Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1921 and B158DRAFT_1922 are separated by 300 nucleotides B158DRAFT_1922 and B158DRAFT_1923 are separated by 117 nucleotides B158DRAFT_1923 and B158DRAFT_1924 are separated by 93 nucleotides
B158DRAFT_1921: B158DRAFT_1921 - glutaminyl-tRNA synthetase (EC 6.1.1.18), at 55,714 to 57,426
_1921
B158DRAFT_1922: B158DRAFT_1922 - Uncharacterized protein involved in outer membrane biogenesis, at 57,727 to 59,574
_1922
B158DRAFT_1923: B158DRAFT_1923 - hypothetical protein, at 59,692 to 60,450
_1923
B158DRAFT_1924: B158DRAFT_1924 - Methyltransferase domain., at 60,544 to 61,176
_1924
Position (kb) 57
58
59
60 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 57.396 kb on + strand at 57.396 kb on + strand at 57.396 kb on + strand at 57.444 kb on - strand at 57.457 kb on + strand at 57.505 kb on - strand at 57.505 kb on - strand at 57.506 kb on - strand at 57.506 kb on - strand at 57.507 kb on + strand at 57.515 kb on - strand at 57.515 kb on - strand at 57.515 kb on - strand at 57.515 kb on - strand at 57.515 kb on - strand at 57.517 kb on + strand at 57.517 kb on + strand at 57.521 kb on + strand at 57.525 kb on - strand at 57.525 kb on - strand at 57.525 kb on - strand at 57.530 kb on - strand at 57.531 kb on + strand at 57.539 kb on - strand at 57.539 kb on - strand at 57.553 kb on - strand at 57.608 kb on - strand at 57.646 kb on - strand at 57.683 kb on + strand at 57.757 kb on - strand at 57.757 kb on - strand at 57.757 kb on - strand at 57.828 kb on + strand at 57.828 kb on + strand at 57.836 kb on - strand at 57.841 kb on - strand at 57.892 kb on + strand at 57.893 kb on + strand at 57.900 kb on - strand at 57.900 kb on - strand at 57.955 kb on + strand, within B158DRAFT_1922 at 57.970 kb on - strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.972 kb on + strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 57.980 kb on - strand, within B158DRAFT_1922 at 58.018 kb on - strand, within B158DRAFT_1922 at 58.084 kb on - strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.162 kb on + strand, within B158DRAFT_1922 at 58.167 kb on + strand, within B158DRAFT_1922 at 58.167 kb on + strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.170 kb on - strand, within B158DRAFT_1922 at 58.175 kb on - strand, within B158DRAFT_1922 at 58.227 kb on + strand, within B158DRAFT_1922 at 58.235 kb on - strand, within B158DRAFT_1922 at 58.289 kb on - strand, within B158DRAFT_1922 at 58.317 kb on + strand, within B158DRAFT_1922 at 58.320 kb on - strand, within B158DRAFT_1922 at 58.335 kb on - strand, within B158DRAFT_1922 at 