Experiment: Growth at pH8 and (C) Trisodium citrate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0146 and PP_0147 are separated by 42 nucleotides PP_0147 and PP_0148 are separated by 264 nucleotides PP_0148 and PP_0149 are separated by 38 nucleotides
PP_0146: PP_0146 - putative transport protein, at 155,578 to 156,375
_0146
PP_0147: PP_0147 - Citrate transporter, at 156,418 to 157,725
_0147
PP_0148: PP_0148 - conserved protein of unknown function, at 157,990 to 158,394
_0148
PP_0149: PP_0149 - conserved exported protein of unknown function, at 158,433 to 160,388
_0149
Position (kb) 156
157
158 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 155.427 kb on - strand at 155.531 kb on - strand at 155.531 kb on - strand at 155.702 kb on + strand, within PP_0146 at 155.702 kb on + strand, within PP_0146 at 155.702 kb on + strand, within PP_0146 at 155.708 kb on + strand, within PP_0146 at 155.708 kb on + strand, within PP_0146 at 155.708 kb on + strand, within PP_0146 at 155.708 kb on + strand, within PP_0146 at 155.708 kb on + strand, within PP_0146 at 155.709 kb on - strand, within PP_0146 at 155.709 kb on - strand, within PP_0146 at 155.709 kb on - strand, within PP_0146 at 155.749 kb on + strand, within PP_0146 at 155.749 kb on + strand, within PP_0146 at 155.750 kb on - strand, within PP_0146 at 155.754 kb on + strand, within PP_0146 at 155.885 kb on - strand, within PP_0146 at 155.966 kb on + strand, within PP_0146 at 155.966 kb on + strand, within PP_0146 at 155.966 kb on + strand, within PP_0146 at 155.966 kb on + strand, within PP_0146 at 155.967 kb on - strand, within PP_0146 at 155.967 kb on - strand, within PP_0146 at 155.967 kb on - strand at 155.967 kb on - strand, within PP_0146 at 156.001 kb on + strand, within PP_0146 at 156.001 kb on + strand, within PP_0146 at 156.001 kb on + strand, within PP_0146 at 156.002 kb on - strand, within PP_0146 at 156.032 kb on + strand, within PP_0146 at 156.032 kb on + strand, within PP_0146 at 156.033 kb on - strand, within PP_0146 at 156.033 kb on - strand, within PP_0146 at 156.033 kb on - strand, within PP_0146 at 156.033 kb on - strand, within PP_0146 at 156.153 kb on + strand, within PP_0146 at 156.153 kb on + strand, within PP_0146 at 156.174 kb on + strand, within PP_0146 at 156.175 kb on - strand, within PP_0146 at 156.175 kb on - strand, within PP_0146 at 156.300 kb on + strand at 156.351 kb on - strand at 156.355 kb on + strand at 156.368 kb on - strand at 156.408 kb on + strand at 156.409 kb on - strand at 156.416 kb on + strand at 156.417 kb on - strand at 156.417 kb on - strand at 156.417 kb on - strand at 156.417 kb on - strand at 156.419 kb on + strand at 156.419 kb on + strand at 156.419 kb on + strand at 156.419 kb on + strand at 156.420 kb on - strand at 156.420 kb on - strand at 156.426 kb on + strand at 156.427 kb on - strand at 156.469 kb on + strand at 156.469 kb on + strand at 156.470 kb on - strand at 156.470 kb on - strand at 156.481 kb on + strand at 156.481 kb on + strand at 156.481 kb on + strand at 156.481 kb on + strand at 156.482 kb on - strand at 156.554 kb on + strand, within PP_0147 at 156.554 kb on + strand, within PP_0147 at 156.554 kb on + strand, within PP_0147 at 156.554 kb on + strand, within PP_0147 at 156.555 kb on - strand, within PP_0147 at 156.639 kb on - strand, within PP_0147 at 156.677 kb on + strand, within PP_0147 at 156.678 kb on - strand, within PP_0147 at 156.680 kb on + strand, within PP_0147 at 156.680 kb on + strand, within PP_0147 at 156.681 kb on - strand, within PP_0147 at 156.746 kb on + strand, within PP_0147 at 156.747 kb on - strand, within PP_0147 at 156.747 kb on - strand, within PP_0147 at 156.868 kb on + strand, within PP_0147 at 156.868 kb on + strand, within PP_0147 at 156.868 kb on + strand, within PP_0147 at 156.868 kb on + strand, within PP_0147 at 156.920 kb on + strand, within PP_0147 at 156.920 kb on + strand, within PP_0147 at 156.920 kb on + strand, within PP_0147 at 156.921 kb on - strand, within PP_0147 at 156.921 kb on - strand, within PP_0147 at 156.921 kb on - strand, within PP_0147 at 157.028 kb on + strand, within PP_0147 at 157.028 kb on + strand, within PP_0147 at 157.029 kb on - strand, within PP_0147 at 157.029 kb on - strand, within PP_0147 at 157.142 kb on + strand, within PP_0147 at 157.142 kb on + strand, within PP_0147 at 157.142 kb on + strand, within PP_0147 at 157.142 kb on + strand, within PP_0147 at 157.142 kb on + strand, within PP_0147 at 157.142 kb on + strand, within PP_0147 at 157.143 kb on - strand, within PP_0147 at 157.143 kb on - strand, within PP_0147 at 157.143 kb on - strand, within PP_0147 at 157.148 kb on + strand, within PP_0147 at 157.148 kb on + strand, within PP_0147 at 157.149 kb on - strand, within PP_0147 at 157.149 kb on - strand, within PP_0147 at 157.149 kb on - strand, within PP_0147 at 157.149 kb on - strand, within PP_0147 at 157.172 kb on - strand, within PP_0147 at 157.201 kb on + strand, within PP_0147 at 157.223 kb on - strand, within PP_0147 at 157.340 kb on + strand at 157.340 kb on + strand, within PP_0147 at 157.340 kb on + strand, within PP_0147 at 157.341 kb on - strand, within PP_0147 at 157.341 kb on - strand, within PP_0147 at 157.373 kb on + strand, within PP_0147 at 157.373 kb on + strand, within PP_0147 at 157.373 kb on + strand, within PP_0147 at 157.373 kb on + strand, within PP_0147 at 157.373 kb on + strand, within PP_0147 at 157.373 kb on + strand, within PP_0147 at 157.374 kb on - strand, within PP_0147 at 157.374 kb on - strand, within PP_0147 at 157.374 kb on - strand, within PP_0147 at 157.418 kb on - strand, within PP_0147 at 157.520 kb on + strand, within PP_0147 at 157.521 kb on - strand, within PP_0147 at 157.577 kb on - strand, within PP_0147 at 157.682 kb on + strand at 157.682 kb on + strand at 157.682 kb on + strand at 157.682 kb on + strand at 157.683 kb on - strand at 157.731 kb on + strand at 157.731 kb on + strand at 157.731 kb on + strand at 157.731 kb on + strand at 157.732 kb on - strand at 157.817 kb on + strand at 157.839 kb on + strand at 157.839 kb on + strand at 157.839 kb on + strand at 157.839 kb on + strand at 157.839 kb on + strand at 157.839 kb on + strand at 157.839 kb on + strand at 157.840 kb on - strand at 157.840 kb on - strand at 157.840 kb on - strand at 157.840 kb on - strand at 157.840 kb on - strand at 157.922 kb on - strand at 157.936 kb on + strand at 157.936 kb on + strand at 157.936 kb on + strand