Experiment: m.b. Polymyxin B sulfate 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0543 and B158DRAFT_0544 are separated by 130 nucleotides B158DRAFT_0544 and B158DRAFT_0545 are separated by 305 nucleotides B158DRAFT_0545 and B158DRAFT_0546 are separated by 68 nucleotides B158DRAFT_0546 and B158DRAFT_0547 are separated by 69 nucleotides
B158DRAFT_0543: B158DRAFT_0543 - glycine cleavage system T protein, at 548,728 to 549,816
_0543
B158DRAFT_0544: B158DRAFT_0544 - glycine cleavage system H protein, at 549,947 to 550,336
_0544
B158DRAFT_0545: B158DRAFT_0545 - glycine dehydrogenase (decarboxylating), at 550,642 to 553,527
_0545
B158DRAFT_0546: B158DRAFT_0546 - Tetrapyrrole (Corrin/Porphyrin) Methylases., at 553,596 to 554,396
_0546
B158DRAFT_0547: B158DRAFT_0547 - Aromatic-ring-opening dioxygenase LigAB, LigA subunit., at 554,466 to 554,687
_0547
Position (kb) 550
551
552
553
554 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 549.780 kb on + strand at 549.810 kb on + strand at 549.810 kb on + strand at 549.834 kb on - strand at 550.227 kb on - strand, within B158DRAFT_0544 at 550.324 kb on - strand at 553.529 kb on - strand at 553.619 kb on - strand at 553.650 kb on + strand at 553.698 kb on + strand, within B158DRAFT_0546 at 553.744 kb on + strand, within B158DRAFT_0546 at 553.752 kb on - strand, within B158DRAFT_0546 at 553.850 kb on + strand, within B158DRAFT_0546 at 553.858 kb on - strand, within B158DRAFT_0546 at 553.952 kb on + strand, within B158DRAFT_0546 at 554.022 kb on + strand, within B158DRAFT_0546 at 554.130 kb on - strand, within B158DRAFT_0546 at 554.152 kb on + strand, within B158DRAFT_0546 at 554.243 kb on + strand, within B158DRAFT_0546 at 554.251 kb on - strand, within B158DRAFT_0546 at 554.251 kb on - strand, within B158DRAFT_0546 at 554.321 kb on + strand at 554.396 kb on - strand at 554.526 kb on - strand, within B158DRAFT_0547
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.00390625 mg/ml remove 549,780 + +1.6 549,810 + +0.4 549,810 + -1.1 549,834 - -0.2 550,227 - B158DRAFT_0544 0.72 -2.1 550,324 - -2.4 553,529 - +0.7 553,619 - -0.5 553,650 + -1.7 553,698 + B158DRAFT_0546 0.13 -1.7 553,744 + B158DRAFT_0546 0.18 -3.7 553,752 - B158DRAFT_0546 0.19 -4.2 553,850 + B158DRAFT_0546 0.32 -1.2 553,858 - B158DRAFT_0546 0.33 +0.2 553,952 + B158DRAFT_0546 0.44 -1.7 554,022 + B158DRAFT_0546 0.53 -0.3 554,130 - B158DRAFT_0546 0.67 +0.2 554,152 + B158DRAFT_0546 0.69 -1.6 554,243 + B158DRAFT_0546 0.81 -1.6 554,251 - B158DRAFT_0546 0.82 -0.4 554,251 - B158DRAFT_0546 0.82 -4.8 554,321 + +0.9 554,396 - -0.0 554,526 - B158DRAFT_0547 0.27 -5.2
Or see this region's nucleotide sequence