Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2050 and B158DRAFT_2051 are separated by 126 nucleotides B158DRAFT_2051 and B158DRAFT_2052 are separated by 98 nucleotides
B158DRAFT_2050: B158DRAFT_2050 - hypothetical protein, at 183,675 to 185,612
_2050
B158DRAFT_2051: B158DRAFT_2051 - tRNA_Met_CAT, at 185,739 to 185,815
_2051
B158DRAFT_2052: B158DRAFT_2052 - GTP-binding protein YchF, at 185,914 to 187,005
_2052
Position (kb)
185
186 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 184.784 kb on + strand, within B158DRAFT_2050 at 184.784 kb on + strand, within B158DRAFT_2050 at 184.789 kb on + strand, within B158DRAFT_2050 at 184.982 kb on + strand, within B158DRAFT_2050 at 185.447 kb on - strand at 185.547 kb on - strand at 185.548 kb on + strand at 185.555 kb on + strand at 185.556 kb on - strand at 185.911 kb on + strand at 185.919 kb on - strand at 185.919 kb on - strand at 185.940 kb on - strand at 185.957 kb on + strand at 185.965 kb on - strand at 185.965 kb on - strand at 186.055 kb on + strand, within B158DRAFT_2052 at 186.055 kb on + strand, within B158DRAFT_2052 at 186.063 kb on - strand, within B158DRAFT_2052 at 186.076 kb on + strand, within B158DRAFT_2052 at 186.178 kb on - strand, within B158DRAFT_2052 at 186.179 kb on - strand, within B158DRAFT_2052 at 186.181 kb on + strand, within B158DRAFT_2052 at 186.181 kb on + strand, within B158DRAFT_2052 at 186.181 kb on + strand, within B158DRAFT_2052 at 186.181 kb on + strand, within B158DRAFT_2052 at 186.189 kb on - strand, within B158DRAFT_2052 at 186.189 kb on - strand, within B158DRAFT_2052 at 186.189 kb on - strand, within B158DRAFT_2052 at 186.189 kb on - strand, within B158DRAFT_2052 at 186.189 kb on - strand, within B158DRAFT_2052 at 186.205 kb on + strand, within B158DRAFT_2052 at 186.205 kb on + strand, within B158DRAFT_2052 at 186.205 kb on + strand, within B158DRAFT_2052 at 186.210 kb on + strand, within B158DRAFT_2052 at 186.213 kb on - strand, within B158DRAFT_2052 at 186.213 kb on - strand, within B158DRAFT_2052 at 186.213 kb on - strand, within B158DRAFT_2052 at 186.213 kb on - strand, within B158DRAFT_2052 at 186.213 kb on - strand, within B158DRAFT_2052 at 186.215 kb on + strand, within B158DRAFT_2052 at 186.215 kb on + strand, within B158DRAFT_2052 at 186.223 kb on - strand, within B158DRAFT_2052 at 186.223 kb on - strand, within B158DRAFT_2052 at 186.223 kb on - strand, within B158DRAFT_2052 at 186.225 kb on + strand, within B158DRAFT_2052 at 186.233 kb on - strand, within B158DRAFT_2052 at 186.236 kb on + strand, within B158DRAFT_2052 at 186.244 kb on - strand, within B158DRAFT_2052 at 186.298 kb on + strand, within B158DRAFT_2052 at 186.306 kb on - strand, within B158DRAFT_2052 at 186.377 kb on + strand, within B158DRAFT_2052 at 186.377 kb on + strand, within B158DRAFT_2052 at 186.459 kb on + strand, within B158DRAFT_2052 at 186.459 kb on + strand, within B158DRAFT_2052 at 186.459 kb on + strand, within B158DRAFT_2052 at 186.459 kb on + strand, within B158DRAFT_2052 at 186.459 kb on + strand, within B158DRAFT_2052 at 186.459 kb on + strand, within B158DRAFT_2052 at 186.459 kb on + strand, within B158DRAFT_2052 at 186.459 kb on + strand, within B158DRAFT_2052 at 186.467 kb on - strand, within B158DRAFT_2052 at 186.469 kb on + strand, within B158DRAFT_2052 at 186.477 kb on - strand, within B158DRAFT_2052 at 186.477 kb on - strand, within B158DRAFT_2052 at 186.477 kb on - strand, within B158DRAFT_2052 at 186.477 kb on - strand, within B158DRAFT_2052 at 186.477 kb on - strand, within B158DRAFT_2052 at 186.590 kb on + strand, within B158DRAFT_2052 at 186.688 kb on + strand, within B158DRAFT_2052 at 186.688 kb on + strand, within B158DRAFT_2052 at 186.745 kb on + strand, within B158DRAFT_2052 at 186.750 kb on + strand, within B158DRAFT_2052 at 186.751 kb on + strand, within B158DRAFT_2052 at 186.753 kb on - strand, within B158DRAFT_2052 at 186.766 kb on - strand, within B158DRAFT_2052
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 184,784 + B158DRAFT_2050 0.57 -0.1 184,784 + B158DRAFT_2050 0.57 +0.5 184,789 + B158DRAFT_2050 0.57 +0.6 184,982 + B158DRAFT_2050 0.67 -0.1 185,447 - -0.5 185,547 - +1.6 185,548 + +0.7 185,555 + +4.9 185,556 - -1.8 185,911 + -0.8 185,919 - +1.8 185,919 - -0.5 185,940 - +0.9 185,957 + -0.5 185,965 - -0.9 185,965 - -2.1 186,055 + B158DRAFT_2052 0.13 -2.2 186,055 + B158DRAFT_2052 0.13 -1.3 186,063 - B158DRAFT_2052 0.14 +0.0 186,076 + B158DRAFT_2052 0.15 +0.4 186,178 - B158DRAFT_2052 0.24 -1.4 186,179 - B158DRAFT_2052 0.24 -1.1 186,181 + B158DRAFT_2052 0.24 -0.3 186,181 + B158DRAFT_2052 0.24 +1.9 186,181 + B158DRAFT_2052 0.24 +2.6 186,181 + B158DRAFT_2052 0.24 -0.5 186,189 - B158DRAFT_2052 0.25 +0.6 186,189 - B158DRAFT_2052 0.25 -1.6 186,189 - B158DRAFT_2052 0.25 -0.7 186,189 - B158DRAFT_2052 0.25 -1.4 186,189 - B158DRAFT_2052 0.25 +1.0 186,205 + B158DRAFT_2052 0.27 -0.5 186,205 + B158DRAFT_2052 0.27 -3.5 186,205 + B158DRAFT_2052 0.27 +0.4 186,210 + B158DRAFT_2052 0.27 +0.1 186,213 - B158DRAFT_2052 0.27 -0.5 186,213 - B158DRAFT_2052 0.27 -0.9 186,213 - B158DRAFT_2052 0.27 +0.8 186,213 - B158DRAFT_2052 0.27 +3.2 186,213 - B158DRAFT_2052 0.27 -0.6 186,215 + B158DRAFT_2052 0.28 -2.0 186,215 + B158DRAFT_2052 0.28 -0.7 186,223 - B158DRAFT_2052 0.28 -1.4 186,223 - B158DRAFT_2052 0.28 -0.2 186,223 - B158DRAFT_2052 0.28 +0.7 186,225 + B158DRAFT_2052 0.28 +0.0 186,233 - B158DRAFT_2052 0.29 +0.2 186,236 + B158DRAFT_2052 0.29 -1.3 186,244 - B158DRAFT_2052 0.30 -0.9 186,298 + B158DRAFT_2052 0.35 -0.8 186,306 - B158DRAFT_2052 0.36 -0.6 186,377 + B158DRAFT_2052 0.42 -0.4 186,377 + B158DRAFT_2052 0.42 +0.2 186,459 + B158DRAFT_2052 0.50 -1.3 186,459 + B158DRAFT_2052 0.50 -1.2 186,459 + B158DRAFT_2052 0.50 +1.3 186,459 + B158DRAFT_2052 0.50 -1.4 186,459 + B158DRAFT_2052 0.50 +1.0 186,459 + B158DRAFT_2052 0.50 -0.7 186,459 + B158DRAFT_2052 0.50 -0.2 186,459 + B158DRAFT_2052 0.50 -0.3 186,467 - B158DRAFT_2052 0.51 +0.3 186,469 + B158DRAFT_2052 0.51 -2.5 186,477 - B158DRAFT_2052 0.52 -1.9 186,477 - B158DRAFT_2052 0.52 -0.8 186,477 - B158DRAFT_2052 0.52 -2.0 186,477 - B158DRAFT_2052 0.52 +0.0 186,477 - B158DRAFT_2052 0.52 +0.4 186,590 + B158DRAFT_2052 0.62 -1.4 186,688 + B158DRAFT_2052 0.71 -2.0 186,688 + B158DRAFT_2052 0.71 -0.2 186,745 + B158DRAFT_2052 0.76 -0.8 186,750 + B158DRAFT_2052 0.77 -1.1 186,751 + B158DRAFT_2052 0.77 +0.2 186,753 - B158DRAFT_2052 0.77 -1.7 186,766 - B158DRAFT_2052 0.78 +1.4
Or see this region's nucleotide sequence