at 157.987 kb on + strand at 157.987 kb on + strand at 157.988 kb on - strand at 158.004 kb on - strand at 158.004 kb on - strand at 158.175 kb on + strand, within PP_0148 at 158.176 kb on + strand, within PP_0148 at 158.176 kb on + strand, within PP_0148 at 158.176 kb on + strand, within PP_0148 at 158.176 kb on + strand, within PP_0148 at 158.176 kb on + strand, within PP_0148 at 158.176 kb on + strand, within PP_0148 at 158.176 kb on + strand, within PP_0148 at 158.177 kb on - strand, within PP_0148 at 158.177 kb on - strand, within PP_0148 at 158.177 kb on - strand, within PP_0148 at 158.177 kb on - strand, within PP_0148 at 158.203 kb on + strand, within PP_0148 at 158.251 kb on - strand, within PP_0148 at 158.304 kb on - strand, within PP_0148 at 158.319 kb on + strand, within PP_0148 at 158.319 kb on + strand at 158.319 kb on + strand, within PP_0148 at 158.319 kb on + strand, within PP_0148 at 158.319 kb on + strand, within PP_0148 at 158.320 kb on - strand, within PP_0148 at 158.320 kb on - strand, within PP_0148 at 158.320 kb on - strand, within PP_0148 at 158.320 kb on - strand, within PP_0148 at 158.320 kb on - strand, within PP_0148 at 158.393 kb on - strand at 158.429 kb on + strand at 158.429 kb on + strand at 158.429 kb on + strand at 158.429 kb on + strand at 158.429 kb on + strand at 158.430 kb on - strand at 158.430 kb on - strand at 158.430 kb on - strand at 158.430 kb on - strand at 158.430 kb on - strand at 158.430 kb on - strand at 158.430 kb on - strand at 158.430 kb on - strand at 158.430 kb on - strand at 158.430 kb on - strand at 158.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH8 and (C) Trisodium citrate remove 155,427 - -0.5 155,531 - -0.2 155,531 - -0.2 155,702 + PP_0146 0.16 -2.5 155,702 + PP_0146 0.16 -1.0 155,702 + PP_0146 0.16 -1.0 155,708 + PP_0146 0.16 -0.1 155,708 + PP_0146 0.16 +0.8 155,708 + PP_0146 0.16 +0.2 155,708 + PP_0146 0.16 -0.1 155,708 + PP_0146 0.16 +1.5 155,709 - PP_0146 0.16 +0.1 155,709 - PP_0146 0.16 -0.2 155,709 - PP_0146 0.16 -1.5 155,749 + PP_0146 0.21 +2.4 155,749 + PP_0146 0.21 +2.0 155,750 - PP_0146 0.22 -1.5 155,754 + PP_0146 0.22 +0.3 155,885 - PP_0146 0.38 -0.0 155,966 + PP_0146 0.49 -0.8 155,966 + PP_0146 0.49 +1.0 155,966 + PP_0146 0.49 -0.4 155,966 + PP_0146 0.49 +0.8 155,967 - PP_0146 0.49 +0.3 155,967 - PP_0146 0.49 -0.7 155,967 - +0.2 155,967 - PP_0146 0.49 +0.1 156,001 + PP_0146 0.53 -0.3 156,001 + PP_0146 0.53 -0.5 156,001 + PP_0146 0.53 -0.2 156,002 - PP_0146 0.53 -0.8 156,032 + PP_0146 0.57 -1.1 156,032 + PP_0146 0.57 -0.2 156,033 - PP_0146 0.57 -0.4 156,033 - PP_0146 0.57 -0.0 156,033 - PP_0146 0.57 -0.2 156,033 - PP_0146 0.57 +0.5 156,153 + PP_0146 0.72 -0.8 156,153 + PP_0146 0.72 -0.1 156,174 + PP_0146 0.75 -0.7 156,175 - PP_0146 0.75 +0.9 156,175 - PP_0146 0.75 +1.4 156,300 + +1.2 156,351 - +0.8 156,355 + +1.8 156,368 - +0.8 156,408 + +1.1 156,409 - +0.0 156,416 + -0.1 156,417 - -0.5 156,417 - +0.5 156,417 - +0.2 156,417 - -1.9 156,419 + +0.0 156,419 + +0.8 156,419 + -0.1 156,419 + -1.9 156,420 - -1.1 156,420 - -1.8 156,426 + -0.1 156,427 - -0.7 156,469 + +1.1 156,469 + -2.4 156,470 - -0.8 156,470 - -0.2 156,481 + -0.0 156,481 + -0.7 156,481 + -1.2 156,481 + -1.0 156,482 - -2.9 156,554 + PP_0147 0.10 -0.3 156,554 + PP_0147 0.10 -2.6 156,554 + PP_0147 0.10 -0.9 156,554 + PP_0147 0.10 -0.6 156,555 - PP_0147 0.10 -2.5 156,639 - PP_0147 0.17 -0.7 156,677 + PP_0147 0.20 +0.3 156,678 - PP_0147 0.20 -3.8 156,680 + PP_0147 0.20 -1.8 156,680 + PP_0147 0.20 -1.6 156,681 - PP_0147 0.20 -3.3 156,746 + PP_0147 0.25 -0.7 156,747 - PP_0147 0.25 -1.7 156,747 - PP_0147 0.25 -0.3 156,868 + PP_0147 0.34 +0.1 156,868 + PP_0147 0.34 -0.0 156,868 + PP_0147 0.34 -3.6 156,868 + PP_0147 0.34 -1.5 156,920 + PP_0147 0.38 -0.1 156,920 + PP_0147 0.38 +0.2 156,920 + PP_0147 0.38 -1.4 156,921 - PP_0147 0.38 -1.4 156,921 - PP_0147 0.38 -2.3 156,921 - PP_0147 0.38 -0.5 157,028 + PP_0147 0.47 +0.1 157,028 + PP_0147 0.47 -0.8 157,029 - PP_0147 0.47 +0.6 157,029 - PP_0147 0.47 -0.3 157,142 + PP_0147 0.55 -3.6 157,142 + PP_0147 0.55 +0.3 157,142 + PP_0147 0.55 -2.2 157,142 + PP_0147 0.55 +0.1 157,142 + PP_0147 0.55 -3.8 157,142 + PP_0147 0.55 -1.2 157,143 - PP_0147 0.55 -2.4 157,143 - PP_0147 0.55 -3.7 157,143 - PP_0147 0.55 -1.9 157,148 + PP_0147 0.56 +0.5 157,148 + PP_0147 0.56 -0.7 157,149 - PP_0147 0.56 -3.0 157,149 - PP_0147 0.56 -3.1 157,149 - PP_0147 0.56 -2.6 157,149 - PP_0147 0.56 -1.4 157,172 - PP_0147 0.58 -0.5 157,201 + PP_0147 0.60 -0.5 157,223 - PP_0147 0.62 -0.4 157,340 + -0.8 157,340 + PP_0147 0.70 +0.2 157,340 + PP_0147 0.70 -1.7 157,341 - PP_0147 0.71 -0.9 157,341 - PP_0147 0.71 -0.9 157,373 + PP_0147 0.73 -2.6 157,373 + PP_0147 0.73 -2.4 157,373 + PP_0147 0.73 -0.3 157,373 + PP_0147 0.73 -0.7 157,373 + PP_0147 0.73 -0.5 157,373 + PP_0147 0.73 +0.3 157,374 - PP_0147 0.73 -0.7 157,374 - PP_0147 0.73 -3.1 157,374 - PP_0147 0.73 -1.6 157,418 - PP_0147 0.76 -0.1 157,520 + PP_0147 0.84 +0.1 157,521 - PP_0147 0.84 +1.0 157,577 - PP_0147 0.89 +0.0 157,682 + -1.0 157,682 + +0.1 157,682 + -0.0 157,682 + -0.6 157,683 - -3.4 157,731 + -2.0 157,731 + -3.1 157,731 + -1.5 157,731 + -1.2 157,732 - -0.9 157,817 + -1.8 157,839 + -1.6 157,839 + -0.1 157,839 + +0.1 157,839 + -3.3 157,839 + -0.6 157,839 + -0.7 157,839 + -0.2 157,840 - -0.3 157,840 - -2.5 157,840 - -0.3 157,840 - -3.5 157,840 - -1.4 157,922 - -1.8 157,936 + -1.5 157,936 + -0.3 157,936 + -3.9 157,987 + -0.2 157,987 + -0.3 157,988 - +1.4 158,004 - +1.9 158,004 - +0.8 158,175 + PP_0148 0.46 -0.2 158,176 + PP_0148 0.46 +0.6 158,176 + PP_0148 0.46 -1.6 158,176 + PP_0148 0.46 -0.5 158,176 + PP_0148 0.46 +1.2 158,176 + PP_0148 0.46 -1.5 158,176 + PP_0148 0.46 +0.1 158,176 + PP_0148 0.46 -2.5 158,177 - PP_0148 0.46 +0.1 158,177 - PP_0148 0.46 -0.6 158,177 - PP_0148 0.46 -0.2 158,177 - PP_0148 0.46 -0.3 158,203 + PP_0148 0.53 +0.0 158,251 - PP_0148 0.64 -0.3 158,304 - PP_0148 0.78 -2.2 158,319 + PP_0148 0.81 +0.0 158,319 + +3.2 158,319 + PP_0148 0.81 -0.0 158,319 + PP_0148 0.81 +0.6 158,319 + PP_0148 0.81 -3.5 158,320 - PP_0148 0.81 +1.2 158,320 - PP_0148 0.81 -0.7 158,320 - PP_0148 0.81 -0.4 158,320 - PP_0148 0.81 +0.2 158,320 - PP_0148 0.81 -0.6 158,393 - -0.3 158,429 + +0.9 158,429 + -0.3 158,429 + -0.3 158,429 + -0.6 158,429 + -0.2 158,430 - +0.7 158,430 - -1.6 158,430 - -0.1 158,430 - -0.2 158,430 - -2.5 158,430 - +0.9 158,430 - +0.7 158,430 - +0.5 158,430 - -1.8 158,430 - -0.7 158,430 - +0.6
Or see this region's nucleotide sequence