Experiment: m.b. copper (II) chloride 0.625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2022 and B158DRAFT_2023 are separated by 99 nucleotides B158DRAFT_2023 and B158DRAFT_2024 are separated by 69 nucleotides B158DRAFT_2024 and B158DRAFT_2025 are separated by 76 nucleotides
B158DRAFT_2022: B158DRAFT_2022 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 151,723 to 153,150
_2022
B158DRAFT_2023: B158DRAFT_2023 - Zn-dependent hydrolases, including glyoxylases, at 153,250 to 154,173
_2023
B158DRAFT_2024: B158DRAFT_2024 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 154,243 to 154,977
_2024
B158DRAFT_2025: B158DRAFT_2025 - Uncharacterized protein conserved in bacteria, at 155,054 to 155,488
_2025
Position (kb)
153
154
155 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.407 kb on + strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.415 kb on - strand, within B158DRAFT_2022 at 152.450 kb on - strand, within B158DRAFT_2022 at 152.455 kb on + strand, within B158DRAFT_2022 at 152.455 kb on + strand, within B158DRAFT_2022 at 152.458 kb on - strand, within B158DRAFT_2022 at 152.463 kb on - strand, within B158DRAFT_2022 at 152.467 kb on + strand, within B158DRAFT_2022 at 152.523 kb on + strand, within B158DRAFT_2022 at 152.523 kb on + strand, within B158DRAFT_2022 at 152.531 kb on - strand, within B158DRAFT_2022 at 152.531 kb on - strand, within B158DRAFT_2022 at 152.544 kb on + strand, within B158DRAFT_2022 at 152.573 kb on - strand, within B158DRAFT_2022 at 152.590 kb on - strand, within B158DRAFT_2022 at 152.624 kb on - strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.631 kb on + strand, within B158DRAFT_2022 at 152.639 kb on - strand, within B158DRAFT_2022 at 152.641 kb on + strand, within B158DRAFT_2022 at 152.646 kb on + strand, within B158DRAFT_2022 at 152.664 kb on - strand, within B158DRAFT_2022 at 152.686 kb on + strand, within B158DRAFT_2022 at 152.728 kb on + strand, within B158DRAFT_2022 at 152.729 kb on + strand, within B158DRAFT_2022 at 152.736 kb on - strand, within B158DRAFT_2022 at 152.738 kb on + strand, within B158DRAFT_2022 at 152.810 kb on - strand, within B158DRAFT_2022 at 152.865 kb on - strand, within B158DRAFT_2022 at 152.865 kb on - strand, within B158DRAFT_2022 at 152.892 kb on - strand, within B158DRAFT_2022 at 152.971 kb on + strand, within B158DRAFT_2022 at 152.971 kb on + strand, within B158DRAFT_2022 at 152.971 kb on + strand, within B158DRAFT_2022 at 152.971 kb on + strand, within B158DRAFT_2022 at 152.971 kb on + strand, within B158DRAFT_2022 at 152.971 kb on + strand, within B158DRAFT_2022 at 152.971 kb on + strand, within B158DRAFT_2022 at 152.979 kb on - strand, within B158DRAFT_2022 at 152.979 kb on - strand, within B158DRAFT_2022 at 152.979 kb on - strand, within B158DRAFT_2022 at 153.012 kb on + strand at 153.022 kb on + strand at 153.027 kb on + strand at 153.127 kb on + strand at 153.175 kb on - strand at 153.211 kb on + strand at 153.315 kb on - strand at 153.326 kb on - strand at 153.336 kb on - strand at 153.350 kb on + strand, within B158DRAFT_2023 at 153.353 kb on - strand, within B158DRAFT_2023 at 153.357 kb on - strand, within B158DRAFT_2023 at 153.376 kb on + strand, within B158DRAFT_2023 at 153.381 kb on + strand, within B158DRAFT_2023 at 153.435 kb on + strand, within B158DRAFT_2023 at 153.480 kb on - strand, within B158DRAFT_2023 at 153.613 kb on - strand, within B158DRAFT_2023 at 153.709 kb on + strand, within