Experiment: Growth at pH8 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3711 and PP_3712 are separated by 161 nucleotides PP_3712 and PP_3713 are separated by 116 nucleotides PP_3713 and PP_3714 are separated by 43 nucleotides PP_3714 and PP_3715 are separated by 21 nucleotides
PP_3711: PP_3711 - diguanylate cyclase, at 4,233,553 to 4,235,064
_3711
PP_3712: PP_3712 - conserved protein of unknown function, at 4,235,226 to 4,235,720
_3712
PP_3713: PP_3713 - Catechol 1,2-dioxygenase (EC 1.13.11.1) (from data) , at 4,235,837 to 4,236,772
_3713
PP_3714: PP_3714 - Muconolactone Delta-isomerase, at 4,236,816 to 4,237,106
_3714
PP_3715: PP_3715 - Muconate cycloisomerase 1, at 4,237,128 to 4,238,249
_3715
Position (kb)
4235
4236
4237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4234.889 kb on + strand, within PP_3711 at 4234.901 kb on + strand, within PP_3711 at 4234.902 kb on - strand, within PP_3711 at 4234.902 kb on - strand, within PP_3711 at 4234.974 kb on + strand at 4234.975 kb on - strand at 4234.975 kb on - strand at 4234.994 kb on + strand at 4234.995 kb on - strand at 4234.995 kb on - strand at 4234.995 kb on - strand at 4234.995 kb on - strand at 4234.995 kb on - strand at 4235.003 kb on + strand at 4235.004 kb on - strand at 4235.004 kb on - strand at 4235.033 kb on + strand at 4235.033 kb on + strand at 4235.033 kb on - strand at 4235.034 kb on - strand at 4235.071 kb on + strand at 4235.191 kb on + strand at 4235.191 kb on + strand at 4235.191 kb on + strand at 4235.198 kb on + strand at 4235.356 kb on + strand, within PP_3712 at 4235.431 kb on + strand, within PP_3712 at 4235.593 kb on + strand, within PP_3712 at 4235.594 kb on - strand, within PP_3712 at 4235.644 kb on + strand, within PP_3712 at 4235.645 kb on - strand, within PP_3712 at 4235.650 kb on + strand, within PP_3712 at 4235.650 kb on + strand, within PP_3712 at 4235.651 kb on - strand, within PP_3712 at 4235.651 kb on - strand, within PP_3712 at 4235.651 kb on - strand, within PP_3712 at 4235.660 kb on - strand, within PP_3712 at 4235.751 kb on + strand at 4235.751 kb on + strand at 4235.826 kb on + strand at 4235.826 kb on + strand at 4235.827 kb on - strand at 4235.827 kb on - strand at 4236.018 kb on + strand, within PP_3713 at 4236.039 kb on + strand, within PP_3713 at 4236.279 kb on + strand at 4236.279 kb on + strand, within PP_3713 at 4236.280 kb on - strand, within PP_3713 at 4236.286 kb on - strand, within PP_3713 at 4236.286 kb on - strand, within PP_3713 at 4236.444 kb on + strand, within PP_3713 at 4236.444 kb on + strand, within PP_3713 at 4236.444 kb on + strand, within PP_3713 at 4236.444 kb on + strand, within PP_3713 at 4236.444 kb on + strand, within PP_3713 at 4236.444 kb on + strand, within PP_3713 at 4236.445 kb on - strand, within PP_3713 at 4236.779 kb on + strand at 4236.779 kb on + strand at 4236.779 kb on + strand at 4236.780 kb on - strand at 4236.786 kb on + strand at 4236.786 kb on + strand at 4236.786 kb on + strand at 4236.787 kb on - strand at 4236.787 kb on - strand at 4236.787 kb on - strand at 4236.787 kb on - strand at 4236.804 kb on + strand at 4236.804 kb on + strand at 4236.804 kb on + strand at 4236.804 kb on + strand at 