Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0795 and PP_0796 are separated by 147 nucleotides PP_0796 and PP_0797 are separated by 72 nucleotides PP_0797 and PP_0798 overlap by 4 nucleotides
PP_0795: PP_0795 - fructose PTS permease - IIBC component, at 913,290 to 915,032
_0795
PP_0796: PP_0796 - conserved protein of unknown function, at 915,180 to 915,467
_0796
PP_0797: PP_0797 - conserved protein of unknown function, at 915,540 to 916,079
_0797
PP_0798: PP_0798 - GGDEF domain protein, at 916,076 to 917,566
_0798
Position (kb) 915
916
917 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 914.608 kb on - strand, within PP_0795 at 914.808 kb on - strand, within PP_0795 at 914.970 kb on + strand at 914.970 kb on + strand at 914.971 kb on - strand at 914.971 kb on - strand at 914.971 kb on - strand at 914.971 kb on - strand at 915.028 kb on + strand at 915.028 kb on + strand at 915.028 kb on + strand at 915.097 kb on + strand at 915.098 kb on - strand at 915.213 kb on + strand, within PP_0796 at 915.214 kb on - strand, within PP_0796 at 915.214 kb on - strand, within PP_0796 at 915.214 kb on - strand, within PP_0796 at 915.311 kb on - strand, within PP_0796 at 915.311 kb on - strand, within PP_0796 at 915.347 kb on - strand, within PP_0796 at 915.347 kb on - strand, within PP_0796 at 915.378 kb on + strand, within PP_0796 at 915.379 kb on - strand, within PP_0796 at 915.379 kb on - strand, within PP_0796 at 915.379 kb on - strand, within PP_0796 at 915.436 kb on + strand, within PP_0796 at 915.580 kb on - strand at 915.634 kb on + strand, within PP_0797 at 915.654 kb on + strand, within PP_0797 at 915.655 kb on - strand, within PP_0797 at 915.655 kb on - strand, within PP_0797 at 915.813 kb on - strand, within PP_0797 at 915.933 kb on + strand, within PP_0797 at 915.965 kb on - strand, within PP_0797 at 915.990 kb on + strand, within PP_0797 at 915.990 kb on + strand, within PP_0797 at 915.990 kb on + strand, within PP_0797 at 915.991 kb on - strand, within PP_0797 at 915.991 kb on - strand, within PP_0797 at 915.991 kb on - strand, within PP_0797 at 915.991 kb on - strand, within PP_0797 at 916.077 kb on + strand at 916.307 kb on + strand, within PP_0798 at 916.307 kb on + strand, within PP_0798 at 916.329 kb on + strand, within PP_0798 at 916.478 kb on - strand, within PP_0798 at 916.483 kb on + strand, within PP_0798 at 916.483 kb on + strand, within PP_0798 at 916.484 kb on - strand, within PP_0798 at 916.731 kb on - strand, within PP_0798 at 916.813 kb on + strand, within PP_0798 at 916.813 kb on + strand, within PP_0798 at 916.860 kb on + strand, within PP_0798 at 916.861 kb on - strand, within PP_0798 at 916.861 kb on - strand, within PP_0798 at 916.863 kb on + strand, within PP_0798 at 916.863 kb on + strand, within PP_0798 at 916.863 kb on + strand at 916.863 kb on + strand, within PP_0798 at 916.863 kb on + strand, within PP_0798 at 916.863 kb on + strand, within PP_0798 at 916.864 kb on - strand, within PP_0798 at 916.864 kb on - strand, within PP_0798 at 916.864 kb on - strand, within PP_0798 at 916.987 kb on - strand, within PP_0798 at 917.044 kb on + strand, within PP_0798 at 917.062 kb on + strand, within PP_0798 at 917.062 kb on + strand, within PP_0798 at 917.062 kb on + strand, within PP_0798 at 917.062 kb on + strand, within PP_0798 at 917.063 kb on - strand, within PP_0798
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 914,608 - PP_0795 0.76 +1.2 914,808 - PP_0795 0.87 -2.5 914,970 + +0.9 914,970 + -2.8 914,971 - -2.1 914,971 - +0.9 914,971 - +1.4 914,971 - -0.5 915,028 + -0.8 915,028 + +1.7 915,028 + +0.4 915,097 + +0.4 915,098 - +1.4 915,213 + PP_0796 0.11 +1.9 915,214 - PP_0796 0.12 -0.6 915,214 - PP_0796 0.12 +0.7 915,214 - PP_0796 0.12 -1.4 915,311 - PP_0796 0.45 +1.3 915,311 - PP_0796 0.45 -0.8 915,347 - PP_0796 0.58 -3.3 915,347 - PP_0796 0.58 +0.5 915,378 + PP_0796 0.69 +0.8 915,379 - PP_0796 0.69 +0.5 915,379 - PP_0796 0.69 -2.0 915,379 - PP_0796 0.69 +0.3 915,436 + PP_0796 0.89 -2.5 915,580 - +0.6 915,634 + PP_0797 0.17 -0.3 915,654 + PP_0797 0.21 +2.0 915,655 - PP_0797 0.21 +0.6 915,655 - PP_0797 0.21 -0.1 915,813 - PP_0797 0.51 +2.0 915,933 + PP_0797 0.73 -0.4 915,965 - PP_0797 0.79 -0.0 915,990 + PP_0797 0.83 +0.7 915,990 + PP_0797 0.83 +1.8 915,990 + PP_0797 0.83 -3.3 915,991 - PP_0797 0.84 +3.1 915,991 - PP_0797 0.84 -3.5 915,991 - PP_0797 0.84 +0.1 915,991 - PP_0797 0.84 +1.6 916,077 + +0.1 916,307 + PP_0798 0.15 -1.6 916,307 + PP_0798 0.15 +0.2 916,329 + PP_0798 0.17 +1.0 916,478 - PP_0798 0.27 -3.3 916,483 + PP_0798 0.27 +0.3 916,483 + PP_0798 0.27 -0.1 916,484 - PP_0798 0.27 -4.2 916,731 - PP_0798 0.44 -0.2 916,813 + PP_0798 0.49 +0.4 916,813 + PP_0798 0.49 -4.4 916,860 + PP_0798 0.53 -1.0 916,861 - PP_0798 0.53 -3.0 916,861 - PP_0798 0.53 -1.3 916,863 + PP_0798 0.53 -0.1 916,863 + PP_0798 0.53 -0.3 916,863 + -0.3 916,863 + PP_0798 0.53 +0.3 916,863 + PP_0798 0.53 -1.6 916,863 + PP_0798 0.53 -3.4 916,864 - PP_0798 0.53 +0.7 916,864 - PP_0798 0.53 +0.3 916,864 - PP_0798 0.53 -2.4 916,987 - PP_0798 0.61 +1.1 917,044 + PP_0798 0.65 +0.5 917,062 + PP_0798 0.66 -0.5 917,062 + PP_0798 0.66 +0.4 917,062 + PP_0798 0.66 +1.3 917,062 + PP_0798 0.66 +0.7 917,063 - PP_0798 0.66 -1.2
Or see this region's nucleotide sequence