Experiment: m.b. nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt B158DRAFT_1114 and B158DRAFT_1115 are separated by 6 nucleotides B158DRAFT_1115 and B158DRAFT_1116 are separated by 5 nucleotides B158DRAFT_1116 and B158DRAFT_1117 are separated by 47 nucleotides
B158DRAFT_1114: B158DRAFT_1114 - S-adenosylhomocysteine hydrolase, at 1,151,052 to 1,152,107
_1114
B158DRAFT_1115: B158DRAFT_1115 - Predicted esterase of the alpha-beta hydrolase superfamily, at 1,152,114 to 1,153,268
_1115
B158DRAFT_1116: B158DRAFT_1116 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 1,153,274 to 1,153,825
_1116
B158DRAFT_1117: B158DRAFT_1117 - prolyl-tRNA synthetase (EC 6.1.1.15), at 1,153,873 to 1,155,588
_1117
Position (kb)
1152
1153
1154 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1151.480 kb on + strand, within B158DRAFT_1114 at 1151.480 kb on + strand, within B158DRAFT_1114 at 1151.488 kb on - strand, within B158DRAFT_1114 at 1151.538 kb on + strand, within B158DRAFT_1114 at 1151.546 kb on - strand, within B158DRAFT_1114 at 1151.546 kb on - strand, within B158DRAFT_1114 at 1151.556 kb on - strand, within B158DRAFT_1114 at 1151.618 kb on + strand, within B158DRAFT_1114 at 1151.638 kb on - strand, within B158DRAFT_1114 at 1151.655 kb on - strand, within B158DRAFT_1114 at 1151.655 kb on - strand, within B158DRAFT_1114 at 1151.728 kb on - strand, within B158DRAFT_1114 at 1151.730 kb on + strand, within B158DRAFT_1114 at 1151.773 kb on + strand, within B158DRAFT_1114 at 1151.827 kb on + strand, within B158DRAFT_1114 at 1151.835 kb on - strand, within B158DRAFT_1114 at 1151.914 kb on - strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.928 kb on + strand, within B158DRAFT_1114 at 1151.949 kb on - strand, within B158DRAFT_1114 at 1152.009 kb on + strand at 1152.042 kb on + strand at 1152.059 kb on - strand at 1152.065 kb on + strand at 1152.070 kb on + strand at 1152.113 kb on + strand at 1152.170 kb on + strand at 1152.217 kb on - strand at 1152.254 kb on - strand, within B158DRAFT_1115 at 1152.341 kb on + strand, within B158DRAFT_1115 at 1152.341 kb on + strand, within B158DRAFT_1115 at 1152.341 kb on + strand, within B158DRAFT_1115 at 1152.341 kb on + strand, within B158DRAFT_1115 at 1152.375 kb on - strand, within B158DRAFT_1115 at 1152.375 kb on - strand, within B158DRAFT_1115 at 1152.377 kb on + strand, within B158DRAFT_1115 at 1152.403 kb on + strand, within B158DRAFT_1115 at 1152.431 kb on - strand, within B158DRAFT_1115 at 1152.473 kb on - strand, within B158DRAFT_1115 at 1152.558 kb on + strand, within B158DRAFT_1115 at 1152.566 kb on - strand, within B158DRAFT_1115 at 1152.568 kb on - strand, within B158DRAFT_1115 at 1152.607 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.609 kb on + strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.617 kb on - strand, within B158DRAFT_1115 at 1152.678 kb on - strand, within B158DRAFT_1115 at 1152.710 kb on - strand, within B158DRAFT_1115 at 1152.725 kb on - strand, within B158DRAFT_1115 at 1152.731 kb on - strand, within B158DRAFT_1115 at 1152.731 kb on - strand, within B158DRAFT_1115 