Experiment: m.b. Polymyxin B sulfate 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2287 and B158DRAFT_2288 are separated by 23 nucleotides B158DRAFT_2288 and B158DRAFT_2289 overlap by 4 nucleotides B158DRAFT_2289 and B158DRAFT_2290 overlap by 4 nucleotides
B158DRAFT_2287: B158DRAFT_2287 - ATP phosphoribosyltransferase, at 451,971 to 452,819
_2287
B158DRAFT_2288: B158DRAFT_2288 - haloacid dehalogenase superfamily, subfamily IA, variant 1 with third motif having Dx(3-4)D or Dx(3-4)E, at 452,843 to 454,540
_2288
B158DRAFT_2289: B158DRAFT_2289 - imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 454,537 to 455,133
_2289
B158DRAFT_2290: B158DRAFT_2290 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 455,130 to 455,738
_2290
Position (kb)
452
453
454
455 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 451.888 kb on + strand at 451.912 kb on - strand at 452.082 kb on + strand, within B158DRAFT_2287 at 452.118 kb on + strand, within B158DRAFT_2287 at 452.118 kb on + strand, within B158DRAFT_2287 at 452.166 kb on - strand, within B158DRAFT_2287 at 452.203 kb on + strand, within B158DRAFT_2287 at 452.211 kb on - strand, within B158DRAFT_2287 at 452.228 kb on + strand, within B158DRAFT_2287 at 452.259 kb on + strand, within B158DRAFT_2287 at 452.259 kb on + strand, within B158DRAFT_2287 at 452.259 kb on + strand, within B158DRAFT_2287 at 452.289 kb on - strand, within B158DRAFT_2287 at 452.369 kb on + strand, within B158DRAFT_2287 at 452.536 kb on - strand, within B158DRAFT_2287 at 452.583 kb on + strand, within B158DRAFT_2287 at 452.583 kb on + strand, within B158DRAFT_2287 at 452.583 kb on + strand, within B158DRAFT_2287 at 452.585 kb on + strand, within B158DRAFT_2287 at 452.591 kb on - strand, within B158DRAFT_2287 at 452.592 kb on - strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.623 kb on + strand, within B158DRAFT_2287 at 452.631 kb on - strand, within B158DRAFT_2287 at 452.631 kb on - strand, within B158DRAFT_2287 at 452.646 kb on + strand, within B158DRAFT_2287 at 452.700 kb on + strand, within B158DRAFT_2287 at 452.832 kb on + strand at 452.832 kb on + strand at 452.832 kb on + strand at 452.832 kb on + strand at 452.883 kb on + strand at 452.916 kb on + strand at 452.916 kb on + strand at 452.947 kb on - strand at 452.953 kb on + strand at 452.953 kb on + strand at 452.958 kb on + strand at 452.958 kb on + strand at 452.958 kb on + strand at 452.962 kb on + strand at 452.966 kb on - strand at 453.018 kb on + strand, within B158DRAFT_2288 at 453.075 kb on + strand, within B158DRAFT_2288 at 453.075 kb on + strand, within B158DRAFT_2288 at 453.083 kb on - strand, within B158DRAFT_2288 at 453.083 kb on - strand, within