Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1477 and B158DRAFT_1478 are separated by 419 nucleotides B158DRAFT_1478 and B158DRAFT_1479 are separated by 122 nucleotides
B158DRAFT_1477: B158DRAFT_1477 - pyruvate dehydrogenase E1 component, alpha subunit, at 1,529,394 to 1,530,485
_1477
B158DRAFT_1478: B158DRAFT_1478 - hypothetical protein, at 1,530,905 to 1,531,288
_1478
B158DRAFT_1479: B158DRAFT_1479 - aminopeptidase N, Escherichia coli type, at 1,531,411 to 1,534,077
_1479
Position (kb)
1530
1531
1532 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1530.172 kb on + strand, within B158DRAFT_1477 at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.503 kb on - strand at 1530.534 kb on - strand at 1530.704 kb on - strand at 1530.704 kb on - strand at 1530.777 kb on + strand at 1530.785 kb on - strand at 1530.958 kb on + strand, within B158DRAFT_1478 at 1530.966 kb on - strand, within B158DRAFT_1478 at 1530.968 kb on + strand, within B158DRAFT_1478 at 1530.968 kb on + strand, within B158DRAFT_1478 at 1530.982 kb on + strand, within B158DRAFT_1478 at 1530.990 kb on - strand, within B158DRAFT_1478 at 1531.004 kb on + strand, within B158DRAFT_1478 at 1531.004 kb on + strand, within B158DRAFT_1478 at 1531.004 kb on + strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.012 kb on - strand, within B158DRAFT_1478 at 1531.045 kb on + strand, within B158DRAFT_1478 at 1531.087 kb on + strand, within B158DRAFT_1478 at 1531.108 kb on + strand, within B158DRAFT_1478 at 1531.148 kb on + strand, within B158DRAFT_1478 at 1531.159 kb on + strand, within B158DRAFT_1478 at 1531.159 kb on + strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.167 kb on - strand, within B158DRAFT_1478 at 1531.190 kb on - strand, within B158DRAFT_1478 at 1531.190 kb on - strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.194 kb on + strand, within B158DRAFT_1478 at 1531.198 kb on + strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.202 kb on - strand, within B158DRAFT_1478 at 1531.203 kb on + strand, within B158DRAFT_1478 at 1531.217 kb on + strand, within B158DRAFT_1478 at 1531.217 kb on + strand, within B158DRAFT_1478 at 1531.271 kb on + strand at 1531.278 kb on + strand at 1531.286 kb on - strand at 1531.290 kb on + strand at 1531.441 kb on - strand at 1531.450 kb on + strand at 1531.458 kb on - strand at 1531.458 kb on - strand at 1531.553 kb on - strand at 1531.563 kb on + strand at 1531.563 kb on + strand at 1531.571 kb on - strand at 1531.579 kb on - strand at 1531.602 kb on - strand at 1531.610 kb on + strand at 1531.618 kb on - strand at 1531.624 kb on + strand at 1531.626 kb on + strand at 1531.632 kb on - strand at 1531.632 kb on - strand at 1531.655 kb on - strand at 1531.655 kb on - strand at 1531.663 kb on + strand at 1531.663 kb on + strand at 1531.666 kb on - strand at 1531.671 kb on - strand at 1531.671 kb on - strand at 1531.756 kb on + strand, within B158DRAFT_1479 at 1531.774 kb on + strand, within B158DRAFT_1479 at 1531.774 kb on + strand, within B158DRAFT_1479 at 1531.774 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.775 kb on + strand, within B158DRAFT_1479 at 1531.782 kb on - strand, within B158DRAFT_1479 at 1531.782 kb on - strand, within B158DRAFT_1479 at 1531.782 kb on - strand, within B158DRAFT_1479 at 1531.782 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.783 kb on - strand, within B158DRAFT_1479 at 1531.797 kb on + strand, within B158DRAFT_1479 at 1531.797 kb on + strand, within B158DRAFT_1479 at 1531.809 kb on + strand, within B158DRAFT_1479 at 1531.809 kb on + strand, within B158DRAFT_1479 at 1531.809 kb on + strand, within B158DRAFT_1479 at 1531.809 kb on + strand, within B158DRAFT_1479 at 1531.809 kb on + strand, within B158DRAFT_1479 at 1531.809 kb on + strand, within B158DRAFT_1479 at 1531.816 kb on + strand, within B158DRAFT_1479 at 1531.816 kb on + strand, within B158DRAFT_1479 at 1531.817 kb on - strand, within B158DRAFT_1479 at 1531.824 kb on - strand, within B158DRAFT_1479 at 1531.852 kb on - strand, within B158DRAFT_1479 at 1531.869 kb on - strand, within B158DRAFT_1479 at 1531.869 kb on - strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.871 kb on + strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.879 kb on - strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.920 kb on + strand, within B158DRAFT_1479 at 1531.928 kb on - strand, within B158DRAFT_1479 at 1531.928 kb on - strand, within B158DRAFT_1479 at 1531.928 kb on - strand, within B158DRAFT_1479 at 1531.928 kb on - strand, within B158DRAFT_1479 at 1531.947 kb on - strand, within B158DRAFT_1479 at 1531.947 kb on - strand, within B158DRAFT_1479 at 1532.027 kb on - strand, within B158DRAFT_1479 at 1532.108 kb on + strand, within B158DRAFT_1479 at 1532.108 kb on + strand, within B158DRAFT_1479 at 1532.116 kb on - strand, within B158DRAFT_1479 at 1532.116 kb on - strand, within B158DRAFT_1479 at 1532.139 kb on - strand, within B158DRAFT_1479 at 1532.286 kb on - strand, within B158DRAFT_1479 at 1532.286 kb on - strand, within B158DRAFT_1479
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 1,530,172 + B158DRAFT_1477 0.71 +1.7 1,530,503 - -0.2 1,530,503 - -0.5 1,530,503 - -0.4 1,530,503 - -1.4 1,530,503 - -2.0 1,530,503 - -3.5 1,530,503 - -0.4 1,530,503 - +0.1 1,530,503 - -0.7 1,530,503 - -0.6 1,530,534 - +0.4 1,530,704 - +1.5 1,530,704 - +2.9 1,530,777 + -1.1 1,530,785 - -0.2 1,530,958 + B158DRAFT_1478 0.14 +1.1 1,530,966 - B158DRAFT_1478 0.16 -1.5 1,530,968 + B158DRAFT_1478 0.16 -1.5 1,530,968 + B158DRAFT_1478 0.16 -0.8 1,530,982 + B158DRAFT_1478 0.20 +0.1 1,530,990 - B158DRAFT_1478 0.22 -1.4 1,531,004 + B158DRAFT_1478 0.26 +0.1 1,531,004 + B158DRAFT_1478 0.26 +2.0 1,531,004 + B158DRAFT_1478 0.26 -1.0 1,531,012 - B158DRAFT_1478 0.28 -1.0 1,531,012 - B158DRAFT_1478 