at 1152.731 kb on - strand, within B158DRAFT_1115 at 1152.768 kb on + strand, within B158DRAFT_1115 at 1152.776 kb on - strand, within B158DRAFT_1115 at 1152.805 kb on + strand, within B158DRAFT_1115 at 1152.813 kb on - strand, within B158DRAFT_1115 at 1152.821 kb on + strand, within B158DRAFT_1115 at 1152.821 kb on - strand, within B158DRAFT_1115 at 1152.911 kb on + strand, within B158DRAFT_1115 at 1153.025 kb on + strand, within B158DRAFT_1115 at 1153.025 kb on + strand, within B158DRAFT_1115 at 1153.025 kb on + strand, within B158DRAFT_1115 at 1153.052 kb on + strand, within B158DRAFT_1115 at 1153.060 kb on - strand, within B158DRAFT_1115 at 1153.070 kb on - strand, within B158DRAFT_1115 at 1153.100 kb on + strand, within B158DRAFT_1115 at 1153.186 kb on + strand at 1153.231 kb on + strand at 1153.239 kb on - strand at 1153.247 kb on + strand at 1153.249 kb on + strand at 1153.262 kb on - strand at 1153.310 kb on - strand at 1153.310 kb on - strand at 1153.333 kb on + strand, within B158DRAFT_1116 at 1153.367 kb on - strand, within B158DRAFT_1116 at 1153.371 kb on - strand, within B158DRAFT_1116 at 1153.412 kb on + strand, within B158DRAFT_1116 at 1153.444 kb on - strand, within B158DRAFT_1116 at 1153.448 kb on + strand, within B158DRAFT_1116 at 1153.456 kb on - strand, within B158DRAFT_1116 at 1153.482 kb on + strand, within B158DRAFT_1116 at 1153.482 kb on + strand, within B158DRAFT_1116 at 1153.482 kb on + strand, within B158DRAFT_1116 at 1153.482 kb on + strand, within B158DRAFT_1116 at 1153.490 kb on - strand, within B158DRAFT_1116 at 1153.506 kb on - strand, within B158DRAFT_1116 at 1153.559 kb on + strand, within B158DRAFT_1116 at 1153.568 kb on - strand, within B158DRAFT_1116 at 1153.641 kb on + strand, within B158DRAFT_1116 at 1153.649 kb on - strand, within B158DRAFT_1116 at 1153.649 kb on - strand, within B158DRAFT_1116 at 1153.660 kb on + strand, within B158DRAFT_1116 at 1153.691 kb on + strand, within B158DRAFT_1116 at 1153.783 kb on - strand at 1153.800 kb on + strand at 1153.829 kb on + strand at 1153.829 kb on + strand at 1153.836 kb on + strand at 1153.837 kb on - strand at 1153.844 kb on - strand at 1153.846 kb on + strand at 1153.846 kb on + strand at 1153.854 kb on - strand at 1153.856 kb on + strand at 1153.864 kb on - strand at 1153.888 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 20 mM remove 1,151,480 + B158DRAFT_1114 0.41 -0.4 1,151,480 + B158DRAFT_1114 0.41 -1.5 1,151,488 - B158DRAFT_1114 0.41 -1.9 1,151,538 + B158DRAFT_1114 0.46 +1.9 1,151,546 - B158DRAFT_1114 0.47 -2.7 1,151,546 - B158DRAFT_1114 0.47 +1.0 1,151,556 - B158DRAFT_1114 0.48 -2.8 1,151,618 + B158DRAFT_1114 0.54 -0.3 1,151,638 - B158DRAFT_1114 0.55 -0.1 1,151,655 - B158DRAFT_1114 0.57 -0.4 1,151,655 - B158DRAFT_1114 0.57 +0.3 1,151,728 - B158DRAFT_1114 0.64 -0.3 1,151,730 + B158DRAFT_1114 0.64 -0.2 1,151,773 + B158DRAFT_1114 0.68 +0.8 1,151,827 + B158DRAFT_1114 0.73 +2.0 1,151,835 - B158DRAFT_1114 0.74 -0.9 1,151,914 - B158DRAFT_1114 0.82 +0.1 1,151,928 + B158DRAFT_1114 0.83 -0.6 1,151,928 + B158DRAFT_1114 0.83 +0.1 1,151,928 + B158DRAFT_1114 