Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2428 and B158DRAFT_2429 overlap by 4 nucleotides B158DRAFT_2429 and B158DRAFT_2430 are separated by 25 nucleotides B158DRAFT_2430 and B158DRAFT_2431 overlap by 56 nucleotides
B158DRAFT_2428: B158DRAFT_2428 - indole-3-glycerol phosphate synthase (EC 4.1.1.48), at 600,950 to 602,410
_2428
B158DRAFT_2429: B158DRAFT_2429 - tryptophan synthase, beta subunit, at 602,407 to 603,618
_2429
B158DRAFT_2430: B158DRAFT_2430 - tryptophan synthase, alpha chain (EC 4.2.1.20), at 603,644 to 604,549
_2430
B158DRAFT_2431: B158DRAFT_2431 - hypothetical protein, at 604,494 to 604,742
_2431
Position (kb) 602
603
604 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9 at 601.417 kb on + strand, within B158DRAFT_2428 at 601.425 kb on - strand, within B158DRAFT_2428 at 601.490 kb on + strand, within B158DRAFT_2428 at 601.509 kb on + strand, within B158DRAFT_2428 at 601.943 kb on - strand, within B158DRAFT_2428 at 601.943 kb on - strand, within B158DRAFT_2428 at 601.966 kb on + strand, within B158DRAFT_2428 at 602.133 kb on + strand, within B158DRAFT_2428 at 602.360 kb on + strand at 602.368 kb on - strand at 602.374 kb on + strand at 602.374 kb on + strand at 602.770 kb on + strand, within B158DRAFT_2429 at 602.770 kb on + strand, within B158DRAFT_2429 at 602.791 kb on + strand, within B158DRAFT_2429 at 602.791 kb on + strand, within B158DRAFT_2429 at 602.799 kb on - strand, within B158DRAFT_2429 at 602.799 kb on - strand, within B158DRAFT_2429 at 602.861 kb on + strand, within B158DRAFT_2429 at 602.994 kb on + strand, within B158DRAFT_2429 at 603.180 kb on + strand, within B158DRAFT_2429 at 603.188 kb on - strand, within B158DRAFT_2429 at 603.283 kb on - strand, within B158DRAFT_2429 at 603.297 kb on + strand, within B158DRAFT_2429 at 603.348 kb on - strand, within B158DRAFT_2429 at 603.348 kb on - strand, within B158DRAFT_2429 at 603.372 kb on + strand, within B158DRAFT_2429 at 603.590 kb on - strand at 604.091 kb on + strand, within B158DRAFT_2430 at 604.161 kb on - strand, within B158DRAFT_2430 at 604.161 kb on - strand, within B158DRAFT_2430 at 604.161 kb on - strand, within B158DRAFT_2430 at 604.161 kb on - strand, within B158DRAFT_2430 at 604.327 kb on - strand, within B158DRAFT_2430
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 601,417 + B158DRAFT_2428 0.32 +0.1 601,425 - B158DRAFT_2428 0.33 +2.3 601,490 + B158DRAFT_2428 0.37 +0.0 601,509 + B158DRAFT_2428 0.38 -1.9 601,943 - B158DRAFT_2428 0.68 +0.7 601,943 - B158DRAFT_2428 0.68 +1.7 601,966 + B158DRAFT_2428 0.70 +2.4 602,133 + B158DRAFT_2428 0.81 -1.1 602,360 + -1.6 602,368 - +1.2 602,374 + +0.1 602,374 + -2.2 602,770 + B158DRAFT_2429 0.30 -1.0 602,770 + B158DRAFT_2429 0.30 -1.8 602,791 + B158DRAFT_2429 0.32 -0.1 602,791 + B158DRAFT_2429 0.32 -0.1 602,799 - B158DRAFT_2429 0.32 +0.6 602,799 - B158DRAFT_2429 0.32 -1.1 602,861 + B158DRAFT_2429 0.37 -0.8 602,994 + B158DRAFT_2429 0.48 +0.0 603,180 + B158DRAFT_2429 0.64 -1.4 603,188 - B158DRAFT_2429 0.64 -0.3 603,283 - B158DRAFT_2429 0.72 +2.0 603,297 + B158DRAFT_2429 0.73 -1.0 603,348 - B158DRAFT_2429 0.78 -0.7 603,348 - B158DRAFT_2429 0.78 +0.5 603,372 + B158DRAFT_2429 0.80 +0.3 603,590 - -0.5 604,091 + B158DRAFT_2430 0.49 -1.0 604,161 - B158DRAFT_2430 0.57 +0.9 604,161 - B158DRAFT_2430 0.57 +0.4 604,161 - B158DRAFT_2430 0.57 +2.1 604,161 - B158DRAFT_2430 0.57 -1.6 604,327 - B158DRAFT_2430 0.75 +8.8
Or see this region's nucleotide sequence