Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0677 and B158DRAFT_0678 are separated by 29 nucleotides B158DRAFT_0678 and B158DRAFT_0679 are separated by 67 nucleotides B158DRAFT_0679 and B158DRAFT_0680 are separated by 16 nucleotides B158DRAFT_0680 and B158DRAFT_0681 are separated by 68 nucleotides B158DRAFT_0681 and B158DRAFT_0682 are separated by 24 nucleotides
B158DRAFT_0677: B158DRAFT_0677 - Uncharacterized small membrane protein, at 679,914 to 680,294
_0677
B158DRAFT_0678: B158DRAFT_0678 - TIGR02444 family protein, at 680,324 to 680,830
_0678
B158DRAFT_0679: B158DRAFT_0679 - FKBP-type peptidyl-prolyl cis-trans isomerases 1, at 680,898 to 681,845
_0679
B158DRAFT_0680: B158DRAFT_0680 - Predicted membrane protein, at 681,862 to 682,188
_0680
B158DRAFT_0681: B158DRAFT_0681 - Uncharacterized enzyme involved in biosynthesis of extracellular polysaccharides, at 682,257 to 682,553
_0681
B158DRAFT_0682: B158DRAFT_0682 - Disulfide bond formation protein DsbB, at 682,578 to 683,090
_0682
Position (kb) 680
681
682 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 679.946 kb on + strand at 679.960 kb on + strand, within B158DRAFT_0677 at 679.965 kb on + strand, within B158DRAFT_0677 at 679.990 kb on + strand, within B158DRAFT_0677 at 679.990 kb on + strand, within B158DRAFT_0677 at 679.990 kb on + strand, within B158DRAFT_0677 at 679.990 kb on + strand, within B158DRAFT_0677 at 679.998 kb on - strand, within B158DRAFT_0677 at 680.011 kb on + strand, within B158DRAFT_0677 at 680.110 kb on - strand, within B158DRAFT_0677 at 680.110 kb on - strand, within B158DRAFT_0677 at 680.110 kb on - strand, within B158DRAFT_0677 at 680.163 kb on - strand, within B158DRAFT_0677 at 680.255 kb on - strand, within B158DRAFT_0677 at 680.255 kb on - strand, within B158DRAFT_0677 at 680.262 kb on + strand at 680.262 kb on + strand at 680.262 kb on + strand at 680.270 kb on - strand at 680.270 kb on - strand at 680.283 kb on - strand at 680.283 kb on - strand at 680.323 kb on - strand at 680.377 kb on - strand, within B158DRAFT_0678 at 680.409 kb on - strand, within B158DRAFT_0678 at 680.409 kb on - strand, within B158DRAFT_0678 at 680.423 kb on + strand, within B158DRAFT_0678 at 680.423 kb on + strand, within B158DRAFT_0678 at 680.441 kb on - strand, within B158DRAFT_0678 at 680.480 kb on - strand, within B158DRAFT_0678 at 680.480 kb on - strand, within B158DRAFT_0678 at 680.480 kb on - strand, within B158DRAFT_0678 at 680.488 kb on - strand, within B158DRAFT_0678 at 680.488 kb on - strand, within B158DRAFT_0678 at 680.531 kb on + strand, within B158DRAFT_0678 at 680.539 kb on - strand, within B158DRAFT_0678 at 680.539 kb on - strand, within B158DRAFT_0678 at 680.566 kb on - strand, within B158DRAFT_0678 at 680.666 kb on + strand, within B158DRAFT_0678 at 680.666 kb on + strand, within B158DRAFT_0678 at 680.666 kb on + strand, within B158DRAFT_0678 at 680.674 kb on - strand, within B158DRAFT_0678 at 680.674 kb on - strand, within B158DRAFT_0678 at 680.674 kb on - strand, within B158DRAFT_0678 at 680.674 kb on - strand, within B158DRAFT_0678 at 680.770 kb on + strand, within B158DRAFT_0678 at 680.771 kb on + strand, within B158DRAFT_0678 at 680.777 kb on + strand, within B158DRAFT_0678 at 680.800 kb on + strand at 680.803 kb on - strand at 680.818 kb on - strand at 680.824 kb on + strand at 680.824 kb on + strand at 680.824 kb on + strand at 680.824 kb on + strand at 680.824 kb on + strand at 680.824 