Experiment: m.b. Sisomicin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_0258 and B158DRAFT_0259 are separated by 9 nucleotides B158DRAFT_0259 and B158DRAFT_0260 are separated by 26 nucleotides B158DRAFT_0260 and B158DRAFT_0261 are separated by 58 nucleotides
B158DRAFT_0258: B158DRAFT_0258 - transporter, NhaC family (TC 2.A.35), at 263,246 to 264,598
_0258
B158DRAFT_0259: B158DRAFT_0259 - shikimate 5-dehydrogenase, at 264,608 to 265,402
_0259
B158DRAFT_0260: B158DRAFT_0260 - Putative protein-S-isoprenylcysteine methyltransferase, at 265,429 to 265,890
_0260
B158DRAFT_0261: B158DRAFT_0261 - catalase/peroxidase HPI, at 265,949 to 268,180
_0261
Position (kb)
264
265
266 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 263.784 kb on - strand, within B158DRAFT_0258 at 263.819 kb on - strand, within B158DRAFT_0258 at 263.848 kb on + strand, within B158DRAFT_0258 at 263.848 kb on + strand, within B158DRAFT_0258 at 263.856 kb on - strand, within B158DRAFT_0258 at 263.880 kb on - strand, within B158DRAFT_0258 at 264.164 kb on + strand, within B158DRAFT_0258 at 264.164 kb on + strand, within B158DRAFT_0258 at 264.172 kb on - strand, within B158DRAFT_0258 at 264.172 kb on - strand, within B158DRAFT_0258 at 264.188 kb on - strand, within B158DRAFT_0258 at 264.200 kb on + strand, within B158DRAFT_0258 at 264.284 kb on - strand, within B158DRAFT_0258 at 264.284 kb on - strand, within B158DRAFT_0258 at 264.345 kb on + strand, within B158DRAFT_0258 at 264.430 kb on - strand, within B158DRAFT_0258 at 264.517 kb on + strand at 264.534 kb on + strand at 264.569 kb on - strand at 264.650 kb on - strand at 264.661 kb on + strand at 264.747 kb on - strand, within B158DRAFT_0259 at 264.804 kb on + strand, within B158DRAFT_0259 at 264.812 kb on - strand, within B158DRAFT_0259 at 264.838 kb on - strand, within B158DRAFT_0259 at 264.844 kb on - strand, within B158DRAFT_0259 at 264.852 kb on - strand, within B158DRAFT_0259 at 264.855 kb on + strand, within B158DRAFT_0259 at 264.863 kb on - strand, within B158DRAFT_0259 at 264.863 kb on - strand, within B158DRAFT_0259 at 264.894 kb on - strand, within B158DRAFT_0259 at 264.939 kb on + strand, within B158DRAFT_0259 at 264.947 kb on - strand, within B158DRAFT_0259 at 264.956 kb on + strand, within B158DRAFT_0259 at 264.998 kb on + strand, within B158DRAFT_0259 at 264.998 kb on + strand, within B158DRAFT_0259 at 264.998 kb on + strand, within B158DRAFT_0259 at 264.998 kb on + strand, within B158DRAFT_0259 at 264.998 kb on + strand, within B158DRAFT_0259 at 264.998 kb on + strand, within B158DRAFT_0259 at 264.998 kb on + strand, within B158DRAFT_0259 at 264.998 kb on + strand, within B158DRAFT_0259 at 265.006 kb on - strand, within B158DRAFT_0259 at 265.006 kb on - strand, within B158DRAFT_0259 at 265.006 kb on - strand, within B158DRAFT_0259 at 265.006 kb on - strand, within B158DRAFT_0259 at 265.017 kb on + strand, within B158DRAFT_0259 at 265.034 kb on + strand, within B158DRAFT_0259 at 265.056 kb on + strand, within B158DRAFT_0259 at 265.056 kb on + strand, within B158DRAFT_0259 at 265.064 kb