58.335 kb on - strand, within B158DRAFT_1922 at 58.354 kb on - strand, within B158DRAFT_1922 at 58.359 kb on - strand, within B158DRAFT_1922 at 58.486 kb on - strand, within B158DRAFT_1922 at 58.515 kb on - strand, within B158DRAFT_1922 at 58.542 kb on - strand, within B158DRAFT_1922 at 58.549 kb on + strand, within B158DRAFT_1922 at 58.550 kb on - strand, within B158DRAFT_1922 at 58.557 kb on - strand, within B158DRAFT_1922 at 58.607 kb on - strand, within B158DRAFT_1922 at 58.634 kb on + strand, within B158DRAFT_1922 at 58.650 kb on - strand, within B158DRAFT_1922 at 58.658 kb on - strand, within B158DRAFT_1922 at 58.667 kb on + strand, within B158DRAFT_1922 at 58.677 kb on + strand, within B158DRAFT_1922 at 58.682 kb on + strand, within B158DRAFT_1922 at 58.683 kb on + strand, within B158DRAFT_1922 at 58.691 kb on - strand, within B158DRAFT_1922 at 58.737 kb on + strand, within B158DRAFT_1922 at 58.807 kb on - strand, within B158DRAFT_1922 at 58.809 kb on + strand, within B158DRAFT_1922 at 58.817 kb on - strand, within B158DRAFT_1922 at 58.834 kb on - strand, within B158DRAFT_1922 at 58.850 kb on - strand, within B158DRAFT_1922 at 58.914 kb on - strand, within B158DRAFT_1922 at 58.914 kb on - strand, within B158DRAFT_1922 at 58.919 kb on - strand, within B158DRAFT_1922 at 58.929 kb on - strand, within B158DRAFT_1922 at 59.066 kb on + strand, within B158DRAFT_1922 at 59.066 kb on + strand, within B158DRAFT_1922 at 59.089 kb on + strand, within B158DRAFT_1922 at 59.134 kb on + strand, within B158DRAFT_1922 at 59.134 kb on + strand, within B158DRAFT_1922 at 59.134 kb on + strand, within B158DRAFT_1922 at 59.142 kb on - strand, within B158DRAFT_1922 at 59.142 kb on - strand, within B158DRAFT_1922 at 59.148 kb on - strand, within B158DRAFT_1922 at 59.150 kb on + strand, within B158DRAFT_1922 at 59.158 kb on - strand, within B158DRAFT_1922 at 59.158 kb on - strand, within B158DRAFT_1922 at 59.172 kb on - strand, within B158DRAFT_1922 at 59.193 kb on + strand, within B158DRAFT_1922 at 59.212 kb on + strand, within B158DRAFT_1922 at 59.224 kb on + strand, within B158DRAFT_1922 at 59.260 kb on + strand, within B158DRAFT_1922 at 59.280 kb on + strand, within B158DRAFT_1922 at 59.326 kb on - strand, within B158DRAFT_1922 at 59.331 kb on + strand, within B158DRAFT_1922 at 59.331 kb on + strand, within B158DRAFT_1922 at 59.396 kb on + strand at 59.404 kb on - strand at 59.417 kb on + strand at 59.425 kb on - strand at 59.480 kb on - strand at 59.511 kb on + strand at 59.511 kb on + strand at 59.519 kb on - strand at 59.573 kb on - strand at 59.630 kb on + strand at 59.638 kb on - strand