Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0357 and B158DRAFT_0358 are separated by 9 nucleotides B158DRAFT_0358 and B158DRAFT_0359 overlap by 7 nucleotides B158DRAFT_0359 and B158DRAFT_0360 are separated by 14 nucleotides B158DRAFT_0360 and B158DRAFT_0361 are separated by 48 nucleotides
B158DRAFT_0357: B158DRAFT_0357 - Xaa-Pro aminopeptidase, at 359,889 to 361,208
_0357
B158DRAFT_0358: B158DRAFT_0358 - uncharacterized protein, YigZ family, at 361,218 to 361,871
_0358
B158DRAFT_0359: B158DRAFT_0359 - Uncharacterized protein conserved in bacteria, at 361,865 to 362,230
_0359
B158DRAFT_0360: B158DRAFT_0360 - Uncharacterized protein conserved in bacteria, at 362,245 to 362,487
_0360
B158DRAFT_0361: B158DRAFT_0361 - Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 362,536 to 364,515
_0361
Position (kb)
361
362
363 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 360.886 kb on + strand, within B158DRAFT_0357 at 360.894 kb on - strand, within B158DRAFT_0357 at 360.894 kb on - strand, within B158DRAFT_0357 at 360.904 kb on - strand, within B158DRAFT_0357 at 360.912 kb on - strand, within B158DRAFT_0357 at 360.930 kb on + strand, within B158DRAFT_0357 at 361.018 kb on - strand, within B158DRAFT_0357 at 361.043 kb on - strand, within B158DRAFT_0357 at 361.064 kb on - strand, within B158DRAFT_0357 at 361.118 kb on + strand at 361.118 kb on + strand at 361.118 kb on + strand at 361.126 kb on - strand at 361.126 kb on - strand at 361.150 kb on + strand at 361.155 kb on + strand at 361.173 kb on + strand at 361.197 kb on + strand at 361.228 kb on + strand at 361.228 kb on + strand at 361.236 kb on - strand at 361.260 kb on + strand at 361.268 kb on - strand at 361.268 kb on - strand at 361.268 kb on - strand at 361.270 kb on + strand at 361.273 kb on - strand at 361.278 kb on - strand at 361.332 kb on + strand, within B158DRAFT_0358 at 361.335 kb on - strand, within B158DRAFT_0358 at 361.341 kb on - strand, within B158DRAFT_0358 at 361.383 kb on - strand, within B158DRAFT_0358 at 361.397 kb on - strand, within B158DRAFT_0358 at 361.431 kb on + strand, within B158DRAFT_0358 at 361.468 kb on + strand, within B158DRAFT_0358 at 361.520 kb on - strand, within B158DRAFT_0358 at 361.580 kb on + strand, within B158DRAFT_0358 at 361.590 kb on - strand, within B158DRAFT_0358 at 361.593 kb on + strand, within B158DRAFT_0358 at 361.595 kb on - strand, within B158DRAFT_0358 at 361.598 kb on + strand, within B158DRAFT_0358 at 361.715 kb on + strand, within B158DRAFT_0358 at 361.715 kb on + strand, within B158DRAFT_0358 at 361.782 kb on - strand, within B158DRAFT_0358 at 361.785 kb on + strand, within B158DRAFT_0358 at 361.785 kb on + strand, within B158DRAFT_0358 at 361.793 kb on - strand, within B158DRAFT_0358 at 361.793 kb on - strand, within B158DRAFT_0358 at 361.842 kb on - strand at 362.062 kb on - strand, within B158DRAFT_0359 at 362.382 kb on + strand, within B158DRAFT_0360 at 362.560 kb on - strand at 362.574 kb on - strand at 362.643 kb on + strand at 362.651 kb on - strand at 362.740 kb on + strand, within B158DRAFT_0361 at 362.748 kb on - strand, within B158DRAFT_0361 at 362.748 kb on - strand, within B158DRAFT_0361 at 362.748 kb on - strand, within B158DRAFT_0361
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 360,886 + B158DRAFT_0357 0.76 -0.6 360,894 - B158DRAFT_0357 0.76 +1.6 360,894 - B158DRAFT_0357 0.76 -2.0 360,904 - B158DRAFT_0357 0.77 -0.7 360,912 - B158DRAFT_0357 0.78 +2.1 360,930 + B158DRAFT_0357 0.79 +0.8 361,018 - B158DRAFT_0357 0.86 -1.0 361,043 - B158DRAFT_0357 0.87 +0.9 361,064 - B158DRAFT_0357 0.89 +0.4 361,118 + -0.8 361,118 + +1.2 361,118 + -0.7 361,126 - -1.0 361,126 - +0.4 361,150 + +0.9 361,155 + -0.1 361,173 + +2.8 361,197 + -0.4 361,228 + -0.8 361,228 + -0.5 361,236 - +0.2 361,260 + +0.6 361,268 - -0.8 361,268 - +1.6 361,268 - -0.8 361,270 + -0.9 361,273 - +1.2 361,278 - +0.5 361,332 + B158DRAFT_0358 0.17 -0.4 361,335 - B158DRAFT_0358 0.18 -0.7 361,341 - B158DRAFT_0358 0.19 -1.6 361,383 - B158DRAFT_0358 0.25 +0.6 361,397 - B158DRAFT_0358 0.27 -0.4 361,431 + B158DRAFT_0358 0.33 -0.2 361,468 + B158DRAFT_0358 0.38 +5.3 361,520 - B158DRAFT_0358 0.46 -0.6 361,580 + B158DRAFT_0358 0.55 -1.1 361,590 - B158DRAFT_0358 0.57 +1.1 361,593 + B158DRAFT_0358 0.57 -0.7 361,595 - B158DRAFT_0358 0.58 +1.3 361,598 + B158DRAFT_0358 0.58 -1.2 361,715 + B158DRAFT_0358 0.76 +1.1 361,715 + B158DRAFT_0358 0.76 -0.9 361,782 - B158DRAFT_0358 0.86 +1.2 361,785 + B158DRAFT_0358 0.87 -0.9 361,785 + B158DRAFT_0358 0.87 -0.1 361,793 - B158DRAFT_0358 0.88 -0.3 361,793 - B158DRAFT_0358 0.88 -2.2 361,842 - -0.5 362,062 - B158DRAFT_0359 0.54 -0.5 362,382 + B158DRAFT_0360 0.56 -3.2 362,560 - -0.1 362,574 - -1.1 362,643 + -1.6 362,651 - +1.2 362,740 + B158DRAFT_0361 0.10 +0.1 362,748 - B158DRAFT_0361 0.11 -0.2 362,748 - B158DRAFT_0361 0.11 +0.5 362,748 - B158DRAFT_0361 0.11 +1.4
Or see this region's nucleotide sequence