Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0262 and B158DRAFT_0263 are separated by 98 nucleotides B158DRAFT_0263 and B158DRAFT_0264 are separated by 128 nucleotides
B158DRAFT_0262: B158DRAFT_0262 - hypothetical protein, at 268,544 to 269,149
_0262
B158DRAFT_0263: B158DRAFT_0263 - Predicted redox protein, regulator of disulfide bond formation, at 269,248 to 269,646
_0263
B158DRAFT_0264: B158DRAFT_0264 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 269,775 to 270,410
_0264
Position (kb)
269
270 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 268.331 kb on + strand at 268.331 kb on + strand at 268.331 kb on + strand at 268.339 kb on - strand at 268.358 kb on + strand at 268.358 kb on + strand at 268.364 kb on + strand at 268.371 kb on - strand at 268.456 kb on + strand at 268.466 kb on + strand at 268.469 kb on - strand at 268.469 kb on - strand at 268.474 kb on - strand at 268.474 kb on - strand at 268.507 kb on + strand at 268.536 kb on - strand at 268.614 kb on + strand, within B158DRAFT_0262 at 268.615 kb on - strand, within B158DRAFT_0262 at 268.615 kb on - strand, within B158DRAFT_0262 at 268.622 kb on - strand, within B158DRAFT_0262 at 268.632 kb on - strand, within B158DRAFT_0262 at 268.646 kb on + strand, within B158DRAFT_0262 at 268.646 kb on + strand, within B158DRAFT_0262 at 268.679 kb on + strand, within B158DRAFT_0262 at 268.705 kb on + strand, within B158DRAFT_0262 at 268.710 kb on + strand, within B158DRAFT_0262 at 268.713 kb on - strand, within B158DRAFT_0262 at 268.713 kb on - strand, within B158DRAFT_0262 at 268.713 kb on - strand, within B158DRAFT_0262 at 268.713 kb on - strand, within B158DRAFT_0262 at 268.713 kb on - strand, within B158DRAFT_0262 at 268.713 kb on - strand, within B158DRAFT_0262 at 268.804 kb on - strand, within B158DRAFT_0262 at 268.829 kb on + strand, within B158DRAFT_0262 at 268.887 kb on + strand, within B158DRAFT_0262 at 268.887 kb on + strand, within B158DRAFT_0262 at 268.918 kb on + strand, within B158DRAFT_0262 at 268.918 kb on + strand, within B158DRAFT_0262 at 268.918 kb on + strand, within B158DRAFT_0262 at 268.926 kb on - strand, within B158DRAFT_0262 at 268.926 kb on - strand, within B158DRAFT_0262 at 268.926 kb on - strand, within B158DRAFT_0262 at 269.017 kb on + strand, within B158DRAFT_0262 at 269.022 kb on + strand, within B158DRAFT_0262 at 269.030 kb on - strand, within B158DRAFT_0262 at 269.030 kb on - strand, within B158DRAFT_0262 at 269.035 kb on - strand, within B158DRAFT_0262 at 269.187 kb on + strand at 269.187 kb on + strand at 269.325 kb on - strand, within B158DRAFT_0263 at 269.430 kb on - strand, within B158DRAFT_0263 at 269.528 kb on - strand, within B158DRAFT_0263 at 269.619 kb on + strand at 269.629 kb on + strand at 269.636 kb on - strand at 269.636 kb on - strand at 269.637 kb on - strand at 269.637 kb on - strand