Experiment: Growth at pH9 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4824 and PP_4825 are separated by 75 nucleotides PP_4825 and PP_5714 are separated by 10 nucleotides PP_5714 and PP_5715 overlap by 8 nucleotides
PP_4824: PP_4824 - Sensor histidine kinase/response regulator, at 5,486,672 to 5,489,443
_4824
PP_4825: PP_4825 - Membrane protein, MarC family, at 5,489,519 to 5,490,115
_4825
PP_5714: PP_5714 - conserved protein of unknown function, at 5,490,126 to 5,490,782
_5714
PP_5715: PP_5715 - protein of unknown function, at 5,490,775 to 5,491,464
_5715
Position (kb) 5489
5490
5491 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5488.641 kb on + strand, within PP_4824 at 5488.641 kb on + strand, within PP_4824 at 5488.933 kb on + strand, within PP_4824 at 5488.933 kb on + strand, within PP_4824 at 5488.933 kb on + strand at 5488.933 kb on + strand, within PP_4824 at 5488.933 kb on + strand at 5488.933 kb on + strand, within PP_4824 at 5488.934 kb on - strand, within PP_4824 at 5489.172 kb on - strand at 5489.172 kb on - strand at 5489.172 kb on - strand at 5489.298 kb on + strand at 5489.298 kb on + strand at 5489.299 kb on - strand at 5489.299 kb on - strand at 5489.299 kb on - strand at 5489.434 kb on + strand at 5489.434 kb on + strand at 5489.434 kb on + strand at 5489.434 kb on + strand at 5489.435 kb on - strand at 5489.435 kb on - strand at 5489.498 kb on + strand at 5489.499 kb on - strand at 5489.544 kb on - strand at 5489.564 kb on + strand at 5489.565 kb on - strand at 5489.565 kb on - strand at 5489.684 kb on + strand, within PP_4825 at 5489.968 kb on - strand, within PP_4825 at 5489.981 kb on + strand, within PP_4825 at 5489.981 kb on + strand, within PP_4825 at 5489.981 kb on + strand, within PP_4825 at 5489.981 kb on + strand, within PP_4825 at 5489.981 kb on + strand, within PP_4825 at 5489.981 kb on + strand, within PP_4825 at 5489.981 kb on + strand, within PP_4825 at 5489.981 kb on + strand, within PP_4825 at 5489.981 kb on + strand, within PP_4825 at 5489.982 kb on - strand, within PP_4825 at 5489.982 kb on - strand, within PP_4825 at 5489.982 kb on - strand, within PP_4825 at 5489.985 kb on + strand, within PP_4825 at 5489.986 kb on - strand, within PP_4825 at 5490.051 kb on + strand, within PP_4825 at 5490.052 kb on - strand, within PP_4825 at 5490.052 kb on - strand, within PP_4825 at 5490.052 kb on - strand, within PP_4825 at 5490.085 kb on + strand at 5490.102 kb on - strand at 5490.113 kb on + strand at 5490.127 kb on + strand at 5490.127 kb on + strand at 5490.147 kb on - strand at 5490.255 kb on + strand, within PP_5714 at 5490.256 kb on - strand, within PP_5714 at 5490.256 kb on - strand, within PP_5714 at 5490.256 kb on - strand, within PP_5714 at 5490.472 kb on + strand, within PP_5714 at 5490.472 kb on + strand, within PP_5714 at 5490.605 kb on - strand, within PP_5714 at 5490.708 kb on - strand, within PP_5714 at 5490.847 kb on - strand, within PP_5715 at 5490.899 kb on + strand at 5490.900 kb on - strand, within PP_5715 at 5490.988 kb on - strand, within PP_5715 at 5490.988 kb on - strand, within PP_5715 at 5491.027 kb on + strand, within PP_5715 at 5491.028 kb on - strand, within PP_5715 at 5491.028 kb on - strand, within PP_5715 at 5491.065 kb on + strand, within PP_5715 at 5491.066 kb on - strand, within PP_5715 at 5491.096 kb on + strand, within PP_5715 at 5491.096 kb on + strand, within PP_5715
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate remove 5,488,641 + PP_4824 0.71 -1.8 5,488,641 + PP_4824 0.71 -1.7 5,488,933 + PP_4824 0.82 +3.7 5,488,933 + PP_4824 0.82 -1.8 5,488,933 + +0.8 5,488,933 + PP_4824 0.82 +2.1 5,488,933 + -0.6 5,488,933 + PP_4824 0.82 -0.9 5,488,934 - PP_4824 0.82 -1.2 5,489,172 - +0.4 5,489,172 - +1.1 5,489,172 - -1.3 5,489,298 + -1.1 5,489,298 + -0.8 5,489,299 - +0.4 5,489,299 - -0.8 5,489,299 - +2.4 5,489,434 + +0.5 5,489,434 + -2.9 5,489,434 + +1.7 5,489,434 + +0.7 5,489,435 - +0.6 5,489,435 - -1.8 5,489,498 + +0.3 5,489,499 - -1.9 5,489,544 - -1.9 5,489,564 + -1.3 5,489,565 - +0.3 5,489,565 - -1.2 5,489,684 + PP_4825 0.28 -0.5 5,489,968 - PP_4825 0.75 -0.6 5,489,981 + PP_4825 0.77 +0.1 5,489,981 + PP_4825 0.77 -0.3 5,489,981 + PP_4825 0.77 +1.7 5,489,981 + PP_4825 0.77 -1.3 5,489,981 + PP_4825 0.77 +0.4 5,489,981 + PP_4825 0.77 -1.7 5,489,981 + PP_4825 0.77 +1.2 5,489,981 + PP_4825 0.77 +0.5 5,489,981 + PP_4825 0.77 -3.2 5,489,982 - PP_4825 0.78 -3.7 5,489,982 - PP_4825 0.78 +0.5 5,489,982 - PP_4825 0.78 +0.5 5,489,985 + PP_4825 0.78 -0.5 5,489,986 - PP_4825 0.78 +0.4 5,490,051 + PP_4825 0.89 -0.3 5,490,052 - PP_4825 0.89 +0.5 5,490,052 - PP_4825 0.89 -0.6 5,490,052 - PP_4825 0.89 -3.5 5,490,085 + -0.4 5,490,102 - -0.0 5,490,113 + +0.9 5,490,127 + +0.8 5,490,127 + +1.0 5,490,147 - +0.2 5,490,255 + PP_5714 0.20 +0.4 5,490,256 - PP_5714 0.20 -3.1 5,490,256 - PP_5714 0.20 -3.0 5,490,256 - PP_5714 0.20 +0.2 5,490,472 + PP_5714 0.53 +0.2 5,490,472 + PP_5714 0.53 -0.3 5,490,605 - PP_5714 0.73 -2.8 5,490,708 - PP_5714 0.89 +0.7 5,490,847 - PP_5715 0.10 -0.3 5,490,899 + +0.1 5,490,900 - PP_5715 0.18 -0.4 5,490,988 - PP_5715 0.31 +1.9 5,490,988 - PP_5715 0.31 +1.8 5,491,027 + PP_5715 0.37 +0.5 5,491,028 - PP_5715 0.37 +0.0 5,491,028 - PP_5715 0.37 -2.1 5,491,065 + PP_5715 0.42 -2.8 5,491,066 - PP_5715 0.42 +1.0 5,491,096 + PP_5715 0.47 +0.7 5,491,096 + PP_5715 0.47 +0.8
Or see this region's nucleotide sequence