Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0295 and B158DRAFT_0296 are separated by 394 nucleotides B158DRAFT_0296 and B158DRAFT_0297 are separated by 606 nucleotides B158DRAFT_0297 and B158DRAFT_0298 are separated by 2 nucleotides
B158DRAFT_0295: B158DRAFT_0295 - hydroxymethylpyrimidine synthase, at 297,562 to 299,445
_0295
B158DRAFT_0296: B158DRAFT_0296 - Uncharacterized protein conserved in bacteria, at 299,840 to 300,304
_0296
B158DRAFT_0297: B158DRAFT_0297 - Uncharacterized conserved protein, at 300,911 to 301,297
_0297
B158DRAFT_0298: B158DRAFT_0298 - Cysteine synthase, at 301,300 to 302,352
_0298
Position (kb)
299
300
301 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 298.931 kb on - strand, within B158DRAFT_0295 at 299.014 kb on + strand, within B158DRAFT_0295 at 299.054 kb on + strand, within B158DRAFT_0295 at 299.054 kb on + strand, within B158DRAFT_0295 at 299.062 kb on - strand, within B158DRAFT_0295 at 299.062 kb on - strand, within B158DRAFT_0295 at 299.067 kb on + strand, within B158DRAFT_0295 at 299.075 kb on - strand, within B158DRAFT_0295 at 299.075 kb on - strand, within B158DRAFT_0295 at 299.131 kb on - strand, within B158DRAFT_0295 at 299.164 kb on + strand, within B158DRAFT_0295 at 299.186 kb on - strand, within B158DRAFT_0295 at 299.191 kb on + strand, within B158DRAFT_0295 at 299.199 kb on - strand, within B158DRAFT_0295 at 299.199 kb on - strand, within B158DRAFT_0295 at 299.199 kb on - strand, within B158DRAFT_0295 at 299.233 kb on - strand, within B158DRAFT_0295 at 299.237 kb on + strand, within B158DRAFT_0295 at 299.238 kb on + strand, within B158DRAFT_0295 at 299.238 kb on + strand, within B158DRAFT_0295 at 299.238 kb on + strand, within B158DRAFT_0295 at 299.238 kb on + strand, within B158DRAFT_0295 at 299.246 kb on - strand, within B158DRAFT_0295 at 299.246 kb on - strand, within B158DRAFT_0295 at 299.246 kb on - strand, within B158DRAFT_0295 at 299.248 kb on + strand, within B158DRAFT_0295 at 299.250 kb on + strand, within B158DRAFT_0295 at 299.250 kb on + strand, within B158DRAFT_0295 at 299.254 kb on - strand, within B158DRAFT_0295 at 299.256 kb on - strand, within B158DRAFT_0295 at 299.256 kb on - strand, within B158DRAFT_0295 at 299.256 kb on - strand, within B158DRAFT_0295 at 299.262 kb on + strand at 299.316 kb on + strand at 299.358 kb on - strand at 299.359 kb on - strand at 299.366 kb on - strand at 299.462 kb on + strand at 299.523 kb on + strand at 299.528 kb on + strand at 299.528 kb on + strand at 299.561 kb on - strand at 299.630 kb on + strand at 299.711 kb on - strand at 299.711 kb on - strand at 299.756 kb on + strand at 299.759 kb on + strand at 299.845 kb on + strand at 299.878 kb on - strand at 299.878 kb on - strand at 299.878 kb on - strand at 299.882 kb on + strand at 299.882 kb on + strand at 299.941 kb on - strand, within B158DRAFT_0296 at 299.965 kb on + strand, within B158DRAFT_0296 at 299.978 kb on + strand, within B158DRAFT_0296 at 299.986 kb on - strand, within B158DRAFT_0296 at 299.986 kb on - strand, within B158DRAFT_0296 at 299.986 kb on - strand, within B158DRAFT_0296 at 299.991 kb on - strand, within B158DRAFT_0296 at 300.064 kb on - strand, within B158DRAFT_0296 at 300.105 kb on - strand, within B158DRAFT_0296 at 300.185 kb on + strand, within