4236.804 kb on + strand at 4236.804 kb on + strand at 4236.805 kb on - strand at 4236.805 kb on - strand at 4236.805 kb on - strand at 4236.805 kb on - strand at 4236.880 kb on + strand, within PP_3714 at 4236.880 kb on + strand, within PP_3714 at 4236.880 kb on + strand, within PP_3714 at 4236.881 kb on - strand, within PP_3714 at 4236.881 kb on - strand, within PP_3714 at 4236.949 kb on + strand, within PP_3714 at 4236.949 kb on + strand, within PP_3714 at 4236.949 kb on + strand, within PP_3714 at 4236.950 kb on - strand, within PP_3714 at 4236.950 kb on - strand, within PP_3714 at 4237.110 kb on + strand at 4237.111 kb on - strand at 4237.111 kb on - strand at 4237.358 kb on - strand, within PP_3715 at 4237.609 kb on - strand, within PP_3715 at 4237.645 kb on + strand, within PP_3715 at 4237.646 kb on - strand, within PP_3715 at 4237.646 kb on - strand, within PP_3715
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) Trisodium citrate remove 4,234,889 + PP_3711 0.88 -0.3 4,234,901 + PP_3711 0.89 -3.0 4,234,902 - PP_3711 0.89 +2.1 4,234,902 - PP_3711 0.89 +0.7 4,234,974 + -0.3 4,234,975 - -1.4 4,234,975 - +0.3 4,234,994 + +0.6 4,234,995 - +0.5 4,234,995 - +1.1 4,234,995 - -0.1 4,234,995 - +0.6 4,234,995 - -1.5 4,235,003 + -0.5 4,235,004 - -3.5 4,235,004 - -2.1 4,235,033 + -1.4 4,235,033 + +0.4 4,235,033 - -0.2 4,235,034 - +0.5 4,235,071 + +1.4 4,235,191 + +0.7 4,235,191 + -0.8 4,235,191 + -1.0 4,235,198 + -1.5 4,235,356 + PP_3712 0.26 -0.1 4,235,431 + PP_3712 0.41 -1.4 4,235,593 + PP_3712 0.74 +0.2 4,235,594 - PP_3712 0.74 +0.6 4,235,644 + PP_3712 0.84 -0.9 4,235,645 - PP_3712 0.85 -1.2 4,235,650 + PP_3712 0.86 +0.1 4,235,650 + PP_3712 0.86 -0.8 4,235,651 - PP_3712 0.86 +1.3 4,235,651 - PP_3712 0.86 -0.7 4,235,651 - PP_3712 0.86 -1.2 4,235,660 - PP_3712 0.88 -0.3 4,235,751 + -1.0 4,235,751 + -1.0 4,235,826 + -0.1 4,235,826 + +0.6 4,235,827 - -1.0 4,235,827 - -0.5 4,236,018 + PP_3713 0.19 -1.8 4,236,039 + PP_3713 0.22 -1.1 4,236,279 + -0.1 4,236,279 + PP_3713 0.47 -1.6 4,236,280 - PP_3713 0.47 -0.6 4,236,286 - PP_3713 0.48 +0.3 4,236,286 - PP_3713 0.48 -0.4 4,236,444 + PP_3713 0.65 +0.0 4,236,444 + PP_3713 0.65 +1.0 4,236,444 + PP_3713 0.65 +0.2 4,236,444 + PP_3713 0.65 -1.4 4,236,444 + PP_3713 0.65 +0.1 4,236,444 + PP_3713 0.65 -0.5 4,236,445 - PP_3713 0.65 -0.6 4,236,779 + +1.6 4,236,779 + -1.1 4,236,779 + +0.8 4,236,780 - +0.2 4,236,786 + -2.1 4,236,786 + -0.9 4,236,786 + -0.5 4,236,787 - +0.8 4,236,787 - +0.6 4,236,787 - +0.4 4,236,787 - +0.5 4,236,804 + -0.1 4,236,804 + +1.1 4,236,804 + -0.4 4,236,804 + -2.7 4,236,804 + -0.5 4,236,804 + -0.7 4,236,805 - -0.5 4,236,805 - +2.1 4,236,805 - -0.1 4,236,805 - +0.0 4,236,880 + PP_3714 0.22 -0.1 4,236,880 + PP_3714 0.22 +1.0 4,236,880 + PP_3714 0.22 -0.3 4,236,881 - PP_3714 0.22 -1.4 4,236,881 - PP_3714 0.22 +0.8 4,236,949 + PP_3714 0.46 -0.1 4,236,949 + PP_3714 0.46 +2.1 4,236,949 + PP_3714 0.46 -0.4 4,236,950 - PP_3714 0.46 -0.6 4,236,950 - PP_3714 0.46 +0.5 4,237,110 + +0.8 4,237,111 - +0.4 4,237,111 - -0.1 4,237,358 - PP_3715 0.20 -0.8 4,237,609 - PP_3715 0.43 -0.1 4,237,645 + PP_3715 0.46 -1.0 4,237,646 - PP_3715 0.46 +1.4 4,237,646 - PP_3715 0.46 +1.9
Or see this region's nucleotide sequence