Experiment: m.b. Polymyxin B sulfate 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0585 and B158DRAFT_0586 are separated by 25 nucleotides B158DRAFT_0586 and B158DRAFT_0587 are separated by 2 nucleotides B158DRAFT_0587 and B158DRAFT_0588 are separated by 82 nucleotides
B158DRAFT_0585: B158DRAFT_0585 - Short-chain alcohol dehydrogenase of unknown specificity, at 590,866 to 591,648
_0585
B158DRAFT_0586: B158DRAFT_0586 - Uncharacterized protein conserved in bacteria, at 591,674 to 592,102
_0586
B158DRAFT_0587: B158DRAFT_0587 - FKBP-type peptidyl-prolyl cis-trans isomerases 2, at 592,105 to 592,587
_0587
B158DRAFT_0588: B158DRAFT_0588 - ribonuclease PH, at 592,670 to 593,386
_0588
Position (kb)
591
592
593 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 590.715 kb on - strand at 590.730 kb on + strand at 590.738 kb on - strand at 590.808 kb on + strand at 590.808 kb on + strand at 590.816 kb on - strand at 590.816 kb on - strand at 590.816 kb on - strand at 590.816 kb on - strand at 590.816 kb on - strand at 590.854 kb on + strand at 590.917 kb on - strand at 590.920 kb on + strand at 590.920 kb on + strand at 590.920 kb on + strand at 590.920 kb on + strand at 590.920 kb on + strand at 590.920 kb on + strand at 590.920 kb on + strand at 590.928 kb on - strand at 590.928 kb on - strand at 590.928 kb on - strand at 590.928 kb on - strand at 590.928 kb on - strand at 590.928 kb on - strand at 590.928 kb on - strand at 590.972 kb on - strand, within B158DRAFT_0585 at 591.017 kb on + strand, within B158DRAFT_0585 at 591.024 kb on + strand, within B158DRAFT_0585 at 591.078 kb on + strand, within B158DRAFT_0585 at 591.078 kb on + strand, within B158DRAFT_0585 at 591.078 kb on + strand, within B158DRAFT_0585 at 591.078 kb on + strand, within B158DRAFT_0585 at 591.086 kb on - strand, within B158DRAFT_0585 at 591.086 kb on - strand, within B158DRAFT_0585 at 591.086 kb on - strand, within B158DRAFT_0585 at 591.086 kb on - strand, within B158DRAFT_0585 at 591.086 kb on - strand, within B158DRAFT_0585 at 591.086 kb on - strand, within B158DRAFT_0585 at 591.086 kb on - strand, within B158DRAFT_0585 at 591.103 kb on + strand, within B158DRAFT_0585 at 591.132 kb on + strand, within B158DRAFT_0585 at 591.167 kb on - strand, within B158DRAFT_0585 at 591.190 kb on + strand, within B158DRAFT_0585 at 591.190 kb on + strand, within B158DRAFT_0585 at 591.198 kb on - strand, within B158DRAFT_0585 at 591.198 kb on - strand, within B158DRAFT_0585 at 591.398 kb on + strand, within B158DRAFT_0585 at 591.406 kb on - strand, within B158DRAFT_0585 at 591.406 kb on - strand, within B158DRAFT_0585 at 591.408 kb on - strand, within B158DRAFT_0585 at 591.495 kb on + strand, within B158DRAFT_0585 at 591.495 kb on + strand, within B158DRAFT_0585 at 591.502 kb on + strand, within B158DRAFT_0585 at 591.518 kb on + strand, within B158DRAFT_0585 at 591.518 kb on + strand, within B158DRAFT_0585 at 591.518 kb on + strand, within B158DRAFT_0585 at 591.518 kb on + strand, within B158DRAFT_0585 at 591.520 kb on + strand, within B158DRAFT_0585 at 591.526 kb on - strand, within B158DRAFT_0585 at 591.528 kb on - strand, within B158DRAFT_0585 at 591.528 kb on - strand, within B158DRAFT_0585 at 591.543 kb on + strand, within B158DRAFT_0585 at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.609 kb on + strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.617 kb on - strand at 591.651 kb on - strand at 591.651 kb on - strand at 591.651 kb on - strand at 591.653 kb on + strand at 591.661 kb on - strand at 591.733 kb on - strand, within B158DRAFT_0586 at 591.771 kb on - strand, within B158DRAFT_0586 at 591.888 kb on - strand, within B158DRAFT_0586 at 591.913 kb on - strand, within B158DRAFT_0586 at 591.929 kb on - strand, within B158DRAFT_0586 at 591.934 kb on - strand, within B158DRAFT_0586 at 591.934 kb on - strand, within B158DRAFT_0586 at 591.946 kb on - strand, within B158DRAFT_0586 at 592.004 kb on + strand, within B158DRAFT_0586 at 592.012 kb on - strand, within B158DRAFT_0586 at 592.052 kb on + strand, within B158DRAFT_0586 at 592.129 kb on + strand at 592.162 kb on + strand, within B158DRAFT_0587 at 592.162 kb on + strand, within B158DRAFT_0587 at 592.170 kb on - strand, within B158DRAFT_0587 at 592.178 kb on + strand, within B158DRAFT_0587 at 592.178 kb on + strand, within B158DRAFT_0587 at 592.275 kb on + strand, within B158DRAFT_0587 at 592.275 kb on + strand, within B158DRAFT_0587 at 592.275 kb on + strand, within B158DRAFT_0587 at 592.283 kb on - strand, within B158DRAFT_0587 at 592.334 kb on + strand, within B158DRAFT_0587 at 592.334 kb on + strand, within B158DRAFT_0587 at 592.342 kb on - strand, within B158DRAFT_0587 at 592.351 kb on + strand, within B158DRAFT_0587 at 592.374 kb on + strand, within B158DRAFT_0587 at 592.382 kb on - strand, within B158DRAFT_0587 at 592.382 kb on - strand, within B158DRAFT_0587 at 592.382 kb on - strand, within B158DRAFT_0587 at 592.389 kb on - strand, within B158DRAFT_0587 at 592.390 kb on - strand, within B158DRAFT_0587 at 592.421 kb on + strand, within B158DRAFT_0587 at 592.448 kb on + strand, within B158DRAFT_0587 at 592.473 kb on + strand, within B158DRAFT_0587 at 592.473 kb on + strand, within B158DRAFT_0587 at 592.473 kb on + strand, within B158DRAFT_0587 at 592.528 kb on + strand, within B158DRAFT_0587 at 592.660 kb on + strand at 592.660 kb on + strand at 592.668 kb on - strand at 592.755 kb on + strand, within B158DRAFT_0588 at 592.978 kb on + strand, within B158DRAFT_0588 at 592.986 kb on - strand, within B158DRAFT_0588
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.00390625 mg/ml remove 590,715 - +2.8 590,730 + +0.0 590,738 - +0.7 590,808 + -0.0 590,808 + -0.5 590,816 - +0.5 590,816 - +0.1 590,816 - -0.8 590,816 - +0.3 590,816 - +1.2 590,854 + +0.7 590,917 - -3.8 590,920 + -0.2 590,920 + +0.2 590,920 + -2.0 590,920 + -1.9 590,920 + -0.1 590,920 + -0.4 590,920 + +0.5 590,928 - +0.3 590,928 - -1.4 590,928 - -3.2 590,928 - -0.1 590,928 - -0.3 590,928 - -2.7 590,928 - -1.5 590,972 - B158DRAFT_0585 0.14 -0.8 591,017 + B158DRAFT_0585 0.19 -0.6 591,024 + B158DRAFT_0585 0.20 +0.9 591,078 + B158DRAFT_0585 0.27 +0.6 591,078 + B158DRAFT_0585 0.27 -0.2 591,078 + B158DRAFT_0585 0.27 +2.1 591,078 + B158DRAFT_0585 0.27 +1.0 