Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1475 and PP_1476 are separated by 130 nucleotides PP_1476 and PP_1477 are separated by 8 nucleotides
PP_1475: PP_1475 - CAIB/BAIF family protein, at 1,679,601 to 1,680,800
_1475
PP_1476: PP_1476 - conserved protein of unknown function, at 1,680,931 to 1,681,473
_1476
PP_1477: PP_1477 - ssDNA-specific exonuclease RecJ, at 1,681,482 to 1,683,191
_1477
Position (kb) 1680
1681
1682 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1680.148 kb on - strand, within PP_1475 at 1680.190 kb on + strand, within PP_1475 at 1680.190 kb on + strand, within PP_1475 at 1680.344 kb on - strand, within PP_1475 at 1680.344 kb on - strand, within PP_1475 at 1680.638 kb on - strand, within PP_1475 at 1680.767 kb on + strand at 1680.768 kb on - strand at 1680.893 kb on - strand at 1680.953 kb on - strand at 1681.069 kb on + strand, within PP_1476 at 1681.069 kb on + strand, within PP_1476 at 1681.070 kb on - strand, within PP_1476 at 1681.082 kb on - strand, within PP_1476 at 1681.250 kb on - strand, within PP_1476 at 1681.276 kb on - strand, within PP_1476 at 1681.369 kb on - strand, within PP_1476 at 1681.369 kb on - strand, within PP_1476 at 1681.402 kb on - strand, within PP_1476 at 1681.402 kb on - strand at 1681.620 kb on + strand at 1681.783 kb on - strand, within PP_1477 at 1681.783 kb on - strand, within PP_1477 at 1681.813 kb on - strand, within PP_1477 at 1682.058 kb on + strand, within PP_1477 at 1682.058 kb on + strand, within PP_1477 at 1682.058 kb on + strand, within PP_1477 at 1682.059 kb on - strand, within PP_1477 at 1682.059 kb on - strand, within PP_1477 at 1682.059 kb on - strand, within PP_1477 at 1682.104 kb on - strand, within PP_1477
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 1,680,148 - PP_1475 0.46 +1.1 1,680,190 + PP_1475 0.49 +1.2 1,680,190 + PP_1475 0.49 -2.4 1,680,344 - PP_1475 0.62 -1.0 1,680,344 - PP_1475 0.62 +0.8 1,680,638 - PP_1475 0.86 +2.0 1,680,767 + -0.4 1,680,768 - +0.4 1,680,893 - +0.9 1,680,953 - -3.8 1,681,069 + PP_1476 0.25 +1.5 1,681,069 + PP_1476 0.25 -1.4 1,681,070 - PP_1476 0.26 +0.4 1,681,082 - PP_1476 0.28 +0.6 1,681,250 - PP_1476 0.59 +2.6 1,681,276 - PP_1476 0.64 +0.5 1,681,369 - PP_1476 0.81 +0.4 1,681,369 - PP_1476 0.81 +1.1 1,681,402 - PP_1476 0.87 +0.8 1,681,402 - +0.2 1,681,620 + +0.4 1,681,783 - PP_1477 0.18 +0.3 1,681,783 - PP_1477 0.18 -1.5 1,681,813 - PP_1477 0.19 -0.5 1,682,058 + PP_1477 0.34 -0.0 1,682,058 + PP_1477 0.34 -0.4 1,682,058 + PP_1477 0.34 -1.1 1,682,059 - PP_1477 0.34 +0.7 1,682,059 - PP_1477 0.34 +0.8 1,682,059 - PP_1477 0.34 -3.5 1,682,104 - PP_1477 0.36 +1.5
Or see this region's nucleotide sequence