Experiment: P2 dilution 10-3 cacl2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjjA and dnaC are separated by 48 nucleotides dnaC and dnaT are separated by 2 nucleotides dnaT and yjjB are separated by 106 nucleotides yjjB and yjjP overlap by 10 nucleotides
b4360: yjjA - putative glycoprotein/receptor (VIMSS), at 4,597,718 to 4,598,212
yjjA
b4361: dnaC - DNA biosynthesis protein (NCBI), at 4,598,261 to 4,598,998
dnaC
b4362: dnaT - primosomal protein I (NCBI), at 4,599,001 to 4,599,540
dnaT
b4363: yjjB - conserved inner membrane protein (RefSeq), at 4,599,647 to 4,600,120
yjjB
b4364: yjjP - putative structural protein (VIMSS), at 4,600,111 to 4,600,881
yjjP
Position (kb)
4599
4600 Strain fitness (log2 ratio)
-1
0
1
2 at 4598.117 kb on - strand, within yjjA at 4598.220 kb on - strand at 4598.220 kb on - strand at 4598.235 kb on - strand at 4598.240 kb on - strand at 4598.240 kb on - strand at 4599.536 kb on + strand at 4599.561 kb on - strand at 4599.589 kb on + strand at 4599.593 kb on + strand at 4599.609 kb on - strand at 4599.609 kb on - strand at 4599.609 kb on - strand at 4599.619 kb on + strand at 4599.681 kb on - strand at 4599.761 kb on - strand, within yjjB at 4599.761 kb on - strand, within yjjB at 4599.831 kb on + strand, within yjjB at 4599.837 kb on - strand, within yjjB at 4599.873 kb on + strand, within yjjB at 4599.873 kb on + strand, within yjjB at 4599.874 kb on + strand, within yjjB at 4599.874 kb on + strand, within yjjB at 4599.941 kb on + strand, within yjjB at 4600.053 kb on - strand, within yjjB at 4600.138 kb on - strand at 4600.198 kb on + strand, within yjjP at 4600.208 kb on - strand, within yjjP at 4600.211 kb on + strand, within yjjP at 4600.219 kb on - strand, within yjjP at 4600.219 kb on - strand, within yjjP at 4600.273 kb on + strand, within yjjP at 4600.273 kb on + strand, within yjjP at 4600.455 kb on + strand, within yjjP at 4600.485 kb on - strand, within yjjP at 4600.485 kb on - strand, within yjjP at 4600.538 kb on + strand, within yjjP at 4600.538 kb on + strand, within yjjP
Per-strain Table
Position Strand Gene LocusTag Fraction P2 dilution 10-3 cacl2 remove 4,598,117 - yjjA b4360 0.81 +0.3 4,598,220 - -0.1 4,598,220 - +0.0 4,598,235 - +0.1 4,598,240 - -0.2 4,598,240 - +1.4 4,599,536 + +0.7 4,599,561 - +0.1 4,599,589 + -1.1 4,599,593 + +0.2 4,599,609 - +0.3 4,599,609 - +0.6 4,599,609 - +0.2 4,599,619 + +0.1 4,599,681 - +0.2 4,599,761 - yjjB b4363 0.24 -0.6 4,599,761 - yjjB b4363 0.24 +0.6 4,599,831 + yjjB b4363 0.39 -0.1 4,599,837 - yjjB b4363 0.40 -0.2 4,599,873 + yjjB b4363 0.48 -0.9 4,599,873 + yjjB b4363 0.48 -1.0 4,599,874 + yjjB b4363 0.48 +0.2 4,599,874 + yjjB b4363 0.48 +1.9 4,599,941 + yjjB b4363 0.62 -0.2 4,600,053 - yjjB b4363 0.86 +0.1 4,600,138 - -0.2 4,600,198 + yjjP b4364 0.11 +0.0 4,600,208 - yjjP b4364 0.13 -0.3 4,600,211 + yjjP b4364 0.13 +0.0 4,600,219 - yjjP b4364 0.14 +0.2 4,600,219 - yjjP b4364 0.14 -0.3 4,600,273 + yjjP b4364 0.21 +0.5 4,600,273 + yjjP b4364 0.21 -0.0 4,600,455 + yjjP b4364 0.45 +0.8 4,600,485 - yjjP b4364 0.49 +0.1 4,600,485 - yjjP b4364 0.49 -0.3 4,600,538 + yjjP b4364 0.55 +0.2 4,600,538 + yjjP b4364 0.55 +0.5
Or see this region's nucleotide sequence