at 59.638 kb on - strand at 59.783 kb on + strand, within B158DRAFT_1923 at 59.783 kb on + strand, within B158DRAFT_1923 at 59.783 kb on + strand, within B158DRAFT_1923 at 59.804 kb on + strand, within B158DRAFT_1923 at 60.002 kb on - strand, within B158DRAFT_1923 at 60.049 kb on - strand, within B158DRAFT_1923 at 60.149 kb on + strand, within B158DRAFT_1923 at 60.215 kb on - strand, within B158DRAFT_1923 at 60.224 kb on + strand, within B158DRAFT_1923 at 60.377 kb on + strand at 60.381 kb on + strand at 60.466 kb on + strand at 60.466 kb on + strand at 60.466 kb on + strand at 60.474 kb on - strand at 60.474 kb on - strand at 60.474 kb on - strand at 60.474 kb on - strand at 60.542 kb on + strand at 60.542 kb on + strand at 60.550 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 57,396 + -0.8 57,396 + +1.0 57,396 + -0.1 57,444 - -1.3 57,457 + +0.1 57,505 - -0.2 57,505 - -0.4 57,506 - +0.5 57,506 - -0.8 57,507 + -2.6 57,515 - -0.3 57,515 - -0.8 57,515 - -1.5 57,515 - -2.3 57,515 - +0.4 57,517 + -1.9 57,517 + -0.1 57,521 + +0.1 57,525 - +0.0 57,525 - +0.2 57,525 - -1.2 57,530 - -1.1 57,531 + -2.2 57,539 - -1.9 57,539 - -0.5 57,553 - -0.9 57,608 - -0.1 57,646 - +4.2 57,683 + -0.9 57,757 - +0.8 57,757 - -1.5 57,757 - -1.9 57,828 + -2.0 57,828 + +0.9 57,836 - +0.0 57,841 - -0.3 57,892 + +0.7 57,893 + -0.1 57,900 - -0.2 57,900 - +0.2 57,955 + B158DRAFT_1922 0.12 -1.0 57,970 - B158DRAFT_1922 0.13 +0.0 57,972 + B158DRAFT_1922 0.13 -1.9 57,972 + B158DRAFT_1922 0.13 -0.7 57,972 + B158DRAFT_1922 0.13 -0.2 57,972 + B158DRAFT_1922 0.13 -1.1 57,972 + B158DRAFT_1922 0.13 -1.4 57,972 + B158DRAFT_1922 0.13 -0.7 57,972 + B158DRAFT_1922 0.13 -0.9 57,972 + B158DRAFT_1922 0.13 +0.2 57,972 + B158DRAFT_1922 0.13 -1.8 57,972 + B158DRAFT_1922 0.13 -1.1 57,972 + B158DRAFT_1922 0.13 -1.8 57,980 - B158DRAFT_1922 0.14 -0.2 57,980 - B158DRAFT_1922 0.14 -0.7 57,980 - B158DRAFT_1922 0.14 +0.2 57,980 - B158DRAFT_1922 0.14 +0.2 57,980 - B158DRAFT_1922 0.14 +1.6 57,980 - B158DRAFT_1922 0.14 +0.3 57,980 - B158DRAFT_1922 0.14 -0.7 57,980 - B158DRAFT_1922 0.14 -1.7 57,980 - B158DRAFT_1922 0.14 -2.3 58,018 - B158DRAFT_1922 0.16 +0.4 58,084 - B158DRAFT_1922 0.19 +0.4 58,162 + B158DRAFT_1922 0.24 -3.3 58,162 + B158DRAFT_1922 0.24 -0.5 58,162 + B158DRAFT_1922 0.24 -1.5 58,162 + B158DRAFT_1922 0.24 -2.4 58,162 + B158DRAFT_1922 0.24 +0.5 58,162 + B158DRAFT_1922 0.24 -2.0 58,167 + B158DRAFT_1922 0.24 -1.1 58,167 + B158DRAFT_1922 0.24 -0.4 58,170 - B158DRAFT_1922 0.24 -0.8 58,170 - B158DRAFT_1922 0.24 -1.8 58,170 - B158DRAFT_1922 0.24 -1.4 58,170 - B158DRAFT_1922 0.24 -0.6 58,170 - B158DRAFT_1922 0.24 +0.4 