Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_2023 and B158DRAFT_2024 are separated by 69 nucleotides B158DRAFT_2024 and B158DRAFT_2025 are separated by 76 nucleotides B158DRAFT_2025 and B158DRAFT_2026 are separated by 41 nucleotides
B158DRAFT_2023: B158DRAFT_2023 - Zn-dependent hydrolases, including glyoxylases, at 153,250 to 154,173
_2023
B158DRAFT_2024: B158DRAFT_2024 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 154,243 to 154,977
_2024
B158DRAFT_2025: B158DRAFT_2025 - Uncharacterized protein conserved in bacteria, at 155,054 to 155,488
_2025
B158DRAFT_2026: B158DRAFT_2026 - Uncharacterized protein conserved in bacteria, at 155,530 to 156,549
_2026
Position (kb) 155
156 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 154.168 kb on + strand at 154.177 kb on + strand at 154.185 kb on - strand at 154.273 kb on + strand at 154.315 kb on + strand at 154.322 kb on - strand, within B158DRAFT_2024 at 154.322 kb on - strand, within B158DRAFT_2024 at 154.322 kb on - strand, within B158DRAFT_2024 at 154.323 kb on + strand, within B158DRAFT_2024 at 154.545 kb on + strand, within B158DRAFT_2024 at 154.597 kb on - strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.599 kb on + strand, within B158DRAFT_2024 at 154.605 kb on + strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.607 kb on - strand, within B158DRAFT_2024 at 154.640 kb on - strand, within B158DRAFT_2024 at 154.649 kb on - strand, within B158DRAFT_2024 at 154.733 kb on - strand, within B158DRAFT_2024 at 154.748 kb on + strand, within B158DRAFT_2024 at 154.784 kb on + strand, within B158DRAFT_2024 at 154.794 kb on + strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.802 kb on - strand, within B158DRAFT_2024 at 154.803 kb on - strand, within B158DRAFT_2024 at 154.813 kb on + strand, within B158DRAFT_2024 at 154.813 kb on + strand, within B158DRAFT_2024 at 154.821 kb on - strand, within B158DRAFT_2024 at 154.828 kb on + strand, within B158DRAFT_2024 at 154.831 kb on - strand, within B158DRAFT_2024 at 154.841 kb on + strand, within B158DRAFT_2024 at 154.932 kb on + strand at 155.000 kb on - strand at 155.010 kb on + strand at 155.187 kb on - strand, within B158DRAFT_2025 at 155.256 kb on + strand, within B158DRAFT_2025 at 155.256 kb on + strand, within B158DRAFT_2025 at 155.256 kb on + strand, within B158DRAFT_2025 at 155.280 kb on + strand, within B158DRAFT_2025 at 155.310 kb on - strand, within B158DRAFT_2025 at 155.385 kb on + strand, within B158DRAFT_2025 at 155.385 kb on + strand, within B158DRAFT_2025 at 155.385 kb on + strand, within B158DRAFT_2025 at 155.393 kb on - strand, within B158DRAFT_2025 at 155.393 kb on - strand, within B158DRAFT_2025 at 155.397 kb on + strand, within B158DRAFT_2025 at 155.408 kb on + strand, within B158DRAFT_2025 at 155.415 kb on - strand, within B158DRAFT_2025 at 155.463 kb on + strand at 155.492 kb on - strand at 155.502 kb on - strand at 155.517 kb on - strand at 155.517 kb on - strand at 155.532 kb on + strand at 155.573 kb on + strand at 155.581 kb on - strand at 155.677 kb on + strand, within B158DRAFT_2026 at 155.687 kb on + strand, within B158DRAFT_2026 at 155.687 kb on + strand, within B158DRAFT_2026 at 155.687 kb on + strand, within B158DRAFT_2026 at 155.695 kb on - strand, within B158DRAFT_2026 at 155.695 kb on - strand, within B158DRAFT_2026 at 155.893 kb on + strand, within B158DRAFT_2026 at 155.894 kb on - strand, within B158DRAFT_2026 at 155.918 kb on - strand, within B158DRAFT_2026 at 156.013 kb on + strand, within B158DRAFT_2026 at 156.031 kb on - strand, within B158DRAFT_2026 at 156.115 kb on - strand, within B158DRAFT_2026 at 156.151 kb on - strand, within B158DRAFT_2026 at 156.169 kb on - strand, within B158DRAFT_2026 