Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2906 and PP_2907 overlap by 23 nucleotides PP_2907 and PP_2908 are separated by 74 nucleotides PP_2908 and PP_2909 are separated by 195 nucleotides
PP_2906: PP_2906 - Sensor histidine kinase, at 3,304,779 to 3,306,170
_2906
PP_2907: PP_2907 - DNA-binding response regulator, at 3,306,148 to 3,306,828
_2907
PP_2908: PP_2908 - DNA-binding transcriptional repressor, at 3,306,903 to 3,307,598
_2908
PP_2909: PP_2909 - glutarate hydroxylase <small>(from data)</small>, at 3,307,794 to 3,308,771
_2909
Position (kb) 3306
3307 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 3305.212 kb on - strand, within PP_2906 at 3305.601 kb on + strand, within PP_2906 at 3305.603 kb on + strand, within PP_2906 at 3305.604 kb on - strand, within PP_2906 at 3305.604 kb on - strand, within PP_2906 at 3305.696 kb on + strand, within PP_2906 at 3305.696 kb on + strand, within PP_2906 at 3305.697 kb on - strand, within PP_2906 at 3305.757 kb on + strand, within PP_2906 at 3305.757 kb on + strand, within PP_2906 at 3305.757 kb on + strand, within PP_2906 at 3306.044 kb on - strand at 3306.062 kb on - strand at 3306.132 kb on - strand at 3306.132 kb on - strand at 3306.149 kb on + strand at 3306.190 kb on - strand at 3306.360 kb on - strand, within PP_2907 at 3306.360 kb on - strand, within PP_2907 at 3306.463 kb on + strand, within PP_2907 at 3306.484 kb on + strand, within PP_2907 at 3306.484 kb on + strand, within PP_2907 at 3306.609 kb on + strand, within PP_2907 at 3306.846 kb on + strand at 3306.846 kb on + strand at 3306.846 kb on + strand at 3306.847 kb on - strand at 3306.904 kb on + strand at 3307.123 kb on + strand, within PP_2908 at 3307.124 kb on - strand at 3307.231 kb on - strand, within PP_2908 at 3307.231 kb on - strand, within PP_2908 at 3307.231 kb on - strand, within PP_2908 at 3307.253 kb on + strand, within PP_2908 at 3307.254 kb on - strand, within PP_2908 at 3307.254 kb on - strand, within PP_2908 at 3307.254 kb on - strand, within PP_2908 at 3307.348 kb on + strand, within PP_2908 at 3307.349 kb on - strand, within PP_2908 at 3307.474 kb on + strand, within PP_2908 at 3307.631 kb on - strand at 3307.673 kb on + strand at 3307.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 3,305,212 - PP_2906 0.31 +0.0 3,305,601 + PP_2906 0.59 +0.7 3,305,603 + PP_2906 0.59 +0.1 3,305,604 - PP_2906 0.59 +0.2 3,305,604 - PP_2906 0.59 -0.7 3,305,696 + PP_2906 0.66 -2.2 3,305,696 + PP_2906 0.66 +0.9 3,305,697 - PP_2906 0.66 -4.1 3,305,757 + PP_2906 0.70 -2.3 3,305,757 + PP_2906 0.70 -0.8 3,305,757 + PP_2906 0.70 +1.5 3,306,044 - +1.1 3,306,062 - -0.4 3,306,132 - -2.4 3,306,132 - +1.2 3,306,149 + -1.9 3,306,190 - -0.0 3,306,360 - PP_2907 0.31 +0.3 3,306,360 - PP_2907 0.31 -2.4 3,306,463 + PP_2907 0.46 +0.6 3,306,484 + PP_2907 0.49 -1.3 3,306,484 + PP_2907 0.49 +2.4 3,306,609 + PP_2907 0.68 +0.9 3,306,846 + +0.0 3,306,846 + +0.6 3,306,846 + +1.1 3,306,847 - +0.7 3,306,904 + +0.3 3,307,123 + PP_2908 0.32 -0.1 3,307,124 - +0.3 3,307,231 - PP_2908 0.47 -4.1 3,307,231 - PP_2908 0.47 +0.4 3,307,231 - PP_2908 0.47 -2.6 3,307,253 + PP_2908 0.50 -0.1 3,307,254 - PP_2908 0.50 -0.2 3,307,254 - PP_2908 0.50 -0.9 3,307,254 - PP_2908 0.50 +0.4 3,307,348 + PP_2908 0.64 -1.5 3,307,349 - PP_2908 0.64 -2.3 3,307,474 + PP_2908 0.82 -3.1 3,307,631 - -3.1 3,307,673 + -1.9 3,307,673 + -0.2
Or see this region's nucleotide sequence