Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2265 and B158DRAFT_2266 are separated by 145 nucleotides B158DRAFT_2266 and B158DRAFT_2267 are separated by 97 nucleotides B158DRAFT_2267 and B158DRAFT_2268 are separated by 92 nucleotides
B158DRAFT_2265: B158DRAFT_2265 - tyrosine recombinase XerD, at 427,701 to 428,588
_2265
B158DRAFT_2266: B158DRAFT_2266 - Protein-disulfide isomerase, at 428,734 to 429,492
_2266
B158DRAFT_2267: B158DRAFT_2267 - Uncharacterized conserved protein, at 429,590 to 429,958
_2267
B158DRAFT_2268: B158DRAFT_2268 - single-stranded-DNA-specific exonuclease RecJ, at 430,051 to 431,778
_2268
Position (kb) 428
429
430 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 427.736 kb on + strand at 427.744 kb on - strand at 427.744 kb on - strand at 427.744 kb on - strand at 427.754 kb on - strand at 427.759 kb on - strand at 427.759 kb on - strand at 427.989 kb on - strand, within B158DRAFT_2265 at 428.053 kb on - strand, within B158DRAFT_2265 at 428.082 kb on + strand, within B158DRAFT_2265 at 428.138 kb on - strand, within B158DRAFT_2265 at 428.187 kb on + strand, within B158DRAFT_2265 at 428.187 kb on + strand, within B158DRAFT_2265 at 428.210 kb on + strand, within B158DRAFT_2265 at 428.218 kb on - strand, within B158DRAFT_2265 at 428.218 kb on - strand, within B158DRAFT_2265 at 428.232 kb on + strand, within B158DRAFT_2265 at 428.239 kb on - strand, within B158DRAFT_2265 at 428.262 kb on + strand, within B158DRAFT_2265 at 428.263 kb on - strand, within B158DRAFT_2265 at 428.265 kb on - strand, within B158DRAFT_2265 at 428.275 kb on - strand, within B158DRAFT_2265 at 428.277 kb on - strand, within B158DRAFT_2265 at 428.282 kb on - strand, within B158DRAFT_2265 at 428.289 kb on + strand, within B158DRAFT_2265 at 428.329 kb on + strand, within B158DRAFT_2265 at 428.352 kb on + strand, within B158DRAFT_2265 at 428.365 kb on - strand, within B158DRAFT_2265 at 428.418 kb on + strand, within B158DRAFT_2265 at 428.426 kb on - strand, within B158DRAFT_2265 at 428.453 kb on + strand, within B158DRAFT_2265 at 428.453 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.471 kb on + strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.479 kb on - strand, within B158DRAFT_2265 at 428.490 kb on - strand, within B158DRAFT_2265 at 428.522 kb on + strand at 428.522 kb on + strand at 428.522 kb on + strand at 428.522 kb on + strand at 428.522 kb on + strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.530 kb on - strand at 428.556 kb on + strand at 428.607 kb on + strand at 428.801 kb on - strand at 428.868 kb on + strand, within B158DRAFT_2266 at 428.889 kb on + strand, within B158DRAFT_2266 at 429.070 kb on + strand, within B158DRAFT_2266 at 429.234 kb on - strand, within B158DRAFT_2266 at 429.252 kb on + strand, within B158DRAFT_2266 at 429.267 kb on - strand, within B158DRAFT_2266 at 429.352 kb on + strand, within B158DRAFT_2266 at 429.469 kb on - strand at 429.479 kb on - strand at 429.505 kb on - strand at 429.516 kb on - strand at 429.535 kb on + strand at 429.538 kb on - strand at 429.594 kb on + strand at 429.594 kb on + strand at 429.596 kb on + strand at 429.597 kb on - strand at 429.600 kb on + strand at 429.602 kb on - strand at 429.607 kb on - strand at 429.613 kb on - strand at 429.618 kb on - strand at 429.639 kb on + strand, within B158DRAFT_2267 at 429.663 kb on - strand, within B158DRAFT_2267 at 429.671 kb on - strand, within B158DRAFT_2267 at 429.700 kb on - strand, within B158DRAFT_2267 at 429.733 kb on + strand, within B158DRAFT_2267 at 429.784 kb on + strand, within B158DRAFT_2267 at 429.784 kb on - strand, within B158DRAFT_2267 at 429.834 kb on + strand, within B158DRAFT_2267 at 429.924 kb on - strand at 429.935 kb on + strand at 429.936 kb on + strand at 429.936 kb on + strand at 429.944 kb on - strand at 429.944 kb on - strand at 429.944 kb on - strand at 429.944 kb on - strand at 429.974 kb on + strand at 429.974 