Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1931 and B158DRAFT_1932 are separated by 3 nucleotides B158DRAFT_1932 and B158DRAFT_1933 are separated by 79 nucleotides B158DRAFT_1933 and B158DRAFT_1934 are separated by 209 nucleotides B158DRAFT_1934 and B158DRAFT_1935 overlap by 8 nucleotides
B158DRAFT_1931: B158DRAFT_1931 - Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase., at 66,187 to 67,422
_1931
B158DRAFT_1932: B158DRAFT_1932 - Response regulator consisting of a CheY-like receiver domain and a Fis-type HTH domain, at 67,426 to 68,001
_1932
B158DRAFT_1933: B158DRAFT_1933 - Outer membrane receptor for Fe3+-dicitrate, at 68,081 to 70,303
_1933
B158DRAFT_1934: B158DRAFT_1934 - Protein of unknown function (DUF2889)., at 70,513 to 71,088
_1934
B158DRAFT_1935: B158DRAFT_1935 - Uncharacterized protein conserved in bacteria, at 71,081 to 71,419
_1935
Position (kb) 68
69
70
71 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 67.228 kb on + strand, within B158DRAFT_1931 at 67.236 kb on - strand, within B158DRAFT_1931 at 67.236 kb on - strand, within B158DRAFT_1931 at 67.284 kb on + strand, within B158DRAFT_1931 at 67.285 kb on + strand, within B158DRAFT_1931 at 67.285 kb on + strand, within B158DRAFT_1931 at 67.285 kb on + strand, within B158DRAFT_1931 at 67.348 kb on + strand at 67.387 kb on + strand at 67.440 kb on + strand at 67.452 kb on + strand at 67.457 kb on + strand at 67.470 kb on - strand at 67.526 kb on + strand, within B158DRAFT_1932 at 67.526 kb on + strand, within B158DRAFT_1932 at 67.526 kb on + strand, within B158DRAFT_1932 at 67.534 kb on - strand, within B158DRAFT_1932 at 67.534 kb on - strand, within B158DRAFT_1932 at 67.536 kb on + strand, within B158DRAFT_1932 at 67.544 kb on + strand, within B158DRAFT_1932 at 67.544 kb on - strand, within B158DRAFT_1932 at 67.544 kb on - strand, within B158DRAFT_1932 at 67.544 kb on - strand, within B158DRAFT_1932 at 67.606 kb on + strand, within B158DRAFT_1932 at 67.614 kb on - strand, within B158DRAFT_1932 at 67.614 kb on - strand, within B158DRAFT_1932 at 67.614 kb on - strand, within B158DRAFT_1932 at 67.625 kb on + strand, within B158DRAFT_1932 at 67.625 kb on + strand, within B158DRAFT_1932 at 67.633 kb on - strand, within B158DRAFT_1932 at 67.635 kb on + strand, within B158DRAFT_1932 at 67.640 kb on + strand, within B158DRAFT_1932 at 67.643 kb on - strand, within B158DRAFT_1932 at 67.688 kb on + strand, within B158DRAFT_1932 at 67.696 kb on - strand, within B158DRAFT_1932 at 67.696 kb on - strand, within B158DRAFT_1932 at 67.696 kb on - strand, within B158DRAFT_1932 at 67.786 kb on + strand, within B158DRAFT_1932 at 67.826 kb on + strand, within B158DRAFT_1932 at 67.870 kb on + strand, within B158DRAFT_1932 at 67.914 kb on + strand, within B158DRAFT_1932 at 67.953 kb on + strand at 67.953 kb on - strand at 67.953 kb on - strand at 67.958 kb on + strand at 67.958 kb on + strand at 68.066 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.071 kb on + strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.079 kb on - strand at 68.132 kb on - strand at 68.132 kb on - strand