at 156.187 kb on + strand, within B158DRAFT_2026 at 156.192 kb on + strand, within B158DRAFT_2026 at 156.195 kb on - strand, within B158DRAFT_2026 at 156.203 kb on + strand, within B158DRAFT_2026 at 156.211 kb on - strand, within B158DRAFT_2026 at 156.213 kb on + strand, within B158DRAFT_2026 at 156.213 kb on + strand, within B158DRAFT_2026 at 156.213 kb on - strand, within B158DRAFT_2026 at 156.225 kb on - strand, within B158DRAFT_2026 at 156.240 kb on + strand, within B158DRAFT_2026 at 156.251 kb on + strand, within B158DRAFT_2026 at 156.252 kb on - strand, within B158DRAFT_2026 at 156.296 kb on + strand, within B158DRAFT_2026 at 156.408 kb on + strand, within B158DRAFT_2026 at 156.477 kb on + strand at 156.483 kb on - strand at 156.485 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 154,168 + +3.9 154,177 + +1.6 154,185 - +0.0 154,273 + +1.0 154,315 + -0.6 154,322 - B158DRAFT_2024 0.11 +0.2 154,322 - B158DRAFT_2024 0.11 -0.1 154,322 - B158DRAFT_2024 0.11 -0.2 154,323 + B158DRAFT_2024 0.11 -0.2 154,545 + B158DRAFT_2024 0.41 -2.5 154,597 - B158DRAFT_2024 0.48 +0.5 154,599 + B158DRAFT_2024 0.48 -0.8 154,599 + B158DRAFT_2024 0.48 +0.5 154,599 + B158DRAFT_2024 0.48 -1.0 154,599 + B158DRAFT_2024 0.48 -1.6 154,599 + B158DRAFT_2024 0.48 +0.7 154,599 + B158DRAFT_2024 0.48 +0.3 154,605 + B158DRAFT_2024 0.49 -1.6 154,607 - B158DRAFT_2024 0.50 -0.4 154,607 - B158DRAFT_2024 0.50 -0.4 154,607 - B158DRAFT_2024 0.50 -0.9 154,640 - B158DRAFT_2024 0.54 -0.4 154,649 - B158DRAFT_2024 0.55 -1.7 154,733 - B158DRAFT_2024 0.67 +2.6 154,748 + B158DRAFT_2024 0.69 +1.2 154,784 + B158DRAFT_2024 0.74 +4.2 154,794 + B158DRAFT_2024 0.75 +0.6 154,802 - B158DRAFT_2024 0.76 -1.0 154,802 - B158DRAFT_2024 0.76 -0.4 154,802 - B158DRAFT_2024 0.76 -0.8 154,803 - B158DRAFT_2024 0.76 -0.2 154,813 + B158DRAFT_2024 0.78 -0.2 154,813 + B158DRAFT_2024 0.78 -0.4 154,821 - B158DRAFT_2024 0.79 -1.0 154,828 + B158DRAFT_2024 0.80 -0.0 154,831 - B158DRAFT_2024 0.80 +0.4 154,841 + B158DRAFT_2024 0.81 +0.1 154,932 + -0.8 155,000 - -2.7 155,010 + +0.4 155,187 - B158DRAFT_2025 0.31 +0.3 155,256 + B158DRAFT_2025 0.46 +0.3 155,256 + B158DRAFT_2025 0.46 +0.0 155,256 + B158DRAFT_2025 0.46 -3.1 155,280 + B158DRAFT_2025 0.52 -1.5 155,310 - B158DRAFT_2025 0.59 -2.4 155,385 + B158DRAFT_2025 0.76 -0.0 155,385 + B158DRAFT_2025 0.76 +0.4 155,385 + B158DRAFT_2025 0.76 +0.9 155,393 - B158DRAFT_2025 0.78 -1.1 155,393 - B158DRAFT_2025 0.78 +1.6 155,397 + B158DRAFT_2025 0.79 -1.4 155,408 + B158DRAFT_2025 0.81 -1.8 155,415 - B158DRAFT_2025 0.83 -2.5 155,463 + +0.2 155,492 - -1.4 155,502 - -0.9 155,517 - +0.7 155,517 - -1.1 155,532 + -2.3 155,573 + -1.9 155,581 - -0.2 155,677 + B158DRAFT_2026 0.14 -3.1 155,687 + B158DRAFT_2026 0.15 -0.1 155,687 + B158DRAFT_2026 0.15 -0.2 155,687 + B158DRAFT_2026 0.15 -1.4 155,695 - B158DRAFT_2026 0.16 -1.9 155,695 - B158DRAFT_2026 0.16 -0.3 155,893 + B158DRAFT_2026 0.36 -1.9 155,894 - B158DRAFT_2026 0.36 +0.9 155,918 - B158DRAFT_2026 0.38 -0.8 156,013 + B158DRAFT_2026 0.47 -0.3 156,031 - B158DRAFT_2026 0.49 +1.0 156,115 - B158DRAFT_2026 0.57 +1.3 156,151 - B158DRAFT_2026 0.61 -0.5 156,169 - B158DRAFT_2026 0.63 -0.1 156,187 + B158DRAFT_2026 0.64 -1.9 156,192 + B158DRAFT_2026 0.65 -0.8 156,195 - B158DRAFT_2026 0.65 -0.8 156,203 + B158DRAFT_2026 0.66 +1.6 156,211 - B158DRAFT_2026 0.67 -0.6 156,213 + B158DRAFT_2026 0.67 -0.8 156,213 + B158DRAFT_2026 0.67 +1.1 156,213 - B158DRAFT_2026 0.67 -1.6 156,225 - B158DRAFT_2026 0.68 -1.0 156,240 + B158DRAFT_2026 0.70 -0.8 156,251 + B158DRAFT_2026 0.71 +0.4 156,252 - B158DRAFT_2026 0.71 -0.8 156,296 + B158DRAFT_2026 0.75 +0.6 156,408 + B158DRAFT_2026 0.86 -2.0 156,477 + -2.1 156,483 - -2.0 156,485 - +2.6
Or see this region's nucleotide sequence