Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2862 and PP_2863 are separated by 28 nucleotides PP_2863 and PP_2864 are separated by 58 nucleotides PP_2864 and PP_2865 overlap by 20 nucleotides
PP_2862: PP_2862 - Undecaprenyl-diphosphatase, at 3,264,844 to 3,265,677
_2862
PP_2863: PP_2863 - conserved membrane protein of unknown function, at 3,265,706 to 3,266,083
_2863
PP_2864: PP_2864 - conserved protein of unknown function, at 3,266,142 to 3,266,513
_2864
PP_2865: PP_2865 - Transcriptional regulator, LysR family, at 3,266,494 to 3,267,396
_2865
Position (kb)
3266
3267 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3265.158 kb on + strand, within PP_2862 at 3265.158 kb on + strand, within PP_2862 at 3265.168 kb on + strand, within PP_2862 at 3265.169 kb on - strand, within PP_2862 at 3265.450 kb on + strand, within PP_2862 at 3265.450 kb on + strand, within PP_2862 at 3265.451 kb on - strand, within PP_2862 at 3265.451 kb on - strand, within PP_2862 at 3265.459 kb on + strand, within PP_2862 at 3265.465 kb on + strand, within PP_2862 at 3265.547 kb on + strand, within PP_2862 at 3265.579 kb on - strand, within PP_2862 at 3265.579 kb on - strand, within PP_2862 at 3265.579 kb on - strand at 3265.579 kb on - strand, within PP_2862 at 3265.582 kb on + strand, within PP_2862 at 3265.583 kb on - strand, within PP_2862 at 3265.850 kb on + strand, within PP_2863 at 3265.851 kb on - strand, within PP_2863 at 3265.876 kb on + strand, within PP_2863 at 3265.876 kb on + strand, within PP_2863 at 3265.877 kb on - strand, within PP_2863 at 3265.891 kb on + strand, within PP_2863 at 3265.892 kb on - strand, within PP_2863 at 3265.892 kb on - strand, within PP_2863 at 3266.067 kb on - strand at 3266.067 kb on - strand at 3266.095 kb on - strand at 3266.187 kb on + strand, within PP_2864 at 3266.188 kb on - strand, within PP_2864 at 3266.281 kb on + strand, within PP_2864 at 3266.428 kb on + strand, within PP_2864 at 3266.501 kb on + strand at 3266.501 kb on + strand at 3266.501 kb on + strand at 3266.501 kb on + strand at 3266.502 kb on - strand at 3266.502 kb on - strand at 3266.502 kb on - strand at 3266.601 kb on - strand, within PP_2865 at 3266.707 kb on + strand, within PP_2865 at 3266.719 kb on - strand, within PP_2865 at 3266.722 kb on + strand, within PP_2865 at 3266.722 kb on + strand, within PP_2865 at 3266.722 kb on + strand, within PP_2865 at 3266.723 kb on - strand, within PP_2865 at 3266.803 kb on + strand, within PP_2865 at 3266.803 kb on + strand, within PP_2865 at 3266.963 kb on - strand, within PP_2865 at 3267.019 kb on + strand, within PP_2865 at 3267.019 kb on + strand, within PP_2865 at 3267.020 kb on - strand, within PP_2865 at 3267.020 kb on - strand, within PP_2865 at 3267.020 kb on - strand, within PP_2865 at 3267.020 kb on - strand, within PP_2865 at 3267.020 kb on - strand, within PP_2865 at 3267.115 kb on + strand, within PP_2865 at 3267.115 kb on + strand, within PP_2865 at 3267.203 kb on + strand, within PP_2865 at 3267.203 kb on + strand, within PP_2865 at 3267.203 kb on + strand, within PP_2865 at 3267.204 kb on - strand, within PP_2865 at 3267.204 kb on - strand, within PP_2865 at 3267.204 kb on - strand, within PP_2865 at 3267.204 kb on - strand, within PP_2865 at 3267.385 kb on - strand at 3267.385 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 3,265,158 + PP_2862 0.38 +0.2 3,265,158 + PP_2862 0.38 -1.2 3,265,168 + PP_2862 0.39 -0.3 3,265,169 - PP_2862 0.39 +0.4 3,265,450 + PP_2862 0.73 -0.5 3,265,450 + PP_2862 0.73 +0.6 3,265,451 - PP_2862 0.73 +0.8 3,265,451 - PP_2862 0.73 -0.3 3,265,459 + PP_2862 0.74 -0.3 3,265,465 + PP_2862 0.74 -0.5 3,265,547 + PP_2862 0.84 -1.2 3,265,579 - PP_2862 0.88 +0.5 3,265,579 - PP_2862 0.88 -0.1 3,265,579 - -0.8 3,265,579 - PP_2862 0.88 +0.3 3,265,582 + PP_2862 0.88 +0.8 3,265,583 - PP_2862 0.89 +1.5 3,265,850 + PP_2863 0.38 -0.2 3,265,851 - PP_2863 0.38 +0.4 3,265,876 + PP_2863 0.45 +2.5 3,265,876 + PP_2863 0.45 -1.0 3,265,877 - PP_2863 0.45 -4.3 3,265,891 + PP_2863 0.49 -0.8 3,265,892 - PP_2863 0.49 -2.2 3,265,892 - PP_2863 0.49 -0.3 3,266,067 - +0.2 3,266,067 - +0.8 3,266,095 - +0.6 3,266,187 + PP_2864 0.12 +0.7 3,266,188 - PP_2864 0.12 +1.9 3,266,281 + PP_2864 0.37 +1.2 3,266,428 + PP_2864 0.77 +0.4 3,266,501 + +0.9 3,266,501 + +0.4 3,266,501 + -0.4 3,266,501 + -3.3 3,266,502 - -0.3 3,266,502 - -0.3 3,266,502 - +1.3 3,266,601 - PP_2865 0.12 +0.9 3,266,707 + PP_2865 0.24 -1.7 3,266,719 - PP_2865 0.25 +0.8 3,266,722 + PP_2865 0.25 +0.6 3,266,722 + PP_2865 0.25 -3.8 3,266,722 + PP_2865 0.25 +0.5 3,266,723 - PP_2865 0.25 -0.0 3,266,803 + PP_2865 0.34 +1.1 3,266,803 + PP_2865 0.34 +0.7 3,266,963 - PP_2865 0.52 -2.3 3,267,019 + PP_2865 0.58 -3.3 3,267,019 + PP_2865 0.58 -0.5 3,267,020 - PP_2865 0.58 +1.0 3,267,020 - PP_2865 0.58 +0.1 3,267,020 - PP_2865 0.58 +1.3 3,267,020 - PP_2865 0.58 -1.1 3,267,020 - PP_2865 0.58 +0.2 3,267,115 + PP_2865 0.69 +0.6 3,267,115 + PP_2865 0.69 -1.1 3,267,203 + PP_2865 0.79 +0.5 3,267,203 + PP_2865 0.79 -2.1 3,267,203 + PP_2865 0.79 +0.6 3,267,204 - PP_2865 0.79 +0.9 3,267,204 - PP_2865 0.79 -0.7 3,267,204 - PP_2865 0.79 +0.1 3,267,204 - PP_2865 0.79 -0.4 3,267,385 - -0.8 3,267,385 - +0.1
Or see this region's nucleotide sequence