Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0186 and PP_0187 overlap by 4 nucleotides PP_0187 and PP_0188 are separated by 18 nucleotides PP_0188 and PP_0189 overlap by 4 nucleotides
PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923
_0186
PP_0187: PP_0187 - Uroporphyrinogen-III synthase, at 238,920 to 239,687
_0187
PP_0188: PP_0188 - putative Uroporphyrin-III C-methyltransferase, at 239,706 to 240,833
_0188
PP_0189: PP_0189 - putative heme biosynthesis protein, at 240,830 to 242,068
_0189
Position (kb) 239
240
241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.686 kb on - strand at 239.693 kb on + strand at 239.694 kb on - strand at 239.694 kb on - strand at 239.730 kb on + strand at 239.730 kb on + strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.762 kb on + strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.872 kb on + strand, within PP_0188 at 239.872 kb on + strand, within PP_0188 at 239.873 kb on - strand, within PP_0188 at 240.028 kb on + strand, within PP_0188 at 240.161 kb on + strand, within PP_0188 at 240.323 kb on + strand, within PP_0188 at 240.324 kb on - strand, within PP_0188 at 240.365 kb on - strand, within PP_0188 at 240.402 kb on - strand, within PP_0188 at 240.475 kb on + strand, within PP_0188 at 240.483 kb on + strand, within PP_0188 at 240.484 kb on - strand, within PP_0188 at 240.575 kb on + strand, within PP_0188 at 240.576 kb on - strand, within PP_0188 at 240.652 kb on + strand, within PP_0188 at 240.653 kb on - strand, within PP_0188 at 240.755 kb on + strand at 240.756 kb on - strand at 240.783 kb on + strand at 240.783 kb on + strand at 240.783 kb on + strand at 240.784 kb on - strand at 240.784 kb on - strand at 240.785 kb on + strand at 240.786 kb on - strand at 240.786 kb on - strand at 240.786 kb on - strand at 240.842 kb on + strand at 240.842 kb on + strand at 240.843 kb on - strand at 240.911 kb on + strand at 240.911 kb on + strand at 240.911 kb on + strand at 240.912 kb on - strand at 240.912 kb on - strand at 240.912 kb on - strand at 240.915 kb on + strand at 240.916 kb on - strand at 240.926 kb on + strand at 240.927 kb on - strand at 241.082 kb on - strand, within PP_0189 at 241.190 kb on + strand, within PP_0189 at 241.191 kb on - strand, within PP_0189 at 241.191 kb on - strand, within PP_0189 at 241.191 kb on - strand, within PP_0189 at 241.193 kb on + strand, within PP_0189 at 241.193 kb on + strand, within PP_0189 at 241.194 kb on - strand, within PP_0189 at 241.194 kb on - strand, within PP_0189 at 241.421 kb on + strand, within PP_0189 at 241.421 kb on + strand, within PP_0189 at 241.421 kb on + strand, within PP_0189 at 241.422 kb on - strand, within PP_0189 at 241.422 kb on - strand, within PP_0189 at 241.563 kb on + strand, within PP_0189 at 241.653 kb on - strand, within PP_0189 at 241.674 kb on + strand, within PP_0189 at 241.675 kb on - strand, within PP_0189 at 241.728 kb on - strand, within PP_0189
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 239,685 + +0.6 239,685 + -2.8 239,685 + -2.1 239,685 + -3.2 239,685 + -0.6 239,686 - +0.6 239,693 + -1.8 239,694 - -2.0 239,694 - -2.3 239,730 + +0.1 239,730 + +1.5 239,731 - -3.3 239,731 - +0.4 239,731 - -0.8 239,762 + -2.1 239,763 - +1.8 239,763 - -2.8 239,763 - -1.5 239,872 + PP_0188 0.15 -1.7 239,872 + PP_0188 0.15 +0.7 239,873 - PP_0188 0.15 -0.6 240,028 + PP_0188 0.29 -2.9 240,161 + PP_0188 0.40 -0.8 240,323 + PP_0188 0.55 -3.2 240,324 - PP_0188 0.55 -3.2 240,365 - PP_0188 0.58 +1.1 240,402 - PP_0188 0.62 -2.0 240,475 + PP_0188 0.68 -3.6 240,483 + PP_0188 0.69 -0.2 240,484 - PP_0188 0.69 +1.2 240,575 + PP_0188 0.77 -2.7 240,576 - PP_0188 0.77 +0.5 240,652 + PP_0188 0.84 -3.0 240,653 - PP_0188 0.84 +0.3 240,755 + +0.5 240,756 - -0.6 240,783 + -1.3 240,783 + -0.1 240,783 + -3.6 240,784 - +0.3 240,784 - +1.4 240,785 + -2.4 240,786 - -2.4 240,786 - -2.4 240,786 - -2.3 240,842 + -3.4 240,842 + -3.7 240,843 - +0.7 240,911 + -2.9 240,911 + +2.1 240,911 + -2.6 240,912 - -2.7 240,912 - -3.3 240,912 - -3.3 240,915 + +1.7 240,916 - -2.2 240,926 + -2.0 240,927 - +1.7 241,082 - PP_0189 0.20 +1.1 241,190 + PP_0189 0.29 -2.3 241,191 - PP_0189 0.29 -0.6 241,191 - PP_0189 0.29 +0.3 241,191 - PP_0189 0.29 +0.6 241,193 + PP_0189 0.29 +0.9 241,193 + PP_0189 0.29 +0.5 241,194 - PP_0189 0.29 +0.9 241,194 - PP_0189 0.29 -0.1 241,421 + PP_0189 0.48 +0.9 241,421 + PP_0189 0.48 -1.6 241,421 + PP_0189 0.48 +0.7 241,422 - PP_0189 0.48 -0.0 241,422 - PP_0189 0.48 +0.1 241,563 + PP_0189 0.59 -0.1 241,653 - PP_0189 0.66 +1.5 241,674 + PP_0189 0.68 -2.8 241,675 - PP_0189 0.68 -2.6 241,728 - PP_0189 0.72 +0.2
Or see this region's nucleotide sequence