B158DRAFT_2288 at 453.132 kb on + strand, within B158DRAFT_2288 at 453.140 kb on - strand, within B158DRAFT_2288 at 453.168 kb on - strand, within B158DRAFT_2288 at 453.205 kb on + strand, within B158DRAFT_2288 at 453.250 kb on + strand, within B158DRAFT_2288 at 453.262 kb on - strand, within B158DRAFT_2288 at 453.276 kb on + strand, within B158DRAFT_2288 at 453.286 kb on + strand, within B158DRAFT_2288 at 453.294 kb on - strand, within B158DRAFT_2288 at 453.294 kb on - strand, within B158DRAFT_2288 at 453.374 kb on - strand, within B158DRAFT_2288 at 453.406 kb on + strand, within B158DRAFT_2288 at 453.406 kb on + strand, within B158DRAFT_2288 at 453.415 kb on + strand, within B158DRAFT_2288 at 453.415 kb on + strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.423 kb on - strand, within B158DRAFT_2288 at 453.435 kb on - strand, within B158DRAFT_2288 at 453.467 kb on - strand, within B158DRAFT_2288 at 453.487 kb on + strand, within B158DRAFT_2288 at 453.572 kb on - strand, within B158DRAFT_2288 at 453.779 kb on - strand, within B158DRAFT_2288 at 453.790 kb on + strand, within B158DRAFT_2288 at 453.790 kb on + strand, within B158DRAFT_2288 at 453.798 kb on - strand, within B158DRAFT_2288 at 453.803 kb on - strand, within B158DRAFT_2288 at 453.849 kb on - strand, within B158DRAFT_2288 at 453.861 kb on + strand, within B158DRAFT_2288 at 453.878 kb on + strand, within B158DRAFT_2288 at 453.888 kb on + strand, within B158DRAFT_2288 at 453.946 kb on + strand, within B158DRAFT_2288 at 454.041 kb on + strand, within B158DRAFT_2288 at 454.041 kb on + strand, within B158DRAFT_2288 at 454.084 kb on - strand, within B158DRAFT_2288 at 454.105 kb on + strand, within B158DRAFT_2288 at 454.113 kb on - strand, within B158DRAFT_2288 at 454.113 kb on - strand, within B158DRAFT_2288 at 454.142 kb on + strand, within B158DRAFT_2288 at 454.142 kb on + strand, within B158DRAFT_2288 at 454.142 kb on + strand, within B158DRAFT_2288 at 454.142 kb on + strand, within B158DRAFT_2288 at 454.142 kb on + strand, within B158DRAFT_2288 at 454.142 kb on + strand, within B158DRAFT_2288 at 454.142 kb on + strand, within B158DRAFT_2288 at 454.150 kb on - strand, within B158DRAFT_2288 at 454.150 kb on - strand, within B158DRAFT_2288 at 454.150 kb on - strand, within B158DRAFT_2288 at 454.150 kb on - strand, within B158DRAFT_2288 at 454.173 kb on + strand, within B158DRAFT_2288 at 454.173 kb on + strand, within B158DRAFT_2288 at 454.173 kb on + strand, within B158DRAFT_2288 at 454.173 kb on + strand, within B158DRAFT_2288 at 454.173 kb on + strand, within B158DRAFT_2288 at 454.173 kb on + strand, within B158DRAFT_2288 at 454.173 kb on + strand, within B158DRAFT_2288 at 454.181 kb on - strand, within B158DRAFT_2288 at 454.181 kb on - strand, within B158DRAFT_2288 at 454.181 kb on - strand, within B158DRAFT_2288 at 454.181 kb on - strand, within B158DRAFT_2288 at 454.181 kb on - strand, within B158DRAFT_2288 at 454.181 kb on - strand, within B158DRAFT_2288 at 454.324 kb on + strand, within B158DRAFT_2288 at 454.324 kb on + strand, within B158DRAFT_2288 at 454.324 kb on + strand, within B158DRAFT_2288 at 454.324 kb on + strand, within B158DRAFT_2288 at 454.324 kb on + strand, within B158DRAFT_2288 at 454.332 kb on - strand, within B158DRAFT_2288 at 454.332 kb on - strand, within B158DRAFT_2288 at 454.332 kb on - strand, within B158DRAFT_2288 at 454.352 kb on - strand, within B158DRAFT_2288 at 454.352 kb on - strand, within B158DRAFT_2288 at 454.361 kb on + strand, within B158DRAFT_2288 at 454.419 kb on - strand at 454.463 kb on - strand at 454.612 kb on - strand, within B158DRAFT_2289 at 454.641 kb on + strand, within B158DRAFT_2289 at 454.646 kb on + strand, within B158DRAFT_2289 at 454.667 kb on + strand, within B158DRAFT_2289 at 454.797 kb on + strand, within B158DRAFT_2289 at 454.797 kb on + strand, within B158DRAFT_2289 at 454.805 kb on - strand, within B158DRAFT_2289 at 454.858 kb on + strand, within B158DRAFT_2289 at 454.863 kb on + strand, within B158DRAFT_2289 at 454.866 kb on - strand, within B158DRAFT_2289 at 454.874 kb on + strand, within B158DRAFT_2289 at 454.876 kb on + strand, within B158DRAFT_2289 at 454.968 kb on + strand, within B158DRAFT_2289 at 454.969 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.007 kb on + strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.015 kb on - strand, within B158DRAFT_2289 at 455.044 kb on + strand, within B158DRAFT_2289 at 455.069 kb on + strand, within B158DRAFT_2289 at 455.077 kb on - strand at 455.093 kb on + strand at 455.122 kb on - strand at 455.129 kb on - strand at 455.137 kb on + strand at 455.137 kb on + strand at 455.224 kb on + strand, within B158DRAFT_2290 at 455.230 kb on + strand, within B158DRAFT_2290 at 455.246 kb on - strand, within B158DRAFT_2290 at 455.276 kb on + strand, within B158DRAFT_2290 at 455.279 kb on - strand, within B158DRAFT_2290 at 455.349 kb on - strand, within B158DRAFT_2290 at 455.391 kb on + strand, within B158DRAFT_2290 at 455.398 kb on + strand, within B158DRAFT_2290 at 455.409 kb on + strand, within B158DRAFT_2290 at 455.412 kb on - strand, within B158DRAFT_2290 at 455.412 kb on - strand, within B158DRAFT_2290 at 455.420 kb on - strand, within B158DRAFT_2290