kb on + strand at 680.824 kb on + strand at 680.824 kb on + strand at 680.824 kb on + strand at 680.824 kb on + strand at 680.832 kb on - strand at 680.832 kb on - strand at 680.832 kb on - strand at 680.832 kb on - strand at 680.832 kb on - strand at 680.851 kb on + strand at 680.921 kb on - strand at 680.944 kb on + strand at 680.944 kb on + strand at 680.947 kb on - strand at 680.952 kb on - strand at 680.959 kb on + strand at 680.998 kb on + strand, within B158DRAFT_0679 at 680.998 kb on + strand, within B158DRAFT_0679 at 681.006 kb on - strand, within B158DRAFT_0679 at 681.032 kb on - strand, within B158DRAFT_0679 at 681.094 kb on + strand, within B158DRAFT_0679 at 681.094 kb on + strand, within B158DRAFT_0679 at 681.134 kb on + strand, within B158DRAFT_0679 at 681.200 kb on + strand, within B158DRAFT_0679 at 681.210 kb on + strand, within B158DRAFT_0679 at 681.251 kb on - strand, within B158DRAFT_0679 at 681.301 kb on + strand, within B158DRAFT_0679 at 681.307 kb on - strand, within B158DRAFT_0679 at 681.365 kb on - strand, within B158DRAFT_0679 at 681.365 kb on - strand, within B158DRAFT_0679 at 681.365 kb on - strand, within B158DRAFT_0679 at 681.408 kb on + strand, within B158DRAFT_0679 at 681.416 kb on - strand, within B158DRAFT_0679 at 681.416 kb on - strand, within B158DRAFT_0679 at 681.497 kb on - strand, within B158DRAFT_0679 at 681.561 kb on + strand, within B158DRAFT_0679 at 681.580 kb on + strand, within B158DRAFT_0679 at 681.583 kb on - strand, within B158DRAFT_0679 at 681.588 kb on - strand, within B158DRAFT_0679 at 681.636 kb on - strand, within B158DRAFT_0679 at 681.686 kb on + strand, within B158DRAFT_0679 at 681.734 kb on - strand, within B158DRAFT_0679 at 681.748 kb on + strand, within B158DRAFT_0679 at 681.749 kb on - strand, within B158DRAFT_0679 at 681.756 kb on - strand at 681.781 kb on + strand at 681.783 kb on + strand at 681.795 kb on + strand at 682.008 kb on + strand, within B158DRAFT_0680 at 682.018 kb on + strand, within B158DRAFT_0680 at 682.056 kb on - strand, within B158DRAFT_0680 at 682.060 kb on - strand, within B158DRAFT_0680 at 682.098 kb on - strand, within B158DRAFT_0680 at 682.303 kb on + strand, within B158DRAFT_0681 at 682.335 kb on + strand, within B158DRAFT_0681 at 682.343 kb on - strand, within B158DRAFT_0681 at 682.357 kb on + strand, within B158DRAFT_0681 at 682.357 kb on + strand, within B158DRAFT_0681 at 682.372 kb on - strand, within B158DRAFT_0681 at 682.452 kb on - strand, within B158DRAFT_0681 at 682.570 kb on - strand at 682.575 kb on + strand at 682.611 kb on - strand at 682.611 kb on - strand at 682.611 kb on - strand at 682.611 kb on - strand at 682.611 kb on - strand at 682.621 kb on - strand at 682.626 kb on + strand at 682.634 kb on - strand, within B158DRAFT_0682 at 682.727 kb on - strand, within B158DRAFT_0682 at 682.770 kb on + strand, within B158DRAFT_0682 at 682.783 kb on + strand, within B158DRAFT_0682 at 682.783 kb on + strand, within B158DRAFT_0682 at 682.786 kb on - strand, within B158DRAFT_0682 at 682.788 kb on + strand, within B158DRAFT_0682
