT_2468 0.50 -0.1 652,049 - B158DRAFT_2468 0.65 +0.9 652,075 + B158DRAFT_2468 0.70 -4.8 652,089 + B158DRAFT_2468 0.72 +0.3 652,141 + B158DRAFT_2468 0.82 -1.5 652,274 + -3.2 652,274 + -5.2 652,280 + -0.2 652,280 + -1.3 652,280 + -4.7 652,280 + -1.0 652,288 - +0.9 652,288 - -0.2 652,288 - -0.4 652,359 + B158DRAFT_2469 0.20 +0.8 652,452 + B158DRAFT_2469 0.38 +0.6 652,480 + B158DRAFT_2469 0.43 -1.1 652,495 - B158DRAFT_2469 0.46 +0.0 652,497 + B158DRAFT_2469 0.46 +0.6 652,497 + B158DRAFT_2469 0.46 -0.1 652,497 + B158DRAFT_2469 0.46 -0.1 652,497 + B158DRAFT_2469 0.46 +0.2 652,497 + B158DRAFT_2469 0.46 -0.0 652,505 - B158DRAFT_2469 0.48 +0.5 652,505 - B158DRAFT_2469 0.48 -0.1 652,505 - B158DRAFT_2469 0.48 -2.3 652,655 - B158DRAFT_2469 0.76 -4.3 652,660 + B158DRAFT_2469 0.77 +0.7 652,660 + B158DRAFT_2469 0.77 -0.2 652,660 + B158DRAFT_2469 0.77 -0.2 652,668 - B158DRAFT_2469 0.79 -3.7
Or see this region's nucleotide sequence