B158DRAFT_0296 at 300.185 kb on + strand, within B158DRAFT_0296 at 300.188 kb on - strand, within B158DRAFT_0296 at 300.190 kb on + strand, within B158DRAFT_0296 at 300.190 kb on + strand, within B158DRAFT_0296 at 300.190 kb on + strand, within B158DRAFT_0296 at 300.195 kb on + strand, within B158DRAFT_0296 at 300.198 kb on - strand, within B158DRAFT_0296 at 300.209 kb on + strand, within B158DRAFT_0296 at 300.250 kb on + strand, within B158DRAFT_0296 at 300.250 kb on + strand, within B158DRAFT_0296 at 300.253 kb on - strand, within B158DRAFT_0296 at 300.263 kb on - strand at 300.263 kb on - strand at 300.265 kb on + strand at 301.085 kb on + strand, within B158DRAFT_0297 at 301.156 kb on + strand, within B158DRAFT_0297 at 301.156 kb on + strand, within B158DRAFT_0297 at 301.218 kb on + strand, within B158DRAFT_0297
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 298,931 - B158DRAFT_0295 0.73 -1.0 299,014 + B158DRAFT_0295 0.77 -0.5 299,054 + B158DRAFT_0295 0.79 -0.5 299,054 + B158DRAFT_0295 0.79 +2.2 299,062 - B158DRAFT_0295 0.80 +1.2 299,062 - B158DRAFT_0295 0.80 -0.4 299,067 + B158DRAFT_0295 0.80 +2.9 299,075 - B158DRAFT_0295 0.80 +0.6 299,075 - B158DRAFT_0295 0.80 +1.6 299,131 - B158DRAFT_0295 0.83 +3.5 299,164 + B158DRAFT_0295 0.85 +2.5 299,186 - B158DRAFT_0295 0.86 -0.5 299,191 + B158DRAFT_0295 0.86 +0.2 299,199 - B158DRAFT_0295 0.87 +1.8 299,199 - B158DRAFT_0295 0.87 +1.4 299,199 - B158DRAFT_0295 0.87 -1.5 299,233 - B158DRAFT_0295 0.89 +5.7 299,237 + B158DRAFT_0295 0.89 +1.4 299,238 + B158DRAFT_0295 0.89 -0.1 299,238 + B158DRAFT_0295 0.89 -2.4 299,238 + B158DRAFT_0295 0.89 -0.4 299,238 + B158DRAFT_0295 0.89 -0.8 299,246 - B158DRAFT_0295 0.89 +1.0 299,246 - B158DRAFT_0295 0.89 +0.1 299,246 - B158DRAFT_0295 0.89 +3.8 299,248 + B158DRAFT_0295 0.89 -0.8 299,250 + B158DRAFT_0295 0.90 -0.5 299,250 + B158DRAFT_0295 0.90 +2.0 299,254 - B158DRAFT_0295 0.90 -0.0 299,256 - B158DRAFT_0295 0.90 +1.2 299,256 - B158DRAFT_0295 0.90 +0.0 299,256 - B158DRAFT_0295 0.90 -1.5 299,262 + -1.0 299,316 + -0.3 299,358 - -0.0 299,359 - +0.7 299,366 - +1.2 299,462 + -0.2 299,523 + +1.2 299,528 + +0.1 299,528 + -1.2 299,561 - +0.4 299,630 + +1.0 299,711 - -0.8 299,711 - +0.4 299,756 + -0.1 299,759 + -1.6 299,845 + -0.4 299,878 - -1.1 299,878 - +0.1 299,878 - +0.4 299,882 + -1.7 299,882 + +0.1 299,941 - B158DRAFT_0296 0.22 -1.4 299,965 + B158DRAFT_0296 0.27 -0.3 299,978 + B158DRAFT_0296 0.30 -2.1 299,986 - B158DRAFT_0296 0.31 -0.4 299,986 - B158DRAFT_0296 0.31 -1.9 299,986 - B158DRAFT_0296 0.31 +0.1 299,991 - B158DRAFT_0296 0.32 -1.8 300,064 - B158DRAFT_0296 0.48 +0.3 300,105 - B158DRAFT_0296 0.57 -0.4 300,185 + B158DRAFT_0296 0.74 +1.2 300,185 + B158DRAFT_0296 0.74 +0.1 300,188 - B158DRAFT_0296 0.75 +0.2 300,190 + B158DRAFT_0296 0.75 -0.2 300,190 + B158DRAFT_0296 0.75 +1.4 300,190 + B158DRAFT_0296 0.75 +1.0 300,195 + B158DRAFT_0296 0.76 +0.0 300,198 - B158DRAFT_0296 0.77 -2.7 300,209 + B158DRAFT_0296 0.79 -0.1 300,250 + B158DRAFT_0296 0.88 -1.1 300,250 + B158DRAFT_0296 0.88 -1.1 300,253 - B158DRAFT_0296 0.89 +1.9 300,263 - +1.9 300,263 - +0.5 300,265 + +0.4 301,085 + B158DRAFT_0297 0.45 -0.7 301,156 + B158DRAFT_0297 0.63 -0.8 301,156 + B158DRAFT_0297 0.63 -1.0 301,218 + B158DRAFT_0297 0.79 -0.5
Or see this region's nucleotide sequence