at 68.155 kb on + strand at 68.182 kb on + strand at 68.209 kb on + strand at 68.212 kb on + strand at 68.217 kb on - strand at 68.219 kb on + strand at 68.219 kb on + strand at 68.219 kb on + strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.227 kb on - strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.228 kb on + strand at 68.235 kb on + strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.236 kb on - strand at 68.238 kb on + strand at 68.272 kb on + strand at 68.293 kb on - strand at 68.300 kb on + strand at 68.308 kb on - strand, within B158DRAFT_1933 at 68.310 kb on + strand, within B158DRAFT_1933 at 68.318 kb on - strand, within B158DRAFT_1933 at 68.323 kb on - strand, within B158DRAFT_1933 at 68.340 kb on + strand, within B158DRAFT_1933 at 68.348 kb on - strand, within B158DRAFT_1933 at 68.348 kb on - strand, within B158DRAFT_1933 at 68.373 kb on + strand, within B158DRAFT_1933 at 68.386 kb on - strand, within B158DRAFT_1933 at 68.386 kb on - strand, within B158DRAFT_1933 at 68.429 kb on + strand, within B158DRAFT_1933 at 68.471 kb on + strand, within B158DRAFT_1933 at 68.472 kb on + strand, within B158DRAFT_1933 at 68.472 kb on + strand, within B158DRAFT_1933 at 68.480 kb on - strand, within B158DRAFT_1933 at 68.480 kb on - strand, within B158DRAFT_1933 at 68.490 kb on + strand, within B158DRAFT_1933 at 68.542 kb on + strand, within B158DRAFT_1933 at 68.594 kb on - strand, within B158DRAFT_1933 at 68.594 kb on - strand, within B158DRAFT_1933 at 68.706 kb on + strand, within B158DRAFT_1933 at 68.853 kb on + strand, within B158DRAFT_1933 at 68.853 kb on + strand, within B158DRAFT_1933 at 68.864 kb on + strand, within B158DRAFT_1933 at 68.948 kb on - strand, within B158DRAFT_1933 at 68.980 kb on - strand, within B158DRAFT_1933 at 68.985 kb on + strand, within B158DRAFT_1933 at 68.985 kb on + strand, within B158DRAFT_1933 at 69.023 kb on + strand, within B158DRAFT_1933 at 69.034 kb on + strand, within B158DRAFT_1933 at 69.034 kb on + strand, within B158DRAFT_1933 at 69.074 kb on - strand, within B158DRAFT_1933 at 69.097 kb on - strand, within B158DRAFT_1933 at 69.107 kb on - strand, within B158DRAFT_1933 at 69.107 kb on - strand, within B158DRAFT_1933 at 69.168 kb on + strand, within B158DRAFT_1933 at 69.168 kb on + strand, within B158DRAFT_1933 at 69.168 kb on + strand, within B158DRAFT_1933 at 69.168 kb on + strand, within B158DRAFT_1933 at 69.176 kb on - strand, within B158DRAFT_1933 at 69.176 kb on - strand, within B158DRAFT_1933 at 69.176 kb on - strand, within B158DRAFT_1933 at 69.176 kb on - strand, within B158DRAFT_1933 at 69.331 kb on + strand, within B158DRAFT_1933 at 69.363 kb on - strand, within B158DRAFT_1933 at 69.379 kb on + strand, within B158DRAFT_1933 at 69.398 kb on + strand, within B158DRAFT_1933 at 69.398 kb on + strand, within B158DRAFT_1933 at 69.398 kb on + strand, within B158DRAFT_1933 at 69.424 kb on + strand, within B158DRAFT_1933 at 69.477 kb on + strand, within B158DRAFT_1933 at 69.512 kb on + strand, within B158DRAFT_1933 at 69.520 kb on - strand, within B158DRAFT_1933 at 69.533 kb on + strand, within