0.83 -2.1 1,151,928 + B158DRAFT_1114 0.83 +1.1 1,151,928 + B158DRAFT_1114 0.83 -0.7 1,151,949 - B158DRAFT_1114 0.85 +0.1 1,152,009 + +1.2 1,152,042 + -0.4 1,152,059 - +0.6 1,152,065 + -3.0 1,152,070 + -0.4 1,152,113 + -0.4 1,152,170 + -0.3 1,152,217 - +0.2 1,152,254 - B158DRAFT_1115 0.12 -1.2 1,152,341 + B158DRAFT_1115 0.20 -0.5 1,152,341 + B158DRAFT_1115 0.20 -0.9 1,152,341 + B158DRAFT_1115 0.20 -1.2 1,152,341 + B158DRAFT_1115 0.20 -0.1 1,152,375 - B158DRAFT_1115 0.23 -0.9 1,152,375 - B158DRAFT_1115 0.23 +0.4 1,152,377 + B158DRAFT_1115 0.23 -0.4 1,152,403 + B158DRAFT_1115 0.25 -0.5 1,152,431 - B158DRAFT_1115 0.27 -1.1 1,152,473 - B158DRAFT_1115 0.31 +0.5 1,152,558 + B158DRAFT_1115 0.38 -0.7 1,152,566 - B158DRAFT_1115 0.39 -0.1 1,152,568 - B158DRAFT_1115 0.39 -2.1 1,152,607 + B158DRAFT_1115 0.43 -0.7 1,152,609 + B158DRAFT_1115 0.43 -0.4 1,152,609 + B158DRAFT_1115 0.43 +0.5 1,152,609 + B158DRAFT_1115 0.43 +2.0 1,152,609 + B158DRAFT_1115 0.43 -0.3 1,152,609 + B158DRAFT_1115 0.43 -0.9 1,152,609 + B158DRAFT_1115 0.43 +2.1 1,152,617 - B158DRAFT_1115 0.44 -1.7 1,152,617 - B158DRAFT_1115 0.44 -0.6 1,152,617 - B158DRAFT_1115 0.44 +1.4 1,152,617 - B158DRAFT_1115 0.44 -0.4 1,152,617 - B158DRAFT_1115 0.44 +0.0 1,152,678 - B158DRAFT_1115 0.49 -0.6 1,152,710 - B158DRAFT_1115 0.52 -1.7 1,152,725 - B158DRAFT_1115 0.53 +0.7 1,152,731 - B158DRAFT_1115 0.53 +0.5 1,152,731 - B158DRAFT_1115 0.53 -0.6 1,152,731 - B158DRAFT_1115 0.53 -2.4 1,152,768 + B158DRAFT_1115 0.57 -1.7 1,152,776 - B158DRAFT_1115 0.57 -0.1 1,152,805 + B158DRAFT_1115 0.60 +1.5 1,152,813 - B158DRAFT_1115 0.61 -0.7 1,152,821 + B158DRAFT_1115 0.61 -1.7 1,152,821 - B158DRAFT_1115 0.61 -1.7 1,152,911 + B158DRAFT_1115 0.69 +0.7 1,153,025 + B158DRAFT_1115 0.79 -0.9 1,153,025 + B158DRAFT_1115 0.79 +0.8 1,153,025 + B158DRAFT_1115 0.79 -0.2 1,153,052 + B158DRAFT_1115 0.81 +0.1 1,153,060 - B158DRAFT_1115 0.82 +0.4 1,153,070 - B158DRAFT_1115 0.83 -0.6 1,153,100 + B158DRAFT_1115 0.85 +1.4 1,153,186 + -0.8 1,153,231 + -0.0 1,153,239 - +0.5 1,153,247 + +0.3 1,153,249 + -0.1 1,153,262 - +0.5 1,153,310 - +0.4 1,153,310 - +1.7 1,153,333 + B158DRAFT_1116 0.11 +2.4 1,153,367 - B158DRAFT_1116 0.17 -1.5 1,153,371 - B158DRAFT_1116 0.18 +1.4 1,153,412 + B158DRAFT_1116 0.25 -0.8 1,153,444 - B158DRAFT_1116 0.31 -0.2 1,153,448 + B158DRAFT_1116 0.32 +0.3 1,153,456 - B158DRAFT_1116 0.33 -1.1 1,153,482 + B158DRAFT_1116 0.38 -0.2 1,153,482 + B158DRAFT_1116 0.38 +0.5 1,153,482 + B158DRAFT_1116 0.38 -1.5 1,153,482 + B158DRAFT_1116 0.38 +0.7 1,153,490 - B158DRAFT_1116 0.39 +1.8 1,153,506 - B158DRAFT_1116 0.42 -1.0 1,153,559 + B158DRAFT_1116 0.52 +0.8 1,153,568 - B158DRAFT_1116 0.53 -0.8 1,153,641 + B158DRAFT_1116 0.66 -2.1 1,153,649 - B158DRAFT_1116 0.68 -0.1 1,153,649 - B158DRAFT_1116 0.68 +1.4 1,153,660 + B158DRAFT_1116 0.70 +0.5 1,153,691 + B158DRAFT_1116 0.76 +0.7 1,153,783 - -1.8 1,153,800 + +0.4 1,153,829 + +0.4 1,153,829 + -0.1 1,153,836 + +1.3 1,153,837 - -2.5 1,153,844 - +1.0 1,153,846 + -0.8 1,153,846 + +0.3 1,153,854 - -2.0 1,153,856 + -0.7 1,153,864 - -0.4 1,153,888 + +1.4
Or see this region's nucleotide sequence