Experiment: Plant=Sorghum_Btx_430; PlantTreatment=None; Sample=leaf; GrowthSubstrate=agar; Collection=Direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0148 and Atu0149 are separated by 184 nucleotides Atu0149 and Atu0150 are separated by 6 nucleotides Atu0150 and Atu0151 are separated by 75 nucleotides Atu0151 and Atu0152 are separated by 70 nucleotides Atu0152 and Atu0153 overlap by 11 nucleotides
Atu0148: Atu0148 - Atu0148-1 mutant of a conserved hypothetical protein, at 156,680 to 157,276
Atu0148
Atu0149: Atu0149 - enoyl-(acyl-carrier-protein) reductase, at 157,461 to 158,267
Atu0149
Atu0150: Atu0150 - 3-oxoacyl-(acyl-carrier-protein) synthase, at 158,274 to 159,497
Atu0150
Atu0151: Atu0151 - D-3-hydroxydecanoyl-(acyl carrier-protein) dehydratase, at 159,573 to 160,088
Atu0151
Atu0152: Atu0152 - hypothetical protein, at 160,159 to 160,416
Atu0152
Atu0153: Atu0153 - transcriptional regulator, Fur family, at 160,406 to 160,825
Atu0153
Position (kb) 158
159
160 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 157.291 kb on + strand at 157.482 kb on - strand at 157.482 kb on - strand at 157.482 kb on - strand at 157.561 kb on + strand, within Atu0149 at 157.588 kb on - strand, within Atu0149 at 157.795 kb on + strand, within Atu0149 at 157.828 kb on + strand, within Atu0149 at 157.868 kb on + strand, within Atu0149 at 158.091 kb on + strand, within Atu0149 at 158.160 kb on - strand, within Atu0149 at 158.257 kb on + strand at 160.217 kb on + strand, within Atu0152 at 160.329 kb on - strand, within Atu0152 at 160.429 kb on - strand at 160.442 kb on - strand at 160.447 kb on - strand at 160.457 kb on - strand, within Atu0153 at 160.481 kb on + strand, within Atu0153 at 160.481 kb on + strand, within Atu0153
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=Sorghum_Btx_430; PlantTreatment=None; Sample=leaf; GrowthSubstrate=agar; Collection=Direct remove 157,291 + +0.1 157,482 - +1.8 157,482 - +1.3 157,482 - +0.6 157,561 + Atu0149 0.12 -0.2 157,588 - Atu0149 0.16 -1.2 157,795 + Atu0149 0.41 +0.2 157,828 + Atu0149 0.45 -0.4 157,868 + Atu0149 0.50 -1.4 158,091 + Atu0149 0.78 +2.5 158,160 - Atu0149 0.87 -1.2 158,257 + +0.6 160,217 + Atu0152 0.22 +0.1 160,329 - Atu0152 0.66 +2.1 160,429 - -2.1 160,442 - -0.4 160,447 - -2.4 160,457 - Atu0153 0.12 -3.3 160,481 + Atu0153 0.18 -4.3 160,481 + Atu0153 0.18 -0.9
Or see this region's nucleotide sequence