0.28 -1.4 1,531,012 - B158DRAFT_1478 0.28 -1.7 1,531,012 - B158DRAFT_1478 0.28 +2.8 1,531,012 - B158DRAFT_1478 0.28 -0.1 1,531,012 - B158DRAFT_1478 0.28 -0.8 1,531,012 - B158DRAFT_1478 0.28 +0.9 1,531,012 - B158DRAFT_1478 0.28 -0.9 1,531,012 - B158DRAFT_1478 0.28 -2.3 1,531,012 - B158DRAFT_1478 0.28 +0.6 1,531,012 - B158DRAFT_1478 0.28 -0.6 1,531,045 + B158DRAFT_1478 0.36 -2.0 1,531,087 + B158DRAFT_1478 0.47 -2.0 1,531,108 + B158DRAFT_1478 0.53 +2.8 1,531,148 + B158DRAFT_1478 0.63 -0.4 1,531,159 + B158DRAFT_1478 0.66 -0.7 1,531,159 + B158DRAFT_1478 0.66 +0.2 1,531,167 - B158DRAFT_1478 0.68 +3.9 1,531,167 - B158DRAFT_1478 0.68 +1.2 1,531,167 - B158DRAFT_1478 0.68 +0.4 1,531,167 - B158DRAFT_1478 0.68 -1.3 1,531,190 - B158DRAFT_1478 0.74 -0.2 1,531,190 - B158DRAFT_1478 0.74 -0.6 1,531,194 + B158DRAFT_1478 0.75 +0.7 1,531,194 + B158DRAFT_1478 0.75 -1.4 1,531,194 + B158DRAFT_1478 0.75 +1.7 1,531,194 + B158DRAFT_1478 0.75 -1.1 1,531,198 + B158DRAFT_1478 0.76 +0.2 1,531,202 - B158DRAFT_1478 0.77 -2.2 1,531,202 - B158DRAFT_1478 0.77 +0.1 1,531,202 - B158DRAFT_1478 0.77 +0.1 1,531,202 - B158DRAFT_1478 0.77 -1.7 1,531,203 + B158DRAFT_1478 0.78 -1.3 1,531,217 + B158DRAFT_1478 0.81 +0.0 1,531,217 + B158DRAFT_1478 0.81 -1.6 1,531,271 + -1.2 1,531,278 + +0.9 1,531,286 - -1.2 1,531,290 + +0.7 1,531,441 - +0.1 1,531,450 + -1.8 1,531,458 - -1.7 1,531,458 - -1.0 1,531,553 - -2.0 1,531,563 + +1.4 1,531,563 + +0.2 1,531,571 - +0.2 1,531,579 - -2.6 1,531,602 - +3.3 1,531,610 + +1.1 1,531,618 - +0.3 1,531,624 + +0.3 1,531,626 + -0.0 1,531,632 - -1.6 1,531,632 - -1.3 1,531,655 - +1.1 1,531,655 - -0.4 1,531,663 + -2.2 1,531,663 + +0.5 1,531,666 - -1.0 1,531,671 - +1.2 1,531,671 - -0.4 1,531,756 + B158DRAFT_1479 0.13 +0.6 1,531,774 + B158DRAFT_1479 0.14 -0.7 1,531,774 + B158DRAFT_1479 0.14 -0.6 1,531,774 + B158DRAFT_1479 0.14 -2.1 1,531,775 + B158DRAFT_1479 0.14 -0.3 1,531,775 + B158DRAFT_1479 0.14 +0.4 1,531,775 + B158DRAFT_1479 0.14 -0.5 1,531,775 + B158DRAFT_1479 0.14 +0.7 1,531,775 + B158DRAFT_1479 0.14 -1.1 1,531,775 + B158DRAFT_1479 0.14 -0.4 1,531,775 + B158DRAFT_1479 0.14 -0.1 1,531,775 + B158DRAFT_1479 0.14 -1.7 1,531,775 + B158DRAFT_1479 0.14 +1.6 1,531,775 + B158DRAFT_1479 0.14 +0.9 1,531,775 + B158DRAFT_1479 0.14 +1.6 1,531,775 + B158DRAFT_1479 0.14 -0.1 1,531,775 + B158DRAFT_1479 0.14 +0.6 1,531,782 - B158DRAFT_1479 0.14 +1.1 1,531,782 - B158DRAFT_1479 0.14 -1.8 1,531,782 - B158DRAFT_1479 0.14 +0.6 1,531,782 - B158DRAFT_1479 0.14 +1.1 1,531,783 - B158DRAFT_1479 0.14 +0.1 1,531,783 - B158DRAFT_1479 0.14 -0.8 1,531,783 - B158DRAFT_1479 0.14 +3.1 1,531,783 - B158DRAFT_1479 0.14 -0.9 1,531,783 - B158DRAFT_1479 0.14 -1.3 1,531,783 - B158DRAFT_1479 0.14 -0.6 1,531,783 - B158DRAFT_1479 0.14 -0.6 1,531,783 - B158DRAFT_1479 0.14 +1.0 1,531,783 - B158DRAFT_1479 0.14 +0.0 1,531,783 - B158DRAFT_1479 0.14 +0.4 1,531,783 - B158DRAFT_1479 0.14 +0.6 1,531,783 - B158DRAFT_1479 