Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4330 and PP_4331 are separated by 107 nucleotides PP_4331 and PP_4332 are separated by 1 nucleotides PP_4332 and PP_4333 are separated by 41 nucleotides PP_4333 and PP_4334 overlap by 1 nucleotides
PP_4330: PP_4330 - putative toxin-related protein, at 4,919,592 to 4,922,273
_4330
PP_4331: PP_4331 - conserved protein of unknown function, at 4,922,381 to 4,922,776
_4331
PP_4332: PP_4332 - Chemotaxis protein CheW, at 4,922,778 to 4,923,257
_4332
PP_4333: PP_4333 - CheW domain protein, at 4,923,299 to 4,924,195
_4333
PP_4334: PP_4334 - ParA family protein, at 4,924,195 to 4,925,049
_4334
Position (kb) 4922
4923
4924 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4921.995 kb on + strand, within PP_4330 at 4921.995 kb on + strand, within PP_4330 at 4921.996 kb on - strand, within PP_4330 at 4922.043 kb on + strand at 4922.062 kb on - strand at 4922.108 kb on - strand at 4922.169 kb on + strand at 4922.169 kb on + strand at 4922.170 kb on - strand at 4922.198 kb on + strand at 4922.199 kb on - strand at 4922.199 kb on - strand at 4922.287 kb on + strand at 4922.288 kb on - strand at 4922.458 kb on + strand, within PP_4331 at 4922.610 kb on + strand, within PP_4331 at 4922.611 kb on - strand, within PP_4331 at 4922.706 kb on + strand, within PP_4331 at 4922.706 kb on + strand, within PP_4331 at 4922.706 kb on + strand, within PP_4331 at 4922.707 kb on - strand, within PP_4331 at 4922.707 kb on - strand, within PP_4331 at 4922.707 kb on - strand, within PP_4331 at 4922.707 kb on - strand, within PP_4331 at 4922.707 kb on - strand, within PP_4331 at 4922.768 kb on + strand at 4922.769 kb on - strand at 4922.877 kb on + strand, within PP_4332 at 4923.106 kb on + strand, within PP_4332 at 4923.106 kb on + strand, within PP_4332 at 4923.107 kb on - strand, within PP_4332 at 4923.206 kb on + strand, within PP_4332 at 4923.206 kb on + strand, within PP_4332 at 4923.263 kb on + strand at 4923.502 kb on - strand, within PP_4333 at 4923.502 kb on - strand, within PP_4333 at 4923.502 kb on - strand, within PP_4333 at 4923.522 kb on + strand, within PP_4333 at 4923.522 kb on + strand, within PP_4333 at 4923.522 kb on + strand, within PP_4333 at 4923.523 kb on - strand, within PP_4333 at 4923.678 kb on + strand, within PP_4333 at 4923.678 kb on + strand, within PP_4333 at 4923.678 kb on + strand, within PP_4333 at 4923.678 kb on + strand, within PP_4333 at 4923.678 kb on + strand, within PP_4333 at 4923.679 kb on - strand, within PP_4333 at 4923.794 kb on + strand, within PP_4333 at 4923.950 kb on + strand, within PP_4333 at 4924.144 kb on - strand at 4924.221 kb on - strand at 4924.221 kb on - strand at 4924.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 4,921,995 + PP_4330 0.90 -3.4 4,921,995 + PP_4330 0.90 +1.4 4,921,996 - PP_4330 0.90 +0.2 4,922,043 + -0.2 4,922,062 - -3.6 4,922,108 - -1.1 4,922,169 + +0.1 4,922,169 + +1.0 4,922,170 - -0.7 4,922,198 + +0.9 4,922,199 - +0.7 4,922,199 - -3.4 4,922,287 + -0.3 4,922,288 - -3.2 4,922,458 + PP_4331 0.19 -2.5 4,922,610 + PP_4331 0.58 -0.6 4,922,611 - PP_4331 0.58 +0.5 4,922,706 + PP_4331 0.82 +0.2 4,922,706 + PP_4331 0.82 -1.6 4,922,706 + PP_4331 0.82 +0.6 4,922,707 - PP_4331 0.82 -0.2 4,922,707 - PP_4331 0.82 -0.4 4,922,707 - PP_4331 0.82 -1.0 4,922,707 - PP_4331 0.82 -0.4 4,922,707 - PP_4331 0.82 -1.0 4,922,768 + +0.4 4,922,769 - -3.7 4,922,877 + PP_4332 0.21 +1.7 4,923,106 + PP_4332 0.68 -2.6 4,923,106 + PP_4332 0.68 +1.8 4,923,107 - PP_4332 0.69 +0.1 4,923,206 + PP_4332 0.89 +0.8 4,923,206 + PP_4332 0.89 +0.4 4,923,263 + +1.2 4,923,502 - PP_4333 0.23 -3.6 4,923,502 - PP_4333 0.23 -2.9 4,923,502 - PP_4333 0.23 -0.4 4,923,522 + PP_4333 0.25 +0.0 4,923,522 + PP_4333 0.25 +1.5 4,923,522 + PP_4333 0.25 -1.3 4,923,523 - PP_4333 0.25 -0.5 4,923,678 + PP_4333 0.42 +0.6 4,923,678 + PP_4333 0.42 -0.4 4,923,678 + PP_4333 0.42 -0.7 4,923,678 + PP_4333 0.42 +0.2 4,923,678 + PP_4333 0.42 -0.4 4,923,679 - PP_4333 0.42 +0.6 4,923,794 + PP_4333 0.55 -1.1 4,923,950 + PP_4333 0.73 -2.7 4,924,144 - -1.2 4,924,221 - +1.0 4,924,221 - +0.3 4,924,251 - +1.1
Or see this region's nucleotide sequence