Experiment: m.b. Polymyxin B sulfate 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2156 and B158DRAFT_2157 are separated by 457 nucleotides B158DRAFT_2157 and B158DRAFT_2158 are separated by 147 nucleotides
B158DRAFT_2156: B158DRAFT_2156 - Uncharacterized membrane protein, required for spore maturation in B.subtilis., at 294,657 to 295,886
_2156
B158DRAFT_2157: B158DRAFT_2157 - 16S RNA G1207 methylase RsmC, at 296,344 to 296,937
_2157
B158DRAFT_2158: B158DRAFT_2158 - Predicted SAM-dependent methyltransferase, at 297,085 to 298,161
_2158
Position (kb)
296
297 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 295.346 kb on - strand, within B158DRAFT_2156 at 295.347 kb on + strand, within B158DRAFT_2156 at 295.352 kb on + strand, within B158DRAFT_2156 at 295.395 kb on - strand, within B158DRAFT_2156 at 295.434 kb on + strand, within B158DRAFT_2156 at 295.456 kb on + strand, within B158DRAFT_2156 at 295.456 kb on + strand, within B158DRAFT_2156 at 295.528 kb on + strand, within B158DRAFT_2156 at 295.535 kb on - strand, within B158DRAFT_2156 at 295.566 kb on + strand, within B158DRAFT_2156 at 295.571 kb on + strand, within B158DRAFT_2156 at 295.574 kb on - strand, within B158DRAFT_2156 at 295.579 kb on - strand, within B158DRAFT_2156 at 295.611 kb on + strand, within B158DRAFT_2156 at 295.617 kb on + strand, within B158DRAFT_2156 at 295.617 kb on + strand, within B158DRAFT_2156 at 295.619 kb on - strand, within B158DRAFT_2156 at 295.619 kb on - strand, within B158DRAFT_2156 at 295.630 kb on - strand, within B158DRAFT_2156 at 295.642 kb on - strand, within B158DRAFT_2156 at 295.654 kb on + strand, within B158DRAFT_2156 at 295.662 kb on - strand, within B158DRAFT_2156 at 295.662 kb on - strand, within B158DRAFT_2156 at 295.664 kb on + strand, within B158DRAFT_2156 at 295.664 kb on + strand, within B158DRAFT_2156 at 295.665 kb on + strand, within B158DRAFT_2156 at 295.673 kb on + strand, within B158DRAFT_2156 at 295.673 kb on + strand, within B158DRAFT_2156 at 295.731 kb on + strand, within B158DRAFT_2156 at 295.731 kb on + strand, within B158DRAFT_2156 at 295.807 kb on - strand at 295.807 kb on - strand at 295.809 kb on + strand at 295.817 kb on - strand at 295.867 kb on - strand at 295.898 kb on - strand at 296.061 kb on - strand at 296.267 kb on + strand at 296.323 kb on + strand at 296.334 kb on + strand at 296.342 kb on - strand at 296.374 kb on + strand at 296.392 kb on - strand at 296.585 kb on + strand, within B158DRAFT_2157 at 296.599 kb on + strand, within B158DRAFT_2157 at 296.604 kb on + strand, within B158DRAFT_2157 at 296.615 kb on - strand, within B158DRAFT_2157 at 296.627 kb on + strand, within B158DRAFT_2157 at 296.627 kb on + strand, within B158DRAFT_2157 at 296.635 kb on - strand, within B158DRAFT_2157 at 296.659 kb on - strand, within B158DRAFT_2157 at 296.733 kb on - strand, within B158DRAFT_2157 at 296.734 kb on + strand, within