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.00390625 mg/ml remove 451,888 + +0.2 451,912 - -1.4 452,082 + B158DRAFT_2287 0.13 +1.2 452,118 + B158DRAFT_2287 0.17 +0.9 452,118 + B158DRAFT_2287 0.17 -0.7 452,166 - B158DRAFT_2287 0.23 +1.3 452,203 + B158DRAFT_2287 0.27 +0.6 452,211 - B158DRAFT_2287 0.28 -0.5 452,228 + B158DRAFT_2287 0.30 -0.5 452,259 + B158DRAFT_2287 0.34 -0.2 452,259 + B158DRAFT_2287 0.34 -0.4 452,259 + B158DRAFT_2287 0.34 -0.9 452,289 - B158DRAFT_2287 0.37 +1.1 452,369 + B158DRAFT_2287 0.47 -3.4 452,536 - B158DRAFT_2287 0.67 +0.4 452,583 + B158DRAFT_2287 0.72 +1.4 452,583 + B158DRAFT_2287 0.72 +0.2 452,583 + B158DRAFT_2287 0.72 -1.0 452,585 + B158DRAFT_2287 0.72 +1.8 452,591 - B158DRAFT_2287 0.73 -0.4 452,592 - B158DRAFT_2287 0.73 -1.9 452,623 + B158DRAFT_2287 0.77 -0.9 452,623 + B158DRAFT_2287 0.77 +0.1 452,623 + B158DRAFT_2287 0.77 +0.1 452,623 + B158DRAFT_2287 0.77 +0.0 452,623 + B158DRAFT_2287 0.77 +0.2 452,623 + B158DRAFT_2287 0.77 +0.1 452,631 - B158DRAFT_2287 0.78 -2.3 452,631 - B158DRAFT_2287 0.78 -0.0 452,646 + B158DRAFT_2287 0.80 -3.6 452,700 + B158DRAFT_2287 0.86 -2.4 452,832 + -0.6 452,832 + +0.2 452,832 + -2.7 452,832 + -1.1 452,883 + -2.2 452,916 + +1.3 452,916 + +0.8 452,947 - -2.4 452,953 + +0.3 452,953 + +0.2 452,958 + -0.7 452,958 + -3.4 452,958 + +0.6 452,962 + +0.3 452,966 - -0.9 453,018 + B158DRAFT_2288 0.10 -1.5 453,075 + B158DRAFT_2288 0.14 -1.6 453,075 + B158DRAFT_2288 0.14 +0.1 453,083 - B158DRAFT_2288 0.14 -0.4 453,083 - B158DRAFT_2288 0.14 -0.2 453,132 + B158DRAFT_2288 0.17 +0.3 453,140 - B158DRAFT_2288 0.17 +0.0 453,168 - B158DRAFT_2288 0.19 -3.8 453,205 + B158DRAFT_2288 0.21 -0.9 453,250 + B158DRAFT_2288 0.24 -0.4 453,262 - B158DRAFT_2288 0.25 -3.1 453,276 + B158DRAFT_2288 0.26 -0.5 453,286 + B158DRAFT_2288 0.26 -0.1 453,294 - B158DRAFT_2288 0.27 -2.2 453,294 - B158DRAFT_2288 0.27 -0.3 453,374 - B158DRAFT_2288 0.31 -0.3 453,406 + B158DRAFT_2288 0.33 +0.5 453,406 + B158DRAFT_2288 0.33 -1.2 453,415 + B158DRAFT_2288 0.34 +0.2 453,415 + B158DRAFT_2288 0.34 -0.5 453,423 - B158DRAFT_2288 0.34 -0.2 453,423 - B158DRAFT_2288 0.34 -1.7 453,423 - B158DRAFT_2288 0.34 -1.6 453,423 - B158DRAFT_2288 0.34 -0.6 453,423 - B158DRAFT_2288 0.34 -4.4 453,435 - B158DRAFT_2288 0.35 -0.6 453,467 - B158DRAFT_2288 0.37 +0.4 453,487 + B158DRAFT_2288 0.38 -2.1 453,572 - B158DRAFT_2288 0.43 -0.6 453,779 - B158DRAFT_2288 0.55 +0.8 453,790 + B158DRAFT_2288 0.56 -0.8 453,790 + B158DRAFT_2288 0.56 -1.0 453,798 - B158DRAFT_2288 0.56 -5.2 453,803 - B158DRAFT_2288 0.57 -1.0 453,849 - B158DRAFT_2288 0.59 -1.4 453,861 + B158DRAFT_2288 0.60 -0.9 453,878 + B158DRAFT_2288 0.61 +0.5 453,888 + B158DRAFT_2288 0.62 +1.6 453,946 + B158DRAFT_2288 0.65 +0.1 454,041 + B158DRAFT_2288 0.71 -4.1 454,041 + B158DRAFT_2288 0.71 +0.4 454,084 - B158DRAFT_2288 0.73 -3.5 454,105 + B158DRAFT_2288 0.74 +0.0 454,113 - B158DRAFT_2288 