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 679,946 + +0.8 679,960 + B158DRAFT_0677 0.12 -0.2 679,965 + B158DRAFT_0677 0.13 -0.4 679,990 + B158DRAFT_0677 0.20 -1.7 679,990 + B158DRAFT_0677 0.20 -0.6 679,990 + B158DRAFT_0677 0.20 -0.9 679,990 + B158DRAFT_0677 0.20 -2.9 679,998 - B158DRAFT_0677 0.22 +0.0 680,011 + B158DRAFT_0677 0.25 -0.9 680,110 - B158DRAFT_0677 0.51 -0.4 680,110 - B158DRAFT_0677 0.51 +1.2 680,110 - B158DRAFT_0677 0.51 -0.5 680,163 - B158DRAFT_0677 0.65 +1.1 680,255 - B158DRAFT_0677 0.90 +4.6 680,255 - B158DRAFT_0677 0.90 +4.8 680,262 + -0.8 680,262 + +0.7 680,262 + +1.4 680,270 - +3.8 680,270 - +4.1 680,283 - +4.1 680,283 - +3.2 680,323 - +4.3 680,377 - B158DRAFT_0678 0.10 +4.8 680,409 - B158DRAFT_0678 0.17 +5.4 680,409 - B158DRAFT_0678 0.17 +5.1 680,423 + B158DRAFT_0678 0.20 +5.0 680,423 + B158DRAFT_0678 0.20 +5.0 680,441 - B158DRAFT_0678 0.23 +5.6 680,480 - B158DRAFT_0678 0.31 +4.8 680,480 - B158DRAFT_0678 0.31 +3.1 680,480 - B158DRAFT_0678 0.31 +4.9 680,488 - B158DRAFT_0678 0.32 +5.2 680,488 - B158DRAFT_0678 0.32 +4.8 680,531 + B158DRAFT_0678 0.41 +5.1 680,539 - B158DRAFT_0678 0.42 +4.7 680,539 - B158DRAFT_0678 0.42 +4.1 680,566 - B158DRAFT_0678 0.48 +4.3 680,666 + B158DRAFT_0678 0.67 +4.7 680,666 + B158DRAFT_0678 0.67 +5.5 680,666 + B158DRAFT_0678 0.67 +5.2 680,674 - B158DRAFT_0678 0.69 +4.6 680,674 - B158DRAFT_0678 0.69 +10.3 680,674 - B158DRAFT_0678 0.69 +5.1 680,674 - B158DRAFT_0678 0.69 +5.9 680,770 + B158DRAFT_0678 0.88 +1.7 680,771 + B158DRAFT_0678 0.88 -0.2 680,777 + B158DRAFT_0678 0.89 +0.0 680,800 + -0.8 680,803 - -0.5 680,818 - -0.6 680,824 + -0.1 680,824 + -1.1 680,824 + -1.1 680,824 + -0.2 680,824 + +5.4 680,824 + +1.1 680,824 + -1.3 680,824 + -1.9 680,824 + +1.2 680,824 + -0.7 680,832 - -0.1 680,832 - -0.7 680,832 - -1.9 680,832 - +1.0 680,832 - -0.2 680,851 + +0.2 680,921 - -1.5 680,944 + -0.8 680,944 + -1.0 680,947 - -1.1 680,952 - -1.5 680,959 + -0.4 680,998 + B158DRAFT_0679 0.11 -1.4 680,998 + B158DRAFT_0679 0.11 -1.1 681,006 - B158DRAFT_0679 0.11 +0.2 681,032 - B158DRAFT_0679 0.14 +0.6 681,094 + B158DRAFT_0679 0.21 -1.0 681,094 + B158DRAFT_0679 0.21 +0.7 681,134 + B158DRAFT_0679 0.25 -2.4 681,200 + B158DRAFT_0679 0.32 -1.9 681,210 + B158DRAFT_0679 0.33 -1.3 681,251 - B158DRAFT_0679 0.37 -0.2 681,301 + B158DRAFT_0679 0.43 +1.2 681,307 - B158DRAFT_0679 0.43 -0.9 681,365 - B158DRAFT_0679 0.49 -1.3 681,365 - B158DRAFT_0679 0.49 -4.2 681,365 - B158DRAFT_0679 0.49 -0.4 681,408 + B158DRAFT_0679 0.54 -1.2 681,416 - B158DRAFT_0679 0.55 -0.9 681,416 - B158DRAFT_0679 0.55 -0.9 681,497 - B158DRAFT_0679 0.63 +1.0 681,561 + B158DRAFT_0679 0.70 -1.1 681,580 + B158DRAFT_0679 0.72 +0.7 681,583 - B158DRAFT_0679 0.72 -0.8 681,588 - B158DRAFT_0679 0.73 +0.4 681,636 - B158DRAFT_0679 0.78 +0.2 681,686 + B158DRAFT_0679 0.83 -1.2 681,734 - B158DRAFT_0679 0.88 -0.4 681,748 + B158DRAFT_0679 0.90 -0.3 681,749 - B158DRAFT_0679 0.90 -1.4 681,756 - +2.7 681,781 + -1.1 681,783 + -0.7 681,795 + -0.2 682,008 + B158DRAFT_0680 0.45 -1.7 682,018 + B158DRAFT_0680 0.48 -0.6 682,056 - B158DRAFT_0680 0.59 -0.8 682,060 - B158DRAFT_0680 0.61 -0.4 682,098 - B158DRAFT_0680 0.72 -1.1 682,303 + B158DRAFT_0681 0.15 +6.4 682,335 + B158DRAFT_0681 0.26 +6.3 682,343 - B158DRAFT_0681 0.29 +5.9 682,357 + B158DRAFT_0681 0.34 +5.2 682,357 + B158DRAFT_0681 0.34 +6.8 682,372 - B158DRAFT_0681 0.39 +6.4 682,452 - B158DRAFT_0681 0.66 +6.1 682,570 - +4.4 682,575 + +5.9 682,611 - +2.6 682,611 - -0.6 682,611 - +1.9 682,611 - +1.7 682,611 - -0.3 682,621 - +1.7 682,626 + +5.6 682,634 - B158DRAFT_0682 0.11 -1.8 682,727 - B158DRAFT_0682 0.29 +1.1 682,770 + B158DRAFT_0682 0.37 +0.3 682,783 + B158DRAFT_0682 0.40 +1.4 682,783 + B158DRAFT_0682 0.40 -1.8 682,786 - B158DRAFT_0682 0.41 +0.7 682,788 + B158DRAFT_0682 0.41 +1.9
Or see this region's nucleotide sequence