on - strand, within B158DRAFT_0259 at 265.064 kb on - strand, within B158DRAFT_0259 at 265.064 kb on - strand, within B158DRAFT_0259 at 265.064 kb on - strand, within B158DRAFT_0259 at 265.080 kb on - strand, within B158DRAFT_0259 at 265.291 kb on + strand, within B158DRAFT_0259 at 265.299 kb on - strand, within B158DRAFT_0259 at 265.316 kb on + strand, within B158DRAFT_0259 at 265.350 kb on + strand at 265.351 kb on - strand at 265.358 kb on - strand at 265.493 kb on + strand, within B158DRAFT_0260 at 265.501 kb on - strand, within B158DRAFT_0260 at 265.521 kb on + strand, within B158DRAFT_0260 at 265.576 kb on - strand, within B158DRAFT_0260 at 265.623 kb on - strand, within B158DRAFT_0260 at 265.832 kb on - strand, within B158DRAFT_0260 at 265.839 kb on + strand, within B158DRAFT_0260 at 266.044 kb on + strand at 266.129 kb on + strand at 266.129 kb on + strand at 266.129 kb on + strand at 266.129 kb on + strand at 266.129 kb on + strand at 266.129 kb on + strand at 266.129 kb on + strand at 266.129 kb on + strand at 266.129 kb on + strand at 266.129 kb on + strand at 266.136 kb on + strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.137 kb on - strand at 266.144 kb on - strand at 266.213 kb on + strand, within B158DRAFT_0261 at 266.221 kb on - strand, within B158DRAFT_0261 at 266.228 kb on - strand, within B158DRAFT_0261 at 266.228 kb on - strand, within B158DRAFT_0261 at 266.228 kb on - strand, within B158DRAFT_0261 at 266.248 kb on + strand, within B158DRAFT_0261 at 266.256 kb on - strand, within B158DRAFT_0261 at 266.264 kb on + strand, within B158DRAFT_0261 at 266.264 kb on + strand, within B158DRAFT_0261 at 266.269 kb on + strand, within B158DRAFT_0261 at 266.320 kb on + strand, within B158DRAFT_0261 at 266.320 kb on + strand, within B158DRAFT_0261 at 266.320 kb on + strand, within B158DRAFT_0261 at 266.320 kb on + strand, within B158DRAFT_0261 at 266.320 kb on + strand, within B158DRAFT_0261 at 266.320 kb on + strand, within B158DRAFT_0261 at 266.320 kb on + strand, within B158DRAFT_0261 at 266.320 kb on + strand, within B158DRAFT_0261 at 266.328 kb on + strand, within B158DRAFT_0261 at 266.328 kb on - strand, within B158DRAFT_0261 at 266.328 kb on - strand, within B158DRAFT_0261 at 266.328 kb on - strand, within B158DRAFT_0261 at 266.328 kb on - strand, within B158DRAFT_0261 at 266.328 kb on - strand, within B158DRAFT_0261 at 266.351 kb on - strand, within B158DRAFT_0261 at 266.351 kb on - strand, within B158DRAFT_0261 at 266.351 kb on - strand, within B158DRAFT_0261
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.025 mg/ml remove 263,784 - B158DRAFT_0258 0.40 +1.2 263,819 - B158DRAFT_0258 0.42 +0.3 263,848 + B158DRAFT_0258 0.44 -1.3 263,848 + B158DRAFT_0258 0.44 +0.4 263,856 - B158DRAFT_0258 0.45 -0.2 263,880 - B158DRAFT_0258 0.47 +1.7 264,164 + B158DRAFT_0258 0.68 +1.4 264,164 + B158DRAFT_0258 0.68 -0.8 264,172 - B158DRAFT_0258 0.68 -1.0 264,172 - B158DRAFT_0258 0.68 -0.4 264,188 - B158DRAFT_0258 0.70 +0.1 264,200 + B158DRAFT_0258 0.71 -1.1 264,284 - B158DRAFT_0258 0.77 +0.8 264,284 - B158DRAFT_0258 