B158DRAFT_1933 at 69.599 kb on + strand, within B158DRAFT_1933 at 69.599 kb on + strand, within B158DRAFT_1933 at 69.600 kb on - strand, within B158DRAFT_1933 at 69.627 kb on + strand, within B158DRAFT_1933 at 69.643 kb on + strand, within B158DRAFT_1933 at 69.643 kb on + strand, within B158DRAFT_1933 at 69.651 kb on - strand, within B158DRAFT_1933 at 69.662 kb on + strand, within B158DRAFT_1933 at 69.736 kb on - strand, within B158DRAFT_1933 at 69.799 kb on + strand, within B158DRAFT_1933 at 69.838 kb on + strand, within B158DRAFT_1933 at 69.867 kb on + strand, within B158DRAFT_1933 at 69.867 kb on + strand, within B158DRAFT_1933 at 69.867 kb on + strand, within B158DRAFT_1933 at 69.867 kb on + strand, within B158DRAFT_1933 at 69.867 kb on + strand, within B158DRAFT_1933 at 69.867 kb on + strand, within B158DRAFT_1933 at 69.875 kb on - strand, within B158DRAFT_1933 at 69.875 kb on - strand, within B158DRAFT_1933 at 69.998 kb on + strand, within B158DRAFT_1933 at 70.006 kb on - strand, within B158DRAFT_1933 at 70.009 kb on - strand, within B158DRAFT_1933 at 70.045 kb on - strand, within B158DRAFT_1933 at 70.050 kb on - strand, within B158DRAFT_1933 at 70.070 kb on + strand, within B158DRAFT_1933 at 70.072 kb on + strand, within B158DRAFT_1933 at 70.078 kb on - strand, within B158DRAFT_1933 at 70.078 kb on - strand, within B158DRAFT_1933 at 70.313 kb on + strand at 70.321 kb on - strand at 70.388 kb on - strand at 70.409 kb on + strand at 70.612 kb on + strand, within B158DRAFT_1934 at 70.623 kb on + strand, within B158DRAFT_1934 at 70.688 kb on + strand, within B158DRAFT_1934 at 70.696 kb on - strand, within B158DRAFT_1934 at 70.696 kb on - strand, within B158DRAFT_1934 at 70.750 kb on - strand, within B158DRAFT_1934 at 70.799 kb on - strand, within B158DRAFT_1934 at 70.800 kb on + strand, within B158DRAFT_1934 at 70.808 kb on - strand, within B158DRAFT_1934 at 70.808 kb on - strand, within B158DRAFT_1934 at 70.815 kb on - strand, within B158DRAFT_1934 at 70.840 kb on + strand, within B158DRAFT_1934 at 70.859 kb on + strand, within B158DRAFT_1934 at 70.859 kb on + strand, within B158DRAFT_1934 at 70.859 kb on + strand, within B158DRAFT_1934 at 70.859 kb on + strand, within B158DRAFT_1934 at 70.861 kb on + strand, within B158DRAFT_1934 at 70.861 kb on + strand, within B158DRAFT_1934 at 70.861 kb on + strand, within B158DRAFT_1934 at 70.861 kb on + strand, within B158DRAFT_1934 at 70.867 kb on - strand, within B158DRAFT_1934 at 70.869 kb on - strand, within B158DRAFT_1934 at 70.869 kb on - strand, within B158DRAFT_1934 at 70.869 kb on - strand, within B158DRAFT_1934 at 70.869 kb on - strand, within B158DRAFT_1934 at 70.869 kb on - strand, within B158DRAFT_1934 at 70.869 kb on - strand, within B158DRAFT_1934 at 70.871 kb on + strand, within B158DRAFT_1934 at 70.871 kb on + strand, within B158DRAFT_1934 at 70.879 kb on - strand, within B158DRAFT_1934 at 70.879 kb on - strand, within B158DRAFT_1934 at 70.879 kb on - strand, within B158DRAFT_1934 at 71.145 kb on - strand, within B158DRAFT_1935 at 71.235 kb on + strand, within B158DRAFT_1935
