Experiment: m.b. Polymyxin B sulfate 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2175 and B158DRAFT_2176 are separated by 75 nucleotides B158DRAFT_2176 and B158DRAFT_2177 are separated by 83 nucleotides B158DRAFT_2177 and B158DRAFT_2178 are separated by 29 nucleotides B158DRAFT_2178 and B158DRAFT_2179 overlap by 10 nucleotides
B158DRAFT_2175: B158DRAFT_2175 - Uncharacterized protein conserved in bacteria, at 321,845 to 323,692
_2175
B158DRAFT_2176: B158DRAFT_2176 - folate-binding protein YgfZ, at 323,768 to 324,706
_2176
B158DRAFT_2177: B158DRAFT_2177 - Uncharacterized conserved protein, at 324,790 to 325,044
_2177
B158DRAFT_2178: B158DRAFT_2178 - hypothetical protein, at 325,074 to 325,580
_2178
B158DRAFT_2179: B158DRAFT_2179 - L-aspartate oxidase (EC 1.4.3.16), at 325,571 to 327,187
_2179
Position (kb) 323
324
325 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 322.830 kb on + strand, within B158DRAFT_2175 at 322.888 kb on + strand, within B158DRAFT_2175 at 322.950 kb on + strand, within B158DRAFT_2175 at 322.960 kb on + strand, within B158DRAFT_2175 at 322.960 kb on + strand, within B158DRAFT_2175 at 322.968 kb on - strand, within B158DRAFT_2175 at 322.968 kb on - strand, within B158DRAFT_2175 at 322.968 kb on - strand, within B158DRAFT_2175 at 322.970 kb on + strand, within B158DRAFT_2175 at 323.028 kb on - strand, within B158DRAFT_2175 at 323.049 kb on - strand, within B158DRAFT_2175 at 323.063 kb on - strand, within B158DRAFT_2175 at 323.082 kb on - strand, within B158DRAFT_2175 at 323.093 kb on + strand, within B158DRAFT_2175 at 323.120 kb on - strand, within B158DRAFT_2175 at 323.131 kb on + strand, within B158DRAFT_2175 at 323.270 kb on + strand, within B158DRAFT_2175 at 323.318 kb on - strand, within B158DRAFT_2175 at 323.318 kb on - strand, within B158DRAFT_2175 at 323.318 kb on - strand, within B158DRAFT_2175 at 323.329 kb on - strand, within B158DRAFT_2175 at 323.399 kb on + strand, within B158DRAFT_2175 at 323.399 kb on + strand, within B158DRAFT_2175 at 323.409 kb on + strand, within B158DRAFT_2175 at 323.409 kb on + strand, within B158DRAFT_2175 at 323.425 kb on - strand, within B158DRAFT_2175 at 323.527 kb on + strand at 323.629 kb on + strand at 323.760 kb on + strand at 324.658 kb on - strand at 324.738 kb on + strand at 324.754 kb on - strand at 324.754 kb on - strand at 325.100 kb on + strand at 325.100 kb on + strand at 325.108 kb on - strand at 325.137 kb on + strand, within B158DRAFT_2178 at 325.153 kb on + strand, within B158DRAFT_2178 at 325.153 kb on + strand, within B158DRAFT_2178 at 325.186 kb on - strand, within B158DRAFT_2178 at 325.257 kb on + strand, within B158DRAFT_2178 at 325.315 kb on + strand, within B158DRAFT_2178 at 325.332 kb on - strand, within B158DRAFT_2178 at 325.444 kb on + strand, within B158DRAFT_2178 at 325.531 kb on - strand at 325.534 kb on - strand at 325.534 kb on - strand at 325.591 kb on + strand at 325.612 kb on - strand at 325.637 kb on - strand at 325.641 kb on + strand at 325.641 kb on + strand at 325.649 kb on - strand at 325.649 kb on - strand at 325.649 kb on - strand at 325.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.00390625 mg/ml remove 322,830 + B158DRAFT_2175 0.53 -0.5 322,888 + B158DRAFT_2175 0.56 -4.5 322,950 + B158DRAFT_2175 0.60 -0.6 322,960 + B158DRAFT_2175 0.60 -0.3 322,960 + B158DRAFT_2175 0.60 -0.9 322,968 - B158DRAFT_2175 0.61 +0.0 322,968 - B158DRAFT_2175 0.61 +1.1 322,968 - B158DRAFT_2175 0.61 -0.1 322,970 + B158DRAFT_2175 0.61 +0.6 323,028 - B158DRAFT_2175 0.64 -0.7 323,049 - B158DRAFT_2175 0.65 -0.5 323,063 - B158DRAFT_2175 0.66 +0.5 323,082 - B158DRAFT_2175 0.67 -4.5 323,093 + B158DRAFT_2175 0.68 -2.0 323,120 - B158DRAFT_2175 0.69 -0.5 323,131 + B158DRAFT_2175 0.70 -4.9 323,270 + B158DRAFT_2175 0.77 -0.2 323,318 - B158DRAFT_2175 0.80 -2.1 323,318 - B158DRAFT_2175 0.80 -0.6 323,318 - B158DRAFT_2175 0.80 -4.2 323,329 - B158DRAFT_2175 0.80 -0.6 323,399 + B158DRAFT_2175 0.84 +0.2 323,399 + B158DRAFT_2175 0.84 +1.9 323,409 + B158DRAFT_2175 0.85 +0.7 323,409 + B158DRAFT_2175 0.85 -0.0 323,425 - B158DRAFT_2175 0.85 -0.6 323,527 + +0.8 323,629 + -4.6 323,760 + +0.2 324,658 - -2.8 324,738 + +0.8 324,754 - -2.2 324,754 - -0.6 325,100 + +0.2 325,100 + -1.5 325,108 - +0.9 325,137 + B158DRAFT_2178 0.12 +0.9 325,153 + B158DRAFT_2178 0.16 +0.7 325,153 + B158DRAFT_2178 0.16 +0.0 325,186 - B158DRAFT_2178 0.22 +0.2 325,257 + B158DRAFT_2178 0.36 +0.3 325,315 + B158DRAFT_2178 0.48 -0.5 325,332 - B158DRAFT_2178 0.51 -0.9 325,444 + B158DRAFT_2178 0.73 -3.7 325,531 - -0.1 325,534 - +0.4 325,534 - -0.1 325,591 + -0.2 325,612 - -2.2 325,637 - +0.9 325,641 + +0.2 325,641 + -2.8 325,649 - -3.7 325,649 - +2.7 325,649 - -0.3 325,680 - -2.9
Or see this region's nucleotide sequence