B158DRAFT_2157 at 296.741 kb on + strand, within B158DRAFT_2157 at 296.742 kb on - strand, within B158DRAFT_2157 at 296.749 kb on - strand, within B158DRAFT_2157 at 296.751 kb on + strand, within B158DRAFT_2157 at 296.751 kb on + strand, within B158DRAFT_2157 at 296.751 kb on + strand, within B158DRAFT_2157 at 296.769 kb on - strand, within B158DRAFT_2157 at 296.830 kb on + strand, within B158DRAFT_2157 at 296.835 kb on + strand, within B158DRAFT_2157 at 296.838 kb on - strand, within B158DRAFT_2157 at 296.939 kb on + strand at 296.939 kb on + strand at 296.945 kb on + strand at 296.945 kb on + strand at 296.951 kb on + strand at 296.953 kb on - strand at 296.959 kb on - strand at 296.959 kb on - strand at 297.000 kb on - strand at 297.016 kb on - strand at 297.121 kb on + strand at 297.132 kb on + strand at 297.132 kb on + strand at 297.137 kb on + strand at 297.172 kb on + strand at 297.185 kb on + strand at 297.202 kb on + strand, within B158DRAFT_2158 at 297.226 kb on - strand, within B158DRAFT_2158 at 297.235 kb on - strand, within B158DRAFT_2158 at 297.311 kb on + strand, within B158DRAFT_2158 at 297.358 kb on - strand, within B158DRAFT_2158 at 297.363 kb on + strand, within B158DRAFT_2158 at 297.364 kb on - strand, within B158DRAFT_2158 at 297.378 kb on + strand, within B158DRAFT_2158 at 297.379 kb on - strand, within B158DRAFT_2158 at 297.381 kb on - strand, within B158DRAFT_2158 at 297.381 kb on - strand, within B158DRAFT_2158 at 297.386 kb on - strand, within B158DRAFT_2158 at 297.401 kb on - strand, within B158DRAFT_2158 at 297.406 kb on + strand, within B158DRAFT_2158 at 297.414 kb on - strand, within B158DRAFT_2158 at 297.424 kb on - strand, within B158DRAFT_2158 at 297.425 kb on + strand, within B158DRAFT_2158 at 297.425 kb on + strand, within B158DRAFT_2158 at 297.425 kb on + strand, within B158DRAFT_2158 at 297.463 kb on + strand, within B158DRAFT_2158 at 297.463 kb on + strand, within B158DRAFT_2158 at 297.471 kb on - strand, within B158DRAFT_2158 at 297.554 kb on - strand, within B158DRAFT_2158 at 297.643 kb on + strand, within B158DRAFT_2158 at 297.651 kb on - strand, within B158DRAFT_2158 at 297.652 kb on - strand, within B158DRAFT_2158 at 297.721 kb on + strand, within B158DRAFT_2158 at 297.721 kb on + strand, within B158DRAFT_2158 at 297.721 kb on + strand, within B158DRAFT_2158 at 297.721 kb on + strand, within B158DRAFT_2158 at 297.729 kb on - strand, within B158DRAFT_2158
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.00390625 mg/ml remove 295,346 - B158DRAFT_2156 0.56 -1.0 295,347 + B158DRAFT_2156 0.56 -1.4 295,352 + B158DRAFT_2156 0.57 +0.2 295,395 - B158DRAFT_2156 0.60 -0.2 295,434 + B158DRAFT_2156 0.63 -0.9 295,456 + B158DRAFT_2156 0.65 -1.4 295,456 + B158DRAFT_2156 0.65 +0.3 295,528 + B158DRAFT_2156 0.71 -4.4 295,535 - B158DRAFT_2156 0.71 -0.2 295,566 + B158DRAFT_2156 0.74 -1.5 295,571 + B158DRAFT_2156 0.74 -4.5 295,574 - B158DRAFT_2156 0.75 +0.3 295,579 - B158DRAFT_2156 0.75 -0.2 295,611 + B158DRAFT_2156 0.78 -1.1 295,617 + B158DRAFT_2156 0.78 +0.2 295,617 + B158DRAFT_2156 0.78 -0.4 295,619 - B158DRAFT_2156 0.78 +0.1 295,619 - B158DRAFT_2156 0.78 -0.0 295,630 - B158DRAFT_2156 0.79 -1.8 295,642 - B158DRAFT_2156 0.80 +0.0 295,654 + B158DRAFT_2156 0.81 +0.2 295,662 - B158DRAFT_2156 0.82 -0.3 295,662 - B158DRAFT_2156 0.82 -0.5 295,664 + B158DRAFT_2156 0.82 -3.9 295,664 + B158DRAFT_2156 0.82 +0.1 295,665 + B158DRAFT_2156 0.82 -1.6 295,673 + B158DRAFT_2156 0.83 -0.7 295,673 + B158DRAFT_2156 0.83 -2.8 295,731 + B158DRAFT_2156 0.87 -0.2 295,731 + B158DRAFT_2156 0.87 -0.6 295,807 - +0.8 295,807 - -0.2 295,809 + -0.8 295,817 - -4.3 295,867 - -2.9 295,898 - -5.1 296,061 - -1.1 296,267 + -1.2 296,323 + -0.4 296,334 + -0.2 296,342 - -3.5 296,374 + +0.3 296,392 - -0.2 296,585 + B158DRAFT_2157 0.41 -0.8 296,599 + B158DRAFT_2157 0.43 +0.4 296,604 + B158DRAFT_2157 0.44 -0.8 296,615 - B158DRAFT_2157 0.46 -0.4 296,627 + B158DRAFT_2157 0.48 +0.9 296,627 + B158DRAFT_2157 0.48 +0.3 296,635 - B158DRAFT_2157 0.49 -0.2 296,659 - B158DRAFT_2157 0.53 +0.1 296,733 - B158DRAFT_2157 0.65 -1.9 296,734 + B158DRAFT_2157 0.66 -3.0 296,741 + B158DRAFT_2157 0.67 -2.4 296,742 - B158DRAFT_2157 0.67 -0.2 296,749 - B158DRAFT_2157 0.68 +0.5 296,751 + B158DRAFT_2157 0.69 -2.1 296,751 + B158DRAFT_2157 0.69 +1.0 296,751 + B158DRAFT_2157 0.69 +1.6 296,769 - B158DRAFT_2157 0.72 -1.0 296,830 + B158DRAFT_2157 0.82 +0.9 296,835 + B158DRAFT_2157 0.83 +0.3 296,838 - B158DRAFT_2157 0.83 -1.1 296,939 + -0.8 296,939 + +0.7 296,945 + -0.2 296,945 + -1.4 296,951 + -1.9 296,953 - -0.4 296,959 - +0.1 296,959 - -0.8 297,000 - -1.5 297,016 - +0.6 297,121 + +0.9 297,132 + +0.3 297,132 + +0.1 297,137 + +1.4 297,172 + +0.0 297,185 + +0.1 297,202 + B158DRAFT_2158 0.11 -0.2 297,226 - B158DRAFT_2158 0.13 +0.3 297,235 - B158DRAFT_2158 0.14 +0.0 297,311 + B158DRAFT_2158 0.21 -0.8 297,358 - B158DRAFT_2158 0.25 +0.1 297,363 + B158DRAFT_2158 0.26 +0.0 297,364 - B158DRAFT_2158 0.26 -0.5 297,378 + B158DRAFT_2158 0.27 -0.3 297,379 - B158DRAFT_2158 0.27 +0.4 297,381 - B158DRAFT_2158 0.27 +0.8 297,381 - B158DRAFT_2158 0.27 +0.3 297,386 - B158DRAFT_2158 0.28 -4.0 297,401 - B158DRAFT_2158 0.29 +0.2 297,406 + B158DRAFT_2158 0.30 +0.9 297,414 - B158DRAFT_2158 0.31 -0.3 297,424 - B158DRAFT_2158 0.31 +0.0 297,425 + B158DRAFT_2158 0.32 -1.0 297,425 + B158DRAFT_2158 0.32 -1.5 297,425 + B158DRAFT_2158 0.32 -0.5 297,463 + B158DRAFT_2158 0.35 -1.0 297,463 + B158DRAFT_2158 0.35 -0.8 297,471 - B158DRAFT_2158 0.36 +0.1 297,554 - B158DRAFT_2158 0.44 +0.3 297,643 + B158DRAFT_2158 0.52 -0.8 297,651 - B158DRAFT_2158 0.53 +1.5 297,652 - B158DRAFT_2158 0.53 +0.7 297,721 + B158DRAFT_2158 0.59 -0.5 297,721 + B158DRAFT_2158 0.59 +0.9 297,721 + B158DRAFT_2158 0.59 -0.8 297,721 + B158DRAFT_2158 0.59 -0.6 297,729 - B158DRAFT_2158 0.60 +1.9
Or see this region's nucleotide sequence