Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5665 and PP_5666 are separated by 58 nucleotides PP_5666 and PP_5667 overlap by 1 nucleotides PP_5667 and PP_4240 are separated by 4 nucleotides
PP_5665: PP_5665 - putative microcin B17-like protein, McbA family, at 4,812,473 to 4,812,715
_5665
PP_5666: PP_5666 - putative microcin B17-like processing protein, McbB family, at 4,812,774 to 4,813,637
_5666
PP_5667: PP_5667 - putative microcin B17-like-processing protein, McbC family, at 4,813,637 to 4,814,428
_5667
PP_4240: PP_4240 - putative Microcin b17 processing protein mcbd, at 4,814,433 to 4,815,635
_4240
Position (kb) 4813
4814
4815 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4812.692 kb on - strand at 4813.050 kb on + strand, within PP_5666 at 4813.149 kb on - strand, within PP_5666 at 4813.168 kb on + strand, within PP_5666 at 4813.229 kb on - strand, within PP_5666 at 4813.353 kb on - strand, within PP_5666 at 4813.475 kb on - strand, within PP_5666 at 4813.632 kb on - strand at 4813.721 kb on + strand, within PP_5667 at 4813.721 kb on + strand, within PP_5667 at 4813.962 kb on + strand, within PP_5667 at 4814.203 kb on + strand, within PP_5667 at 4814.207 kb on + strand, within PP_5667 at 4814.241 kb on - strand at 4814.288 kb on + strand, within PP_5667 at 4814.349 kb on - strand at 4814.350 kb on + strand at 4814.486 kb on + strand at 4814.547 kb on + strand at 4814.547 kb on + strand at 4814.656 kb on + strand, within PP_4240 at 4814.772 kb on + strand, within PP_4240 at 4814.864 kb on + strand, within PP_4240 at 4814.897 kb on + strand, within PP_4240 at 4815.084 kb on - strand, within PP_4240 at 4815.125 kb on - strand, within PP_4240 at 4815.130 kb on - strand, within PP_4240 at 4815.130 kb on - strand, within PP_4240 at 4815.181 kb on - strand, within PP_4240
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 4,812,692 - +0.2 4,813,050 + PP_5666 0.32 -2.2 4,813,149 - PP_5666 0.43 -0.1 4,813,168 + PP_5666 0.46 +0.6 4,813,229 - PP_5666 0.53 -0.7 4,813,353 - PP_5666 0.67 +0.6 4,813,475 - PP_5666 0.81 +1.8 4,813,632 - -0.5 4,813,721 + PP_5667 0.11 -3.0 4,813,721 + PP_5667 0.11 +1.1 4,813,962 + PP_5667 0.41 +0.2 4,814,203 + PP_5667 0.71 +1.9 4,814,207 + PP_5667 0.72 -0.5 4,814,241 - +1.8 4,814,288 + PP_5667 0.82 +1.5 4,814,349 - +0.3 4,814,350 + -1.7 4,814,486 + +3.0 4,814,547 + -1.6 4,814,547 + -1.0 4,814,656 + PP_4240 0.19 -0.1 4,814,772 + PP_4240 0.28 +0.8 4,814,864 + PP_4240 0.36 +1.2 4,814,897 + PP_4240 0.39 +1.0 4,815,084 - PP_4240 0.54 +1.8 4,815,125 - PP_4240 0.58 -1.3 4,815,130 - PP_4240 0.58 +0.4 4,815,130 - PP_4240 0.58 +1.2 4,815,181 - PP_4240 0.62 -0.2
Or see this region's nucleotide sequence