Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0742 and B158DRAFT_0743 are separated by 30 nucleotides B158DRAFT_0743 and B158DRAFT_0744 are separated by 24 nucleotides B158DRAFT_0744 and B158DRAFT_0745 are separated by 18 nucleotides B158DRAFT_0745 and B158DRAFT_0746 are separated by 171 nucleotides
B158DRAFT_0742: B158DRAFT_0742 - 2-octaprenylphenol hydroxylase (EC 1.14.13.-), at 745,235 to 746,881
_0742
B158DRAFT_0743: B158DRAFT_0743 - Uncharacterized protein conserved in bacteria, at 746,912 to 747,550
_0743
B158DRAFT_0744: B158DRAFT_0744 - ubiquinone/menaquinone biosynthesis methyltransferases, at 747,575 to 748,342
_0744
B158DRAFT_0745: B158DRAFT_0745 - Uncharacterized protein conserved in bacteria, at 748,361 to 748,765
_0745
B158DRAFT_0746: B158DRAFT_0746 - Subtilisin-like serine proteases, at 748,937 to 750,787
_0746
Position (kb)
747
748
749 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 746.978 kb on - strand, within B158DRAFT_0743 at 747.380 kb on - strand, within B158DRAFT_0743 at 747.476 kb on - strand, within B158DRAFT_0743 at 747.476 kb on - strand, within B158DRAFT_0743 at 747.538 kb on - strand at 747.566 kb on - strand at 748.352 kb on - strand at 748.352 kb on - strand at 748.352 kb on - strand at 748.465 kb on - strand, within B158DRAFT_0745 at 748.478 kb on - strand, within B158DRAFT_0745 at 748.499 kb on + strand, within B158DRAFT_0745 at 748.502 kb on - strand, within B158DRAFT_0745 at 748.519 kb on - strand, within B158DRAFT_0745 at 748.535 kb on - strand, within B158DRAFT_0745 at 748.602 kb on + strand, within B158DRAFT_0745 at 748.782 kb on + strand at 748.782 kb on + strand at 748.782 kb on + strand at 748.782 kb on + strand at 748.790 kb on - strand at 748.830 kb on + strand at 748.830 kb on - strand at 748.874 kb on + strand at 748.957 kb on + strand at 749.016 kb on + strand at 749.016 kb on + strand at 749.016 kb on + strand at 749.024 kb on - strand at 749.024 kb on - strand at 749.086 kb on + strand at 749.094 kb on + strand at 749.094 kb on - strand at 749.135 kb on + strand, within B158DRAFT_0746 at 749.135 kb on + strand, within B158DRAFT_0746 at 749.135 kb on + strand, within B158DRAFT_0746 at 749.143 kb on + strand, within B158DRAFT_0746 at 749.151 kb on - strand, within B158DRAFT_0746 at 749.153 kb on + strand, within B158DRAFT_0746 at 749.153 kb on + strand, within B158DRAFT_0746 at 749.153 kb on + strand, within B158DRAFT_0746 at 749.161 kb on - strand, within B158DRAFT_0746 at 749.170 kb on + strand, within B158DRAFT_0746 at 749.175 kb on + strand, within B158DRAFT_0746 at 749.188 kb on - strand, within B158DRAFT_0746 at 749.193 kb on - strand, within B158DRAFT_0746 at 749.213 kb on - strand, within B158DRAFT_0746 at 749.213 kb on - strand, within B158DRAFT_0746 at 749.229 kb on - strand, within B158DRAFT_0746 at 749.248 kb on + strand, within B158DRAFT_0746 at 749.314 kb on + strand, within B158DRAFT_0746 at 749.314 kb on + strand, within B158DRAFT_0746 at 749.322 kb on - strand, within B158DRAFT_0746 at 749.322 kb on - strand, within B158DRAFT_0746 at 749.322 kb on - strand, within B158DRAFT_0746
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 746,978 - B158DRAFT_0743 0.10 -0.4 747,380 - B158DRAFT_0743 0.73 +0.8 747,476 - B158DRAFT_0743 0.88 -1.6 747,476 - B158DRAFT_0743 0.88 -0.8 747,538 - +0.4 747,566 - -0.9 748,352 - -0.8 748,352 - +1.1 748,352 - -0.7 748,465 - B158DRAFT_0745 0.26 -0.3 748,478 - B158DRAFT_0745 0.29 -1.7 748,499 + B158DRAFT_0745 0.34 +0.4 748,502 - B158DRAFT_0745 0.35 +0.1 748,519 - B158DRAFT_0745 0.39 -1.7 748,535 - B158DRAFT_0745 0.43 -0.7 748,602 + B158DRAFT_0745 0.60 -1.0 748,782 + -1.3 748,782 + -1.0 748,782 + +0.5 748,782 + +1.4 748,790 - -0.7 748,830 + +0.0 748,830 - -1.3 748,874 + +3.8 748,957 + -2.3 749,016 + -0.8 749,016 + -0.8 749,016 + -0.4 749,024 - +0.0 749,024 - -0.8 749,086 + -1.7 749,094 + -1.0 749,094 - -1.8 749,135 + B158DRAFT_0746 0.11 -0.4 749,135 + B158DRAFT_0746 0.11 -0.5 749,135 + B158DRAFT_0746 0.11 +3.0 749,143 + B158DRAFT_0746 0.11 +0.3 749,151 - B158DRAFT_0746 0.12 -0.3 749,153 + B158DRAFT_0746 0.12 -0.7 749,153 + B158DRAFT_0746 0.12 +0.0 749,153 + B158DRAFT_0746 0.12 -0.2 749,161 - B158DRAFT_0746 0.12 -0.5 749,170 + B158DRAFT_0746 0.13 +0.3 749,175 + B158DRAFT_0746 0.13 +0.0 749,188 - B158DRAFT_0746 0.14 -1.6 749,193 - B158DRAFT_0746 0.14 +0.5 749,213 - B158DRAFT_0746 0.15 +1.8 749,213 - B158DRAFT_0746 0.15 -0.8 749,229 - B158DRAFT_0746 0.16 +1.3 749,248 + B158DRAFT_0746 0.17 -1.0 749,314 + B158DRAFT_0746 0.20 +0.6 749,314 + B158DRAFT_0746 0.20 -0.8 749,322 - B158DRAFT_0746 0.21 -1.8 749,322 - B158DRAFT_0746 0.21 -1.8 749,322 - B158DRAFT_0746 0.21 -0.8
Or see this region's nucleotide sequence