Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3109 and PP_5579 are separated by 58 nucleotides PP_5579 and PP_5580 are separated by 300 nucleotides PP_5580 and PP_5582 are separated by 879 nucleotides
PP_3109: PP_3109 - conserved protein of unknown function, at 3,517,884 to 3,518,315
_3109
PP_5579: PP_5579 - conserved protein of unknown function, at 3,518,374 to 3,518,853
_5579
PP_5580: PP_5580 - protein of unknown function, at 3,519,154 to 3,519,381
_5580
PP_5582: PP_5582 - conserved protein of unknown function, at 3,520,261 to 3,520,728
_5582
Position (kb)
3519
3520 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3518.280 kb on - strand at 3518.463 kb on + strand, within PP_5579 at 3518.469 kb on + strand, within PP_5579 at 3518.616 kb on + strand, within PP_5579 at 3518.671 kb on - strand, within PP_5579 at 3518.740 kb on + strand, within PP_5579 at 3518.741 kb on - strand, within PP_5579 at 3518.758 kb on + strand, within PP_5579 at 3518.795 kb on + strand, within PP_5579 at 3518.797 kb on + strand, within PP_5579 at 3518.798 kb on - strand, within PP_5579 at 3518.851 kb on + strand at 3518.990 kb on - strand at 3519.009 kb on - strand at 3519.107 kb on + strand at 3519.119 kb on - strand at 3519.119 kb on - strand at 3519.146 kb on - strand at 3519.206 kb on + strand, within PP_5580 at 3519.323 kb on + strand, within PP_5580 at 3519.380 kb on - strand at 3519.457 kb on - strand at 3519.647 kb on - strand at 3519.690 kb on + strand at 3519.733 kb on + strand at 3519.733 kb on + strand at 3519.885 kb on + strand at 3519.924 kb on - strand at 3519.924 kb on - strand at 3519.956 kb on - strand at 3519.956 kb on - strand at 3519.958 kb on + strand at 3519.959 kb on - strand at 3519.976 kb on - strand at 3519.976 kb on - strand at 3519.999 kb on + strand at 3520.028 kb on + strand at 3520.037 kb on + strand at 3520.045 kb on + strand at 3520.083 kb on - strand at 3520.094 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 3,518,280 - -0.5 3,518,463 + PP_5579 0.19 +1.7 3,518,469 + PP_5579 0.20 -0.3 3,518,616 + PP_5579 0.50 +1.0 3,518,671 - PP_5579 0.62 -0.7 3,518,740 + PP_5579 0.76 -1.2 3,518,741 - PP_5579 0.76 +1.0 3,518,758 + PP_5579 0.80 -0.6 3,518,795 + PP_5579 0.88 +1.4 3,518,797 + PP_5579 0.88 +1.9 3,518,798 - PP_5579 0.88 +0.3 3,518,851 + -0.4 3,518,990 - +0.9 3,519,009 - -0.3 3,519,107 + -0.5 3,519,119 - +1.5 3,519,119 - +0.6 3,519,146 - +1.3 3,519,206 + PP_5580 0.23 +0.9 3,519,323 + PP_5580 0.74 +1.4 3,519,380 - -1.2 3,519,457 - -2.4 3,519,647 - -0.0 3,519,690 + +1.9 3,519,733 + -0.3 3,519,733 + +1.0 3,519,885 + -0.0 3,519,924 - -3.0 3,519,924 - -0.7 3,519,956 - -4.2 3,519,956 - +2.2 3,519,958 + +1.3 3,519,959 - +1.6 3,519,976 - -0.4 3,519,976 - +0.5 3,519,999 + -3.5 3,520,028 + -4.3 3,520,037 + +0.9 3,520,045 + +0.2 3,520,083 - +1.3 3,520,094 - -2.0
Or see this region's nucleotide sequence