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 67,228 + B158DRAFT_1931 0.84 +3.8 67,236 - B158DRAFT_1931 0.85 +3.1 67,236 - B158DRAFT_1931 0.85 +3.2 67,284 + B158DRAFT_1931 0.89 +3.2 67,285 + B158DRAFT_1931 0.89 +2.8 67,285 + B158DRAFT_1931 0.89 +3.1 67,285 + B158DRAFT_1931 0.89 +3.5 67,348 + +4.8 67,387 + +3.2 67,440 + +3.0 67,452 + +3.8 67,457 + +4.5 67,470 - +3.2 67,526 + B158DRAFT_1932 0.17 +1.2 67,526 + B158DRAFT_1932 0.17 +3.2 67,526 + B158DRAFT_1932 0.17 +4.1 67,534 - B158DRAFT_1932 0.19 +3.2 67,534 - B158DRAFT_1932 0.19 +4.5 67,536 + B158DRAFT_1932 0.19 +3.2 67,544 + B158DRAFT_1932 0.20 +3.5 67,544 - B158DRAFT_1932 0.20 +3.5 67,544 - B158DRAFT_1932 0.20 +3.1 67,544 - B158DRAFT_1932 0.20 +4.1 67,606 + B158DRAFT_1932 0.31 -0.5 67,614 - B158DRAFT_1932 0.33 +3.1 67,614 - B158DRAFT_1932 0.33 +3.6 67,614 - B158DRAFT_1932 0.33 +3.9 67,625 + B158DRAFT_1932 0.35 +3.7 67,625 + B158DRAFT_1932 0.35 +2.4 67,633 - B158DRAFT_1932 0.36 +2.9 67,635 + B158DRAFT_1932 0.36 +4.8 67,640 + B158DRAFT_1932 0.37 +3.6 67,643 - B158DRAFT_1932 0.38 +2.4 67,688 + B158DRAFT_1932 0.45 +4.0 67,696 - B158DRAFT_1932 0.47 +3.3 67,696 - B158DRAFT_1932 0.47 +3.3 67,696 - B158DRAFT_1932 0.47 +3.5 67,786 + B158DRAFT_1932 0.62 +2.9 67,826 + B158DRAFT_1932 0.69 +3.2 67,870 + B158DRAFT_1932 0.77 +3.0 67,914 + B158DRAFT_1932 0.85 +3.5 67,953 + +4.1 67,953 - +3.3 67,953 - +3.6 67,958 + +3.4 67,958 + +2.6 68,066 + +1.2 68,071 + -0.2 68,071 + -0.2 68,071 + +0.3 68,071 + -1.3 68,071 + -1.5 68,071 + -1.3 68,071 + +0.2 68,071 + +2.0 68,079 - -0.5 68,079 - -0.2 68,079 - -1.0 68,079 - +0.6 68,079 - +0.0 68,079 - -1.3 68,079 - -1.2 68,079 - -1.0 68,079 - +0.1 68,132 - -1.7 68,132 - -1.3 68,155 + +0.2 68,182 + +1.3 68,209 + -0.2 68,212 + -1.3 68,217 - +0.1 68,219 + -1.1 68,219 + -1.0 68,219 + +0.3 68,227 - -0.6 68,227 - -0.6 68,227 - -0.9 68,227 - -0.1 68,227 - +0.2 68,227 - +6.0 68,227 - -1.8 68,227 - -2.3 68,227 - -0.5 68,227 - +0.1 68,228 + +0.2 68,228 + +0.1 68,228 + +0.6 68,228 + -0.5 68,228 + -0.1 68,228 + +0.3 68,228 + -0.4 68,235 + -0.5 68,236 - -0.8 68,236 - +1.8 68,236 - +0.9 68,236 - -1.3 68,236 - +0.4 68,236 - +1.5 68,238 + -1.0 68,272 + -1.3 68,293 - -1.6 68,300 + -0.9 68,308 - B158DRAFT_1933 0.10 -0.6 68,310 + B158DRAFT_1933 0.10 -1.2 68,318 - B158DRAFT_1933 0.11 -0.9 68,323 - B158DRAFT_1933 0.11 +1.3 68,340 + B158DRAFT_1933 0.12 +0.1 68,348 - B158DRAFT_1933 0.12 -1.9 68,348 - B158DRAFT_1933 0.12 -1.4 68,373 + B158DRAFT_1933 0.13 -0.3 68,386 - B158DRAFT_1933 0.14 +0.9 68,386 - B158DRAFT_1933 0.14 +0.7 68,429 + B158DRAFT_1933 0.16 -2.2 68,471 + B158DRAFT_1933 0.18 +1.8 68,472 + B158DRAFT_1933 0.18 +0.0 68,472 + B158DRAFT_1933 0.18 -0.5 68,480 - B158DRAFT_1933 0.18 +0.4 68,480 - B158DRAFT_1933 0.18 +0.7 68,490 + B158DRAFT_1933 0.18 -0.8 68,542 + B158DRAFT_1933 0.21 -1.0 68,594 - B158DRAFT_1933 0.23 -0.6 68,594 - B158DRAFT_1933 0.23 -1.7 68,706 + B158DRAFT_1933 0.28 +2.5 68,853 + B158DRAFT_1933 0.35 +0.4 68,853 + B158DRAFT_1933 0.35 -0.6 68,864 + B158DRAFT_1933 0.35 +1.2 68,948 - B158DRAFT_1933 0.39 -1.4 68,980 - B158DRAFT_1933 0.40 -0.4 68,985 + B158DRAFT_1933 0.41 -0.2 68,985 + B158DRAFT_1933 