Experiment: m.b. Polymyxin B sulfate 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0262 and B158DRAFT_0263 are separated by 98 nucleotides B158DRAFT_0263 and B158DRAFT_0264 are separated by 128 nucleotides B158DRAFT_0264 and B158DRAFT_0265 are separated by 365 nucleotides B158DRAFT_0265 and B158DRAFT_0266 are separated by 6 nucleotides
B158DRAFT_0262: B158DRAFT_0262 - hypothetical protein, at 268,544 to 269,149
_0262
B158DRAFT_0263: B158DRAFT_0263 - Predicted redox protein, regulator of disulfide bond formation, at 269,248 to 269,646
_0263
B158DRAFT_0264: B158DRAFT_0264 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 269,775 to 270,410
_0264
B158DRAFT_0265: B158DRAFT_0265 - Predicted endonuclease containing a URI domain, at 270,776 to 271,084
_0265
B158DRAFT_0266: B158DRAFT_0266 - glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase, at 271,091 to 271,951
_0266
Position (kb) 269
270
271 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 268.804 kb on - strand, within B158DRAFT_0262 at 268.829 kb on + strand, within B158DRAFT_0262 at 268.887 kb on + strand, within B158DRAFT_0262 at 268.887 kb on + strand, within B158DRAFT_0262 at 268.918 kb on + strand, within B158DRAFT_0262 at 268.918 kb on + strand, within B158DRAFT_0262 at 268.918 kb on + strand, within B158DRAFT_0262 at 268.926 kb on - strand, within B158DRAFT_0262 at 268.926 kb on - strand, within B158DRAFT_0262 at 268.926 kb on - strand, within B158DRAFT_0262 at 269.017 kb on + strand, within B158DRAFT_0262 at 269.022 kb on + strand, within B158DRAFT_0262 at 269.030 kb on - strand, within B158DRAFT_0262 at 269.030 kb on - strand, within B158DRAFT_0262 at 269.035 kb on - strand, within B158DRAFT_0262 at 269.187 kb on + strand at 269.187 kb on + strand at 269.325 kb on - strand, within B158DRAFT_0263 at 269.430 kb on - strand, within B158DRAFT_0263 at 269.528 kb on - strand, within B158DRAFT_0263 at 269.619 kb on + strand at 269.629 kb on + strand at 269.636 kb on - strand at 269.636 kb on - strand at 269.637 kb on - strand at 269.637 kb on - strand at 269.723 kb on - strand at 269.791 kb on - strand at 269.801 kb on + strand at 270.028 kb on - strand, within B158DRAFT_0264 at 270.030 kb on + strand, within B158DRAFT_0264 at 270.030 kb on + strand, within B158DRAFT_0264 at 270.038 kb on - strand, within B158DRAFT_0264 at 270.150 kb on - strand, within B158DRAFT_0264 at 270.259 kb on - strand, within B158DRAFT_0264 at 270.264 kb on - strand, within B158DRAFT_0264 at 270.272 kb on + strand, within B158DRAFT_0264 at 270.272 kb on + strand, within B158DRAFT_0264 at 270.272 kb on + strand, within B158DRAFT_0264 at 270.280 kb on - strand, within B158DRAFT_0264 at 270.280 kb on - strand, within B158DRAFT_0264 at 270.334 kb on - strand, within B158DRAFT_0264 at 270.676 kb on + strand at 270.676 kb on + strand at 270.684 kb on - strand at 270.684 kb on - strand at 270.712 kb on - strand at 270.828 kb on + strand, within B158DRAFT_0265 at 270.828 kb on + strand, within B158DRAFT_0265 at 270.836 kb on - strand, within B158DRAFT_0265 at 270.893 kb on + strand, within B158DRAFT_0265 at 271.071 kb on - strand at 271.079 kb on + strand at 271.089 kb on + strand at 271.203 kb on + strand, within B158DRAFT_0266 at 271.251 kb on + strand, within B158DRAFT_0266 at 271.259 kb on + strand, within B158DRAFT_0266 at 271.259 kb on - strand, within B158DRAFT_0266 at 271.330 kb on - strand, within B158DRAFT_0266 at 271.368 kb on - strand, within B158DRAFT_0266 at 271.395 kb on - strand, within B158DRAFT_0266
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.00390625 mg/ml remove 268,804 - B158DRAFT_0262 0.43 -2.0 268,829 + B158DRAFT_0262 0.47 -0.7 268,887 + B158DRAFT_0262 0.57 +0.5 268,887 + B158DRAFT_0262 0.57 -2.2 268,918 + B158DRAFT_0262 0.62 +0.1 268,918 + B158DRAFT_0262 0.62 +0.0 268,918 + B158DRAFT_0262 0.62 +0.7 268,926 - B158DRAFT_0262 0.63 -0.1 268,926 - B158DRAFT_0262 0.63 +0.1 268,926 - B158DRAFT_0262 0.63 -2.8 269,017 + B158DRAFT_0262 0.78 -0.1 269,022 + B158DRAFT_0262 0.79 -0.4 269,030 - B158DRAFT_0262 0.80 +0.1 269,030 - B158DRAFT_0262 0.80 -3.3 269,035 - B158DRAFT_0262 0.81 +1.9 269,187 + -0.0 269,187 + +0.2 269,325 - B158DRAFT_0263 0.19 -2.9 269,430 - B158DRAFT_0263 0.46 -1.3 269,528 - B158DRAFT_0263 0.70 -4.3 269,619 + -0.1 269,629 + -0.7 269,636 - +0.0 269,636 - -0.3 269,637 - +2.8 269,637 - -2.0 269,723 - +0.7 269,791 - +0.5 269,801 + -2.4 270,028 - B158DRAFT_0264 0.40 +0.6 270,030 + B158DRAFT_0264 0.40 -0.5 270,030 + B158DRAFT_0264 0.40 -0.2 270,038 - B158DRAFT_0264 0.41 -3.2 270,150 - B158DRAFT_0264 0.59 -0.8 270,259 - B158DRAFT_0264 0.76 -1.0 270,264 - B158DRAFT_0264 0.77 -1.7 270,272 + B158DRAFT_0264 0.78 +1.0 270,272 + B158DRAFT_0264 0.78 -0.2 270,272 + B158DRAFT_0264 0.78 -3.1 270,280 - B158DRAFT_0264 0.79 +0.2 270,280 - B158DRAFT_0264 0.79 -4.2 270,334 - B158DRAFT_0264 0.88 -0.5 270,676 + +0.0 270,676 + -0.2 270,684 - -0.5 270,684 - -2.2 270,712 - -0.5 270,828 + B158DRAFT_0265 0.17 -3.8 270,828 + B158DRAFT_0265 0.17 +0.3 270,836 - B158DRAFT_0265 0.19 -1.4 270,893 + B158DRAFT_0265 0.38 +0.7 271,071 - +0.7 271,079 + -0.4 271,089 + -0.6 271,203 + B158DRAFT_0266 0.13 +0.7 271,251 + B158DRAFT_0266 0.19 -0.5 271,259 + B158DRAFT_0266 0.20 -0.9 271,259 - B158DRAFT_0266 0.20 -1.1 271,330 - B158DRAFT_0266 0.28 +1.0 271,368 - B158DRAFT_0266 0.32 +0.7 271,395 - B158DRAFT_0266 0.35 -2.3
Or see this region's nucleotide sequence