591,086 - B158DRAFT_0585 0.28 -0.3 591,086 - B158DRAFT_0585 0.28 -0.0 591,086 - B158DRAFT_0585 0.28 +0.1 591,086 - B158DRAFT_0585 0.28 -3.4 591,086 - B158DRAFT_0585 0.28 +0.9 591,086 - B158DRAFT_0585 0.28 -0.4 591,086 - B158DRAFT_0585 0.28 +0.4 591,103 + B158DRAFT_0585 0.30 -0.1 591,132 + B158DRAFT_0585 0.34 +1.3 591,167 - B158DRAFT_0585 0.38 -1.2 591,190 + B158DRAFT_0585 0.41 -2.1 591,190 + B158DRAFT_0585 0.41 -3.7 591,198 - B158DRAFT_0585 0.42 -3.4 591,198 - B158DRAFT_0585 0.42 +0.5 591,398 + B158DRAFT_0585 0.68 -1.2 591,406 - B158DRAFT_0585 0.69 +0.5 591,406 - B158DRAFT_0585 0.69 +0.0 591,408 - B158DRAFT_0585 0.69 +0.3 591,495 + B158DRAFT_0585 0.80 +0.5 591,495 + B158DRAFT_0585 0.80 +1.6 591,502 + B158DRAFT_0585 0.81 -0.0 591,518 + B158DRAFT_0585 0.83 +1.1 591,518 + B158DRAFT_0585 0.83 -0.3 591,518 + B158DRAFT_0585 0.83 -0.5 591,518 + B158DRAFT_0585 0.83 +1.1 591,520 + B158DRAFT_0585 0.84 +1.1 591,526 - B158DRAFT_0585 0.84 +2.3 591,528 - B158DRAFT_0585 0.85 -0.1 591,528 - B158DRAFT_0585 0.85 +1.2 591,543 + B158DRAFT_0585 0.86 -4.2 591,609 + +0.4 591,609 + -0.0 591,609 + -5.6 591,609 + -0.2 591,609 + +1.3 591,609 + +1.5 591,609 + +0.0 591,609 + -4.7 591,609 + +0.5 591,609 + +0.2 591,609 + +0.6 591,609 + -1.1 591,609 + +0.2 591,609 + -0.4 591,609 + -0.3 591,609 + -0.9 591,609 + +0.1 591,609 + -0.2 591,609 + -0.8 591,609 + -0.5 591,617 - -1.7 591,617 - -0.8 591,617 - +0.4 591,617 - +0.3 591,617 - -1.0 591,617 - -3.4 591,617 - +2.9 591,617 - -0.7 591,617 - -0.6 591,617 - -0.5 591,617 - -1.0 591,617 - -0.1 591,617 - +0.3 591,617 - -0.6 591,617 - -1.6 591,617 - -0.5 591,651 - +0.6 591,651 - -0.4 591,651 - +0.4 591,653 + +0.5 591,661 - -0.4 591,733 - B158DRAFT_0586 0.14 -0.6 591,771 - B158DRAFT_0586 0.23 -0.1 591,888 - B158DRAFT_0586 0.50 -0.3 591,913 - B158DRAFT_0586 0.56 -2.8 591,929 - B158DRAFT_0586 0.59 +1.0 591,934 - B158DRAFT_0586 0.61 -1.1 591,934 - B158DRAFT_0586 0.61 -0.1 591,946 - B158DRAFT_0586 0.63 -4.1 592,004 + B158DRAFT_0586 0.77 -3.1 592,012 - B158DRAFT_0586 0.79 +0.3 592,052 + B158DRAFT_0586 0.88 +0.3 592,129 + +0.1 592,162 + B158DRAFT_0587 0.12 +0.5 592,162 + B158DRAFT_0587 0.12 +0.2 592,170 - B158DRAFT_0587 0.13 -0.6 592,178 + B158DRAFT_0587 0.15 -0.3 592,178 + B158DRAFT_0587 0.15 +1.2 592,275 + B158DRAFT_0587 0.35 -0.5 592,275 + B158DRAFT_0587 0.35 -0.1 592,275 + B158DRAFT_0587 0.35 -1.8 592,283 - B158DRAFT_0587 0.37 -0.9 592,334 + B158DRAFT_0587 0.47 +0.5 592,334 + B158DRAFT_0587 0.47 -0.5 592,342 - B158DRAFT_0587 0.49 -1.4 592,351 + B158DRAFT_0587 0.51 -0.2 592,374 + B158DRAFT_0587 0.56 -2.9 592,382 - B158DRAFT_0587 0.57 -0.2 592,382 - B158DRAFT_0587 0.57 -2.0 592,382 - B158DRAFT_0587 0.57 +1.1 592,389 - B158DRAFT_0587 0.59 +0.6 592,390 - B158DRAFT_0587 0.59 -4.6 592,421 + B158DRAFT_0587 0.65 -1.1 592,448 + B158DRAFT_0587 0.71 -0.9 592,473 + B158DRAFT_0587 0.76 +0.3 592,473 + B158DRAFT_0587 0.76 -0.6 592,473 + B158DRAFT_0587 0.76 +0.1 592,528 + B158DRAFT_0587 0.88 -0.1 592,660 + -0.4 592,660 + -4.3 592,668 - -0.2 592,755 + B158DRAFT_0588 0.12 -2.4 592,978 + B158DRAFT_0588 0.43 +0.1 592,986 - B158DRAFT_0588 0.44 -0.0
Or see this region's nucleotide sequence