at 269.723 kb on - strand at 269.791 kb on - strand at 269.801 kb on + strand at 270.028 kb on - strand, within B158DRAFT_0264 at 270.030 kb on + strand, within B158DRAFT_0264 at 270.030 kb on + strand, within B158DRAFT_0264 at 270.038 kb on - strand, within B158DRAFT_0264 at 270.150 kb on - strand, within B158DRAFT_0264 at 270.259 kb on - strand, within B158DRAFT_0264 at 270.264 kb on - strand, within B158DRAFT_0264 at 270.272 kb on + strand, within B158DRAFT_0264 at 270.272 kb on + strand, within B158DRAFT_0264 at 270.272 kb on + strand, within B158DRAFT_0264 at 270.280 kb on - strand, within B158DRAFT_0264 at 270.280 kb on - strand, within B158DRAFT_0264 at 270.334 kb on - strand, within B158DRAFT_0264
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 268,331 + -0.1 268,331 + +0.0 268,331 + +0.3 268,339 - -0.5 268,358 + -0.9 268,358 + -1.4 268,364 + -0.7 268,371 - -1.4 268,456 + -1.0 268,466 + +0.9 268,469 - +1.7 268,469 - -2.3 268,474 - -1.0 268,474 - -1.0 268,507 + -0.4 268,536 - -2.1 268,614 + B158DRAFT_0262 0.12 +3.1 268,615 - B158DRAFT_0262 0.12 -0.2 268,615 - B158DRAFT_0262 0.12 -3.0 268,622 - B158DRAFT_0262 0.13 -2.5 268,632 - B158DRAFT_0262 0.15 -1.2 268,646 + B158DRAFT_0262 0.17 +1.8 268,646 + B158DRAFT_0262 0.17 +0.8 268,679 + B158DRAFT_0262 0.22 -1.8 268,705 + B158DRAFT_0262 0.27 -0.7 268,710 + B158DRAFT_0262 0.27 +0.6 268,713 - B158DRAFT_0262 0.28 -1.3 268,713 - B158DRAFT_0262 0.28 +0.5 268,713 - B158DRAFT_0262 0.28 +0.2 268,713 - B158DRAFT_0262 0.28 +0.0 268,713 - B158DRAFT_0262 0.28 +0.2 268,713 - B158DRAFT_0262 0.28 -0.1 268,804 - B158DRAFT_0262 0.43 +2.0 268,829 + B158DRAFT_0262 0.47 +1.5 268,887 + B158DRAFT_0262 0.57 -1.2 268,887 + B158DRAFT_0262 0.57 -1.1 268,918 + B158DRAFT_0262 0.62 +0.0 268,918 + B158DRAFT_0262 0.62 -2.4 268,918 + B158DRAFT_0262 0.62 -0.0 268,926 - B158DRAFT_0262 0.63 -0.8 268,926 - B158DRAFT_0262 0.63 -1.0 268,926 - B158DRAFT_0262 0.63 +0.2 269,017 + B158DRAFT_0262 0.78 -0.6 269,022 + B158DRAFT_0262 0.79 +1.0 269,030 - B158DRAFT_0262 0.80 -0.6 269,030 - B158DRAFT_0262 0.80 -0.2 269,035 - B158DRAFT_0262 0.81 -0.1 269,187 + -1.2 269,187 + -2.4 269,325 - B158DRAFT_0263 0.19 +0.1 269,430 - B158DRAFT_0263 0.46 -1.4 269,528 - B158DRAFT_0263 0.70 +1.8 269,619 + -1.9 269,629 + -1.2 269,636 - -0.2 269,636 - +0.1 269,637 - +0.4 269,637 - +1.0 269,723 - +0.3 269,791 - -1.1 269,801 + +0.6 270,028 - B158DRAFT_0264 0.40 -0.6 270,030 + B158DRAFT_0264 0.40 -0.0 270,030 + B158DRAFT_0264 0.40 -1.7 270,038 - B158DRAFT_0264 0.41 +1.4 270,150 - B158DRAFT_0264 0.59 -1.4 270,259 - B158DRAFT_0264 0.76 +1.3 270,264 - B158DRAFT_0264 0.77 +0.6 270,272 + B158DRAFT_0264 0.78 -1.0 270,272 + B158DRAFT_0264 0.78 -0.6 270,272 + B158DRAFT_0264 0.78 +2.0 270,280 - B158DRAFT_0264 0.79 -1.0 270,280 - B158DRAFT_0264 0.79 -1.1 270,334 - B158DRAFT_0264 0.88 +2.3
Or see this region's nucleotide sequence