Experiment: m.b. Polymyxin B sulfate 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1111 and B158DRAFT_1112 are separated by 253 nucleotides B158DRAFT_1112 and B158DRAFT_1113 are separated by 9 nucleotides
B158DRAFT_1111: B158DRAFT_1111 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 1,147,165 to 1,148,550
_1111
B158DRAFT_1112: B158DRAFT_1112 - acetyl-CoA carboxylase, carboxyl transferase, alpha subunit, at 1,148,804 to 1,149,763
_1112
B158DRAFT_1113: B158DRAFT_1113 - tRNA(Ile)-lysidine synthetase, N-terminal domain/tRNA(Ile)-lysidine synthetase, C-terminal domain, at 1,149,773 to 1,151,047
_1113
Position (kb)
1148
1149
1150 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1147.807 kb on + strand, within B158DRAFT_1111 at 1147.807 kb on + strand, within B158DRAFT_1111 at 1147.815 kb on - strand, within B158DRAFT_1111 at 1147.830 kb on + strand, within B158DRAFT_1111 at 1147.838 kb on - strand, within B158DRAFT_1111 at 1147.855 kb on - strand, within B158DRAFT_1111 at 1147.865 kb on - strand, within B158DRAFT_1111 at 1147.926 kb on - strand, within B158DRAFT_1111 at 1147.926 kb on - strand, within B158DRAFT_1111 at 1147.926 kb on - strand, within B158DRAFT_1111 at 1147.926 kb on - strand, within B158DRAFT_1111 at 1147.926 kb on - strand, within B158DRAFT_1111 at 1147.933 kb on + strand, within B158DRAFT_1111 at 1147.941 kb on - strand, within B158DRAFT_1111 at 1148.022 kb on - strand, within B158DRAFT_1111 at 1148.022 kb on - strand, within B158DRAFT_1111 at 1148.035 kb on + strand, within B158DRAFT_1111 at 1148.059 kb on + strand, within B158DRAFT_1111 at 1148.059 kb on + strand, within B158DRAFT_1111 at 1148.136 kb on - strand, within B158DRAFT_1111 at 1148.136 kb on - strand, within B158DRAFT_1111 at 1148.137 kb on - strand, within B158DRAFT_1111 at 1148.166 kb on + strand, within B158DRAFT_1111 at 1148.173 kb on - strand, within B158DRAFT_1111 at 1148.174 kb on - strand, within B158DRAFT_1111 at 1148.174 kb on - strand, within B158DRAFT_1111 at 1148.232 kb on + strand, within B158DRAFT_1111 at 1148.245 kb on - strand, within B158DRAFT_1111 at 1148.247 kb on + strand, within B158DRAFT_1111 at 1148.247 kb on + strand, within B158DRAFT_1111 at 1148.250 kb on - strand, within B158DRAFT_1111 at 1148.255 kb on + strand, within B158DRAFT_1111 at 1148.255 kb on - strand, within B158DRAFT_1111 at 1148.261 kb on + strand, within B158DRAFT_1111 at 1148.263 kb on - strand, within B158DRAFT_1111 at 1148.263 kb on - strand, within B158DRAFT_1111 at 1148.328 kb on - strand, within B158DRAFT_1111 at 1148.336 kb on + strand, within B158DRAFT_1111 at 1148.342 kb on - strand, within B158DRAFT_1111 at 1148.344 kb on + strand, within B158DRAFT_1111 at 1148.344 kb on + strand, within B158DRAFT_1111 at 1148.344 kb on + strand, within B158DRAFT_1111 at 1148.344 kb on + strand, within B158DRAFT_1111 at 1148.344 kb on + strand, within B158DRAFT_1111 at 1148.352 kb on - strand, within B158DRAFT_1111 at 1148.352 kb on - strand, within B158DRAFT_1111 at 1148.391 kb on + strand, within B158DRAFT_1111 at 1148.412 kb on + strand at 1148.412 kb on + strand at 1148.420 kb on - strand at 1148.424 kb on + strand at 1148.424 kb on + strand at 1148.432 kb on - strand at 1148.437 kb on - strand at 1148.466 kb on - strand at 1148.481 kb on + strand at 1148.503 kb on + strand at 1148.503 kb on + strand at 1148.504 kb on - strand at 1148.713 kb on + strand at 1148.713 kb on + strand at 1148.713 kb on + strand at 1148.713 kb on + strand at 1148.713 kb on + strand at 1148.713 kb on + strand at 1148.721 kb on - strand at 1148.721 kb on - strand at 1148.721 kb on - strand at 1148.721 kb on - strand at 1148.721 kb on - strand at 1148.721 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.00390625 mg/ml remove 1,147,807 + B158DRAFT_1111 0.46 +0.3 1,147,807 + B158DRAFT_1111 0.46 -0.3 1,147,815 - B158DRAFT_1111 0.47 +0.5 1,147,830 + B158DRAFT_1111 0.48 -0.8 1,147,838 - B158DRAFT_1111 0.49 -0.7 1,147,855 - B158DRAFT_1111 0.50 -5.2 1,147,865 - B158DRAFT_1111 0.51 +1.2 1,147,926 - B158DRAFT_1111 0.55 -0.1 1,147,926 - B158DRAFT_1111 0.55 -0.2 1,147,926 - B158DRAFT_1111 0.55 -4.1 1,147,926 - B158DRAFT_1111 0.55 +0.3 1,147,926 - B158DRAFT_1111 0.55 +0.1 1,147,933 + B158DRAFT_1111 0.55 -0.4 1,147,941 - B158DRAFT_1111 0.56 -2.1 1,148,022 - B158DRAFT_1111 0.62 +0.3 1,148,022 - B158DRAFT_1111 0.62 -1.3 1,148,035 + B158DRAFT_1111 0.63 +0.3 1,148,059 + B158DRAFT_1111 0.65 -0.4 1,148,059 + B158DRAFT_1111 0.65 +1.2 1,148,136 - B158DRAFT_1111 0.70 +0.3 1,148,136 - B158DRAFT_1111 0.70 +0.1 1,148,137 - B158DRAFT_1111 0.70 +0.0 1,148,166 + B158DRAFT_1111 0.72 +0.4 1,148,173 - B158DRAFT_1111 0.73 +0.2 1,148,174 - B158DRAFT_1111 0.73 -2.5 1,148,174 - B158DRAFT_1111 0.73 +0.8 1,148,232 + B158DRAFT_1111 0.77 -4.4 1,148,245 - B158DRAFT_1111 0.78 +0.4 1,148,247 + B158DRAFT_1111 0.78 +0.9 1,148,247 + B158DRAFT_1111 0.78 -1.7 1,148,250 - B158DRAFT_1111 0.78 +0.0 1,148,255 + B158DRAFT_1111 0.79 -0.7 1,148,255 - B158DRAFT_1111 0.79 -0.6 1,148,261 + B158DRAFT_1111 0.79 +0.8 1,148,263 - B158DRAFT_1111 0.79 +0.3 1,148,263 - B158DRAFT_1111 0.79 -0.3 1,148,328 - B158DRAFT_1111 0.84 -1.0 1,148,336 + B158DRAFT_1111 0.84 +0.1 1,148,342 - B158DRAFT_1111 0.85 +0.8 1,148,344 + B158DRAFT_1111 0.85 +0.0 1,148,344 + B158DRAFT_1111 0.85 -0.3 1,148,344 + B158DRAFT_1111 0.85 +0.5 1,148,344 + B158DRAFT_1111 0.85 +0.5 1,148,344 + B158DRAFT_1111 0.85 +0.1 1,148,352 - B158DRAFT_1111 0.86 +0.3 1,148,352 - B158DRAFT_1111 0.86 +0.7 1,148,391 + B158DRAFT_1111 0.88 +0.2 1,148,412 + +1.2 1,148,412 + -0.3 1,148,420 - +0.3 1,148,424 + +0.8 1,148,424 + -1.0 1,148,432 - +0.0 1,148,437 - +0.8 1,148,466 - +1.5 1,148,481 + -0.2 1,148,503 + +0.3 1,148,503 + -0.6 1,148,504 - -0.5 1,148,713 + +1.3 1,148,713 + -4.2 1,148,713 + +0.1 1,148,713 + +1.4 1,148,713 + +0.7 1,148,713 + -0.2 1,148,721 - -0.7 1,148,721 - +0.8 1,148,721 - -0.9 1,148,721 - -0.3 1,148,721 - -0.8 1,148,721 - +0.1
Or see this region's nucleotide sequence