0.77 -0.4 264,345 + B158DRAFT_0258 0.81 -1.3 264,430 - B158DRAFT_0258 0.88 +0.4 264,517 + +0.7 264,534 + +1.3 264,569 - +0.2 264,650 - +2.7 264,661 + -0.4 264,747 - B158DRAFT_0259 0.17 +0.5 264,804 + B158DRAFT_0259 0.25 -0.2 264,812 - B158DRAFT_0259 0.26 -1.0 264,838 - B158DRAFT_0259 0.29 +2.2 264,844 - B158DRAFT_0259 0.30 +2.2 264,852 - B158DRAFT_0259 0.31 +0.0 264,855 + B158DRAFT_0259 0.31 +2.5 264,863 - B158DRAFT_0259 0.32 +0.8 264,863 - B158DRAFT_0259 0.32 -1.1 264,894 - B158DRAFT_0259 0.36 -0.2 264,939 + B158DRAFT_0259 0.42 +0.4 264,947 - B158DRAFT_0259 0.43 +0.8 264,956 + B158DRAFT_0259 0.44 -0.7 264,998 + B158DRAFT_0259 0.49 +3.4 264,998 + B158DRAFT_0259 0.49 +0.2 264,998 + B158DRAFT_0259 0.49 +3.2 264,998 + B158DRAFT_0259 0.49 +1.8 264,998 + B158DRAFT_0259 0.49 +0.2 264,998 + B158DRAFT_0259 0.49 +0.0 264,998 + B158DRAFT_0259 0.49 +2.4 264,998 + B158DRAFT_0259 0.49 -0.6 265,006 - B158DRAFT_0259 0.50 +1.7 265,006 - B158DRAFT_0259 0.50 +1.8 265,006 - B158DRAFT_0259 0.50 +1.2 265,006 - B158DRAFT_0259 0.50 +3.7 265,017 + B158DRAFT_0259 0.51 +0.5 265,034 + B158DRAFT_0259 0.54 +0.6 265,056 + B158DRAFT_0259 0.56 +1.0 265,056 + B158DRAFT_0259 0.56 -0.2 265,064 - B158DRAFT_0259 0.57 +1.1 265,064 - B158DRAFT_0259 0.57 +0.9 265,064 - B158DRAFT_0259 0.57 +0.3 265,064 - B158DRAFT_0259 0.57 -1.0 265,080 - B158DRAFT_0259 0.59 +2.2 265,291 + B158DRAFT_0259 0.86 +0.9 265,299 - B158DRAFT_0259 0.87 +1.1 265,316 + B158DRAFT_0259 0.89 +1.4 265,350 + +2.7 265,351 - -0.8 265,358 - +2.2 265,493 + B158DRAFT_0260 0.14 +0.6 265,501 - B158DRAFT_0260 0.16 -1.2 265,521 + B158DRAFT_0260 0.20 -0.5 265,576 - B158DRAFT_0260 0.32 -0.7 265,623 - B158DRAFT_0260 0.42 -0.5 265,832 - B158DRAFT_0260 0.87 -1.0 265,839 + B158DRAFT_0260 0.89 +1.3 266,044 + +0.9 266,129 + +0.9 266,129 + +1.2 266,129 + -0.2 266,129 + -2.6 266,129 + +0.3 266,129 + -0.1 266,129 + -0.4 266,129 + +0.1 266,129 + -0.2 266,129 + -1.3 266,136 + -1.8 266,137 - -0.5 266,137 - -0.7 266,137 - +0.4 266,137 - +0.7 266,137 - -0.6 266,137 - -2.2 266,137 - -1.0 266,137 - -0.3 266,137 - -1.0 266,137 - -0.9 266,137 - +1.9 266,144 - -1.3 266,213 + B158DRAFT_0261 0.12 -3.1 266,221 - B158DRAFT_0261 0.12 +0.2 266,228 - B158DRAFT_0261 0.12 -0.9 266,228 - B158DRAFT_0261 0.12 -1.3 266,228 - B158DRAFT_0261 0.12 -1.2 266,248 + B158DRAFT_0261 0.13 -1.1 266,256 - B158DRAFT_0261 0.14 -0.3 266,264 + B158DRAFT_0261 0.14 -1.1 266,264 + B158DRAFT_0261 0.14 +0.8 266,269 + B158DRAFT_0261 0.14 -1.5 266,320 + B158DRAFT_0261 0.17 -0.4 266,320 + B158DRAFT_0261 0.17 -1.5 266,320 + B158DRAFT_0261 0.17 -1.1 266,320 + B158DRAFT_0261 0.17 +0.1 266,320 + B158DRAFT_0261 0.17 -1.4 266,320 + B158DRAFT_0261 0.17 +0.2 266,320 + B158DRAFT_0261 0.17 +0.5 266,320 + B158DRAFT_0261 0.17 -0.5 266,328 + B158DRAFT_0261 0.17 -0.3 266,328 - B158DRAFT_0261 0.17 -1.0 266,328 - B158DRAFT_0261 0.17 -1.2 266,328 - B158DRAFT_0261 0.17 +2.0 266,328 - B158DRAFT_0261 0.17 -1.3 266,328 - B158DRAFT_0261 0.17 -1.5 266,351 - B158DRAFT_0261 0.18 -0.9 266,351 - B158DRAFT_0261 0.18 -1.3 266,351 - B158DRAFT_0261 0.18 +0.9
Or see this region's nucleotide sequence