0.75 -0.9 454,113 - B158DRAFT_2288 0.75 -1.1 454,142 + B158DRAFT_2288 0.77 +0.6 454,142 + B158DRAFT_2288 0.77 +0.6 454,142 + B158DRAFT_2288 0.77 -2.8 454,142 + B158DRAFT_2288 0.77 -0.8 454,142 + B158DRAFT_2288 0.77 +0.3 454,142 + B158DRAFT_2288 0.77 +0.8 454,142 + B158DRAFT_2288 0.77 -1.4 454,150 - B158DRAFT_2288 0.77 +0.3 454,150 - B158DRAFT_2288 0.77 +1.0 454,150 - B158DRAFT_2288 0.77 -4.0 454,150 - B158DRAFT_2288 0.77 -1.3 454,173 + B158DRAFT_2288 0.78 +0.3 454,173 + B158DRAFT_2288 0.78 +0.3 454,173 + B158DRAFT_2288 0.78 -0.4 454,173 + B158DRAFT_2288 0.78 +0.4 454,173 + B158DRAFT_2288 0.78 -0.2 454,173 + B158DRAFT_2288 0.78 -0.2 454,173 + B158DRAFT_2288 0.78 -0.5 454,181 - B158DRAFT_2288 0.79 -3.6 454,181 - B158DRAFT_2288 0.79 +0.4 454,181 - B158DRAFT_2288 0.79 +0.4 454,181 - B158DRAFT_2288 0.79 +1.7 454,181 - B158DRAFT_2288 0.79 +1.9 454,181 - B158DRAFT_2288 0.79 -0.1 454,324 + B158DRAFT_2288 0.87 +0.1 454,324 + B158DRAFT_2288 0.87 -0.7 454,324 + B158DRAFT_2288 0.87 -2.5 454,324 + B158DRAFT_2288 0.87 -3.0 454,324 + B158DRAFT_2288 0.87 +0.5 454,332 - B158DRAFT_2288 0.88 +0.0 454,332 - B158DRAFT_2288 0.88 +0.9 454,332 - B158DRAFT_2288 0.88 -0.7 454,352 - B158DRAFT_2288 0.89 +0.4 454,352 - B158DRAFT_2288 0.89 -3.3 454,361 + B158DRAFT_2288 0.89 +0.3 454,419 - +1.5 454,463 - -0.5 454,612 - B158DRAFT_2289 0.13 -0.8 454,641 + B158DRAFT_2289 0.17 +1.1 454,646 + B158DRAFT_2289 0.18 -0.8 454,667 + B158DRAFT_2289 0.22 +0.1 454,797 + B158DRAFT_2289 0.44 -0.6 454,797 + B158DRAFT_2289 0.44 +0.9 454,805 - B158DRAFT_2289 0.45 +0.4 454,858 + B158DRAFT_2289 0.54 -0.2 454,863 + B158DRAFT_2289 0.55 -1.0 454,866 - B158DRAFT_2289 0.55 -0.1 454,874 + B158DRAFT_2289 0.56 +1.5 454,876 + B158DRAFT_2289 0.57 -0.1 454,968 + B158DRAFT_2289 0.72 -1.1 454,969 + B158DRAFT_2289 0.72 -0.9 455,007 + B158DRAFT_2289 0.79 +0.3 455,007 + B158DRAFT_2289 0.79 -0.2 455,007 + B158DRAFT_2289 0.79 +0.0 455,007 + B158DRAFT_2289 0.79 -3.1 455,015 - B158DRAFT_2289 0.80 -0.1 455,015 - B158DRAFT_2289 0.80 -3.4 455,015 - B158DRAFT_2289 0.80 +0.1 455,015 - B158DRAFT_2289 0.80 +0.1 455,044 + B158DRAFT_2289 0.85 -2.9 455,069 + B158DRAFT_2289 0.89 +0.7 455,077 - +1.1 455,093 + -0.0 455,122 - -1.5 455,129 - -0.7 455,137 + -0.2 455,137 + -4.6 455,224 + B158DRAFT_2290 0.15 +1.2 455,230 + B158DRAFT_2290 0.16 +1.1 455,246 - B158DRAFT_2290 0.19 +0.8 455,276 + B158DRAFT_2290 0.24 +0.3 455,279 - B158DRAFT_2290 0.24 -0.1 455,349 - B158DRAFT_2290 0.36 +1.3 455,391 + B158DRAFT_2290 0.43 +0.7 455,398 + B158DRAFT_2290 0.44 +0.6 455,409 + B158DRAFT_2290 0.46 +1.3 455,412 - B158DRAFT_2290 0.46 -0.9 455,412 - B158DRAFT_2290 0.46 +0.4 455,420 - B158DRAFT_2290 0.48 +0.1
Or see this region's nucleotide sequence