0.14 -0.8 1,531,783 - B158DRAFT_1479 0.14 -0.1 1,531,783 - B158DRAFT_1479 0.14 +0.3 1,531,783 - B158DRAFT_1479 0.14 +0.0 1,531,783 - B158DRAFT_1479 0.14 -0.0 1,531,783 - B158DRAFT_1479 0.14 +0.1 1,531,783 - B158DRAFT_1479 0.14 +0.4 1,531,783 - B158DRAFT_1479 0.14 -0.6 1,531,783 - B158DRAFT_1479 0.14 -1.4 1,531,797 + B158DRAFT_1479 0.14 +0.7 1,531,797 + B158DRAFT_1479 0.14 -0.3 1,531,809 + B158DRAFT_1479 0.15 -0.1 1,531,809 + B158DRAFT_1479 0.15 +3.0 1,531,809 + B158DRAFT_1479 0.15 +1.3 1,531,809 + B158DRAFT_1479 0.15 -1.0 1,531,809 + B158DRAFT_1479 0.15 -0.3 1,531,809 + B158DRAFT_1479 0.15 -0.4 1,531,816 + B158DRAFT_1479 0.15 +2.0 1,531,816 + B158DRAFT_1479 0.15 +0.5 1,531,817 - B158DRAFT_1479 0.15 -0.7 1,531,824 - B158DRAFT_1479 0.15 -0.2 1,531,852 - B158DRAFT_1479 0.17 -2.2 1,531,869 - B158DRAFT_1479 0.17 -0.4 1,531,869 - B158DRAFT_1479 0.17 -0.4 1,531,871 + B158DRAFT_1479 0.17 +3.3 1,531,871 + B158DRAFT_1479 0.17 -1.2 1,531,871 + B158DRAFT_1479 0.17 -2.2 1,531,871 + B158DRAFT_1479 0.17 -0.3 1,531,871 + B158DRAFT_1479 0.17 -0.6 1,531,871 + B158DRAFT_1479 0.17 -0.2 1,531,871 + B158DRAFT_1479 0.17 +0.7 1,531,871 + B158DRAFT_1479 0.17 -1.0 1,531,871 + B158DRAFT_1479 0.17 -0.8 1,531,871 + B158DRAFT_1479 0.17 -1.0 1,531,879 - B158DRAFT_1479 0.18 +0.7 1,531,879 - B158DRAFT_1479 0.18 -0.2 1,531,879 - B158DRAFT_1479 0.18 +0.2 1,531,879 - B158DRAFT_1479 0.18 -1.7 1,531,879 - B158DRAFT_1479 0.18 -0.4 1,531,879 - B158DRAFT_1479 0.18 -1.9 1,531,879 - B158DRAFT_1479 0.18 -2.1 1,531,879 - B158DRAFT_1479 0.18 -0.3 1,531,879 - B158DRAFT_1479 0.18 -0.4 1,531,879 - B158DRAFT_1479 0.18 +1.7 1,531,879 - B158DRAFT_1479 0.18 -1.0 1,531,879 - B158DRAFT_1479 0.18 +0.6 1,531,879 - B158DRAFT_1479 0.18 +1.8 1,531,879 - B158DRAFT_1479 0.18 -0.4 1,531,879 - B158DRAFT_1479 0.18 +0.7 1,531,879 - B158DRAFT_1479 0.18 -2.0 1,531,879 - B158DRAFT_1479 0.18 -0.1 1,531,879 - B158DRAFT_1479 0.18 -0.2 1,531,879 - B158DRAFT_1479 0.18 -0.8 1,531,879 - B158DRAFT_1479 0.18 +0.7 1,531,920 + B158DRAFT_1479 0.19 +0.0 1,531,920 + B158DRAFT_1479 0.19 -1.0 1,531,920 + B158DRAFT_1479 0.19 +1.1 1,531,920 + B158DRAFT_1479 0.19 -0.1 1,531,920 + B158DRAFT_1479 0.19 +0.6 1,531,920 + B158DRAFT_1479 0.19 -0.4 1,531,920 + B158DRAFT_1479 0.19 -0.6 1,531,920 + B158DRAFT_1479 0.19 -1.0 1,531,920 + B158DRAFT_1479 0.19 -1.0 1,531,920 + B158DRAFT_1479 0.19 -0.5 1,531,928 - B158DRAFT_1479 0.19 -0.0 1,531,928 - B158DRAFT_1479 0.19 +1.8 1,531,928 - B158DRAFT_1479 0.19 +1.0 1,531,928 - B158DRAFT_1479 0.19 -0.1 1,531,947 - B158DRAFT_1479 0.20 -1.1 1,531,947 - B158DRAFT_1479 0.20 -0.2 1,532,027 - B158DRAFT_1479 0.23 +0.2 1,532,108 + B158DRAFT_1479 0.26 -0.4 1,532,108 + B158DRAFT_1479 0.26 +1.7 1,532,116 - B158DRAFT_1479 0.26 +0.1 1,532,116 - B158DRAFT_1479 0.26 -1.2 1,532,139 - B158DRAFT_1479 0.27 -1.5 1,532,286 - B158DRAFT_1479 0.33 +0.1 1,532,286 - B158DRAFT_1479 0.33 -0.1
Or see this region's nucleotide sequence