0.41 -2.1 69,023 + B158DRAFT_1933 0.42 -0.5 69,034 + B158DRAFT_1933 0.43 -2.6 69,034 + B158DRAFT_1933 0.43 -1.2 69,074 - B158DRAFT_1933 0.45 +3.9 69,097 - B158DRAFT_1933 0.46 -0.5 69,107 - B158DRAFT_1933 0.46 +3.2 69,107 - B158DRAFT_1933 0.46 -0.4 69,168 + B158DRAFT_1933 0.49 -1.2 69,168 + B158DRAFT_1933 0.49 -0.2 69,168 + B158DRAFT_1933 0.49 -0.7 69,168 + B158DRAFT_1933 0.49 +0.4 69,176 - B158DRAFT_1933 0.49 +0.9 69,176 - B158DRAFT_1933 0.49 -0.1 69,176 - B158DRAFT_1933 0.49 -0.8 69,176 - B158DRAFT_1933 0.49 +1.0 69,331 + B158DRAFT_1933 0.56 -0.3 69,363 - B158DRAFT_1933 0.58 +0.9 69,379 + B158DRAFT_1933 0.58 +0.6 69,398 + B158DRAFT_1933 0.59 -0.5 69,398 + B158DRAFT_1933 0.59 -0.7 69,398 + B158DRAFT_1933 0.59 +0.0 69,424 + B158DRAFT_1933 0.60 -0.8 69,477 + B158DRAFT_1933 0.63 +1.5 69,512 + B158DRAFT_1933 0.64 -0.9 69,520 - B158DRAFT_1933 0.65 +0.2 69,533 + B158DRAFT_1933 0.65 -0.1 69,599 + B158DRAFT_1933 0.68 -0.1 69,599 + B158DRAFT_1933 0.68 +1.2 69,600 - B158DRAFT_1933 0.68 -0.7 69,627 + B158DRAFT_1933 0.70 -0.2 69,643 + B158DRAFT_1933 0.70 -1.2 69,643 + B158DRAFT_1933 0.70 -1.4 69,651 - B158DRAFT_1933 0.71 -1.3 69,662 + B158DRAFT_1933 0.71 -0.6 69,736 - B158DRAFT_1933 0.74 +0.5 69,799 + B158DRAFT_1933 0.77 -1.4 69,838 + B158DRAFT_1933 0.79 -1.0 69,867 + B158DRAFT_1933 0.80 -0.3 69,867 + B158DRAFT_1933 0.80 -1.1 69,867 + B158DRAFT_1933 0.80 -2.4 69,867 + B158DRAFT_1933 0.80 -2.0 69,867 + B158DRAFT_1933 0.80 -1.7 69,867 + B158DRAFT_1933 0.80 -0.3 69,875 - B158DRAFT_1933 0.81 +1.4 69,875 - B158DRAFT_1933 0.81 -1.1 69,998 + B158DRAFT_1933 0.86 -2.0 70,006 - B158DRAFT_1933 0.87 -3.6 70,009 - B158DRAFT_1933 0.87 +0.5 70,045 - B158DRAFT_1933 0.88 -1.1 70,050 - B158DRAFT_1933 0.89 -0.1 70,070 + B158DRAFT_1933 0.89 -2.5 70,072 + B158DRAFT_1933 0.90 -0.6 70,078 - B158DRAFT_1933 0.90 -0.1 70,078 - B158DRAFT_1933 0.90 -0.9 70,313 + -0.4 70,321 - -2.0 70,388 - +0.1 70,409 + +0.8 70,612 + B158DRAFT_1934 0.17 -0.3 70,623 + B158DRAFT_1934 0.19 -0.7 70,688 + B158DRAFT_1934 0.30 -1.8 70,696 - B158DRAFT_1934 0.32 -1.8 70,696 - B158DRAFT_1934 0.32 -1.8 70,750 - B158DRAFT_1934 0.41 -0.2 70,799 - B158DRAFT_1934 0.50 -2.4 70,800 + B158DRAFT_1934 0.50 -1.5 70,808 - B158DRAFT_1934 0.51 -0.7 70,808 - B158DRAFT_1934 0.51 -0.1 70,815 - B158DRAFT_1934 0.52 -2.0 70,840 + B158DRAFT_1934 0.57 -1.4 70,859 + B158DRAFT_1934 0.60 -2.2 70,859 + B158DRAFT_1934 0.60 -1.2 70,859 + B158DRAFT_1934 0.60 +0.9 70,859 + B158DRAFT_1934 0.60 -2.3 70,861 + B158DRAFT_1934 0.60 -1.5 70,861 + B158DRAFT_1934 0.60 -1.4 70,861 + B158DRAFT_1934 0.60 +1.6 70,861 + B158DRAFT_1934 0.60 +0.1 70,867 - B158DRAFT_1934 0.61 +1.9 70,869 - B158DRAFT_1934 0.62 -0.4 70,869 - B158DRAFT_1934 0.62 -3.0 70,869 - B158DRAFT_1934 0.62 -1.9 70,869 - B158DRAFT_1934 0.62 -1.1 70,869 - B158DRAFT_1934 0.62 -2.0 70,869 - B158DRAFT_1934 0.62 +2.1 70,871 + B158DRAFT_1934 0.62 +0.6 70,871 + B158DRAFT_1934 0.62 +3.1 70,879 - B158DRAFT_1934 0.64 +2.1 70,879 - B158DRAFT_1934 0.64 +0.0 70,879 - B158DRAFT_1934 0.64 -0.4 71,145 - B158DRAFT_1935 0.19 -1.9 71,235 + B158DRAFT_1935 0.45 -0.3
Or see this region's nucleotide sequence