Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2524 and PP_2525 are separated by 31 nucleotides PP_2525 and PP_2526 are separated by 146 nucleotides PP_2526 and PP_2527 are separated by 81 nucleotides
PP_2524: PP_2524 - HD domain protein, at 2,871,223 to 2,871,879
_2524
PP_2525: PP_2525 - conserved protein of unknown function, at 2,871,911 to 2,873,074
_2525
PP_2526: PP_2526 - conserved membrane protein of unknown function, at 2,873,221 to 2,873,694
_2526
PP_2527: PP_2527 - conserved membrane protein of unknown function, at 2,873,776 to 2,874,540
_2527
Position (kb)
2871
2872
2873
2874 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2870.928 kb on + strand at 2870.929 kb on - strand at 2870.929 kb on - strand at 2870.930 kb on + strand at 2870.930 kb on + strand at 2871.056 kb on - strand at 2871.056 kb on - strand at 2871.079 kb on + strand at 2871.120 kb on + strand at 2871.120 kb on + strand at 2871.153 kb on + strand at 2871.215 kb on + strand at 2871.215 kb on + strand at 2871.216 kb on - strand at 2871.247 kb on + strand at 2871.277 kb on + strand at 2871.278 kb on - strand at 2871.321 kb on + strand, within PP_2524 at 2871.440 kb on + strand at 2871.440 kb on + strand, within PP_2524 at 2871.441 kb on - strand, within PP_2524 at 2871.527 kb on + strand, within PP_2524 at 2871.528 kb on - strand, within PP_2524 at 2871.554 kb on - strand, within PP_2524 at 2871.652 kb on + strand, within PP_2524 at 2871.683 kb on - strand, within PP_2524 at 2871.683 kb on - strand, within PP_2524 at 2871.783 kb on + strand, within PP_2524 at 2871.784 kb on - strand, within PP_2524 at 2871.784 kb on - strand, within PP_2524 at 2871.894 kb on + strand at 2871.895 kb on - strand at 2871.992 kb on + strand at 2871.993 kb on - strand at 2872.050 kb on - strand, within PP_2525 at 2872.265 kb on + strand, within PP_2525 at 2872.266 kb on - strand, within PP_2525 at 2872.695 kb on - strand, within PP_2525 at 2873.046 kb on + strand at 2873.065 kb on + strand at 2873.066 kb on - strand at 2873.183 kb on + strand at 2873.183 kb on + strand at 2873.183 kb on + strand at 2873.184 kb on - strand at 2873.185 kb on + strand at 2873.186 kb on - strand at 2873.186 kb on - strand at 2873.448 kb on - strand, within PP_2526 at 2873.596 kb on + strand, within PP_2526 at 2873.596 kb on + strand, within PP_2526 at 2873.605 kb on + strand, within PP_2526 at 2873.665 kb on + strand at 2873.666 kb on - strand at 2873.666 kb on - strand at 2873.666 kb on - strand at 2873.891 kb on + strand, within PP_2527 at 2873.891 kb on + strand, within PP_2527
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 2,870,928 + +0.9 2,870,929 - -0.1 2,870,929 - +1.4 2,870,930 + -3.5 2,870,930 + +0.8 2,871,056 - -3.0 2,871,056 - +0.5 2,871,079 + +0.3 2,871,120 + -0.3 2,871,120 + -4.2 2,871,153 + +0.2 2,871,215 + -0.5 2,871,215 + -3.2 2,871,216 - +0.1 2,871,247 + +0.6 2,871,277 + +1.8 2,871,278 - +0.2 2,871,321 + PP_2524 0.15 +0.1 2,871,440 + -1.3 2,871,440 + PP_2524 0.33 +1.1 2,871,441 - PP_2524 0.33 -0.3 2,871,527 + PP_2524 0.46 +1.6 2,871,528 - PP_2524 0.46 -0.4 2,871,554 - PP_2524 0.50 +1.0 2,871,652 + PP_2524 0.65 -0.7 2,871,683 - PP_2524 0.70 -2.7 2,871,683 - PP_2524 0.70 -1.7 2,871,783 + PP_2524 0.85 -0.4 2,871,784 - PP_2524 0.85 +1.4 2,871,784 - PP_2524 0.85 -1.1 2,871,894 + +0.7 2,871,895 - +2.3 2,871,992 + +0.5 2,871,993 - -3.0 2,872,050 - PP_2525 0.12 -0.8 2,872,265 + PP_2525 0.30 -3.9 2,872,266 - PP_2525 0.30 -0.1 2,872,695 - PP_2525 0.67 -3.8 2,873,046 + -0.7 2,873,065 + -4.0 2,873,066 - +0.6 2,873,183 + -0.8 2,873,183 + +0.3 2,873,183 + -0.1 2,873,184 - -0.2 2,873,185 + -3.1 2,873,186 - -0.7 2,873,186 - -0.1 2,873,448 - PP_2526 0.48 +0.6 2,873,596 + PP_2526 0.79 -0.2 2,873,596 + PP_2526 0.79 +1.7 2,873,605 + PP_2526 0.81 -3.2 2,873,665 + -0.3 2,873,666 - +2.5 2,873,666 - +0.3 2,873,666 - +0.5 2,873,891 + PP_2527 0.15 +0.7 2,873,891 + PP_2527 0.15 +0.7
Or see this region's nucleotide sequence