Experiment: Papa dilution 10-1 cacl2 maltose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagH and yagI are separated by 4 nucleotides yagI and argF are separated by 138 nucleotides
b0271: yagH - CP4-6 prophage; predicted xylosidase/arabinosidase (NCBI), at 286,013 to 287,623
yagH
b0272: yagI - CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 287,628 to 288,386
yagI
b0273: argF - CP4-6 prophage; ornithine carbamoyltransferase 2, chain F (NCBI), at 288,525 to 289,529
argF
Position (kb) 287
288
289 Strain fitness (log2 ratio) -1
0
1
2 at 286.655 kb on - strand, within yagH at 286.692 kb on + strand, within yagH at 286.692 kb on + strand, within yagH at 286.739 kb on + strand, within yagH at 286.789 kb on + strand, within yagH at 287.037 kb on - strand, within yagH at 287.070 kb on - strand, within yagH at 287.150 kb on - strand, within yagH at 287.230 kb on - strand, within yagH at 287.249 kb on + strand, within yagH at 287.253 kb on - strand, within yagH at 287.287 kb on + strand, within yagH at 287.293 kb on - strand, within yagH at 287.293 kb on - strand, within yagH at 287.302 kb on + strand, within yagH at 287.302 kb on + strand, within yagH at 287.302 kb on + strand, within yagH at 287.302 kb on + strand, within yagH at 287.310 kb on - strand, within yagH at 287.373 kb on - strand, within yagH at 287.373 kb on - strand, within yagH at 287.459 kb on + strand, within yagH at 287.489 kb on + strand at 287.489 kb on + strand at 287.496 kb on - strand at 287.599 kb on - strand at 287.604 kb on + strand at 287.643 kb on - strand at 287.643 kb on - strand at 287.670 kb on - strand at 287.743 kb on - strand, within yagI at 287.766 kb on + strand, within yagI at 287.782 kb on + strand, within yagI at 287.870 kb on + strand, within yagI at 287.904 kb on + strand, within yagI at 287.914 kb on + strand, within yagI at 287.914 kb on + strand, within yagI at 287.955 kb on + strand, within yagI at 288.049 kb on + strand, within yagI at 288.049 kb on + strand, within yagI at 288.056 kb on - strand, within yagI at 288.056 kb on - strand, within yagI at 288.057 kb on - strand, within yagI at 288.057 kb on - strand, within yagI at 288.103 kb on + strand, within yagI at 288.271 kb on + strand, within yagI at 288.271 kb on + strand, within yagI at 288.274 kb on + strand, within yagI at 288.282 kb on - strand, within yagI at 288.282 kb on - strand, within yagI at 288.308 kb on - strand, within yagI at 288.339 kb on - strand at 288.470 kb on - strand at 288.470 kb on - strand at 288.476 kb on + strand at 288.480 kb on - strand at 288.501 kb on - strand at 288.501 kb on - strand at 288.536 kb on - strand at 288.592 kb on - strand at 288.617 kb on - strand at 288.750 kb on + strand, within argF at 288.750 kb on + strand, within argF at 288.758 kb on - strand, within argF at 288.809 kb on - strand, within argF at 288.820 kb on + strand, within argF at 288.820 kb on + strand, within argF at 289.058 kb on - strand, within argF at 289.099 kb on + strand, within argF at 289.099 kb on - strand, within argF at 289.107 kb on - strand, within argF at 289.107 kb on - strand, within argF at 289.193 kb on + strand, within argF at 289.193 kb on + strand, within argF at 289.207 kb on + strand, within argF at 289.207 kb on + strand, within argF
Per-strain Table
Position Strand Gene LocusTag Fraction Papa dilution 10-1 cacl2 maltose remove 286,655 - yagH b0271 0.40 +0.7 286,692 + yagH b0271 0.42 +1.0 286,692 + yagH b0271 0.42 +1.4 286,739 + yagH b0271 0.45 +0.8 286,789 + yagH b0271 0.48 +0.8 287,037 - yagH b0271 0.64 +1.0 287,070 - yagH b0271 0.66 -0.1 287,150 - yagH b0271 0.71 +0.6 287,230 - yagH b0271 0.76 +1.1 287,249 + yagH b0271 0.77 +0.5 287,253 - yagH b0271 0.77 +0.0 287,287 + yagH b0271 0.79 +0.3 287,293 - yagH b0271 0.79 +0.4 287,293 - yagH b0271 0.79 +0.6 287,302 + yagH b0271 0.80 +0.7 287,302 + yagH b0271 0.80 +0.6 287,302 + yagH b0271 0.80 +0.4 287,302 + yagH b0271 0.80 +1.6 287,310 - yagH b0271 0.81 -0.2 287,373 - yagH b0271 0.84 -0.1 287,373 - yagH b0271 0.84 +0.4 287,459 + yagH b0271 0.90 +0.9 287,489 + +1.5 287,489 + +1.5 287,496 - +0.8 287,599 - -0.1 287,604 + +0.9 287,643 - +1.3 287,643 - +0.5 287,670 - +0.8 287,743 - yagI b0272 0.15 +0.6 287,766 + yagI b0272 0.18 +0.4 287,782 + yagI b0272 0.20 +0.9 287,870 + yagI b0272 0.32 +0.4 287,904 + yagI b0272 0.36 -0.8 287,914 + yagI b0272 0.38 -0.4 287,914 + yagI b0272 0.38 -0.8 287,955 + yagI b0272 0.43 +0.2 288,049 + yagI b0272 0.55 +1.2 288,049 + yagI b0272 0.55 +0.6 288,056 - yagI b0272 0.56 +0.4 288,056 - yagI b0272 0.56 -0.1 288,057 - yagI b0272 0.57 +1.0 288,057 - yagI b0272 0.57 +0.5 288,103 + yagI b0272 0.63 +0.5 288,271 + yagI b0272 0.85 +0.4 288,271 + yagI b0272 0.85 +0.8 288,274 + yagI b0272 0.85 -0.0 288,282 - yagI b0272 0.86 -0.3 288,282 - yagI b0272 0.86 +0.2 288,308 - yagI b0272 0.90 +0.0 288,339 - +1.3 288,470 - -0.3 288,470 - +0.1 288,476 + +1.4 288,480 - -0.2 288,501 - -0.1 288,501 - +1.1 288,536 - +0.9 288,592 - -0.0 288,617 - +0.4 288,750 + argF b0273 0.22 +0.6 288,750 + argF b0273 0.22 +1.6 288,758 - argF b0273 0.23 +0.4 288,809 - argF b0273 0.28 +0.0 288,820 + argF b0273 0.29 +0.7 288,820 + argF b0273 0.29 +1.2 289,058 - argF b0273 0.53 +1.1 289,099 + argF b0273 0.57 +0.2 289,099 - argF b0273 0.57 +1.1 289,107 - argF b0273 0.58 +1.5 289,107 - argF b0273 0.58 +2.0 289,193 + argF b0273 0.66 +0.7 289,193 + argF b0273 0.66 +1.2 289,207 + argF b0273 0.68 +0.7 289,207 + argF b0273 0.68 +0.7
Or see this region's nucleotide sequence