B158DRAFT_2023 at 153.734 kb on + strand, within B158DRAFT_2023 at 153.742 kb on - strand, within B158DRAFT_2023 at 153.742 kb on - strand, within B158DRAFT_2023 at 153.780 kb on + strand, within B158DRAFT_2023 at 153.782 kb on - strand, within B158DRAFT_2023 at 153.788 kb on - strand, within B158DRAFT_2023 at 153.788 kb on - strand, within B158DRAFT_2023 at 153.826 kb on + strand, within B158DRAFT_2023 at 153.831 kb on + strand, within B158DRAFT_2023 at 153.834 kb on - strand, within B158DRAFT_2023 at 153.874 kb on + strand, within B158DRAFT_2023 at 153.903 kb on - strand, within B158DRAFT_2023 at 153.973 kb on + strand, within B158DRAFT_2023 at 154.168 kb on + strand at 154.177 kb on + strand at 154.185 kb on - strand at 154.273 kb on + strand at 154.315 kb on + strand at 154.322 kb on - strand, within B158DRAFT_2024 at 154.322 kb on - strand, within B158DRAFT_2024 at 154.322 kb on - strand, within B158DRAFT_2024 at 154.323 kb on + strand, within B158DRAFT_2024 at 154.545 kb on + strand, within B158DRAFT_2024 at 154.597 kb on - strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.605 kb on + strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.640 kb on - strand, within B158DRAFT_2024 at 154.649 kb on - strand, within B158DRAFT_2024 at 154.733 kb on - strand, within B158DRAFT_2024 at 154.748 kb on + strand, within B158DRAFT_2024 at 154.784 kb on + strand, within B158DRAFT_2024 at 154.794 kb on + strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.803 kb on - strand, within B158DRAFT_2024 at 154.813 kb on + strand, within B158DRAFT_2024 at 154.813 kb on + strand, within B158DRAFT_2024 at 154.821 kb on - strand, within B158DRAFT_2024 at 154.828 kb on + strand, within B158DRAFT_2024 at 154.831 kb on - strand, within B158DRAFT_2024 at 154.841 kb on + strand, within B158DRAFT_2024 at 154.932 kb on + strand at 155.000 kb on - strand at 155.010 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. copper (II) chloride 0.625 mM remove 152,407 + B158DRAFT_2022 0.48 -1.1 152,407 + B158DRAFT_2022 0.48 +0.4 152,407 + B158DRAFT_2022 0.48 -0.2 152,407 + B158DRAFT_2022 0.48 -1.8 152,407 + B158DRAFT_2022 0.48 -0.9 152,407 + B158DRAFT_2022 0.48 +2.7 152,407 + B158DRAFT_2022 0.48 -0.6 152,407 + B158DRAFT_2022 0.48 +0.3 152,407 + B158DRAFT_2022 0.48 -0.9 152,407 + B158DRAFT_2022 0.48 +0.2 152,407 + B158DRAFT_2022 0.48 +1.3 152,407 + B158DRAFT_2022 0.48 -0.8 152,407 + B158DRAFT_2022 0.48 -0.4 152,407 + B158DRAFT_2022 0.48 -0.5 152,415 - B158DRAFT_2022 0.48 -1.2 152,415 - B158DRAFT_2022 0.48 -0.2 152,415 - B158DRAFT_2022 0.48 -1.4 152,415 - B158DRAFT_2022 0.48 +3.1 152,415 - B158DRAFT_2022 0.48 -2.8 152,415 - B158DRAFT_2022 0.48 +1.0 152,415 - B158DRAFT_2022 0.48 -0.6 152,415 - B158DRAFT_2022 0.48 -0.2 152,415 - B158DRAFT_2022 0.48 -1.2 152,415 - B158DRAFT_2022 0.48 -1.9 152,415 - B158DRAFT_2022 0.48 -0.3 152,415 - B158DRAFT_2022 0.48 -0.3 152,415 - B158DRAFT_2022 0.48 -0.1 152,450 - B158DRAFT_2022 0.51 +0.3 152,455 + B158DRAFT_2022 0.51 -0.1 152,455 + B158DRAFT_2022 0.51 +0.6 152,458 - B158DRAFT_2022 0.51 +0.0 152,463 - B158DRAFT_2022 0.52 -0.1 152,467 + B158DRAFT_2022 0.52 -0.1 152,523 + B158DRAFT_2022 0.56 -0.8 152,523 + B158DRAFT_2022 0.56 +0.5 152,531 - B158DRAFT_2022 0.57 +1.7 152,531 - B158DRAFT_2022 0.57 +0.4 152,544 + B158DRAFT_2022 0.57 +0.8 152,573 - B158DRAFT_2022 0.60 -0.8 152,590 - B158DRAFT_2022 0.61 -1.3 152,624 - B158DRAFT_2022 0.63 -0.7 152,631 + B158DRAFT_2022 0.64 -0.5 152,631 + B158DRAFT_2022 0.64 -0.0 152,631 + B158DRAFT_2022 0.64 -1.2 152,631 + B158DRAFT_2022 0.64 -1.3 152,631 + B158DRAFT_2022 0.64 -0.4 152,631 + B158DRAFT_2022 0.64 -1.3 152,631 + B158DRAFT_2022 0.64 -0.9 152,631 + B158DRAFT_2022 0.64 -0.7 152,631 + B158DRAFT_2022 0.64 -0.8 152,631 + B158DRAFT_2022 0.64 -0.3 152,631 + B158DRAFT_2022 0.64 -0.3 152,631 + B158DRAFT_2022 0.64 -0.4 152,631 + B158DRAFT_2022 0.64 -0.1 152,631 + B158DRAFT_2022 0.64 -0.5 152,639 - B158DRAFT_2022 0.64 -0.3 152,641 + B158DRAFT_2022 0.64 -0.1 152,646 + B158DRAFT_2022 0.65 -0.6 152,664 - B158DRAFT_2022 0.66 -1.3 152,686 + B158DRAFT_2022 0.67 -1.0 152,728 + B158DRAFT_2022 0.70 -0.0 152,729 + B158DRAFT_2022 0.70 -1.1 152,736 - B158DRAFT_2022 0.71 -1.2 152,738 + B158DRAFT_2022 0.71 -1.8 152,810 - B158DRAFT_2022 0.76 -2.5 152,865 - B158DRAFT_2022 0.80 -1.5 152,865 - B158DRAFT_2022 0.80 -0.3 152,892 - B158DRAFT_2022 0.82 -1.3 152,971 + B158DRAFT_2022 0.87 +6.4 152,971 + B158DRAFT_2022 0.87 -0.8 152,971 + B158DRAFT_2022 0.87 -2.5 152,971 + B158DRAFT_2022 0.87 +0.2 152,971 + B158DRAFT_2022 0.87 -1.3 152,971 + B158DRAFT_2022 0.87 -1.4 152,971 + B158DRAFT_2022 0.87 -0.7 152,979 - B158DRAFT_2022 0.88 +0.8 152,979 - B158DRAFT_2022 0.88 +1.1 152,979 - B158DRAFT_2022 0.88 -1.9 153,012 + -1.2 153,022 + -0.7 153,027 + -2.3 153,127 + +2.3 153,175 - +0.8 153,211 + -0.8 153,315 - +2.4 153,326 - -0.8 153,336 - +1.6 153,350 + B158DRAFT_2023 0.11 -0.7 153,353 - B158DRAFT_2023 0.11 -1.0 153,357 - B158DRAFT_2023 0.12 -0.3 153,376 + B158DRAFT_2023 0.14 +0.1 153,381 + B158DRAFT_2023 0.14 -1.2 153,435 + B158DRAFT_2023 0.20 -0.4 153,480 - B158DRAFT_2023 0.25 +0.1 153,613 - B158DRAFT_2023 0.39 -1.9 153,709 + B158DRAFT_2023 0.50 -0.0 153,734 + B158DRAFT_2023 0.52 -0.9 153,742 - B158DRAFT_2023 0.53 +0.2 153,742 - B158DRAFT_2023 0.53 +0.4 153,780 + B158DRAFT_2023 0.57 -2.8 153,782 - B158DRAFT_2023 0.58 -0.5 153,788 - B158DRAFT_2023 0.58 -0.4 153,788 - B158DRAFT_2023 0.58 +0.4 153,826 + B158DRAFT_2023 0.62 -0.3 153,831 + B158DRAFT_2023 0.63 -3.0 153,834 - B158DRAFT_2023 0.63 +0.3 153,874 + B158DRAFT_2023 0.68 -0.9 153,903 - B158DRAFT_2023 0.71 -0.7 153,973 + B158DRAFT_2023 0.78 -1.2 154,168 + -1.4 154,177 + -0.6 154,185 - -0.5 154,273 + +1.8 154,315 + +0.2 154,322 - B158DRAFT_2024 0.11 +0.6 154,322 - B158DRAFT_2024 0.11 +0.7 154,322 - B158DRAFT_2024 0.11 -1.0 154,323 + B158DRAFT_2024 0.11 -0.4 154,545 + B158DRAFT_2024 0.41 -2.7 154,597 - B158DRAFT_2024 0.48 -0.9 154,599 + B158DRAFT_2024 0.48 -0.3 154,599 + B158DRAFT_2024 0.48 +1.3 154,599 + B158DRAFT_2024 0.48 -1.0 154,599 + B158DRAFT_2024 0.48 +1.5 154,599 + B158DRAFT_2024 0.48 -1.0 154,599 + B158DRAFT_2024 0.48 -0.9 154,605 + B158DRAFT_2024 0.49 +0.2 154,607 - B158DRAFT_2024 0.50 +0.7 154,607 - B158DRAFT_2024 0.50 -1.2 154,607 - B158DRAFT_2024 0.50 -0.1 154,640 - B158DRAFT_2024 0.54 +0.4 154,649 - B158DRAFT_2024 0.55 +0.4 154,733 - B158DRAFT_2024 0.67 +3.4 154,748 + B158DRAFT_2024 0.69 +1.0 154,784 + B158DRAFT_2024 0.74 +0.3 154,794 + B158DRAFT_2024 0.75 -0.7 154,802 - B158DRAFT_2024 0.76 +0.4 154,802 - B158DRAFT_2024 0.76 -1.1 154,802 - B158DRAFT_2024 0.76 +0.6 154,803 - B158DRAFT_2024 0.76 +0.6 154,813 + B158DRAFT_2024 0.78 +0.6 154,813 + B158DRAFT_2024 0.78 +0.4 154,821 - B158DRAFT_2024 0.79 -0.4 154,828 + B158DRAFT_2024 0.80 +3.3 154,831 - B158DRAFT_2024 0.80 +0.2 154,841 + B158DRAFT_2024 0.81 +1.5 154,932 + -2.0 155,000 - -0.3 155,010 + +2.2
Or see this region's nucleotide sequence