kb on + strand at 429.974 kb on + strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 429.982 kb on - strand at 430.099 kb on + strand at 430.103 kb on + strand at 430.133 kb on - strand at 430.133 kb on - strand at 430.133 kb on - strand at 430.142 kb on + strand at 430.142 kb on + strand at 430.142 kb on + strand at 430.150 kb on - strand at 430.150 kb on - strand at 430.150 kb on - strand at 430.159 kb on + strand at 430.219 kb on + strand at 430.389 kb on - strand, within B158DRAFT_2268 at 430.395 kb on - strand, within B158DRAFT_2268 at 430.422 kb on + strand, within B158DRAFT_2268 at 430.476 kb on + strand, within B158DRAFT_2268 at 430.476 kb on + strand, within B158DRAFT_2268 at 430.476 kb on + strand, within B158DRAFT_2268 at 430.484 kb on - strand, within B158DRAFT_2268
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 427,736 + -1.1 427,744 - -0.3 427,744 - -1.0 427,744 - -0.7 427,754 - +0.1 427,759 - -0.4 427,759 - -1.4 427,989 - B158DRAFT_2265 0.32 -0.9 428,053 - B158DRAFT_2265 0.40 -0.9 428,082 + B158DRAFT_2265 0.43 -0.2 428,138 - B158DRAFT_2265 0.49 -0.9 428,187 + B158DRAFT_2265 0.55 -1.2 428,187 + B158DRAFT_2265 0.55 +0.5 428,210 + B158DRAFT_2265 0.57 -0.5 428,218 - B158DRAFT_2265 0.58 -1.3 428,218 - B158DRAFT_2265 0.58 -1.9 428,232 + B158DRAFT_2265 0.60 -1.2 428,239 - B158DRAFT_2265 0.61 -1.1 428,262 + B158DRAFT_2265 0.63 -1.5 428,263 - B158DRAFT_2265 0.63 -3.1 428,265 - B158DRAFT_2265 0.64 +3.8 428,275 - B158DRAFT_2265 0.65 -0.1 428,277 - B158DRAFT_2265 0.65 -0.9 428,282 - B158DRAFT_2265 0.65 -1.2 428,289 + B158DRAFT_2265 0.66 -1.9 428,329 + B158DRAFT_2265 0.71 +0.6 428,352 + B158DRAFT_2265 0.73 -2.2 428,365 - B158DRAFT_2265 0.75 +0.4 428,418 + B158DRAFT_2265 0.81 -0.5 428,426 - B158DRAFT_2265 0.82 -0.8 428,453 + B158DRAFT_2265 0.85 -0.5 428,453 + B158DRAFT_2265 0.85 -2.4 428,471 + B158DRAFT_2265 0.87 -2.6 428,471 + B158DRAFT_2265 0.87 -0.4 428,471 + B158DRAFT_2265 0.87 -0.6 428,471 + B158DRAFT_2265 0.87 -1.3 428,471 + B158DRAFT_2265 0.87 +0.2 428,479 - B158DRAFT_2265 0.88 -0.9 428,479 - B158DRAFT_2265 0.88 +0.2 428,479 - B158DRAFT_2265 0.88 -0.4 428,479 - B158DRAFT_2265 0.88 -0.2 428,479 - B158DRAFT_2265 0.88 -0.1 428,490 - B158DRAFT_2265 0.89 -0.8 428,522 + +0.5 428,522 + -0.9 428,522 + -1.2 428,522 + -1.2 428,522 + -2.0 428,530 - -0.1 428,530 - -0.2 428,530 - +1.1 428,530 - -1.0 428,530 - -2.4 428,530 - -1.9 428,530 - +0.2 428,556 + -0.5 428,607 + -1.2 428,801 - -1.4 428,868 + B158DRAFT_2266 0.18 -1.0 428,889 + B158DRAFT_2266 0.20 +1.1 429,070 + B158DRAFT_2266 0.44 -1.0 429,234 - B158DRAFT_2266 0.66 -0.1 429,252 + B158DRAFT_2266 0.68 +2.1 429,267 - B158DRAFT_2266 0.70 +0.8 429,352 + B158DRAFT_2266 0.81 -0.2 429,469 - -0.4 429,479 - +4.1 429,505 - -1.5 429,516 - -0.6 429,535 + -1.4 429,538 - -0.3 429,594 + -0.1 429,594 + -0.1 429,596 + +1.4 429,597 - -0.7 429,600 + +1.1 429,602 - +1.3 429,607 - +0.4 429,613 - +0.9 429,618 - +0.4 429,639 + B158DRAFT_2267 0.13 +2.6 429,663 - B158DRAFT_2267 0.20 +1.5 429,671 - B158DRAFT_2267 0.22 +1.8 429,700 - B158DRAFT_2267 0.30 -0.5 429,733 + B158DRAFT_2267 0.39 +1.1 429,784 + B158DRAFT_2267 0.53 +1.1 429,784 - B158DRAFT_2267 0.53 +1.2 429,834 + B158DRAFT_2267 0.66 +1.3 429,924 - +3.1 429,935 + +4.8 429,936 + +1.8 429,936 + -0.2 429,944 - -0.1 429,944 - +2.3 429,944 - -0.1 429,944 - -0.4 429,974 + +0.8 429,974 + -2.1 429,974 + +1.5 429,982 - -1.2 429,982 - +1.9 429,982 - -0.7 429,982 - +1.5 429,982 - -0.8 429,982 - -0.8 430,099 + +0.7 430,103 + +0.3 430,133 - -0.3 430,133 - -0.3 430,133 - +0.6 430,142 + +1.8 430,142 + +2.0 430,142 + +0.3 430,150 - -0.0 430,150 - -0.9 430,150 - -0.5 430,159 + +2.6 430,219 + +0.6 430,389 - B158DRAFT_2268 0.20 -0.3 430,395 - B158DRAFT_2268 0.20 -0.5 430,422 + B158DRAFT_2268 0.21 -0.8 430,476 + B158DRAFT_2268 0.25 +0.5 430,476 + B158DRAFT_2268 0.25 -1.4 430,476 + B158DRAFT_2268 0.25 +1.7 430,484 - B158DRAFT_2268 0.25 -0.6
Or see this region's nucleotide sequence