58,170 - B158DRAFT_1922 0.24 -1.8 58,175 - B158DRAFT_1922 0.24 -0.1 58,227 + B158DRAFT_1922 0.27 -2.3 58,235 - B158DRAFT_1922 0.27 -0.3 58,289 - B158DRAFT_1922 0.30 -0.1 58,317 + B158DRAFT_1922 0.32 -2.4 58,320 - B158DRAFT_1922 0.32 +1.4 58,335 - B158DRAFT_1922 0.33 -1.6 58,335 - B158DRAFT_1922 0.33 -1.5 58,354 - B158DRAFT_1922 0.34 -1.7 58,359 - B158DRAFT_1922 0.34 +0.7 58,486 - B158DRAFT_1922 0.41 -1.1 58,515 - B158DRAFT_1922 0.43 +1.6 58,542 - B158DRAFT_1922 0.44 +0.1 58,549 + B158DRAFT_1922 0.44 -1.9 58,550 - B158DRAFT_1922 0.45 -1.2 58,557 - B158DRAFT_1922 0.45 -1.1 58,607 - B158DRAFT_1922 0.48 +1.5 58,634 + B158DRAFT_1922 0.49 -0.5 58,650 - B158DRAFT_1922 0.50 -1.5 58,658 - B158DRAFT_1922 0.50 +0.5 58,667 + B158DRAFT_1922 0.51 +4.0 58,677 + B158DRAFT_1922 0.51 +0.3 58,682 + B158DRAFT_1922 0.52 -0.1 58,683 + B158DRAFT_1922 0.52 +0.7 58,691 - B158DRAFT_1922 0.52 -1.1 58,737 + B158DRAFT_1922 0.55 -1.3 58,807 - B158DRAFT_1922 0.58 -0.5 58,809 + B158DRAFT_1922 0.59 -0.1 58,817 - B158DRAFT_1922 0.59 -0.1 58,834 - B158DRAFT_1922 0.60 +2.4 58,850 - B158DRAFT_1922 0.61 +0.5 58,914 - B158DRAFT_1922 0.64 +0.4 58,914 - B158DRAFT_1922 0.64 -1.6 58,919 - B158DRAFT_1922 0.65 +0.1 58,929 - B158DRAFT_1922 0.65 +1.2 59,066 + B158DRAFT_1922 0.72 -0.2 59,066 + B158DRAFT_1922 0.72 +2.1 59,089 + B158DRAFT_1922 0.74 -3.1 59,134 + B158DRAFT_1922 0.76 +0.6 59,134 + B158DRAFT_1922 0.76 -1.1 59,134 + B158DRAFT_1922 0.76 -0.8 59,142 - B158DRAFT_1922 0.77 +0.6 59,142 - B158DRAFT_1922 0.77 -2.6 59,148 - B158DRAFT_1922 0.77 -2.0 59,150 + B158DRAFT_1922 0.77 +1.0 59,158 - B158DRAFT_1922 0.77 -1.8 59,158 - B158DRAFT_1922 0.77 +0.6 59,172 - B158DRAFT_1922 0.78 -0.3 59,193 + B158DRAFT_1922 0.79 +0.1 59,212 + B158DRAFT_1922 0.80 -1.8 59,224 + B158DRAFT_1922 0.81 -1.4 59,260 + B158DRAFT_1922 0.83 -1.0 59,280 + B158DRAFT_1922 0.84 -0.0 59,326 - B158DRAFT_1922 0.87 -1.3 59,331 + B158DRAFT_1922 0.87 -2.5 59,331 + B158DRAFT_1922 0.87 -0.1 59,396 + -1.5 59,404 - -1.7 59,417 + -0.8 59,425 - -2.5 59,480 - -2.5 59,511 + +0.2 59,511 + -1.9 59,519 - -1.0 59,573 - +0.9 59,630 + -2.1 59,638 - -0.1 59,638 - -2.3 59,783 + B158DRAFT_1923 0.12 +0.3 59,783 + B158DRAFT_1923 0.12 +1.5 59,783 + B158DRAFT_1923 0.12 -0.3 59,804 + B158DRAFT_1923 0.15 -0.4 60,002 - B158DRAFT_1923 0.41 +1.0 60,049 - B158DRAFT_1923 0.47 +4.2 60,149 + B158DRAFT_1923 0.60 +4.2 60,215 - B158DRAFT_1923 0.69 +3.2 60,224 + B158DRAFT_1923 0.70 +0.6 60,377 + +0.3 60,381 + +0.3 60,466 + -0.9 60,466 + -2.2 60,466 + -0.0 60,474 - -0.1 60,474 - -0.9 60,474 - +3.7 60,474 - +0.2 60,542 + +0.7 60,542 + -0.7 60,550 - -0.1
Or see this region's nucleotide sequence